Nucleic acid agents for overexpressing or downregulating RNA interference targets and uses of same in improving nitrogen use efficiency, abiotic stress tolerance, biomass, vigor or yield of a plant

ABSTRACT

A method of improving nitrogen use efficiency, abiotic stress tolerance, biomass, vigor or yield of a plant is provided by expressing within the plant an exogenous polynucleotide encoding a polypeptide having an amino acid sequence at least 80% homologous to SEQ ID NOs: 687-981, 992-1248, 1281-1310, 1389-1391, and 2806-3081. Also provided is a method of improving nitrogen use efficiency, abiotic stress tolerance, biomass, vigor or yield of a plan by expressing within the plant an exogenous polynucleotide which downregulates an activity or expression of a polypeptide having an amino acid sequence at least 80% homologous to SEQ ID NOs: 311-514, 2007-2436, 1311-1320, 982-991, 1249-1280, 1321-1388. Transgenic plants and constructs are provided as well.

RELATED APPLICATIONS

This application is a National Phase of PCT Patent Application No. PCT/IB2012/054149 having International filing date of Aug. 14, 2012, which claims the benefit of priority under 35 USC § 119(e) of U.S. Provisional Patent Application No. 61/523,355 filed on Aug. 14, 2011. The contents of the above applications are all incorporated by reference as if fully set forth herein in their entirety.

SEQUENCE LISTING STATEMENT

The ASCII file, entitled 58604SequenceListing.txt, created on Feb. 13, 2014, comprising 9,493,532 bytes, submitted concurrently with the filing of this application is incorporated herein by reference.

FIELD AND BACKGROUND OF THE INVENTION

The present invention, in some embodiments thereof, relates to nucleic acid agents for overexpressing or downregulating RNA interference targets and uses of same in improving nitrogen use efficiency, abiotic stress tolerance, biomass, vigor or yield of a plant.

Plant growth is reliant on a number of basic factors: light, air, water, nutrients, and physical support. All these factors, with the exception of light, are controlled by soil to some extent, which integrates non-living substances (minerals, organic matter, gases and liquids) and living organisms (bacteria, fungi, insects, worms, etc.). The soil's volume is almost equally divided between solids and water/gases. An adequate nutrition in the form of natural as well as synthetic fertilizers, may affect crop yield and quality, and its response to stress factors such as disease and adverse weather. The great importance of fertilizers can best be appreciated when considering the direct increase in crop yields over the last 40 years, and the fact that they account for most of the overhead expense in agriculture. Sixteen natural nutrients are essential for plant growth, three of which, carbon, hydrogen and oxygen, are retrieved from air and water. The soil provides the remaining 13 nutrients.

Nutrients are naturally recycled within a self-sufficient environment, such as a rainforest. However, when grown in a commercial situation, plants consume nutrients for their growth and these nutrients need to be replenished in the system. Several nutrients are consumed by plants in large quantities and are referred to as macronutrients. Three macronutrients are considered the basic building blocks of plant growth, and are provided as main fertilizers; Nitrogen (N), Phosphate (P) and Potassium (K). Yet, only nitrogen needs to be replenished every year since plants only absorb approximately half of the nitrogen fertilizer applied. A proper balance of nutrients is crucial; when too much of an essential nutrient is available, it may become toxic to plant growth. Utilization efficiencies of macronutrients directly correlate with yield and general plant tolerance, and increasing them will benefit the plants themselves and the environment by decreasing seepage to ground water.

Nitrogen is responsible for biosynthesis of amino and nucleic acids, prosthetic groups, plant hormones, plant chemical defenses, etc, and thus is utterly essential for the plant. For this reason, plants store nitrogen throughout their developmental stages, in the specific case of corn during the period of grain germination, mostly in the leaves and stalk. However, due to the low nitrogen use efficiency (NUE) of the main crops (e.g., in the range of only 30-70%), nitrogen supply needs to be replenished at least twice during the growing season. This requirement for fertilizer refill may become the rate-limiting element in plant growth and increase fertilizer expenses for the farmer. Limited land resources combined with rapid population growth will inevitably lead to added increase in fertilizer use. In light of this prediction, advanced, biotechnology-based solutions to allow stable high yields with an added potential to reduce fertilizer costs are highly desirable. Subsequently, developing plants with increased NUE will lower fertilizer input in crop cultivation, and allow growth on lower-quality soils.

The major agricultural crops (corn, rice, wheat, canola and soybean) account for over half of total human caloric intake, giving their yield and quality vast importance. They can be consumed either directly (eating their seeds which are also used as a source of sugars, oils and metabolites), or indirectly (eating meat products raised on processed seeds or forage). Various factors may influence a crop's yield, including but not limited to, quantity and size of the plant organs, plant architecture, vigor (e.g. seedling), growth rate, root development, utilization of water and nutrients (e.g., nitrogen), and stress tolerance. Plant yield may be amplified through multiple approaches; (1) enhancement of innate traits (e.g., dry matter accumulation rate, cellulose/lignin composition), (2) improvement of structural features (e.g., stalk strength, meristem size, plant branching pattern), and (3) amplification of seed yield and quality (e.g., fertilization efficiency, seed development, seed filling or content of oil, starch or protein). Increasing plant yield through any of the above methods would ultimately have many applications in agriculture and additional fields such as in the biotechnology industry.

Two main adverse environmental conditions, malnutrition (nutrient deficiency) and drought, elicit a response in the plant that mainly affects root architecture (Jiang and Huang (2001), Crop Sci 41:1168-1173; Lopez-Bucio et al. (2003), Curr Opin Plant Biol, 6:280-287; Morgan and Condon (1986), Aust J Plant Physiol 13:523-532), causing activation of plant metabolic pathways to maximize water assimilation. Improvement of root architecture, i.e. making branched and longer roots, allows the plant to reach water and nutrient/fertilizer deposits located deeper in the soil by an increase in soil coverage. Root morphogenesis has already shown to increase tolerance to low phosphorus availability in soybean (Miller et al., (2003), Funct Plant Biol 30:973-985) and maize (Zhu and Lynch (2004), Funct Plant Biol 31:949-958). Thus, genes governing enhancement of root architecture may be used to improve NUE and drought tolerance. An example for a gene associated with root developmental changes is ANR1, a putative transcription factor with a role in nitrate (NO3⁻) signaling. When expression of ANR1 is down-regulated, the resulting transgenic lines are defective in their root response to localized supplies of nitrate (Zhang and Forde (1998), Science 270:407). Enhanced root system and/or increased storage capabilities, which are seen in responses to different environmental stresses, are strongly favorable at normal or optimal growing conditions as well.

Abiotic stress refers to a range of suboptimal conditions as water deficit or drought, extreme temperatures and salt levels, and high or low light levels. High or low nutrient level also falls into the category of abiotic stress. The response to any stress may involve both stress specific and common stress pathways (Pastori and Foyer (2002), Plant Physiol, 129: 460-468), and drains energy from the plant, eventually resulting in lowered yield. Thus, distinguishing between the genes activated in each pathway and subsequent manipulation of only specific relevant genes could lead to a partial stress response without the parallel loss in yield. Contrary to the complex polygenic nature of plant traits responsible for adaptations to adverse environmental stresses, information on miRNAs involved in these responses is very limited. The most common approach for crop and horticultural improvements is through cross breeding, which is relatively slow, inefficient, and limited in the degree of variability achieved because it can only manipulate the naturally existing genetic diversity. Taken together with the limited genetic resources (i.e., compatible plant species) for crop improvement, conventional breeding is evidently unfavorable. By creating a pool of genetically modified plants, one broadens the possibilities for producing crops with improved economic or horticultural traits.

SUMMARY OF THE INVENTION

According to an aspect of some embodiments of the present invention there is provided a method of improving nitrogen use efficiency, abiotic stress tolerance, biomass, vigor or yield of a plant, the method comprising expressing within the plant an exogenous polynucleotide encoding a polypeptide having an amino acid sequence at least 80% homologous to the polypeptide selected from the group consisting of SEQ ID NOs: 687-981, 992-1248, 1281-1310, 1389-1391, and 2806-3081, wherein the polypeptide is capable of regulating nitrogen use efficiency of the plant, thereby improving nitrogen use efficiency, abiotic stress tolerance, biomass, vigor or yield of the plant.

According to an aspect of some embodiments of the present invention there is provided a transgenic plant exogenously expressing a polynucleotide encoding a polypeptide having an amino acid sequence at least 80% homologous to the polypeptide selected from the group consisting of SEQ ID NOs: 687-981, 992-1248, 1281-1310, 1389-1391, and 2806-3081, wherein the polypeptide is capable of regulating nitrogen use efficiency of the plant.

According to an aspect of some embodiments of the present invention there is provided a nucleic acid construct comprising a polynucleotide encoding a polypeptide having an amino acid sequence at least 80% homologous to SEQ ID NOs:687-981, 992-1248, 1281-1310, 1389-1391, and 2806-3081, wherein the polypeptide is capable of regulating nitrogen use efficiency of the plant, and wherein the polynucleotide is under a transcriptional control of a cis-acting regulatory element.

According to an aspect of some embodiments of the present invention there is provided a method of improving nitrogen use efficiency, abiotic stress tolerance, biomass, vigor or yield of a plant, the method comprising expressing within the plant an exogenous polynucleotide which downregulates an activity or expression of a polypeptide having an amino acid sequence at least 80% homologous to SEQ ID NOs: 311-514, 2007-2436, 1311-1320, 982-991, 1249-1280, 1321-1388, wherein the polypeptide is capable of regulating nitrogen use efficiency of the plant, thereby improving nitrogen use efficiency, abiotic stress tolerance, biomass, vigor or yield of the plant.

According to an aspect of some embodiments of the present invention there is provided a transgenic plant exogenously expressing a polynucleotide which downregulates an activity or expression of a polypeptide having an amino acid sequence at least 80% homologous to SEQ ID NOs: 311-514, 2007-2436, 1311-1320, 982-991, 1249-1280, 1321-1388, wherein the polypeptide is capable of regulating nitrogen use efficiency of the plant.

According to an aspect of some embodiments of the present invention there is provided a nucleic acid construct comprising a polynucleotide which downregulates an activity or expression of a polypeptide having an amino acid sequence at least 80% homologous to SEQ ID NOs: 311-514, 2007-2436, 1311-1320, 982-991, 1249-1280, 1321-1388, wherein the polypeptide is capable of regulating nitrogen use efficiency of a plant, the nucleic acid sequence being under the regulation of a cis-acting regulatory element.

According to some embodiments of the invention, the polynucleotide is selected from the group consisting of SEQ ID NO: 1392-1646, 1655-1888, 1911-1938, 3082-3316.

According to some embodiments of the invention, the polypeptide is selected from the group consisting of SEQ ID NO: 687-981, 992-1248, 1281-1310, 1389-1391, and 2806-3081.

According to some embodiments of the invention, the cis-acting regulatory element comprises a promoter.

According to some embodiments of the invention, the promoter comprises a tissue-specific promoter.

According to some embodiments of the invention, the tissue-specific promoter comprises a root specific promoter.

According to some embodiments of the invention, the method further comprising growing the plant under limiting nitrogen conditions.

According to some embodiments of the invention, the method further comprising growing the plant under abiotic stress.

According to some embodiments of the invention, the abiotic stress is selected from the group consisting of salinity, drought, water deprivation, flood, etiolation, low temperature, high temperature, heavy metal toxicity, anaerobiosis, nutrient deficiency, nutrient excess, atmospheric pollution and UV irradiation.

According to some embodiments of the invention, the plant of some embodiments of the invention being a monocotyledon.

According to some embodiments of the invention, the plant of some embodiments of the invention being a dicotyledon.

According to some embodiments of the invention, the polynucleotide acts by a mechanism selected from the group consisting of sense suppression, antisense suppression, ribozyme inhibition, and gene disruption.

Unless otherwise defined, all technical and/or scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which the invention pertains. Although methods and materials similar or equivalent to those described herein can be used in the practice or testing of embodiments of the invention, exemplary methods and/or materials are described below. In case of conflict, the patent specification, including definitions, will control. In addition, the materials, methods, and examples are illustrative only and are not intended to be necessarily limiting.

BRIEF DESCRIPTION OF THE DRAWINGS

Some embodiments of the invention are herein described, by way of example only, with reference to the accompanying drawings. With specific reference now to the drawings in detail, it is stressed that the particulars shown are by way of example and for purposes of illustrative discussion of embodiments of the invention. In this regard, the description taken with the drawings makes apparent to those skilled in the art how embodiments of the invention may be practiced.

In the drawings:

FIG. 1 is a scheme of a binary vector that can be used according to some embodiments of the invention.

DESCRIPTION OF SPECIFIC EMBODIMENTS OF THE INVENTION

The present invention, in some embodiments thereof, relates to nucleic acid agents for overexpressing or downregulating RNA interference targets and uses of same in improving nitrogen use efficiency, abiotic stress tolerance, biomass, vigor or yield of a plant.

Before explaining at least one embodiment of the invention in detail, it is to be understood that the invention is not necessarily limited in its application to the details set forth in the following description or exemplified by the Examples. The invention is capable of other embodiments or of being practiced or carried out in various ways.

The doubling of agricultural food production worldwide over the past four decades has been associated with a 7-fold increase in the use of nitrogen (N) fertilizers. As a consequence, both the recent and future intensification of the use of nitrogen fertilizers in agriculture already has and will continue to have major detrimental impacts on the diversity and functioning of the non-agricultural neighbouring bacterial, animal, and plant ecosystems. The most typical examples of such an impact are the eutrophication of freshwater and marine ecosystems as a result of leaching when high rates of nitrogen fertilizers are applied to agricultural fields. In addition, there can be gaseous emission of nitrogen oxides reacting with the stratospheric ozone and the emission of toxic ammonia into the atmosphere. Furthermore, farmers are facing increasing economic pressures with the rising fossil fuels costs required for production of nitrogen fertilizers.

It is therefore of major importance to identify the critical steps controlling plant nitrogen use efficiency (NUE). Such studies can be harnessed towards generating new energy crop species that have a larger capacity to produce biomass with the minimal amount of nitrogen fertilizer.

While reducing the present invention to practice, the present inventors have uncovered dsRNA sequences that are differentially expressed in maize plants grown under nitrogen limiting conditions versus corn plants grown under conditions wherein nitrogen is a non-limiting factor. Following further screening the present inventors were able to identify the target genes of these dsRNA sequences and suggest using same or sequences modulating expression of same in the generation of transgenic plants having improved nitrogen use efficiency.

According to some embodiments, the target genes or nucleic acid sequences controlling expression of same relay their effect by affecting at least one of:

root architecture so as to increase nutrient uptake;

activation of plant metabolic pathways so as to maximize nitrogen absorption or localization; or alternatively or additionally

modulating plant surface permeability.

Each of the above mechanisms may affect water uptake as well as salt absorption and therefore embodiments of the invention further relate to enhancement of abiotic stress tolerance, biomass, vigor or yield of the plant.

Thus, according to an aspect of the invention there is provided a method of improving nitrogen use efficiency, abiotic stress tolerance, biomass, vigor or yield of a plant, the method comprising expressing within the plant an exogenous polynucleotide encoding a polypeptide having an amino acid sequence at least 80% homologous to SEQ ID NOs: 687-981, 992-1248, 1281-1310, 1389-1391, and 2806-3081—wherein the polypeptide is capable of regulating nitrogen use efficiency of the plant, thereby improving nitrogen use efficiency, abiotic stress tolerance, biomass, vigor or yield of the plant.

As used herein the phrase “nitrogen use efficiency (NUE)” refers to a measure of crop production per unit of nitrogen fertilizer input. Fertilizer use efficiency (FUE) is a measure of NUE. Crop production can be measured by biomass, vigor or yield. The plant's nitrogen use efficiency is typically a result of an alteration in at least one of the uptake, spread, absorbance, accumulation, relocation (within the plant) and use of nitrogen absorbed by the plant. Improved NUE is with respect to that of a non-transgenic plant (i.e., lacking the transgene of the transgenic plant) of the same species and of the same developmental stage and grown under the same conditions.

As used herein the phrase “nitrogen-limiting conditions” refers to growth conditions which include a level (e.g., concentration) of nitrogen (e.g., ammonium or nitrate) applied, which is below the level needed for optimal plant metabolism, growth, reproduction and/or viability.

The phrase “abiotic stress” as used herein refers to any adverse effect on metabolism, growth, viability and/or reproduction of a plant. Abiotic stress can be induced by any of suboptimal environmental growth conditions such as, for example, water deficit or drought, flooding, freezing, low or high temperature, strong winds, heavy metal toxicity, anaerobiosis, high or low nutrient levels (e.g. nutrient deficiency), high or low salt levels (e.g. salinity), atmospheric pollution, high or low light intensities (e.g. insufficient light) or UV irradiation. Abiotic stress may be a short term effect (e.g. acute effect, e.g. lasting for about a week) or alternatively may be persistent (e.g. chronic effect, e.g. lasting for example 10 days or more). The present invention contemplates situations in which there is a single abiotic stress condition or alternatively situations in which two or more abiotic stresses occur.

According to an exemplary embodiment the abiotic stress refers to salinity.

According to another exemplary embodiment the abiotic stress refers to drought.

As used herein the phrase “abiotic stress tolerance” refers to the ability of a plant to endure an abiotic stress without exhibiting substantial physiological or physical damage (e.g. alteration in metabolism, growth, viability and/or reproductivity of the plant).

As used herein the term/phrase “biomass”, “biomass of a plant” or “plant biomass” refers to the amount (e.g., measured in grams of air-dry tissue) of a tissue produced from the plant in a growing season. An increase in plant biomass can be in the whole plant or in parts thereof such as aboveground (e.g. harvestable) parts, vegetative biomass, roots and/or seeds.

As used herein the term/phrase “vigor”, “vigor of a plant” or “plant vigor” refers to the amount (e.g., measured by weight) of tissue produced by the plant in a given time. Increased vigor could determine or affect the plant yield or the yield per growing time or growing area. In addition, early vigor (e.g. seed and/or seedling) results in improved field stand.

As used herein the term/phrase “yield”, “yield of a plant” or “plant yield” refers to the amount (e.g., as determined by weight or size) or quantity (e.g., numbers) of tissues or organs produced per plant or per growing season. Increased yield of a plant can affect the economic benefit one can obtain from the plant in a certain growing area and/or growing time.

According to an exemplary embodiment the yield is measured by cellulose content.

According to another exemplary embodiment the yield is measured by oil content.

According to another exemplary embodiment the yield is measured by protein content.

According to another exemplary embodiment, the yield is measured by seed number per plant or part thereof (e.g., kernel).

A plant yield can be affected by various parameters including, but not limited to, plant biomass; plant vigor; plant growth rate; seed yield; seed or grain quantity; seed or grain quality; oil yield; content of oil, starch and/or protein in harvested organs (e.g., seeds or vegetative parts of the plant); number of flowers (e.g. florets) per panicle (e.g. expressed as a ratio of number of filled seeds over number of primary panicles); harvest index; number of plants grown per area; number and size of harvested organs per plant and per area; number of plants per growing area (e.g. density); number of harvested organs in field; total leaf area; carbon assimilation and carbon partitioning (e.g. the distribution/allocation of carbon within the plant); resistance to shade; number of harvestable organs (e.g. seeds), seeds per pod, weight per seed; and modified architecture [such as increase stalk diameter, thickness or improvement of physical properties (e.g. elasticity)].

As used herein the term “improving” or “increasing” refers to at least about 2%, at least about 3%, at least about 4%, at least about 5%, at least about 10%, at least about 15%, at least about 20%, at least about 25%, at least about 30%, at least about 35%, at least about 40%, at least about 45%, at least about 50%, at least about 60%, at least about 70%, at least about 80%, at least about 90% or greater increase in NUE, in tolerance to abiotic stress, in yield, in biomass or in vigor of a plant, as compared to a native or wild-type plants [i.e., plants not genetically modified to express the biomolecules (polynucleotides or polypeptides) of the invention, e.g., a non-transformed plant of the same species and of the same developmental stage which is grown under the same growth conditions as the transformed plant].

Improved plant NUE is translated in the field into either harvesting similar quantities of yield, while implementing less fertilizers, or increased yields gained by implementing the same levels of fertilizers. Thus, improved NUE or FUE has a direct effect on plant yield in the field.

The term “plant” as used herein encompasses whole plants, ancestors and progeny of the plants and plant parts, including seeds, shoots, stems, roots (including tubers), and isolated plant cells, tissues and organs. The plant may be in any form including suspension cultures, embryos, meristematic regions, callus tissue, leaves, gametophytes, sporophytes, pollen, and microspores.

As used herein the phrase “plant cell” refers to plant cells which are derived and isolated from disintegrated plant cell tissue or plant cell cultures.

As used herein the phrase “plant cell culture” refers to any type of native (naturally occurring) plant cells, plant cell lines and genetically modified plant cells, which are not assembled to form a complete plant, such that at least one biological structure of a plant is not present. Optionally, the plant cell culture of this aspect of the present invention may comprise a particular type of a plant cell or a plurality of different types of plant cells. It should be noted that optionally plant cultures featuring a particular type of plant cell may be originally derived from a plurality of different types of such plant cells.

Any commercially or scientifically valuable plant is envisaged in accordance with these embodiments of the invention. Plants that are particularly useful in the methods of the invention include all plants which belong to the super family Viridiplantae, in particular monocotyledonous and dicotyledonous plants including a fodder or forage legume, ornamental plant, food crop, tree, or shrub selected from the list comprising Acacia spp., Acer spp., Actinidia spp., Aesculus spp., Agathis australis, Albizia amara, Alsophila tricolor, Andropogon spp., Arachis spp, Areca catechu, Astelia fragrans, Astragalus cicer, Baikiaea plurijuga, Betula spp., Brassica spp., Bruguiera gymnorrhiza, Burkea africana, Butea frondosa, Cadaba farinosa, Calliandra spp, Camellia sinensis, Canna indica, Capsicum spp., Cassia spp., Centroema pubescens, Chacoomeles spp., Cinnamomum cassia, Coffea arabica, Colophospermum mopane, Coronillia varia, Cotoneaster serotina, Crataegus spp., Cucumis spp., Cupressus spp., Cyathea dealbata, Cydonia oblonga, Cryptomeria japonica, Cymbopogon spp., Cynthea dealbata, Cydonia oblonga, Dalbergia monetaria, Davallia divaricata, Desmodium spp., Dicksonia squarosa, Dibeteropogon amplectens, Dioclea spp, Dolichos spp., Dorycnium rectum, Echinochloa pyramidalis, Ehraffia spp., Eleusine coracana, Eragrestis spp., Erythrina spp., Eucalyptus spp., Euclea schimperi, Eulalia vi/losa, Pagopyrum spp., Feijoa sellowlana, Fragaria spp., Flemingia spp, Freycinetia banksli, Geranium thunbergii, GinAgo biloba, Glycine javanica, Gliricidia spp, Gossypium hirsutum, Grevillea spp., Guibourtia coleosperma, Hedysarum spp., Hemaffhia altissima, Heteropogon contoffus, Hordeum vulgare, Hyparrhenia rufa, Hypericum erectum, Hypeffhelia dissolute, Indigo incamata, Iris spp., Leptarrhena pyrolifolia, Lespediza spp., Lettuca spp., Leucaena leucocephala, Loudetia simplex, Lotonus bainesli, Lotus spp., Macrotyloma axillare, Malus spp., Manihot esculenta, Medicago saliva, Metasequoia glyptostroboides, Musa sapientum, Nicotianum spp., Onobrychis spp., Ornithopus spp., Oryza spp., Peltophorum africanum, Pennisetum spp., Persea gratissima, Petunia spp., Phaseolus spp., Phoenix canariensis, Phormium cookianum, Photinia spp., Picea glauca, Pinus spp., Pisum sativam, Podocarpus totara, Pogonarthria fleckii, Pogonaffhria squarrosa, Populus spp., Prosopis cineraria, Pseudotsuga menziesii, Pterolobium stellatum, Pyrus communis, Quercus spp., Rhaphiolepsis umbellata, Rhopalostylis sapida, Rhus natalensis, Ribes grossularia, Ribes spp., Robinia pseudoacacia, Rosa spp., Rubus spp., Salix spp., Schyzachyrium sanguineum, Sciadopitys vefficillata, Sequoia sempervirens, Sequoiadendron giganteum, Sorghum bicolor, Spinacia spp., Sporobolus fimbriatus, Stiburus alopecuroides, Stylosanthos humilis, Tadehagi spp, Taxodium distichum, Themeda triandra, Trifolium spp., Triticum spp., Tsuga heterophylla, Vaccinium spp., Vicia spp., Vitis vinifera, Watsonia pyramidata, Zantedeschia aethiopica, Zea mays, amaranth, artichoke, asparagus, broccoli, Brussels sprouts, cabbage, canola, carrot, cauliflower, celery, collard greens, flax, kale, lentil, oilseed rape, okra, onion, potato, rice, soybean, straw, sugar beet, sugar cane, sunflower, tomato, squash tea, maize, wheat, barely, rye, oat, peanut, pea, lentil and alfalfa, cotton, rapeseed, canola, pepper, sunflower, tobacco, eggplant, eucalyptus, a tree, an ornamental plant, a perennial grass and a forage crop. Alternatively algae and other non-Viridiplantae can be used for the methods of the present invention.

According to some embodiments of the invention, the plant used by the method of the invention is a crop plant including, but not limited to, cotton, Brassica vegetables, oilseed rape, sesame, olive tree, palm oil, banana, wheat, corn or maize, barley, alfalfa, peanuts, sunflowers, rice, oats, sugarcane, soybean, turf grasses, barley, rye, sorghum, sugar cane, chicory, lettuce, tomato, zucchini, bell pepper, eggplant, cucumber, melon, watermelon, beans, hibiscus, okra, apple, rose, strawberry, chile, garlic, pea, lentil, canola, mums, arabidopsis, broccoli, cabbage, beet, quinoa, spinach, squash, onion, leek, tobacco, potato, sugarbeet, papaya, pineapple, mango, Arabidopsis thaliana, and also plants used in horticulture, floriculture or forestry, such as, but not limited to, poplar, fir, eucalyptus, pine, an ornamental plant, a perennial grass and a forage crop, coniferous plants, moss, algae, as well as other plants listed in World Wide Web (dot) nationmaster (dot) com/encyclopedia/Plantae.

According to a specific embodiment of the present invention, the plant comprises corn.

According to a specific embodiment of the present invention, the plant comprises sorghum.

As used herein, the phrase “exogenous polynucleotide” refers to a heterologous nucleic acid sequence or amino acid sequence which may not be naturally expressed within the plant or which overexpression in the plant is desired. The exogenous polynucleotide may be introduced into the plant in a stable or transient manner, so as to produce a ribonucleic acid (RNA) molecule or a polypeptide. It should be noted that the exogenous polynucleotide may comprise a nucleic acid sequence which is identical or partially homologous to an endogenous nucleic acid sequence of the plant.

A “transgenic plant” refers to a plant that has incorporated a nucleic acid sequence (i.e., polynucleotides encoding target genes or a silencing polynucleotide), including but not limited to genes that are not normally present in a host plant genome, nucleic acid sequences not normally transcribed into RNA, or any other genes or nucleic acid sequences that one desires to exogenously introduce into the wild-type plant, such as genes that normally may be present in the wild-type plant (control) but that one desires either to genetically engineer or to have altered expression.

Also contemplated are hybrids of the above described transgenic plants. A “hybrid plant” refers to a plant or a part thereof resulting from a cross between two parent plants, wherein one parent is a genetically engineered plant of the invention (transgenic plant expressing the polypeptides of the present invention). Such a cross can occur naturally by, for example, sexual reproduction, or artificially by, for example, in vitro nuclear fusion. Methods of plant breeding are well-known and within the level of one of ordinary skill in the art of plant biology.

The terms siRNA and miRNA are collectively referred to herein as RNA interfering molecules (RNAi).

As used herein, the phrase “siRNA” (also referred to herein interchangeably as “small interfering RNA” or “silencing RNA”, is a class of double-stranded RNA molecules, 20-25 nucleotides in length. The most notable role of siRNA is its involvement in the RNA interference (RNAi) pathway, where it interferes with the expression of a specific gene.

The siRNA precursor relates to a long dsRNA structure (at least 90% complementarity) of at least 30 bp.

As used herein, the phrase “microRNA (also referred to herein interchangeably as “miRNA” or “miR”) or a precursor thereof” refers to a microRNA (miRNA) molecule acting as a post-transcriptional regulator. Typically, the miRNA molecules are RNA molecules of about 20 to 22 nucleotides in length which can be loaded into a RISC complex and which direct the cleavage of another RNA molecule, wherein the other RNA molecule comprises a nucleotide sequence essentially complementary to the nucleotide sequence of the miRNA molecule.

Typically, a miRNA molecule is processed from a “pre-miRNA” or as used herein a precursor of a pre-miRNA molecule by proteins, such as DCL proteins, present in any plant cell and loaded onto a RISC complex where it can guide the cleavage of the target RNA molecules.

Pre-microRNA molecules are typically processed from pri-microRNA molecules (primary transcripts). The single stranded RNA segments flanking the pre-microRNA are important for processing of the pri-miRNA into the pre-miRNA. The cleavage site appears to be determined by the distance from the stem-ssRNA junction (Han et al. 2006, Cell 125, 887-901, 887-901).

As used herein, a “pre-miRNA” molecule is an RNA molecule of about 100 to about 200 nucleotides, preferably about 100 to about 130 nucleotides which can adopt a secondary structure comprising a double stranded RNA stem and a single stranded RNA loop (also referred to as “hairpin”) and further comprising the nucleotide sequence of the miRNA (and its complement sequence) in the double stranded RNA stem. According to a specific embodiment, the miRNA and its complement are located about 10 to about 20 nucleotides from the free ends of the miRNA double stranded RNA stem. The length and sequence of the single stranded loop region are not critical and may vary considerably, e.g. between 30 and 50 nt in length. The complementarity between the miRNA and its complement need not be perfect and about 1 to 3 bulges of unpaired nucleotides can be tolerated. The secondary structure adopted by an RNA molecule can be predicted by computer algorithms conventional in the art such as mFOLD. The particular strand of the double stranded RNA stem from the pre-miRNA which is released by DCL activity and loaded onto the RISC complex is determined by the degree of complementarity at the 5′ end, whereby the strand which at its 5′ end is the least involved in hydrogen bounding between the nucleotides of the different strands of the cleaved dsRNA stem is loaded onto the RISC complex and will determine the sequence specificity of the target RNA molecule degradation. However, if empirically the miRNA molecule from a particular synthetic pre-miRNA molecule is not functional (because the “wrong” strand is loaded on the RISC complex), it will be immediately evident that this problem can be solved by exchanging the position of the miRNA molecule and its complement on the respective strands of the dsRNA stem of the pre-miRNA molecule. As is known in the art, binding between A and U involving two hydrogen bounds, or G and U involving two hydrogen bounds is less strong that between G and C involving three hydrogen bounds. Exemplary siRNA sequences or precursors thereof as well as hairpin sequences and miRNA sequences are provided in Tables 1-4, below.

The tables provided in the Examples section are to be considered an integral part of the specification.

As used herein a “target gene” refers to a gene that is processed by microRNA activity. Typically the gene encodes a polypeptide which expression is downregulated due to microRNA processing.

Target genes are typically identified using the WMD3 website (http://wmd3dotweigelworlddotorg/).

As mentioned, the method of the present invention is effected by expressing within a plant an exogenous polynucleotide encoding a target gene of the RNA interfering molecules uncovered by the present inventors, as explained below.

As used herein, the phrase “expressing within the plant an exogenous polynucleotide” refers to upregulating the expression level of an exogenous polynucleotide within the plant e.g., by introducing the exogenous polynucleotide into a plant or plant cell and expressing by recombinant means, as described in detail hereinbelow.

As used herein “expressing” refers to expression at the mRNA level (e.g., in case the target gene expresses an mRNA product but no protein) or at the polypeptide level of the desired exogenous polynucleotide.

As used herein, the phrase “exogenous polynucleotide” refers to a heterologous nucleic acid sequence which may not be naturally expressed within the plant or which overexpression in the plant is desired (i.e., overexpression of an endogenous gene). The exogenous polynucleotide may be introduced into the plant in a stable or transient manner, so as to produce a ribonucleic acid (RNA) molecule and/or a polypeptide molecule. The exogenous polynucleotide may comprise a nucleic acid sequence which is identical or partially homologous to an endogenous nucleic acid sequence expressed within the plant.

The term “endogenous” as used herein refers to any polynucleotide or polypeptide which is present and/or naturally expressed within a plant or a cell thereof.

As used herein the term “polynucleotide” refers to a single or double stranded nucleic acid sequence which is isolated and provided in the form of an RNA sequence, a complementary polynucleotide sequence (cDNA), a genomic polynucleotide sequence (e.g. sequence isolated from a chromosome) and/or a composite polynucleotide sequences (e.g., a combination of the above). This term includes polynucleotides and/or oligonucleotides derived from naturally occurring nucleic acid molecules (e.g., RNA or DNA), synthetic polynucleotide and/or oligonucleotide molecules composed of naturally occurring bases, sugars, and covalent internucleoside linkages (e.g., backbone), as well as synthetic polynucleotides and/or oligonucleotides having non-naturally occurring portions, which function similarly to the respective naturally occurring portions.

The term “isolated” refers to at least partially separated from the natural environment e.g., from a plant cell.

Nucleic acid sequences of the polypeptides of some embodiments of the invention may be optimized for expression in a specific plant host. Examples of such sequence modifications include, but are not limited to, an altered G/C content to more closely approach that typically found in the plant species of interest, and the removal of codons atypically found in the plant species commonly referred to as codon optimization.

The phrase “codon optimization” refers to the selection of appropriate DNA nucleotides for use within a structural gene or fragment thereof that approaches codon usage within the plant of interest. Therefore, an optimized gene or nucleic acid sequence refers to a gene in which the nucleotide sequence of a native or naturally occurring gene has been modified in order to utilize statistically-preferred or statistically-favored codons within the plant. The nucleotide sequence typically is examined at the DNA level and the coding region optimized for expression in the plant species determined using any suitable procedure, for example as described in Sardana et al. (1996, Plant Cell Reports 15:677-681). In this method, the standard deviation of codon usage, a measure of codon usage bias, may be calculated by first finding the squared proportional deviation of usage of each codon of the native gene relative to that of highly expressed plant genes, followed by a calculation of the average squared deviation. The formula used is: 1 SDCU=n=1 N [(Xn−Yn)/Yn]2/N, where Xn refers to the frequency of usage of codon n in highly expressed plant genes, where Yn to the frequency of usage of codon n in the gene of interest and N refers to the total number of codons in the gene of interest. A table of codon usage from highly expressed genes of dicotyledonous plants is compiled using the data of Murray et al. (1989, Nuc Acids Res. 17:477-498).

One method of optimizing the nucleic acid sequence in accordance with the preferred codon usage for a particular plant cell type is based on the direct use, without performing any extra statistical calculations, of codon optimization tables such as those provided on-line at the Codon Usage Database through the NIAS (National Institute of Agrobiological Sciences) DNA bank in Japan (wwwdotkazusadotordotjp/codon/). The Codon Usage Database contains codon usage tables for a number of different species, with each codon usage table having been statistically determined based on the data present in Genbank.

By using the above tables to determine the most preferred or most favored codons for each amino acid in a particular species (for example, rice), a naturally-occurring nucleotide sequence encoding a protein of interest can be codon optimized for that particular plant species. This is effected by replacing codons that may have a low statistical incidence in the particular species genome with corresponding codons, in regard to an amino acid, that are statistically more favored. However, one or more less-favored codons may be selected to delete existing restriction sites, to create new ones at potentially useful junctions (5′ and 3′ ends to add signal peptide or termination cassettes, internal sites that might be used to cut and splice segments together to produce a correct full-length sequence), or to eliminate nucleotide sequences that may negatively effect mRNA stability or expression.

The naturally-occurring encoding nucleotide sequence may already, in advance of any modification, contain a number of codons that correspond to a statistically-favored codon in a particular plant species. Therefore, codon optimization of the native nucleotide sequence may comprise determining which codons, within the native nucleotide sequence, are not statistically-favored with regards to a particular plant, and modifying these codons in accordance with a codon usage table of the particular plant to produce a codon optimized derivative. A modified nucleotide sequence may be fully or partially optimized for plant codon usage provided that the protein encoded by the modified nucleotide sequence is produced at a level higher than the protein encoded by the corresponding naturally occurring or native gene. Construction of synthetic genes by altering the codon usage is described in for example PCT Patent Application 93/07278.

Target genes which are contemplated according to the present teachings are provided in the polynucleotide sequences which comprise nucleic acid sequences as set forth in SEQ ID NO: 1392-1646, 1655-1888, 1911-1938, 3082-3316. However the present teachings also relate to orthologs or homologs at least about 60%, at least about 65%, at least about 70%, at least about 75%, at least about 80%, at least about 85%, at least about 90%, or at least about 95% or more identical or similar to SEQ ID NO: 1392-1646, 1655-1888, 1911-1938, 3082-3316. Parameters for determining the level of identity are provided hereinbelow.

Alternatively or additionally, target genes which are contemplated according to the present teachings are provided in the polypeptide sequences which comprise amino acid sequences as set forth in SEQ ID NO: 687-981, 992-1248, 1281-1310, 1389-1391, and 2806-3081 (Tables 6 and 8). However the present teachings also relate to of orthologs or homologs at least about 60%, at least about 65%, at least about 70%, at least about 75%, at least about 80%, at least about 85%, at least about 90%, or at least about 95% or more identical or similar to SEQ ID NO: 687-981, 992-1248, 1281-1310, 1389-1391, and 2806-3081 (Tables 6 and 8).

Homology (e.g., percent homology, identity+similarity) can be determined using any homology comparison software, including for example, the TBLASTN software of the National Center of Biotechnology Information (NCBI) such as by using default parameters, when starting from a polypeptide sequence; or the tBLASTX algorithm (available via the NCBI) such as by using default parameters, which compares the six-frame conceptual translation products of a nucleotide query sequence (both strands) against a protein sequence database.

According to some embodiments of the invention, the term “homology” or “homologous” refers to identity of two or more nucleic acid sequences; or identity of two or more amino acid sequences.

Homologous sequences include both orthologous and paralogous sequences. The term “paralogous” relates to gene-duplications within the genome of a species leading to paralogous genes. The term “orthologous” relates to homologous genes in different organisms due to ancestral relationship.

One option to identify orthologues in monocot plant species is by performing a reciprocal blast search. This may be done by a first blast involving blasting the sequence-of-interest against any sequence database, such as the publicly available NCBI database which may be found at: Hypertext Transfer Protocol://World Wide Web (dot) ncbi (dot) nlm (dot) nih (dot) gov. The blast results may be filtered. The full-length sequences of either the filtered results or the non-filtered results are then blasted back (second blast) against the sequences of the organism from which the sequence-of-interest is derived. The results of the first and second blasts are then compared. An orthologue is identified when the sequence resulting in the highest score (best hit) in the first blast identifies in the second blast the query sequence (the original sequence-of-interest) as the best hit. Using the same rational a paralogue (homolog to a gene in the same organism) is found. In case of large sequence families, the ClustalW program may be used [Hypertext Transfer Protocol://World Wide Web (dot) ebi (dot) ac (dot) uk/Tools/clustalw2/index (dot) html], followed by a neighbor-joining tree (Hypertext Transfer Protocol://en (dot) wikipedia (dot) org/wiki/Neighbor-joining) which helps visualizing the clustering.

As mentioned the present inventors have also identified genes which down-regulation thereof may be done in order to improve their NUE, biomass, vigor, yield and abiotic stress tolerance.

Thus, according to an aspect of the invention there is provided a method of improving nitrogen use efficiency, abiotic stress tolerance, biomass, vigor or yield of a plant, the method comprising expressing within the plant an exogenous polynucleotide which downregulates an activity or expression of a polypeptide having an amino acid sequence at least 80%, 85%, 90%, 95%, or 100% homologous or identical to SEQ ID NOs: 311-514, 2007-2436, 1311-1320, 982-991, 1249-1280, 1321-1388 (Tables 5, 7 and 9), wherein the polypeptide is capable of regulating nitrogen use efficiency of the plant, thereby improving nitrogen use efficiency, abiotic stress tolerance, biomass, vigor or yield of the plant.

Down regulation of activity or expression is by at least 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90% or even complete (100%) loss of activity or expression. Assays for measuring gene expression can be effected at the protein level (e.g., Western blot, ELISA) or at the mRNA level such as by RT-PCR.

According to a specific embodiment the amino acid sequence of the target gene is as set forth in SEQ ID NOs: 311-514, 2007-2436, 1311-1320, 982-991, 1249-1280, 1321-1388 of Tables 5, 7 and 9.

Alternatively or additionally, the amino acid sequence of the target gene is encoded by a polynucleotide sequence as set forth in SEQ ID NOs: 515-686, 2437-2805, 1939-1948, 1647-1654, 1889-1910, 1949-2004 of Tables 5, 7 and 9.

Examples of polynucleotide downregulating agents that inhibit (also referred to herein as inhibitors or nucleic acid agents) the expression of a target gene are given below.

1. Polynucleotide-Based Inhibition of Gene Expression.

It will be appreciated, that any of these methods when specifically referring to downregulating expression/activity of the target genes can be used, at least in part, to downregulate expression or activity of endogenous RNA molecules.

i. Sense Suppression/Cosuppression

In some embodiments of the invention, inhibition of the expression of target gene may be obtained by sense suppression or cosuppression. For cosuppression, an expression cassette is designed to express an RNA molecule corresponding to all or part of a messenger RNA encoding a target gene in the “sense” orientation. Over-expression of the RNA molecule can result in reduced expression of the native gene. Accordingly, multiple plant lines transformed with the cosuppression expression cassette are screened to identify those that show the greatest inhibition of target gene expression.

The polynucleotide used for cosuppression may correspond to all or part of the sequence encoding the target gene, all or part of the 5′ and/or 3′ untranslated region of a target transcript, or all or part of both the coding sequence and the untranslated regions of a transcript encoding the target gene. In some embodiments where the polynucleotide comprises all or part of the coding region for the target gene, the expression cassette is designed to eliminate the start codon of the polynucleotide so that no protein product will be transcribed.

Cosuppression may be used to inhibit the expression of plant genes to produce plants having undetectable protein levels for the proteins encoded by these genes. See, for example, Broin, et al., (2002) Plant Cell 15:1517-1532. Cosuppression may also be used to inhibit the expression of multiple proteins in the same plant. Methods for using cosuppression to inhibit the expression of endogenous genes in plants are described in Flavell, et al., (1995) Proc. Natl. Acad. Sci. USA 91:3590-3596; Jorgensen, et al., (1996) Plant Mol. Biol. 31:957-973; Johansen and Carrington, (2001) Plant Physiol. 126:930-938; Broin, et al., (2002) Plant Cell 15:1517-1532; Stoutjesdijk, et al., (2002) Plant Physiol. 129:1723-1731; Yu, et al., (2003) Phytochemistry 63:753-763; and U.S. Pat. Nos. 5,035,323, 5,283,185 and 5,952,657; each of which is herein incorporated by reference. The efficiency of cosuppression may be increased by including a poly-dt region in the expression cassette at a position 3′ to the sense sequence and 5′ of the polyadenylation signal. See, US Patent Publication Number 20020058815, herein incorporated by reference. Typically, such a nucleotide sequence has substantial sequence identity to the sequence of the transcript of the endogenous gene, optimally greater than about 65% sequence identity, more optimally greater than about 85% sequence identity, most optimally greater than about 95% sequence identity. See, U.S. Pat. Nos. 5,283,185 and 5,035,323; herein incorporated by reference.

Transcriptional gene silencing (TGS) may be accomplished through use of hpRNA constructs wherein the inverted repeat of the hairpin shares sequence identity with the promoter region of a gene to be silenced. Processing of the hpRNA into short RNAs which can interact with the homologous promoter region may trigger degradation or methylation to result in silencing. (Aufsatz, et al., (2002) PNAS 99(4):16499-16506; Mette, et al., (2000) EMBO J. 19(19):5194-5201),

ii. Antisense Suppression

In some embodiments of the invention, inhibition of the expression of the target gene may be obtained by antisense suppression. For antisense suppression, the expression cassette is designed to express an RNA molecule complementary to all or part of a messenger RNA encoding the target gene. Over-expression of the antisense RNA molecule can result in reduced expression of the native gene. Accordingly, multiple plant lines transformed with the antisense suppression expression cassette are screened to identify those that show the greatest inhibition of target gene expression.

The polynucleotide for use in antisense suppression may correspond to all or part of the complement of the sequence encoding the target gene, all or part of the complement of the 5′ and/or 3′ untranslated region of the target gene transcript, or all or part of the complement of both the coding sequence and the untranslated regions of a transcript encoding the target gene. In addition, the antisense polynucleotide may be fully complementary (i.e., 100% identical to the complement of the target sequence) or partially complementary (i.e., less than 100% identical to the complement of the target sequence) to the target sequence. Antisense suppression may be used to inhibit the expression of multiple proteins in the same plant. Furthermore, portions of the antisense nucleotides may be used to disrupt the expression of the target gene. Generally, sequences of at least 50 nucleotides, 100 nucleotides, 200 nucleotides, 300, 500, 550, 500, 550 or greater may be used. Methods for using antisense suppression to inhibit the expression of endogenous genes in plants are described, for example, in Liu, et al., (2002) Plant Physiol. 129:1732-1753 and U.S. Pat. No. 5,759,829, which is herein incorporated by reference. Efficiency of antisense suppression may be increased by including a poly-dt region in the expression cassette at a position 3′ to the antisense sequence and 5′ of the polyadenylation signal. See, US Patent Publication Number 20020058815.

iii. Double-Stranded RNA Interference

In some embodiments of the invention, inhibition of the expression of a target gene may be obtained by double-stranded RNA (dsRNA) interference. For dsRNA interference, a sense RNA molecule like that described above for cosuppression and an antisense RNA molecule that is fully or partially complementary to the sense RNA molecule are expressed in the same cell, resulting in inhibition of the expression of the corresponding endogenous messenger RNA.

Expression of the sense and antisense molecules can be accomplished by designing the expression cassette to comprise both a sense sequence and an antisense sequence. Alternatively, separate expression cassettes may be used for the sense and antisense sequences. Multiple plant lines transformed with the dsRNA interference expression cassette or expression cassettes are then screened to identify plant lines that show the greatest inhibition of target gene expression. Methods for using dsRNA interference to inhibit the expression of endogenous plant genes are described in Waterhouse, et al., (1998) Proc. Natl. Acad. Sci. USA 95:13959-13965, Liu, et al., (2002) Plant Physiol. 129:1732-1753, and WO 99/59029, WO 99/53050, WO 99/61631, and WO 00/59035,

iv. Hairpin RNA Interference and Intron-Containing Hairpin RNA Interference

In some embodiments of the invention, inhibition of the expression of one or more target gene may be obtained by hairpin RNA (hpRNA) interference or intron-containing hairpin RNA (ihpRNA) interference. These methods are highly efficient at downregulating the expression of endogenous genes. See, Waterhouse and Helliwell, (2003) Nat. Rev. Genet. 5:29-38 and the references cited therein.

For hpRNA interference, the expression cassette is designed to express an RNA molecule that hybridizes with itself to form a hairpin structure that comprises a single-stranded loop region and a base-paired stem. The base-paired stem region comprises a sense sequence corresponding to all or part of the endogenous messenger RNA encoding the gene whose expression is to be inhibited, and an antisense sequence that is fully or partially complementary to the sense sequence. Thus, the base-paired stem region of the molecule generally determines the specificity of the RNA interference. hpRNA molecules are highly efficient at inhibiting the expression of endogenous genes, and the RNA interference they induce is inherited by subsequent generations of plants. See, for example, Chuang and Meyerowitz, (2000) Proc. Natl. Acad. Sci. USA 97:5985-5990; Stoutjesdijk, et al., (2002) Plant Physiol. 129:1723-1731; and Waterhouse and Helliwell, (2003) Nat. Rev. Genet. 5:29-38. Methods for using hpRNA interference to inhibit or silence the expression of genes are described, for example, in Chuang and Meyerowitz, (2000) Proc. Natl. Acad. Sci. USA 97:5985-5990; Stoutjesdijk, et al., (2002) Plant Physiol. 129:1723-1731; Waterhouse and Helliwell, (2003) Nat. Rev. Genet. 5:29-38; Pandolfini, et al., BMC Biotechnology 3:7, and US Patent Publication Number 20030175965; each of which is herein incorporated by reference. A transient assay for the efficiency of hpRNA constructs to silence gene expression in vivo has been described by Panstruga, et al., (2003) Mol. Biol. Rep. 30:135-150, herein incorporated by reference.

ForihpRNA, the interfering molecules have the same general structure as for hpRNA, but the RNA molecule additionally comprises an intron that is capable of being spliced in the cell in which the ihpRNA is expressed. The use of an intron minimizes the size of the loop in the hairpin RNA molecule following splicing, and this increases the efficiency of interference. See, for example, Smith, et al., (2000) Nature 507:319-320. In fact, Smith, et al., show 100% suppression of endogenous gene expression using ihpRNA-mediated interference. Methods for using ihpRNA interference to inhibit the expression of endogenous plant genes are described, for example, in Smith, et al., (2000) Nature 507:319-320; Wesley, et al., (2001) Plant J. 27:581-590; Wang and Waterhouse, (2001) Curr. Opin. Plant Biol. 5:156-150; Waterhouse and Helliwell, (2003) Nat. Rev. Genet. 5:29-38; Helliwell and Waterhouse, (2003) Methods 30:289-295, and US Patent Publication Number 20030180955, each of which is herein incorporated by reference.

The expression cassette for hpRNA interference may also be designed such that the sense sequence and the antisense sequence do not correspond to an endogenous RNA. In this embodiment, the sense and antisense sequence flank a loop sequence that comprises a nucleotide sequence corresponding to all or part of the endogenous messenger RNA of the target gene. Thus, it is the loop region that determines the specificity of the RNA interference. See, for example, WO 02/00905, herein incorporated by reference.

v. Amplicon-Mediated Interference

Amplicon expression cassettes comprise a plant virus-derived sequence that contains all or part of the target gene but generally not all of the genes of the native virus. The viral sequences present in the transcription product of the expression cassette allow the transcription product to direct its own replication. The transcripts produced by the amplicon may be either sense or antisense relative to the target sequence (i.e., the messenger RNA for target gene). Methods of using amplicons to inhibit the expression of endogenous plant genes are described, for example, in Angell and Baulcombe, (1997) EMBO J. 16:3675-3685, Angell and Baulcombe, (1999) Plant J. 20:357-362, and U.S. Pat. No. 6,656,805, each of which is herein incorporated by reference.

vi. Ribozymes

In some embodiments, the polynucleotide expressed by the expression cassette of the invention is catalytic RNA or has ribozyme activity specific for the messenger RNA of target gene. Thus, the polynucleotide causes the degradation of the endogenous messenger RNA, resulting in reduced expression of the target gene. This method is described, for example, in U.S. Pat. No. 5,987,071, herein incorporated by reference.

2. Gene Disruption

In some embodiments of the present invention, the activity of a miRNA or a target gene is reduced or eliminated by disrupting the gene encoding the target polypeptide. The gene encoding the target polypeptide may be disrupted by any method known in the art. For example, in one embodiment, the gene is disrupted by transposon tagging. In another embodiment, the gene is disrupted by mutagenizing plants using random or targeted mutagenesis, and selecting for plants that have reduced response regulator activity.

Any of the nucleic acid agents described herein (for overexpression or downregulation) can be provided to the plant as naked RNA or expressed from a nucleic acid expression construct, where it is operaly linked to a regulatory sequence.

According to a specific embodiment of the invention, there is provided a nucleic acid construct comprising a nucleic acid sequence (a polynucleotide) encoding the RNAi target polypeptide or the nucleic acid agent for downregulating the expression of the target gene, the nucleic acid sequence being under a transcriptional control a cis-acting regulatory element.

Exemplary nucleic acid constructs which can be used for plant transformation include, but are not limited to, pORE156, pORE164, pORE167 and pORE169, which are all constructed by ligating the appropriate DNA fragments into the pORE E2 binary vector (Accession number: AY562535, FIG. 1) under the transcriptional control of a promoter.

A coding nucleic acid sequence is “operably linked” or “transcriptionally linked to a regulatory sequence (e.g., promoter)” if the regulatory sequence is capable of exerting a regulatory effect on the coding sequence linked thereto. Thus the regulatory sequence controls the transcription of the target polynucleotide.

The term “regulatory sequence”, as used herein, means any DNA, that is involved in driving transcription and controlling (i.e., regulating) the timing and level of transcription of a given DNA sequence, such as a DNA coding for the target polypeptide, as described above. For example, a 5′ regulatory region (or “promoter region”) is a DNA sequence located upstream (i.e., 5′) of a coding sequence and which comprises the promoter and the 5′-untranslated leader sequence. A 3′ regulatory region is a DNA sequence located downstream (i.e., 3′) of the coding sequence and which comprises suitable transcription termination (and/or regulation) signals, including one or more polyadenylation signals.

For the purpose of the invention, the promoter is a plant-expressible promoter. As used herein, the term “plant-expressible promoter” means a DNA sequence which is capable of controlling (initiating) transcription in a plant cell. This includes any promoter of plant origin, but also any promoter of non-plant origin which is capable of directing transcription in a plant cell, i.e., certain promoters of viral or bacterial origin Thus, any suitable promoter sequence can be used by the nucleic acid construct of the present invention. According to some embodiments of the invention, the promoter is a constitutive promoter, a tissue-specific promoter or an inducible promoter (e.g. an abiotic stress-inducible promoter).

Suitable constitutive promoters include, for example, hydroperoxide lyase (HPL) promoter, CaMV 35S promoter (Odell et al, Nature 313:810-812, 1985); Arabidopsis At6669 promoter (see PCT Publication No. WO04081173A2); Arabidopsis new At6669 promoter; maize Ubi 1 (Christensen et al., Plant Sol. Biol. 18:675-689, 1992); rice actin (McElroy et al., Plant Cell 2:163-171, 1990); pEMU (Last et al, Theor. Appl. Genet. 81:581-588, 1991); CaMV 19S (Nilsson et al, Physiol. Plant 100:456-462, 1997); GOS2 (de Pater et al, Plant J November; 2(6):837-44, 1992); ubiquitin (Christensen et al, Plant MoI. Biol. 18: 675-689, 1992); Rice cyclophilin (Bucholz et al, Plant MoI Biol. 25(5):837-43, 1994); Maize H3 histone (Lepetit et al, MoI. Gen. Genet. 231: 276-285, 1992); Actin 2 (An et al, Plant J. 10(1);107-121, 1996) and Synthetic Super MAS (Ni et al., The Plant Journal 7: 661-76, 1995). Other constitutive promoters include those in U.S. Pat. Nos. 5,659,026, 5,608,149; 5,608,144; 5,604,121; 5,569,597; 5,466,785; 5,399,680; 5,268,463; and 5,608,142.

Suitable tissue-specific promoters include, but not limited to, leaf-specific promoters [such as described, for example, by Yamamoto et al., Plant J. 12:255-265, 1997; Kwon et al., Plant Physiol. 105:357-67, 1994; Yamamoto et al., Plant Cell Physiol. 35:773-778, 1994; Gotor et al., Plant J. 3:509-18, 1993; Orozco et al., Plant MoI. Biol. 23:1129-1138, 1993; and Matsuoka et al., Proc. Natl. Acad. Sci. USA 90:9586-9590, 1993], seed-preferred promoters [e.g., from seed specific genes (Simon, et al., Plant MoI. Biol. 5. 191, 1985; Scofield, et al., J. Biol. Chem. 262: 12202, 1987; Baszczynski, et al., Plant MoI. Biol. 14: 633, 1990), Brazil Nut albumin (Pearson′ et al., Plant MoI. Biol. 18: 235-245, 1992), legumin (Ellis, et al. Plant MoI. Biol. 10: 203-214, 1988), Glutelin (rice) (Takaiwa, et al., MoI. Gen. Genet. 208: 15-22, 1986; Takaiwa, et al., FEBS Letts. 221: 43-47, 1987), Zein (Matzke et al., Plant MoI Biol, 143)323-32 1990), napA (Stalberg, et al., Planta 199: 515-519, 1996), Wheat SPA (Albani et al, Plant Cell, 9: 171-184, 1997), sunflower oleosin (Cummins, et al, Plant MoI. Biol. 19: 873-876, 1992)], endosperm specific promoters [e.g., wheat LMW and HMW, glutenin-1 (MoI Gen Genet 216:81-90, 1989; NAR 17:461-2), wheat a, b and g gliadins (EMBO3: 1409-15, 1984), Barley ltrl promoter, barley Bl, C, D hordein (Theor Appl Gen 98:1253-62, 1999; Plant J 4:343-55, 1993; MoI Gen Genet 250:750-60, 1996), Barley DOF (Mena et al., The Plant Journal, 116(1): 53-62, 1998), Biz2 (EP99106056.7), Synthetic promoter (Vicente-Carbajosa et al., Plant J. 13: 629-640, 1998), rice prolamin NRP33, rice-globulin GIb-I (Wu et al., Plant Cell Physiology 39(8) 885-889, 1998), rice alpha-globulin REB/OHP-1 (Nakase et al. Plant MoI. Biol. 33: 513-S22, 1997), rice ADP-glucose PP (Trans Res 6:157-68, 1997), maize ESR gene family (Plant J 12:235-46, 1997), sorghum gamma-kafirin (PMB 32:1029-35, 1996); e.g., the Napin promoter], embryo specific promoters [e.g., rice OSH1 (Sato et al, Proc. Natl. Acad. Sci. USA, 93: 8117-8122), KNOX (Postma-Haarsma et al, Plant MoI. Biol. 39:257-71, 1999), rice oleosin (Wu et at, J. Biochem., 123:386, 1998)], and flower-specific promoters [e.g., AtPRP4, chalene synthase (chsA) (Van der Meer, et al., Plant MoI. Biol. 15, 95-109, 1990), LAT52 (Twell et al., MoI. Gen Genet. 217:240-245; 1989), apetala-3]. Also contemplated are root-specific promoters such as the ROOTP promoter described in Vissenberg K, et al. Plant Cell Physiol. 2005 January; 46(1):192-200.

The nucleic acid construct of some embodiments of the invention can further include an appropriate selectable marker and/or an origin of replication.

The nucleic acid construct of some embodiments of the invention can be utilized to stably or transiently transform plant cells. In stable transformation, the exogenous polynucleotide is integrated into the plant genome and as such it represents a stable and inherited trait. In transient transformation, the exogenous polynucleotide is expressed by the cell transformed but it is not integrated into the genome and as such it represents a transient trait.

There are various methods of introducing foreign genes into both monocotyledonous and dicotyledonous plants (Potrykus, L, Annu. Rev. Plant. Physiol, Plant. MoI. Biol. (1991) 42:205-225; Shimamoto et al., Nature (1989) 338:274-276).

The principle methods of causing stable integration of exogenous DNA into plant genomic DNA include two main approaches:

(i) Agrobacterium-mediated gene transfer (e.g., T-DNA using Agrobacterium tumefaciens or Agrobacterium rhizogenes); see for example, Klee et al. (1987) Annu. Rev. Plant Physiol. 38:467-486; Klee and Rogers in Cell Culture and Somatic Cell Genetics of Plants, Vol. 6, Molecular Biology of Plant Nuclear Genes, eds. Schell, J., and Vasil, L. K., Academic Publishers, San Diego, Calif. (1989) p. 2-25; Gatenby, in Plant Biotechnology, eds. Kung, S, and Arntzen, C. J., Butterworth Publishers, Boston, Mass. (1989) p. 93-112.

(ii) Direct DNA uptake: Paszkowski et al., in Cell Culture and Somatic Cell Genetics of Plants, Vol. 6, Molecular Biology of Plant Nuclear Genes eds. Schell, J., and Vasil, L. K., Academic Publishers, San Diego, Calif. (1989) p. 52-68; including methods for direct uptake of DNA into protoplasts, Toriyama, K. et al. (1988) Bio/Technology 6:1072-1074. DNA uptake induced by brief electric shock of plant cells: Zhang et al. Plant Cell Rep. (1988) 7:379-384. Fromm et al. Nature (1986) 319:791-793. DNA injection into plant cells or tissues by particle bombardment, Klein et al. Bio/Technology (1988) 6:559-563; McCabe et al. Bio/Technology (1988) 6:923-926; Sanford, Physiol. Plant. (1990) 79:206-209; by the use of micropipette systems: Neuhaus et al., Theor. Appl. Genet. (1987) 75:30-36; Neuhaus and Spangenberg, Physiol. Plant. (1990) 79:213-217; glass fibers or silicon carbide whisker transformation of cell cultures, embryos or callus tissue, U.S. Pat. No. 5,464,765 or by the direct incubation of DNA with germinating pollen, DeWet et al. in Experimental Manipulation of Ovule Tissue, eds. Chapman, G. P. and Mantell, S. H. and Daniels, W. Longman, London, (1985) p. 197-209; and Ohta, Proc. Natl. Acad. Sci. USA (1986) 83:715-719.

The Agrobacterium system includes the use of plasmid vectors that contain defined DNA segments that integrate into the plant genomic DNA. Methods of inoculation of the plant tissue vary depending upon the plant species and the Agrobacterium delivery system. A widely used approach is the leaf disc procedure which can be performed with any tissue explant that provides a good source for initiation of whole plant differentiation. See, e.g., Horsch et al. in Plant Molecular Biology Manual A5, Kluwer Academic Publishers, Dordrecht (1988) p. 1-9. A supplementary approach employs the Agrobacterium delivery system in combination with vacuum infiltration. The Agrobacterium system is especially viable in the creation of transgenic dicotyledonous plants.

According to a specific embodiment of the present invention, the exogenous polynucleotide is introduced into the plant by infecting the plant with a bacteria, such as using a floral dip transformation method (as described in further detail in Example 5, of the Examples section which follows).

There are various methods of direct DNA transfer into plant cells. In electroporation, the protoplasts are briefly exposed to a strong electric field. In microinjection, the DNA is mechanically injected directly into the cells using very small micropipettes. In microparticle bombardment, the DNA is adsorbed on microprojectiles such as magnesium sulfate crystals or tungsten particles, and the microprojectiles are physically accelerated into cells or plant tissues.

Following stable transformation plant propagation is exercised. The most common method of plant propagation is by seed. Regeneration by seed propagation, however, has the deficiency that due to heterozygosity there is a lack of uniformity in the crop, since seeds are produced by plants according to the genetic variances governed by Mendelian rules. Basically, each seed is genetically different and each will grow with its own specific traits. Therefore, it is preferred that the transformed plant be produced such that the regenerated plant has the identical traits and characteristics of the parent transgenic plant. For this reason it is preferred that the transformed plant be regenerated by micropropagation which provides a rapid, consistent reproduction of the transformed plants.

Micropropagation is a process of growing new generation plants from a single piece of tissue that has been excised from a selected parent plant or cultivar. This process permits the mass reproduction of plants having the preferred tissue expressing the fusion protein. The new generation plants which are produced are genetically identical to, and have all of the characteristics of, the original plant. Micropropagation allows mass production of quality plant material in a short period of time and offers a rapid multiplication of selected cultivars in the preservation of the characteristics of the original transgenic or transformed plant. The advantages of cloning plants are the speed of plant multiplication and the quality and uniformity of plants produced.

Micropropagation is a multi-stage procedure that requires alteration of culture medium or growth conditions between stages. Thus, the micropropagation process involves four basic stages: Stage one, initial tissue culturing; stage two, tissue culture multiplication; stage three, differentiation and plant formation; and stage four, greenhouse culturing and hardening. During stage one, initial tissue culturing, the tissue culture is established and certified contaminant-free. During stage two, the initial tissue culture is multiplied until a sufficient number of tissue samples are produced to meet production goals. During stage three, the tissue samples grown in stage two are divided and grown into individual plantlets. At stage four, the transformed plantlets are transferred to a greenhouse for hardening where the plants' tolerance to light is gradually increased so that it can be grown in the natural environment.

Although stable transformation is presently preferred, transient transformation of leaf cells, meristematic cells or the whole plant is also envisaged by the present invention.

Transient transformation can be effected by any of the direct DNA transfer methods described above or by viral infection using modified plant viruses.

Viruses that have been shown to be useful for the transformation of plant hosts include CaMV, Tobacco mosaic virus (TMV), brome mosaic virus (BMV) and Bean Common Mosaic Virus (BV or BCMV). Transformation of plants using plant viruses is described in U.S. Pat. No. 4,855,237 (bean golden mosaic virus; BGV), EP-A 67,553 (TMV), Japanese Published Application No. 63-14693 (TMV), EPA 194,809 (BV), EPA 278,667 (BV); and Gluzman, Y. et al., Communications in Molecular Biology: Viral Vectors, Cold Spring Harbor Laboratory, New York, pp. 172-189 (1988). Pseudovirus particles for use in expressing foreign DNA in many hosts, including plants are described in WO 87/06261. According to some embodiments of the invention, the virus used for transient transformations is avirulent and thus is incapable of causing severe symptoms such as reduced growth rate, mosaic, ring spots, leaf roll, yellowing, streaking, pox formation, tumor formation and pitting. A suitable avirulent virus may be a naturally occurring avirulent virus or an artificially attenuated virus. Virus attenuation may be effected by using methods well known in the art including, but not limited to, sub-lethal heating, chemical treatment or by directed mutagenesis techniques such as described, for example, by Kurihara and Watanabe (Molecular Plant Pathology 4:259-269, 2003), Galon et al. (1992), Atreya et al. (1992) and Huet et al. (1994).

Suitable virus strains can be obtained from available sources such as, for example, the American Type culture Collection (ATCC) or by isolation from infected plants. Isolation of viruses from infected plant tissues can be effected by techniques well known in the art such as described, for example by Foster and Tatlor, Eds. “Plant Virology Protocols: From Virus Isolation to Transgenic Resistance (Methods in Molecular Biology (Humana Pr), VoI 81)”, Humana Press, 1998. Briefly, tissues of an infected plant believed to contain a high concentration of a suitable virus, preferably young leaves and flower petals, are ground in a buffer solution (e.g., phosphate buffer solution) to produce a virus infected sap which can be used in subsequent inoculations.

Construction of plant RNA viruses for the introduction and expression of non-viral exogenous polynucleotide sequences in plants is demonstrated by the above references as well as by Dawson, W. O. et al, Virology (1989) 172:285-292; Takamatsu et al. EMBO J. (1987) 6:307-311; French et al. Science (1986) 231:1294-1297; Takamatsu et al. FEBS Letters (1990) 269:73-76; and U.S. Pat. No. 5,316,931.

When the virus is a DNA virus, suitable modifications can be made to the virus itself. Alternatively, the virus can first be cloned into a bacterial plasmid for ease of constructing the desired viral vector with the foreign DNA. The virus can then be excised from the plasmid. If the virus is a DNA virus, a bacterial origin of replication can be attached to the viral DNA, which is then replicated by the bacteria. Transcription and translation of this DNA will produce the coat proteins which will encapsidate the viral DNA. If the virus is an RNA virus, the virus is generally cloned as a cDNA and inserted into a plasmid. The plasmid is then used to make all of the constructions. The RNA virus is then produced by transcribing the viral sequence of the plasmid and translation of the viral genes to produce the coat protein(s) which encapsidate the viral RNA.

In one embodiment, a plant viral nucleic acid is provided in which the native coat protein coding sequence has been deleted from a viral nucleic acid, a non-native plant viral coat protein coding sequence and a non-native promoter, preferably the subgenomic promoter of the non-native coat protein coding sequence, capable of expression in the plant host, packaging of the recombinant plant viral nucleic acid, and ensuring a systemic infection of the host by the recombinant plant viral nucleic acid, has been inserted. Alternatively, the coat protein gene may be inactivated by insertion of the non-native nucleic acid sequence within it, such that a protein is produced. The recombinant plant viral nucleic acid may contain one or more additional non-native subgenomic promoters. Each non-native subgenomic promoter is capable of transcribing or expressing adjacent genes or nucleic acid sequences in the plant host and incapable of recombination with each other and with native subgenomic promoters. Non-native (foreign) nucleic acid sequences may be inserted adjacent the native plant viral subgenomic promoter or the native and a non-native plant viral subgenomic promoters if more than one nucleic acid sequence is included. The non-native nucleic acid sequences are transcribed or expressed in the host plant under control of the subgenomic promoter to produce the desired products.

In a second embodiment, a recombinant plant viral nucleic acid is provided as in the first embodiment except that the native coat protein coding sequence is placed adjacent one of the non-native coat protein subgenomic promoters instead of a non-native coat protein coding sequence.

In a third embodiment, a recombinant plant viral nucleic acid is provided in which the native coat protein gene is adjacent its subgenomic promoter and one or more non-native subgenomic promoters have been inserted into the viral nucleic acid. The inserted non-native subgenomic promoters are capable of transcribing or expressing adjacent genes in a plant host and are incapable of recombination with each other and with native subgenomic promoters. Non-native nucleic acid sequences may be inserted adjacent the non-native subgenomic plant viral promoters such that the sequences are transcribed or expressed in the host plant under control of the subgenomic promoters to produce the desired product.

In a fourth embodiment, a recombinant plant viral nucleic acid is provided as in the third embodiment except that the native coat protein coding sequence is replaced by a non-native coat protein coding sequence.

The viral vectors are encapsidated by the coat proteins encoded by the recombinant plant viral nucleic acid to produce a recombinant plant virus. The recombinant plant viral nucleic acid or recombinant plant virus is used to infect appropriate host plants. The recombinant plant viral nucleic acid is capable of replication in the host, systemic spread in the host, and transcription or expression of foreign gene(s) (isolated nucleic acid) in the host to produce the desired protein.

In addition to the above, the nucleic acid molecule of the present invention can also be introduced into a chloroplast genome thereby enabling chloroplast expression.

A technique for introducing exogenous nucleic acid sequences to the genome of the chloroplasts is known. This technique involves the following procedures. First, plant cells are chemically treated so as to reduce the number of chloroplasts per cell to about one. Then, the exogenous nucleic acid is introduced via particle bombardment into the cells with the aim of introducing at least one exogenous nucleic acid molecule into the chloroplasts. The exogenous nucleic acid is selected such that it is integratable into the chloroplast's genome via homologous recombination which is readily effected by enzymes inherent to the chloroplast. To this end, the exogenous nucleic acid includes, in addition to a gene of interest, at least one nucleic acid stretch which is derived from the chloroplast's genome. In addition, the exogenous nucleic acid includes a selectable marker, which serves by sequential selection procedures to ascertain that all or substantially all of the copies of the chloroplast genomes following such selection will include the exogenous nucleic acid. Further details relating to this technique are found in U.S. Pat. Nos. 4,945,050; and 5,693,507 which are incorporated herein by reference. A polypeptide can thus be produced by the protein expression system of the chloroplast and become integrated into the chloroplast's inner membrane.

Since NUE, abiotic stress tolerance as well as yield, vigor or biomass of the plant can involve multiple genes acting additively or in synergy (see, for example, in Quesda et al., Plant Physiol. 130:951-063, 2002), the invention also envisages expressing a plurality of exogenous polynucleotides in a single host plant to thereby achieve superior effect on NUE, abiotic stress tolerance, yield, vigor and biomass of the plant.

Expressing a plurality of exogenous polynucleotides in a single host plant can be effected by co-introducing multiple nucleic acid constructs, each including a different exogenous polynucleotide, into a single plant cell. The transformed cell can then be regenerated into a mature plant using the methods described hereinabove. Alternatively, expressing a plurality of exogenous polynucleotides in a single host plant can be effected by co-introducing into a single plant-cell a single nucleic-acid construct including a plurality of different exogenous polynucleotides. Such a construct can be designed with a single promoter sequence which can transcribe a polycistronic messenger RNA including all the different exogenous polynucleotide sequences. To enable co-translation of the different polypeptides encoded by the polycistronic messenger RNA, the polynucleotide sequences can be inter-linked via an internal ribosome entry site (IRES) sequence which facilitates translation of polynucleotide sequences positioned downstream of the IRES sequence. In this case, a transcribed polycistronic RNA molecule encoding the different polypeptides described above will be translated from both the capped 5′ end and the two internal IRES sequences of the polycistronic RNA molecule to thereby produce in the cell all different polypeptides. Alternatively, the construct can include several promoter sequences each linked to a different exogenous polynucleotide sequence.

The plant cell transformed with the construct including a plurality of different exogenous polynucleotides can be regenerated into a mature plant, using the methods described hereinabove.

Alternatively, expressing a plurality of exogenous polynucleotides can be effected by introducing different nucleic acid constructs, including different exogenous polynucleotides, into a plurality of plants. The regenerated transformed plants can then be cross-bred and resultant progeny selected for superior NUE, abiotic stress tolerance, yield as described above, using conventional plant breeding techniques.

As mentioned, expression (or reduction in a level of expression) of the target polynucleotides/polypeptides of the present invention can be qualified using methods which are well known in the art such as those involving gene amplification Western blotting, ELISA, or at the mRNA level involving e.g., PCR or RT-PCR or Northern blot or in-situ hybridization (in which one monitors the level of target gene expression).

According to some embodiments of the invention, the plant expressing the exogenous polynucleotide(s) is grown under stress (nitrogen or abiotic) or normal conditions (e.g., biotic conditions and/or conditions with sufficient water, nutrients such as nitrogen and fertilizer). Such conditions, which depend on the plant being grown, are known to those skilled in the art of agriculture, and are further, described above.

According to some embodiments of the invention, the method further comprises growing the plant expressing the exogenous polynucleotide(s) under abiotic stress or nitrogen limiting conditions. Non-limiting examples of abiotic stress conditions include, water deprivation, drought, excess of water (e.g., flood, waterlogging), freezing, low temperature, high temperature, strong winds, heavy metal toxicity, anaerobiosis, nutrient deficiency, nutrient excess, salinity, atmospheric pollution, intense light, insufficient light, or UV irradiation, etiolation and atmospheric pollution.

Thus, the invention encompasses plants exogenously expressing the polynucleotide(s), the nucleic acid constructs of the invention.

The sequence information and annotations uncovered by the present teachings can be harnessed in favor of classical breeding. Thus, sub-sequence data of those polynucleotides described above, can be used as markers for marker assisted selection (MAS), in which a marker is used for indirect selection of a genetic determinant or determinants of a trait of interest (e.g., tolerance to abiotic stress). Nucleic acid data of the present teachings (DNA or RNA sequence) may contain or be linked to polymorphic sites or genetic markers on the genome such as restriction fragment length polymorphism (RFLP), microsatellites and single nucleotide polymorphism (SNP), DNA fingerprinting (DFP), amplified fragment length polymorphism (AFLP), expression level polymorphism, and any other polymorphism at the DNA or RNA sequence.

Examples of marker assisted selections include, but are not limited to, selection for a morphological trait (e.g., a gene that affects form, coloration, male sterility or resistance such as the presence or absence of awn, leaf sheath coloration, height, grain color, aroma of rice); selection for a biochemical trait (e.g., a gene that encodes a protein that can be extracted and observed; for example, isozymes and storage proteins); selection for a biological trait (e.g., pathogen races or insect biotypes based on host pathogen or host parasite interaction can be used as a marker since the genetic constitution of an organism can affect its susceptibility to pathogens or parasites).

The polynucleotides described hereinabove can be used in a wide range of economical plants, in a safe and cost effective manner.

Plant lines exogenously expressing the polynucleotide of the invention can be screened to identify those that show the greatest increase of the desired plant trait.

Thus, according to an additional embodiment of the present invention, there is provided a method of evaluating a trait of a plant, the method comprising: (a) expressing in a plant or a portion thereof the nucleic acid construct; and (b) evaluating a trait of a plant as compared to a wild type plant of the same type; thereby evaluating the trait of the plant.

Thus, the effect of the transgene (the exogenous polynucleotide) on different plant characteristics may be determined any method known to one of ordinary skill in the art.

Thus, for example, tolerance to limiting nitrogen conditions may be compared in transformed plants {i.e., expressing the transgene) compared to non-transformed (wild type) plants exposed to the same stress conditions (other stress conditions are contemplated as well, e.g. water deprivation, salt stress e.g. salinity, suboptimal temperature, osmotic stress, and the like), using the following assays.

Methods of qualifying plants as being tolerant or having improved tolerance to abiotic stress or limiting nitrogen levels are well known in the art and are further described hereinbelow.

Fertilizer use efficiency—To analyze whether the transgenic plants are more responsive to fertilizers, plants are grown in agar plates or pots with a limited amount of fertilizer, as described, for example, in Yanagisawa et al (Proc Natl Acad Sci USA. 2004; 101:7833-8). The plants are analyzed for their overall size, time to flowering, yield, protein content of shoot and/or grain. The parameters checked are the overall size of the mature plant, its wet and dry weight, the weight of the seeds yielded, the average seed size and the number of seeds produced per plant. Other parameters that may be tested are: the chlorophyll content of leaves (as nitrogen plant status and the degree of leaf verdure is highly correlated), amino acid and the total protein content of the seeds or other plant parts such as leaves or shoots, oil content, etc. Similarly, instead of providing nitrogen at limiting amounts, phosphate or potassium can be added at increasing concentrations. Again, the same parameters measured are the same as listed above. In this way, nitrogen use efficiency (NUE), phosphate use efficiency (PUE) and potassium use efficiency (KUE) are assessed, checking the ability of the transgenic plants to thrive under nutrient restraining conditions.

Nitrogen use efficiency—To analyze whether the transgenic plants (e.g., Arabidopsis plants) are more responsive to nitrogen, plant are grown in 0.75-3 millimolar (mM, nitrogen deficient conditions) or 6-10 mM (optimal nitrogen concentration). Plants are allowed to grow for additional 25 days or until seed production. The plants are then analyzed for their overall size, time to flowering, yield, protein content of shoot and/or grain/seed production. The parameters checked can be the overall size of the plant, wet and dry weight, the weight of the seeds yielded, the average seed size and the number of seeds produced per plant. Other parameters that may be tested are: the chlorophyll content of leaves (as nitrogen plant status and the degree of leaf greenness is highly correlated), amino acid and the total protein content of the seeds or other plant parts such as leaves or shoots and oil content. Transformed plants not exhibiting substantial physiological and/or morphological effects, or exhibiting higher measured parameters levels than wild-type plants, are identified as nitrogen use efficient plants.

Nitrogen Use efficiency assay using plantlets—The assay is done according to Yanagisawa-S. et al. with minor modifications (“Metabolic engineering with Dofl transcription factor in plants: Improved nitrogen assimilation and growth under low-nitrogen conditions” Proc. Natl. Acad. Sci. USA 101, 7833-7838). Briefly, transgenic plants which are grown for 7-10 days in 0.5×MS [Murashige-Skoog] supplemented with a selection agent are transferred to two nitrogen-limiting conditions: MS media in which the combined nitrogen concentration (NH₄NO₃ and KNO₃) was 0.75 mM (nitrogen deficient conditions) or 6-15 mM (optimal nitrogen concentration). Plants are allowed to grow for additional 30-40 days and then photographed, individually removed from the Agar (the shoot without the roots) and immediately weighed (fresh weight) for later statistical analysis. Constructs for which only T1 seeds are available are sown on selective media and at least 20 seedlings (each one representing an independent transformation event) are carefully transferred to the nitrogen-limiting media. For constructs for which T2 seeds are available, different transformation events are analyzed. Usually, 20 randomly selected plants from each event are transferred to the nitrogen-limiting media allowed to grow for 3-4 additional weeks and individually weighed at the end of that period. Transgenic plants are compared to control plants grown in parallel under the same conditions. Mock-transgenic plants expressing the uidA reporter gene (GUS) under the same promoter or transgenic plants carrying the same promoter but lacking a reporter gene are used as control.

Nitrogen determination—The procedure for N (nitrogen) concentration determination in the structural parts of the plants involves the potassium persulfate digestion method to convert organic N to NO₃ ⁻ (Purcell and King 1996 Argon. J. 88:111-113, the modified Cd⁻ mediated reduction of NO₃ ⁻ to NO₂ ⁻ (Vodovotz 1996 Biotechniques 20:390-394) and the measurement of nitrite by the Griess assay (Vodovotz 1996, supra). The absorbance values are measured at 550 nm against a standard curve of NaNO₂. The procedure is described in details in Samonte et al. 2006 Agron. J. 98:168-176.

Tolerance to abiotic stress (e.g. tolerance to drought or salinity) can be evaluated by determining the differences in physiological and/or physical condition, including but not limited to, vigor, growth, size, or root length, or specifically, leaf color or leaf area size of the transgenic plant compared to a non-modified plant of the same species grown under the same conditions. Other techniques for evaluating tolerance to abiotic stress include, but are not limited to, measuring chlorophyll fluorescence, photosynthetic rates and gas exchange rates. Further assays for evaluating tolerance to abiotic stress are provided hereinbelow and in the Examples section which follows.

Drought tolerance assay—Soil-based drought screens are performed with plants overexpressing the polynucleotides detailed above. Seeds from control Arabidopsis plants, or other transgenic plants overexpressing nucleic acid of the invention are germinated and transferred to pots. Drought stress is obtained after irrigation is ceased. Transgenic and control plants are compared to each other when the majority of the control plants develop severe wilting. Plants are re-watered after obtaining a significant fraction of the control plants displaying a severe wilting. Plants are ranked comparing to controls for each of two criteria: tolerance to the drought conditions and recovery (survival) following re-watering.

Quantitative parameters of tolerance measured include, but are not limited to, the average wet and dry weight, growth rate, leaf size, leaf coverage (overall leaf area), the weight of the seeds yielded, the average seed size and the number of seeds produced per plant. Transformed plants not exhibiting substantial physiological and/or morphological effects, or exhibiting higher biomass than wild-type plants, are identified as drought stress tolerant plants,

Salinity tolerance assay—Transgenic plants with tolerance to high salt concentrations are expected to exhibit better germination, seedling vigor or growth in high salt. Salt stress can be effected in many ways such as, for example, by irrigating the plants with a hyperosmotic solution, by cultivating the plants hydroponically in a hyperosmotic growth solution (e.g., Hoagland solution with added salt), or by culturing the plants in a hyperosmotic growth medium [e.g., 50% Murashige-Skoog medium (MS medium) with added salt]. Since different plants vary considerably in their tolerance to salinity, the salt concentration in the irrigation water, growth solution, or growth medium can be adjusted according to the specific characteristics of the specific plant cultivar or variety, so as to inflict a mild or moderate effect on the physiology and/or morphology of the plants (for guidelines as to appropriate concentration see, Bernstein and Kafkafi, Root Growth Under Salinity Stress In: Plant Roots, The Hidden Half 3rd ed. Waisel Y, Eshel A and Kafkafi U. (editors) Marcel Dekker Inc., New York, 2002, and reference therein).

For example, a salinity tolerance test can be performed by irrigating plants at different developmental stages with increasing concentrations of sodium chloride (for example 50 mM, 150 mM, 300 mM NaCl) applied from the bottom and from above to ensure even dispersal of salt. Following exposure to the stress condition the plants are frequently monitored until substantial physiological and/or morphological effects appear in wild type plants. Thus, the external phenotypic appearance, degree of chlorosis and overall success to reach maturity and yield progeny are compared between control and transgenic plants. Quantitative parameters of tolerance measured include, but are not limited to, the average wet and dry weight, growth rate, leaf size, leaf coverage (overall leaf area), the weight of the seeds yielded, the average seed size and the number of seeds produced per plant. Transformed plants not exhibiting substantial physiological and/or morphological effects, or exhibiting higher biomass than wild-type plants, are identified as abiotic stress tolerant plants.

Osmotic tolerance test—Osmotic stress assays (including sodium chloride and PEG assays) are conducted to determine if an osmotic stress phenotype was sodium chloride-specific or if it was a general osmotic stress related phenotype. Plants which are tolerant to osmotic stress may have more tolerance to drought and/or freezing. For salt and osmotic stress experiments, the medium is supplemented for example with 50 mM, 100 mM, 200 mM NaCl or 15%, 20% or 25% PEG.

Cold stress tolerance—One way to analyze cold stress is as follows. Mature (25 day old) plants are transferred to 4° C. chambers for 1 or 2 weeks, with constitutive light. Later on plants are moved back to greenhouse. Two weeks later damages from chilling period, resulting in growth retardation and other phenotypes, are compared between control and transgenic plants, by measuring plant weight (wet and dry), and by comparing growth rates measured as time to flowering, plant size, yield, and the like.

Heat stress tolerance—One way to measure heat stress tolerance is by exposing the plants to temperatures above 34° C. for a certain period. Plant tolerance is examined after transferring the plants back to 22° C. for recovery and evaluation after 5 days relative to internal controls (non-transgenic plants) or plants not exposed to neither cold or heat stress.

The biomass, vigor and yield of the plant can also be evaluated using any method known to one of ordinary skill in the art. Thus, for example, plant vigor can be calculated by the increase in growth parameters such as leaf area, fiber length, rosette diameter, plant fresh weight and the like per time.

As mentioned, the increase of plant yield can be determined by various parameters. For example, increased yield of rice may be manifested by an increase in one or more of the following: number of plants per growing area, number of panicles per plant, number of spikelets per panicle, number of flowers per panicle, increase in the seed filling rate, increase in thousand kernel weight (1000-weight), increase oil content per seed, increase starch content per seed, among others. An increase in yield may also result in modified architecture, or may occur because of modified architecture. Similarly, increased yield of soybean may be manifested by an increase in one or more of the following: number of plants per growing area, number of pods per plant, number of seeds per pod, increase in the seed filling rate, increase in thousand seed weight (1000-weight), reduce pod shattering, increase oil content per seed, increase protein content per seed, among others. An increase in yield may also result in modified architecture, or may occur because of modified architecture.

Thus, the present invention is of high agricultural value for increasing tolerance of plants to nitrogen deficiency or abiotic stress as well as promoting the yield, biomass and vigor of commercially desired crops.

According to another embodiment of the present invention, there is provided a food or feed comprising the plants or a portion thereof of the present invention.

In a further aspect the invention, the transgenic plants of the present invention or parts thereof are comprised in a food or feed product (e.g., dry, liquid, paste). A food or feed product is any ingestible preparation containing the transgenic plants, or parts thereof, of the present invention, or preparations made from these plants. Thus, the plants or preparations are suitable for human (or animal) consumption, i.e. the transgenic plants or parts thereof are more readily digested. Feed products of the present invention further include a oil or a beverage adapted for animal consumption.

It will be appreciated that the transgenic plants, or parts thereof, of the present invention may be used directly as feed products or alternatively may be incorporated or mixed with feed products for consumption. Furthermore, the food or feed products may be processed or used as is. Exemplary feed products comprising the transgenic plants, or parts thereof, include, but are not limited to, grains, cereals, such as oats, e.g. black oats, barley, wheat, rye, sorghum, corn, vegetables, leguminous plants, especially soybeans, root vegetables and cabbage, or green forage, such as grass or hay.

As used herein the term “about” refers to ±10%.

The terms “comprises”, “comprising”, “includes”, “including”, “having” and their conjugates mean “including but not limited to”.

The term “consisting of” means “including and limited to”.

The term “consisting essentially of” means that the composition, method or structure may include additional ingredients, steps and/or parts, but only if the additional ingredients, steps and/or parts do not materially alter the basic and novel characteristics of the claimed composition, method or structure.

As used herein, the singular form “a”, “an” and “the” include plural references unless the context clearly dictates otherwise. For example, the term “a compound” or “at least one compound” may include a plurality of compounds, including mixtures thereof.

Throughout this application, various embodiments of this invention may be presented in a range format. It should be understood that the description in range format is merely for convenience and brevity and should not be construed as an inflexible limitation on the scope of the invention. Accordingly, the description of a range should be considered to have specifically disclosed all the possible subranges as well as individual numerical values within that range. For example, description of a range such as from 1 to 6 should be considered to have specifically disclosed subranges such as from 1 to 3, from 1 to 4, from 1 to 5, from 2 to 4, from 2 to 6, from 3 to 6 etc., as well as individual numbers within that range, for example, 1, 2, 3, 4, 5, and 6. This applies regardless of the breadth of the range.

Whenever a numerical range is indicated herein, it is meant to include any cited numeral (fractional or integral) within the indicated range. The phrases “ranging/ranges between” a first indicate number and a second indicate number and “ranging/ranges from” a first indicate number “to” a second indicate number are used herein interchangeably and are meant to include the first and second indicated numbers and all the fractional and integral numerals therebetween.

As used herein the term “method” refers to manners, means, techniques and procedures for accomplishing a given task including, but not limited to, those manners, means, techniques and procedures either known to, or readily developed from known manners, means, techniques and procedures by practitioners of the chemical, pharmacological, biological, biochemical and medical arts.

As used herein, the term “treating” includes abrogating, substantially inhibiting, slowing or reversing the progression of a condition, substantially ameliorating clinical or aesthetical symptoms of a condition or substantially preventing the appearance of clinical or aesthetical symptoms of a condition.

It is appreciated that certain features of the invention, which are, for clarity, described in the context of separate embodiments, may also be provided in combination in a single embodiment. Conversely, various features of the invention, which are, for brevity, described in the context of a single embodiment, may also be provided separately or in any suitable subcombination or as suitable in any other described embodiment of the invention. Certain features described in the context of various embodiments are not to be considered essential features of those embodiments, unless the embodiment is inoperative without those elements.

Various embodiments and aspects of the present invention as delineated hereinabove and as claimed in the claims section below find experimental support in the following examples.

EXAMPLES

Reference is now made to the following examples, which together with the above descriptions illustrate some embodiments of the invention in a non limiting fashion.

Generally, the nomenclature used herein and the laboratory procedures utilized in the present invention include molecular, biochemical, microbiological and recombinant DNA techniques. Such techniques are thoroughly explained in the literature. See, for example, “Molecular Cloning: A laboratory Manual” Sambrook et al., (1989); “Current Protocols in Molecular Biology” Volumes I-III Ausubel, R. M., ed. (1994); Ausubel et al., “Current Protocols in Molecular Biology”, John Wiley and Sons, Baltimore, Md. (1989); Perbal, “A Practical Guide to Molecular Cloning”, John Wiley & Sons, New York (1988); Watson et al., “Recombinant DNA”, Scientific American Books, New York; Birren et al. (eds) “Genome Analysis: A Laboratory Manual Series”, Vols. 1-4, Cold Spring Harbor Laboratory Press, New York (1998); methodologies as set forth in U.S. Pat. Nos. 4,666,828; 4,683,202; 4,801,531; 5,192,659 and 5,272,057; “Cell Biology: A Laboratory Handbook”, Volumes I-III Cellis, J. E., ed. (1994); “Current Protocols in Immunology” Volumes I-III Coligan J. E., ed. (1994); Stites et al. (eds), “Basic and Clinical Immunology” (8th Edition), Appleton & Lange, Norwalk, Conn. (1994); Mishell and Shiigi (eds), “Selected Methods in Cellular Immunology”, W. H. Freeman and Co., New York (1980); available immunoassays are extensively described in the patent and scientific literature, see, for example, U.S. Pat. Nos. 3,791,932; 3,839,153; 3,850,752; 3,850,578; 3,853,987; 3,867,517; 3,879,262; 3,901,654; 3,935,074; 3,984,533; 3,996,345; 4,034,074; 4,098,876; 4,879,219; 5,011,771 and 5,281,521; “Oligonucleotide Synthesis” Gait, M. J., ed. (1984); “Nucleic Acid Hybridization” Hames, B. D., and Higgins S. J., eds. (1985); “Transcription and Translation” Hames, B. D., and Higgins S. J., Eds. (1984); “Animal Cell Culture” Freshney, R. I., ed. (1986); “Immobilized Cells and Enzymes” IRL Press, (1986); “A Practical Guide to Molecular Cloning” Perbal, B., (1984) and “Methods in Enzymology” Vol. 1-317, Academic Press; “PCR Protocols: A Guide To Methods And Applications”, Academic Press, San Diego, Calif. (1990); Marshak et al., “Strategies for Protein Purification and Characterization—A Laboratory Course Manual” CSHL Press (1996); all of which are incorporated by reference as if fully set forth herein. Other general references are provided throughout this document. The procedures therein are believed to be well known in the art and are provided for the convenience of the reader. All the information contained therein is incorporated herein by reference.

Example 1 Differential Expression of dsRNAs in Maize Plant Under Optimal Versus Deficient Nitrogen Conditions

Experimental Procedures

Plant Material

Corn seeds were obtained from Galil seeds (Israel). Corn variety 5605 was used in all experiments. Plants were grown at 24° C. under a 16 hr light:8 hr dark regime.

Stress Induction

Corn seeds were germinated and grown on agar with defined growth media containing either optimal (100% N₂, 20.61 mM) or suboptimal nitrogen levels (1% or 10% N₂, 0.2 mM or 2.06 mM, respectively). Seedlings aged one or two weeks were used for tissue samples for RNA analysis, as described below.

Total RNA Extraction

Total RNA of leaf or root samples from four to eight biological repeats were extracted using the mirVana™ kit (Ambion, Austin, Tex.) by pooling 3-4 plants to one biological repeat.

Microarray Design

Custom microarrays were manufactured by Agilent Technologies by in situ synthesis. The first generation microarray consisted of a total of 13619 non-redundant DNA probes, the majority of which arose from deep sequencing data and includes different small RNA molecules (i.e. miRNAs, siRNA and predicted small RNA sequences), with each probe being printed once. An in-depth analysis of the first generation microarray, which included hybridization experiments as well as structure and orientation verifications on all its small RNAs, resulted in the formation of an improved, second generation, microarray. The second generation microarray consists of a total 4721 non-redundant DNA 45-nucleotide long probes for all known plant small RNAs, with 912 sequences (19.32%) from Sanger version 15 and the rest (3809), encompassing miRNAs (968=20.5%), siRNAs (1626=34.44%) and predicted small RNA sequences (1215=25.74%), from deep sequencing data accumulated by the inventors, with each probe being printed in triplicate.

Results

Wild type maize plants were allowed to grow at standard, optimal conditions or nitrogen deficient conditions for one or two weeks, at the end of which they were evaluated for NUE. Three to four plants from each group were used for reproducibility. Four to eight repeats were obtained for each group and RNA was extracted from leaf or root tissue. The expression level of the maize miRNAs was analyzed by high throughput microarray to identify miRNAs that were differentially expressed between the experimental groups.

Tables 1-4 below present dsRNA sequences that were found to be differentially expressed (upregulated=up; downregulated=down) in corn grown under low nitrogen conditions (nitrogen limiting conditions, as described above).

TABLE 1 miRNAs found upregulated in Plants Growing under Nitrogen Deficient Conditions versus Optimal conditions Stem Fold Fold Loop SEQ Change Change Mir Name SEQ ID NO: ID NO: Direction Leaf Root osa-miR1430 TGGTGAGCCTTCCTGGC 196 Up 3.99 TAAG/1 osa-miR1868 TCACGGAAAACGAGGG 197 Up 2.63 AGCAGCCA/2 osa-miR2096-3p CCTGAGGGGAAATCGG 198 Up 3.48 2.71 CGGGA/3 zma-miR399f* GGGCAACTTCTCCTTTG 199 Up 2.13 GCAGA/4 Predicted folded AACTAAAACGAAACGG 200 Up 2.1 24-nts-long seq AAGGAGTA/5 50935 Predicted folded AAGGTGCTTTTAGGAGT 201 Up 2.08 24-nts-long seq AGGACGG/6 51052 Predicted folded ACAAAGGAATTAGAAC 202 Up 3.23 2.49 24-nts-long seq GGAATGGC/7 51215 Predicted folded AGAATCAGGAATGGAA 203 Up 1.54 24-nts-long seq CGGCTCCG/8 51468 Predicted folded AGAATCAGGGATGGAA 204 Up 1.9 24-nts-long seq CGGCTCTA/9 51469 Predicted folded AGAGTCACGGGCGAGA 205 Up 2.34 24-nts-long seq AGAGGACG/10 51577 Predicted folded AGGACCTAGATGAGCG 206 Up 1.72 24-nts-long seq GGCGGTTT/11 51691 Predicted folded AGGACGCTGCTGGAGA 207 Up 2.4 24-nts-long seq CGGAGAAT/12 51695 Predicted folded AGGGCTTGTTCGGTTTG 208 Up 2.52 24-nts-long seq AAGGGGT/13 51814 Predicted folded ATCTTTCAACGGCTGCG 209 Up 2.11 24-nts-long seq AAGAAGG/14 52057 Predicted folded CTAGAATTAGGGATGG 210 Up 1.57 24-nts-long seq AACGGCTC/15 52327 Predicted folded GAGGGATAACTGGGGA 211 Up 2.97 24-nts-long seq CAACACGG/16 52499 Predicted folded GCGGAGTGGGATGGGG 212 Up 1.51 24-nts-long seq AGTGTTGC/17 52633 Predicted folded GGAGACGGATGCGGAG 213 Up 1.51 24-nts-long seq ACTGCTGG/18 52688 Predicted folded GGTTAGGAGTGGATTG 214 Up 3.77 24-nts-long seq AGGGGGAT/19 52805 Predicted folded GTCAAGTGACTAAGAG 215 Up 4.93 10.17 24-nts-long seq CATGTGGT/20 52850 Predicted folded GTGGAATGGAGGAGAT 216 Up 2.01 24-nts-long seq TGAGGGGA/21 52882 Predicted folded TGGCTGAAGGCAGAAC 217 Up 4.45 24-nts-long seq CAGGGGAG/22 53118 Predicted folded TGTGGTAGAGAGGAAG 218 Up 3.25 24-nts-long seq AACAGGAC/23 53149 Predicted folded AGGGACTCTCTTTATTT 219 Up 1.83 24-nts-long seq CCGACGG/24 53594 Predicted folded AGGGTTCGTTTCCTGGG 220 Up 1.66 24-nts-long seq AGCGCGG/25 53604 Predicted folded TCCTAGAATCAGGGAT 221 Up 1.6 24-nts-long seq GGAACGGC/26 54081 Predicted folded TGGGAGCTCTCTGTTCG 222 Up 3.47 24-nts-long seq ATGGCGC/27 54132 Predicted zma AACGTCGTGTCGTGCTT 223 Up 1.62 mir 48061 GGGCT/28 Predicted zma ACCTGGACCAATACAT 224 Up 2.58 mir 48295 GAGATT/29 Predicted zma AGAAGCGACAATGGGA 225 Up 4.65 mir 48350 CGGAGT/30 Predicted zma AGGAAGGAACAAACGA 226 Up 2.08 mir 48457 GGATAAG/31 Predicted zma CCAAGAGATGGAAGGG 227 Up 2 mir 48877 CAGAGC/32 Predicted zma CGACAACGGGACGGAG 228 Up 1.58 mir 48922 TTCAA/33 Predicted zma GAGGATGGAGAGGTAC 229 Up 2.02 mir 49123 GTCAGA/34 Predicted zma GATGGGTAGGAGAGCG 230 Up 1.51 1.55 mir 49161 TCGTGTG/35 Predicted zma GATGGTTCATAGGTGA 231 Up 4.2 mir 49162 CGGTAG/36 Predicted zma GGGAGCCGAGACATAG 232 Up 2.64 mir 49262 AGATGT/37 Predicted zma GTGAGGAGTGATAATG 233 Up 2.17 mir 49323 AGACGG/38 Predicted zma GTTTGGGGCTTTAGCAG 234 Up 1.58 mir 49369 GTTTAT/39 Predicted zma TCCATAGCTGGGCGGA 235 Up 5.52 mir 49609 AGAGAT/40 Predicted zma TCGGCATGTGTAGGAT 236 Up 3.24 ± 1.00  3.235 ± 0.205 mir 49638 AGGTG/41 Predicted zma TGATAGGCTGGGTGTG 237 Up 2.01 1.73 mir 49761 GAAGCG/42 Predicted zma TGCAAACAGACTGGGG 238 Up 3 mir 49787 AGGCGA/43 Predicted zma TTTGGCTGACAGGATA 239 Up 2.44 mir 50077 AGGGAG/44 Predicted zma TTTTCATAGCTGGGCGG 240 Up 19.94 mir 50095 AAGAG/45 Predicted zma AACTTTAAATAGGTAG 241 Up 1.51 mir 50110 GACGGCGC/46 Predicted zma GGAATGTTGTCTGGTTC 242 Up 14.34 mir 50204 AAGG/47 Predicted zma TGTAATGTTCGCGGAA 243 Up 1.7 mir 50261 GGCCAC/48 Predicted zma TGTTGGCATGGCTCAAT 244 Up 1.82 mir 50267 CAAC/49 Predicted zma CGCTGACGCCGTGCCA 245 Up 2.33 mir 50460 CCTCAT/50 Predicted zma GCCTGGGCCTCTTTAGA 246 Up 1.5 mir 50545 CCT/51 Predicted zma GTAGGATGGATGGAGA 247 Up 2.07 mir 50578 GGGTTC/52 Predicted zma TCAACGGGCTGGCGGA 248 Up 1.55 mir 50611 TGTG/53 Table 1. provided are miRNAs that were found upregulated in Plants Growing under Nitrogen Deficient Conditions versus Optimal conditions.

TABLE 2 miRNAs found downregulated in Plants Growing under Nitrogen Deficient Conditions versus Optimal conditions Stem Loop Fold Fold Mature Sequence/ Change Change Mir Name Sequence/seq id no: seq id no: Direction Leaf Root aqc- AGAAGAGAGAG 249 Down 1.53 miR529 AGCACAACCC/54 ath- CTTGAGAGAGAG 250 Down 1.54 miR2936 AACACAGACG/55 mtr- TGAGCCAGGATG 251 Down 3.04 miR169q ACTTGCCGG/56 peu- GGCCGGGGGACG 254 Down 1.66 miR2911 GGCTGGGA/59 Predicted AAAAAAGACTGA 255 Down 2.66 folded 24- GCCGAATTGAAA/ nts-long 60 seq 50703 Predicted AAGGAGTTTAAT 256 Down 1.62 folded 24- GAAGAAAGAGA nts-long G/61 seq 51022 Predicted ACTGATGACGAC 257 Down 7.7 folded 24- ACTGAGGAGGCT/ nts-long 62 seq 51381 Predicted AGAGGAACCAGA 258 Down 1.52 folded 24- GCCGAAGCCGTT/ nts-long 63 seq 51542 Predicted AGGCAAGGTGGA 259 Down 2.07 folded 24- GGACGTTGATGA/ nts-long 64 seq 51757 Predicted AGGGCTGATTTG 260 Down 3.7 2.04 folded 24- GTGACAAGGGGA/ nts-long 65 seq 51802 Predicted ATATAAAGGGAG folded 24- GAGGTATGGACC/ 261 Down 2.1 nts-long 66 seq 51966 Predicted ATCGGTCAGCTG 262 Down 1.7 folded 24- GAGGAGACAGGT/ nts-long 67 seq 52041 Predicted ATGGTAAGAGAC 263 Down 1.62 folded 24- TATGATCCAACT/ nts-long 68 seq 52109 Predicted CAATTTTGTACT 264 Down 1.53 folded 24- GGATCGGGGCAT/ nts-long 69 seq 52212 Predicted CAGAGGAACCAG 265 Down 1.58 folded 24- AGCCGAAGCCGT/ nts-long 70 seq 52218 Predicted CGGCTGGACAGG 266 Down 1.63 folded 24- GAAGAAGAGCAC/ nts-long 71 seq 52299 Predicted GAAACTTGGAGA 267 Down 1.7 folded 24- GATGGAGGCTTT/ nts-long 72 seq 52347 Predicted GAGAGAGAAGG 268 Down 3.25 2.52 folded 24- GAGCGGATCTGG nts-long T/73 seq 52452 Predicted GCTGCACGGGAT 269 Down 2.34 folded 24- TGGTGGAGAGGT/ nts-long 74 seq 52648 Predicted GGCTGCTGGAGA 270 Down 2.13 folded 24- GCGTAGAGGACC/ nts-long 75 seq 52739 Predicted GGGTTTTGAGAG 271 Down 2.9 folded 24- CGAGTGAAGGGG/ nts-long 76 seq 52792 Predicted GGTATTGGGGTG 272 Down 1.59 folded 24- GATTGAGGTGGA/ nts-long 77 seq 52795 Predicted GGTGGCGATGCA 273 Down 2.52 3.87 folded 24- AGAGGAGCTCAA/ nts-long 78 seq 52801 Predicted GTTGCTGGAGAG 274 Down 2.35 folded 24- AGTAGAGGACGT/ nts-long 79 seq 52955 Predicted AAAAGAGAAACC 275 Down 1.78 zma mir GAAGACACAT/80 47944 Predicted AAAGAGGATGAG 276 Down 4.09 zma mir GAGTAGCATG/81 47976 Predicted AATACACATGGG 277 Down 1.85 zma mir TTGAGGAGG/82 48185 Predicted AGAAGCGGACTG 278 Down 3.18 zma mir CCAAGGAGGC/83 48351 Predicted AGAGGGTTTGGG 279 Down 8.95 zma mir GATAGAGGGAC/84 48397 Predicted TAAGGGATGAGG 280 Down 2.1 zma mir CAGAGCATG/85 48588 Predicted ATGCTATTTGTA 281 Down 1.67 zma mir CCCGTCACCG/86 48669 Predicted ATGTGGATAAAA 282 Down 1.61 zma mir GGAGGGATGA/87 48708 Predicted CAACAGGAACAA 283 Down 1.52 zma mir GGAGGACCAT/88 48771 Predicted CTCGAGTTGAGAA 284 Down 1.51 zma mir AGAGATGCT/89 49002 Predicted CTCGATGGGAGGT 285 Down 1.61 zma mir GGAGTTGCAT/90 49003 Predicted CTGGGAAGATGG 286 Down 1.64 zma mir AACATTTTGGT/91 49011 Predicted GAAGATATACGA 287 Down 1.55 zma mir TGATGAGGAG/92 49053 Predicted GAATCTATCGTT 288 Down 1.65 2.01 zma mir TGGGCTCAT/93 49070 Predicted GAGCGAGCTACAA 289 Down 1.6 zma mir AAGGATTCG/94 49082 Predicted GAGTGACGAGGAG 290 Down 3.64 zma mir TGAGAGTAGG/95 49155 Predicted GGGCATCTTCTG 291 Down 1.64 zma mir GCAGGAGGACA/ 49269 96 Predicted TACGGAAGAAGA 292 Down 1.64 zma mir GCAAGTTTT/97 49435 Predicted TAGAAAGAGCGA 293 Down 1.55 zma mir GAGAACAAAG/98 49445 Predicted TGATATTATGGA 294 Down 1.54 1.57 zma mir CGACTGGTT/99 49762 Predicted TGGAAGGGCCAT 295 Down 2.45 zma mir GCCGAGGAG/100 49816 Predicted TTGAGCGCAGCG 296 Down 2.93 zma mir TTGATGAGC/101 49985 Predicted TTGGATAACGGG 297 Down 1.79 zma mir TAGTTTGGAGT/ 50021 102 Predicted AGCTGCCGACTC 298 Down 1.54 zma mir ATTCACCCA/103 50144 Predicted TGTACGATGATC 299 Down 1.53 zma mir AGGAGGAGGT/ 50263 104 Predicted TGTGTTCTCAGG 300 Down 2.51 zma mir TCGCCCCCG/105 50266 Predicted ACTAAAAAGAAA 301 Down 1.5 zma mir CAGAGGGAG/106 50318 Predicted GACCGGCTCGAC 302 Down 1.55 zma mir CCTTCTGC/107 50517 Predicted TGGTAGGATGGA 303 Down 1.55 zma mir TGGAGAGGGT/ 50670 108 zma- GGAATGTTGTCT 304 Down 1.73 miR166d* GGTTCAAGG/109 zma- GGCAAGTCTGTC 305 Down 2.41 miRl69c* CTTGGCTACA/110 zma- TGCCAAAGGGGA 309 Down 1.55 miR399g TTTGCCCGG/113 Table 2. provided are miRNAs that were found downregulated in Plants Growing under Nitrogen Deficient Conditions versus Optimal conditions.

TABLE 3 siRNAs found upregulated in Plants Growing under Nitrogen Deficient Conditions versus Optimal conditions Fold Fold Change Mir Name Mature Sequence/SEQ ID NO: Direction Change Leaf Root Predicted AAGAAACGGGGCAGTGAGA Up 1.51 siRNA 54339 TGGAC/114 Predicted AGAAAAGATTGAGCCGAAT Up 2.02 siRNA 54631 TGAATT/115 Predicted AGAGCCTGTAGCTAATGGT Up 1.95 siRNA 54991 GGG/116 Predicted AGGTAGCGGCCTAAGAACG Up 2.36 1.67 siRNA 55111 ACACA/117 Predicted CCTATATACTGGAACGGAA Up 1.57 siRNA 55423 CGGCT/118 Predicted CTATATACTGGAACGGAAC Up 2.23 siRNA 55806 GGCTT/119 Predicted GACGAGATCGAGTCTGGAG Up 1.86 siRNA 56052 CGAGC/120 Predicted GAGTATGGGGAGGGACTAG Up 2.3 siRNA 56106 GGA/121 Predicted GACGAAATAGAGGCTCAGG Up 2.08 siRNA 56353 AGAGG/122 Predicted GGATTCGTGATTGGCGATG Up 1.51 siRNA 56388 GGG/123 Predicted GGTGAGAAACGGAAAGGCA Up 4.04 siRNA 56406 GGACA/124 Predicted GTGTCTGAGCAGGGTGAGA Up 1.53 1.58 siRNA 56443 AGGCT/125 Predicted GTTTTGGAGGCGTAGGCGA Up 3.04 siRNA 56450 GGGAT/126 Predicted TGGGACGCTGCATCTGTTGA Up 2.96 siRNA 56542 T/127 Predicted TCTATATACTGGAACGGAA Up 1.76 siRNA 56706 CGGCT/128 Predicted GTTGTTGGAGGGGTAGAGG Up 1.55 siRNA 56856 ACGTC/129 Predicted AATGACAGGACGGGATGGG Up 2.87 siRNA 57034 ACGGG/130 Predicted ACGGAACGGCTTCATACCA Up 2.43 siRNA 57054 CAATA/131 Predicted GACGGGCCGACATTTAGAG Up 1.69 siRNA 57193 CACGG/132 Predicted ACGGATAAAAGGTACTCT/ Up 2.82 siRNA 57884 133 Predicted AGTATGTCGAAAACTGGAG Up 4.54 siRNA 58292 GGC/134 Predicted ATAAGCACCGGCTAACTCT/ Up 2.87 siRNA 58362 135 Predicted ATTCAGCGGGCGTGGTTATT Up 1.55 siRNA 58665 GGCA/136 Predicted CAGCGGGTGCCATAGTCGA Up 1.92 siRNA 58872 T/137 Predicted CATTGCGACGGTCCTCAA/ Up 1.57 siRNA 58940 138 Predicted CTCAACGGATAAAAGGTAC/ Up 2.21 siRNA 59380 139 Predicted GACAGTCAGGATGTTGGCT/ Up 2.68 2.12 siRNA 59626 140 Predicted GACTGATCCTTCGGTGTCGG Up 1.67 siRNA 59659 CG/141 Predicted GCCGAAGATTAAAAGACGA Up 1.64 siRNA 59846 GACGA/142 Predicted GCCTTTGCCGACCATCCTGA/ Up 1.6 siRNA 59867 143 Predicted GGAATCGCTAGTAATCGTG Up 1.87 1.76 siRNA 59952 GAT/144 Predicted GGAGCAGCTCTGGTCGTGG Up 1.85 ± 0.007 siRNA 59961 G/145 Predicted GGAGGCTCGACTATGTTCA Up 2.97 siRNA 59965 AA/146 Predicted GGAGGGATGTGAGAACATG Up 1.62 siRNA 59966 GGC/147 Predicted GTCCCCTTCGTCTAGAGGC/ Up 2.82 siRNA 60081 148 Predicted GTCTGAGTGGTGTAGTTGGT/ Up 2.12 siRNA 60095 149 Predicted GTTGGTAGAGCAGTTGGC/ Up 4.11 siRNA 60188 150 Predicted TACGTTCCCGGGTCTTGTAC Up 1.95 siRNA 60285 A/151 Predicted TATGGATGAAGATGGGGGT Up 3.68 siRNA 60387 G/152 Predicted TCAACGGATAAAAGGTACT Up 2.23 siRNA 60434 CCG/153 Predicted TGCCCAGTGCTTTGAATG/ Up 3.37 siRNA 60837 154 Predicted TGCGAGACCGACAAGTCGA Up 1.64 1.86 siRNA 60850 GC/155 Predicted TTTGCGACACGGGCTGCTCT/ Up 1.52 siRNA 61382 156 Table 3. Provided are siRNAs that were found upregulated in Plants Growing under Nitrogen Deficient Conditions versus Optimal conditions.

TABLE 4 siRNAs found downregulated in Plants Growing under Nitrogen Deficient Conditions versus Optimal conditions Fold Fold Change Change Mir Name Mature Sequence/SEQ ID No: Direction Leaf Root Predicted siRNA CATCGCTCAACGGACAAAAG Down 1.55 58924 GT/157 Predicted siRNA AAGACGAAGGTAGCAGCGC Down 2.79 54240 GATAT/158 Predicted siRNA AGCCAGACTGATGAGAGAA Down 1.51 54957 GGAGG/159 Predicted siRNA ACGTTGTTGGAAGGGTAGAG Down 1.56 55081 GACG/160 Predicted siRNA CAAGTTATGCAGTTGCTGCC Down 5.98 55393 T/161 Predicted siRNA CAGAATGGAGGAAGAGATG Down 3.49 55404 GTG/162 Predicted siRNA ATCTGTGGAGAGAGAAGGTT Down 1.58 55472 GCCC/163 Predicted siRNA ATGTCAGGGGGCCATGCAGT Down 2.41 55720 AT/164 Predicted siRNA ATCCTGACTGTGCCGGGCCG Down 1.96 55732 GCCC/165 Predicted siRNA CGAGTTCGCCGTAGAGAAAG Down 2.24 56034 CT/166 Predicted siRNA GACTGATTCGGACGAAGGAG Down 3.23 56162 GGTT/167 Predicted siRNA GTCTGAACACTAAACGAAGC Down 1.87 56205 ACA/168 Predicted siRNA GACGTTGTTGGAAGGGTAGA Down 3.94 56277 GGAC/169 Predicted siRNA GCTACTGTAGTTCACGGGCC Down 1.71 56307 GGCC/170 Predicted siRNA GGTATTCGTGAGCCTGTTTCT Down 1.67 56425 GGTT/171 Predicted siRNA TGGAAGGAGCATGCATCTTG Down 2.68 56837 AG/172 Predicted siRNA TTCTTGACCTTGTAAGACCC Down 3.66 56965 A/173 Predicted siRNA AGCAGAATGGAGGAAGAGA Down 1.53 57088 TGG/174 Predicted siRNA CTGGACACTGTTGCAGAAGG Down 1.58 57179 AGGA/175 Predicted siRNA GAAATAGGATAGGAGGAGG Down 3.34 2.91 57181 GATGA/176 Predicted siRNA GGCACGACTAACAGACTCAC Down 2.45 57228 GGGC/177 Predicted siRNA AATCCCGGTGGAACCTCCA/ Down 3.6 2.7 57685 178 Predicted siRNA ACACGACAAGACGAATGAG Down 1.57 57772 AGAGA/179 Predicted siRNA ACGACGAGGACTTCGAGACG/ Down 1.53 57863 180 Predicted siRNA CAAAGTGGTCGTGCCGGAG/ Down 1.61 58721 181 Predicted siRNA CAGCTTGAGAATCGGGCCGC/ Down 3.8 58877 182 Predicted siRNA CCCTGTGACAAGAGGAGGA/ Down 1.6 59032 183 Predicted siRNA CCTGCTAACTAGTTATGCGG Down 1.74 59102 AGC/184 Predicted siRNA CGAACTCAGAAGTGAAACC/ Down 2.11 2.62 59123 185 Predicted siRNA CGCTTCGTCAAGGAGAAGGG Down 1.59 59235 C/186 Predicted siRNA CTTAACTGGGCGTTAAGTTG Down 2.17 59485 CAGGGT/187 Predicted siRNA GGACGAACCTCTGGTGTACC/ Down 1.76 59954 188 Predicted siRNA GGCGCTGGAGAACTGAGGG/ Down 2.58 59993 189 Predicted siRNA GGGGGCCTAAATAAAGACT/ Down 2.48 60012 190 Predicted siRNA GTGCTAACGTCCGTCGTGAA/ Down 3.15 60123 191 Predicted siRNA TAGCTTAACCTTCGGGAGGG/ Down 1.9 60334 192 Predicted siRNA TGAGAAAGAAAGAGAAGGC Down 1.64 60750 TCA/193 Predicted siRNA TGATGTCCTTAGATGTTCTG Down 1.99 60803 GGC/194 Predicted siRNA CATGTGTTCTCAGGTCGCCC Down 2.55 55413 C/195 Table 4. Provided are siRNAs that were found downregulated in Plants Growing under Nitrogen Deficient Conditions versus Optimal conditions.

Example 2 Target Prediction Using Bioinformatics Tools

A high throughput screening was performed on microarrays loaded with miRNAs/siRNAs that were found to be differentially expressed under multiple stress and optimal environmental conditions and in different plant tissues. The initial trait-associated miRNAs are later validated by quantitative Real Time PCR (qRT-PCR).

Target prediction—orthologous genes to the genes of interest in maize and/or Arabidopsis are found through a proprietary tool that analyzes publicly available genomic as well as expression and gene annotation databases from multiple plant species. Homologous as well as orthologous protein and nucleotide sequences of target genes of the small RNA sequences of the invention, were found using BLAST having at least 70% identity on at least 60% of the entire master gene length, and are summarized in Tables 5-8 below. BLAST version used was Version 2.2.25+, Released March 2011, at default parameters as follows: For step 1 using BlastX to find the master homolog: Word size 3, Gap open 11, gap extend 1. For step 2 using BlastN to find orthologs from other organisms: Word size 28, Gap open 0, Gap extend 0, Reward (match score) 1, Penalty (mismatch score)−2.

TABLE 5 Target Genes of miRNAs Associated with Increased NUE (Table 1) p.p. Mir Hom. SEQ p.n. Mir Bind. NCBI ID SEQ ID name Pos. Accession Annotation/Organism Identity NO: NO: osa-  95-115 XP_002468137 hypothetical protein 1.00 311 515 miR2096- SORBIDRAFT_01g040220 3p [Sorghum bicolor] &gt; gi|241921991|gb|EER95135.1| hypothetical protein SORBIDRAFT_01g040220 [Sorghum bicolor] ACN26598 unknown [Zea mays] 0.95 312 516 NP_001148956 LOC100282576 [Zea mays] 0.95 313 517 &gt; gi|195623616|gb|ACG33638.1| CONSTANS interacting protein 4 [Zea mays] ACG37488 CONSTANS interacting 0.96 314 518 protein 4 [Zea mays] NP_001148721 CONSTANS interacting 0.96 315 519 protein 4 [Zea mays] &gt; gi|195621640|gb|ACG32650.1| CONSTANS interacting protein 4 [Zea mays] NP_001049637 Os03g0263800 [Oryza sativa 0.89 316 520 Japonica Group] &gt; gi|29893607|gb|AAP06861.1| unknown protein [Oryza sativa Japonica Group] &gt; gi|108707320|gb|ABF95115.1| S-ribonuclease binding protein SBP1, putative, expressed [Oryza sativa Japonica Group] &gt; gi|113548108|dbj|BAF11551.1| Os03g0263800 [Oryza sativa Japonica Group] &gt; gi|215692696|dbj|BAG88116.1| unnamed protein product [Oryza sativa Japonica Group] &gt; gi|215704492|dbj|BAG93926.1| unnamed protein product [Oryza sativa Japonica Group] &gt; gi|222624614|gb|EEE58746.1| hypothetical protein OsJ_10235 [Oryza sativa Japonica Group] EEC74912 hypothetical protein 0.88 317 OsI_10851 [Oryza sativa Indica Group] BAJ94154 predicted protein [Hordeum 0.85 318 521 vulgare subsp. vulgare] &gt; gi|326493392|dbj|BAJ85157.1| predicted protein [Hordeum vulgare subsp. vulgare] Predicted 292-314 NP_001169860 hypothetical protein 1.00 319 522 zma LOC100383754 [Zea mays] mir &gt; gi|224032063|gb|ACN35107.1| 49161 unknown [Zea mays] XP_002458357 hypothetical protein 0.88 320 523 SORBIDRAFT_03g031980 [Sorghum bicolor] &gt; gi|241930332|gb|EES03477.1| hypothetical protein SORBIDRAFT_03g031980 [Sorghum bicolor] 282-304 ACN34890 unknown [Zea mays] 1.00 321 524 EEC71328 hypothetical protein 0.74 322 OsI_03374 [Oryza sativa Indica Group] &gt; gi|222619103|gb|EEE55235.1| hypothetical protein OsJ_03112 [Oryza sativa Japonica Group] BAD81811 hypothetical protein [Oryza 0.74 323 525 sativa Japonica Group] BAJ90295 predicted protein [Hordeum 0.74 324 526 vulgare subsp. vulgare] 353-375 XP_002458357 hypothetical protein 1.00 325 527 SORBIDRAFT_03g031980 [Sorghum bicolor] &gt; gi|241930332|gb|EES03477.1| hypothetical protein SORBIDRAFT_03g031980 [Sorghum bicolor] NP_001169860 hypothetical protein 0.88 326 528 LOC100383754 [Zea mays] &gt; gi|224032063|gb|ACN35107.1| unknown [Zea mays] Predicted  982-1003 XP_002446326 hypothetical protein 1.00 327 529 zma SORBIDRAFT_06g014320 mir [Sorghum bicolor] 50460 &gt; gi|241937509|gb|EES10654.1| hypothetical protein SORBIDRAFT_06g014320 [Sorghum bicolor] NP_001169348 hypothetical protein 0.84 328 530 LOC100383215 [Zea mays] &gt; gi|224028855|gb|ACN33503.1| unknown [Zea mays] ACN25775 unknown [Zea mays] 0.75 329 531 426-447 NP_001136483 hypothetical protein 1.00 330 532 LOC100216597 [Zea mays] &gt; gi|194695886|gb|ACF82027.1| unknown [Zea mays] 295-316 ACG40990 RING-H2 finger protein 1.00 331 533 ATL5I [Zea mays] NP_001159129 hypothetical protein 0.85 332 534 LOC100304207 [Zea mays] &gt; gi|223942155|gb|ACN25161.1| unknown [Zea mays] NP_001130949 hypothetical protein 0.77 333 535 LOC100192054 [Zea mays] &gt; gi|194690534|gb|ACF79351.1| unknown [Zea mays] BAJ92353 predicted protein [Hordeum 0.76 334 536 vulgare subsp. vulgare] NP_001057231 Os06g0233200 [Oryza sativa 0.76 335 537 Japonica Group] &gt; gi|51535192|dbj|BAD38165.1| putative RING finger 1 [Oryza sativa Japonica Group] &gt; gi|113595271|dbj|BAF19145.1| Os06g0233200 [Oryza sativa Japonica Group] &gt; gi|125554660|gb|EAZ00266.1| hypothetical protein OsI_22277 [Oryza sativa Indica Group] &gt; gi|215766038|dbj|BAG98266.1| unnamed protein product [Oryza sativa Japonica Group] &gt; gi|222635260|gb|EEE65392.1| hypothetical protein OsJ_20714 [Oryza sativa Japonica Group] 154-175 NP_001183617 hypothetical protein 1.00 336 538 LOC100502211 [Zea mays] &gt; gi|238013474|gb|ACR37772.1| unknown [Zea mays] XP_002465702 hypothetical protein 0.73 337 539 SORBIDRAFT_01g044080 [Sorghum bicolor] &gt; gi|241919556|gb|EER92700.1| hypothetical protein SORBIDRAFT_01g044080 [Sorghum bicolor]  93-114 NP_001152266 BHLH transcription factor 1.00 338 540 [Zea mays] &gt; gi|195654447|gb|ACG46691.1| BHLH transcription factor [Zea mays] 1159-1180 ACN25775 unknown [Zea mays] 1.00 339 541 NP_001169348 hypothetical protein 1.00 340 542 LOC100383215 [Zea mays] &gt; gi|224028855|gb|ACN33503.1| unknown [Zea mays] XP_002446326 hypothetical protein 0.80 341 543 SORBIDRAFT_06g014320 [Sorghum bicolor] &gt; gi|241937509|gb|EES10654.1| hypothetical protein SORBIDRAFT_06g014320 [Sorghum bicolor] Predicted 1984-2007 XP_002444807 hypothetical protein 1.00 342 544 folded SORBIDRAFT_07g028330 24-nts- [Sorghum bicolor] long &gt; gi|241941157|gb|EES14302.1| seq hypothetical protein 51695 SORBIDRAFT_07g028330 [Sorghum bicolor] NP_001169681 hypothetical protein 0.90 343 545 LOC100383562 [Zea mays] &gt; gi|224030801|gb|ACN34476.1| unknown [Zea mays] &gt; gi|224030845|gb|ACN34498.1| unknown [Zea mays] Predicted 584-606 NP_001161741 hypothetical protein 1.00 344 546 zma LOC100192929 isoform 3 mir [Zea mays] 48457 &gt; gi|226713359|sp|B6UGG4.1| NNJA4_MAIZE RecName: Full = Ninja-family protein 4 &gt; gi|195657959|gb|ACG48447.1| hypothetical protein [Zea mays] NP_001161740 hypothetical protein 1.00 345 547 LOC100192929 isoform 2 [Zea mays] &gt; gi|226713259|sp|B4FM28.1| NNJA2_MAIZE RecName: Full = Ninja-family protein 2 &gt; gi|194698174|gb|ACF83171.1| unknown [Zea mays] B6SLJ0 RecName: Full = Ninja-family 0.98 346 protein 3 &gt; gi|195607786|gb|ACG25723.1| hypothetical protein [Zea mays] ACN33721 unknown [Zea mays] 0.97 347 548 62-84 B6SLJ0 RecName: Full = Ninja-family 1.00 348 protein 3 &gt; gi|195607786|gb|ACG25723.1| hypothetical protein [Zea mays] NP_001161740 hypothetical protein 0.98 349 549 LOC100192929 isoform 2 [Zea mays] &gt; gi|226713259|sp|B4FM28.1| NNJA2_MAIZE RecName: Full = Ninja-family protein 2 &gt; gi|194698174|gb|ACF83171.1| unknown [Zea mays] ACN33721 unknown [Zea mays] 0.95 350 550 NP_001161741 hypothetical protein 0.97 351 551 LOC100192929 isoform 3 [Zea mays] &gt; gi|226713359|sp|B6UGG4.1| NNJA4_MAIZE RecName: Full = Ninja-family protein 4 &gt; gi|195657959|gb|ACG48447.1| hypothetical protein [Zea mays] 543-565 NP_001161740 hypothetical protein 1.00 352 552 LOC100192929 isoform 2 [Zea mays] &gt; gi|226713259|sp|B4FM28.1| NNJA2_MAIZE RecName: Full = Ninja-family protein 2 &gt; gi|194698174|gb|ACF83171.1| unknown [Zea mays] ACN33721 unknown [Zea mays] 0.97 353 553 NP_001161741 hypothetical protein 0.99 354 554 LOC100192929 isoform 3 [Zea mays] &gt; gi|226713359|sp|B6UGG4.1| NNJA4_MAIZE RecName: Full = Ninja-family protein 4 &gt; gi|195657959|gb|ACG48447.1| hypothetical protein [Zea mays] B6SLJ0 RecName: Full = Ninja-family 0.98 355 protein 3 &gt; gi|195607786|gb|ACG25723.1| hypothetical protein [Zea mays] 450-472 NP_001131584 hypothetical protein 1.00 356 555 LOC100192929 isoform 1 [Zea mays] &gt; gi|226713190|sp|B4FAF3.1| NNJA1_MAIZE RecName: Full = Ninja-family protein 1 &gt; gi|194690024|gb|ACF79096.1| unknown [Zea mays] &gt; gi|194691932|gb|ACF80050.1| unknown [Zea mays] &gt; gi|195638284|gb|ACG38610.1| hypothetical protein [Zea mays] XP_002465644 hypothetical protein 0.83 357 556 SORBIDRAFT_01g042880 [Sorghum bicolor] &gt; gi|241919498|gb|EER92642.1| hypothetical protein SORBIDRAFT_01g042880 [Sorghum bicolor] Predicted 1313-1334 NP_001182893 hypothetical protein 1.00 358 557 zma LOC100501172 [Zea mays] mir &gt; gi|238008020|gb|ACR35045.1| 50261 unknown [Zea mays] Predicted  86-109 XP_002447337 hypothetical protein 1.00 359 558 folded SORBIDRAFT_06g033160 24-nts- [Sorghum bicolor] long &gt; gi|241938520|gb|EES11665.1| seq hypothetical protein 52805 SORBIDRAFT_06g033160 [Sorghum bicolor] NP_001142056 hypothetical protein 0.96 360 559 LOC100274212 [Zea mays] &gt; gi|194706940|gb|ACF87554.1| unknown [Zea mays] &gt; gi|223947485|gb|ACN27826.1| unknown [Zea mays] ACG45259 hypothetical protein [Zea 0.96 361 560 mays] EEC78262 hypothetical protein 0.86 362 OsI_17948 [Oryza sativa Indica Group] EEE61915 hypothetical protein 0.86 363 OsJ_16648 [Oryza sativa Japonica Group] BAJ96591 predicted protein [Hordeum 0.86 364 561 vulgare subsp. vulgare] CAJ86266 H0901F07.3 [Oryza sativa 0.76 365 562 Indica Group] 596-619 AAY57857 cysteine proteinase inhibitor 1.00 366 563 [Zea mays subsp. parviglumis] &gt; gi|66866419|gb|AAY57858.1| cysteine proteinase inhibitor [Zea mays subsp. parviglumis] &gt; gi|66866423|gb|AAY57860.1| cysteine proteinase inhibitor [Zea mays subsp. parviglumis] &gt; gi|66866425|gb|AAY57861.1| cysteine proteinase inhibitor [Zea mays subsp. parviglumis] &gt; gi|66866427|gb|AAY57862.1| cysteine proteinase inhibitor [Zea mays subsp. parviglumis] &gt; gi|66866429|gb|AAY57863.1| cysteine proteinase inhibitor [Zea mays subsp. parviglumis] &gt; gi|66866435|gb|AAY57866.1| cysteine proteinase inhibitor [Zea mays subsp. parviglumis] AAY57867 cysteine proteinase inhibitor 0.99 367 564 [Zea mays subsp. parviglumis] ABQ32295 cysteine protease inhibitor 0.99 368 565 [Zea mays] AAY57864 cysteine proteinase inhibitor 0.96 369 566 [Zea mays subsp. parviglumis] &gt; gi|66866439|gb|AAY57868.1| cysteine proteinase inhibitor [Zea mays subsp. parviglumis] AAY57859 cysteine proteinase inhibitor 0.98 370 567 [Zea mays subsp. parviglumis] BAA09666 cysteine proteinase inhibitor 0.95 371 568 [Zea mays] &gt; gi|66866433|gb|AAY57865.1| cysteine proteinase inhibitor [Zea mays subsp. parviglumis] &gt; gi|71794635|emb|CAJ20024.1| putative cystatin [Zea mays] CAA60610 cysteine proteinase inhibitor 0.94 372 569 [Zea mays] NP_001106013 cystatin2 [Zea mays] 0.94 373 570 &gt; gi|1008922|dbj|BAA07327.1| cystatin II [Zea mays] BAB21558 cystatin [Coix lacryma-jobi] 0.86 374 571 NP_001105295 cystatin-1 precursor [Zea 0.84 375 572 mays] &gt; gi|399334|sp|P31726.1|CYT1_MAIZE RecName: Full = Cystatin-1; AltName: Full = Corn kernel cysteine proteinase inhibitor; AltName: Full = Cystatin I; Flags: Precursor &gt; gi|217962|dbj|BAA01472.1| corn cystatin I [Zea mays] 2324-2347 BAJ85758 predicted protein [Hordeum 1.00 376 573 vulgare subsp. vulgare] NP_001044791 Os01g0846600 [Oryza sativa 0.81 377 574 Japonica Group] &gt; gi|15408875|dbj|BAB64266.1| ankyrin-like protein [Oryza sativa Japonica Group] &gt; gi|20160625|dbj|BAB89571.1| ankyrin-like protein [Oryza sativa Japonica Group] &gt; gi|113534322|dbj|BAF06705.1| Os01g0846600 [Oryza sativa Japonica Group] &gt; gi|215687255|dbj|BAG91820.1| unnamed protein product [Oryza sativa Japonica Group] &gt; gi|222619533|gb|EEE55665.1| hypothetical protein OsJ_04065 [Oryza sativa Japonica Group] XP_002458754 hypothetical protein 0.81 378 575 SORBIDRAFT_03g039680 [Sorghum bicolor] &gt; gi|241930729|gb|EES03874.1| hypothetical protein SORBIDRAFT_03g039680 [Sorghum bicolor] ACR35053 unknown [Zea mays] 0.81 379 576 NP_001147927 LOC100281537 [Zea mays] 0.79 380 577 &gt; gi|195614640|gb|ACG29150.1| ankyrin-like protein [Zea mays] Predicted 1320-1343 XP_002457719 hypothetical protein 1.00 381 578 folded SORBIDRAFT_03g012350 24-nts- [Sorghum bicolor] long &gt; gi|241929694|gb|EES02839.1| seq hypothetical protein 51469 SORBIDRAFT_03g012350 [Sorghum bicolor] NP_001183022 hypothetical protein 0.85 382 579 LOC100501344 [Zea mays] &gt; gi|238008824|gb|ACR35447.1| unknown [Zea mays] XP_002457721 hypothetical protein 0.77 383 580 SORBIDRAFT_03g012360 [Sorghum bicolor] &gt; gi|241929696|gb|EES02841.1| hypothetical protein SORBIDRAFT_03g012360 [Sorghum bicolor] XP_002457722 hypothetical protein 0.73 384 581 SORBIDRAFT_03g012370 [Sorghum bicolor] &gt; gi|241929697|gb|EES02842.1| hypothetical protein SORBIDRAFT_03g012370 [Sorghum bicolor] zma- 158-179 NP_001147885 LOC100281495 [Zea mays] 1.00 385 582 miR399f* &gt; gi|195611982|gb|ACG27821.1| citrate transporter family protein [Zea mays] &gt; gi|195614372|gb|ACG29016.1| citrate transporter family protein [Zea mays] ACF86945 unknown [Zea mays] 0.93 386 583 ACG28034 citrate transporter family 0.98 387 584 protein [Zea mays] NP_001048962 Os03g0147400 [Oryza sativa 0.80 388 585 Japonica Group] &gt; gi|15451603|gb|AAK98727.1| AC090485_6 Putative anion transporter [Oryza sativa Japonica Group] &gt; gi|108706182|gb|ABF93977.1| transmembrane protein, putative, expressed [Oryza sativa Japonica Group] &gt; gi|108706183|gb|ABF93978.1| transmembrane protein, putative, expressed [Oryza sativa Japonica Group] &gt; gi|108706184|gb|ABF93979.1| transmembrane protein, putative, expressed [Oryza sativa Japonica Group] &gt; gi|108706185|gb|ABF93980.1| transmembrane protein, putative, expressed [Oryza sativa Japonica Group] &gt; gi|13547433|dbj|BAF10876.1| Os03g0147400 [Oryza sativa Japonica Group] &gt; gi|125542408|gb|EAY88547.1| hypothetical protein OsI_10021 [Oryza sativa Indica Group] &gt; gi|125584918|gb|EAZ25582.1| hypothetical protein OsJ_09409 [Oryza sativa Japonica Group] EAY78724 hypothetical protein 0.83 389 OsI_33828 [Oryza sativa Indica Group] &gt; gi|125574971|gb|EAZ16255.1| hypothetical protein OsJ_31712 [Oryza sativa Japonica Group] NP_001151517 LOC100285151 [Zea mays] 0.80 390 586 &gt; gi|195647360|gb|ACG43148.1| citrate transporter family protein [Zea mays] &gt; gi|223948219|gb|ACN28193.1| unknown [Zea mays] &gt; gi|223974939|gb|ACN31657.1| unknown [Zea mays] &gt; gi|238009364|gb|ACR35717.1| unknown [Zea mays] ACG43196 citrate transporter family 0.80 391 587 protein [Zea mays] ACL54556 unknown [Zea mays] 0.77 392 588 BAK05230 predicted protein [Hordeum 0.78 393 589 vulgare subsp. vulgare] BAK06146 predicted protein [Hordeum 0.78 394 590 vulgare subsp. vulgare] 650-671 XP_002460963 hypothetical protein 1.00 395 591 SORBIDRAFT_02g038300 [Sorghum bicolor] &gt; gi|241924340|gb|EER97484.1| hypothetical protein SORBIDRAFT_02g038300 [Sorghum bicolor] NP_001148467 LOC100282082 [Zea mays] 0.92 396 592 &gt; gi|195619570|gb|ACG31615.1| saccharopine dehydrogenase [Zea mays] EAZ04584 hypothetical protein 0.79 397 OsI_26734 [Oryza sativa Indica Group] BAJ97022 predicted protein [Hordeum 0.79 398 593 vulgare subsp. vulgare] EAZ40532 hypothetical protein 0.73 399 OsJ_24988 [Oryza sativa Japonica Group] Predicted 2327-2350 NP_001169325 hypothetical protein 1.00 400 594 folded LOC100383191 [Zea mays] 24-nts- &gt; gi|224028683|gb|ACN33417.1| long unknown [Zea mays] seq 52499 XP_002447125 hypothetical protein 0.98 401 595 SORBIDRAFT_06g029090 [Sorghum bicolor] &gt; gi|241938308|gb|EES11453.1| hypothetical protein SORBIDRAFT_06g029090 [Sorghum bicolor] Predicted 287-306 XP_002447878 hypothetical protein 1.00 402 596 zma SORBIDRAFT_06g017360 mir [Sorghum bicolor] 50545 &gt; gi|241939061|gb|EES12206.1| hypothetical protein SORBIDRAFT_06g017360 [Sorghum bicolor] NP_001151469 translocon Tic40 [Zea mays] 0.90 403 597 &gt; gi|219887501|gb|ACL54125.1| unknown [Zea mays] ACG42966 translocon Tic40 [Zea mays] 0.90 404 598 NP_001149949 translocon Tic40 [Zea mays] 0.90 405 599 &gt; gi|195635683|gb|ACG37310.1| translocon Tic40 [Zea mays] NP_001052871 Os04g0439900 [Oryza sativa 0.81 406 600 Japonica Group] &gt; gi|113564442|dbj|BAF14785.1| Os04g0439900 [Oryza sativa Japonica Group] &gt; gi|116309806|emb|CAH66845.1| H0525C06.8 [Oryza sativa Indica Group] &gt; gi|215704350|dbj|BAG93784.1| unnamed protein product [Oryza sativa Japonica Group] &gt; gi|218194904|gb|EEC77331.1| hypothetical protein OsI_16005 [Oryza sativa Indica Group] &gt; gi|222628923|gb|EEE61055.1| hypothetical protein OsJ_14912 [Oryza sativa Japonica Group] BAJ97578 predicted protein [Hordeum 0.78 407 601 vulgare subsp. vulgare] 46-65 NP_001182915 hypothetical protein 1.00 408 602 LOC100501201 [Zea mays] &gt; gi|238008164|gb|ACR35117.1| unknown [Zea mays] ACN33347 unknown [Zea mays] 0.84 409 603 NP_001150079 CID11 [Zea mays] 0.83 410 604 &gt; gi|195636508|gb|ACG37722.1| CID11 [Zea mays] XP_002444926 hypothetical protein 0.78 411 605 SORBIDRAFT_07g001560 [Sorghum bicolor] &gt; gi|241941276|gb|EES14421.1| hypothetical protein SORBIDRAFT_07g001560 [Sorghum bicolor] BAD33089 putative RNA-binding protein 0.74 412 RBP37 [Oryza sativa Japonica Group] &gt; gi|182375457|dbj|BAG24017.1| RNA-binding protein [Oryza sativa Japonica Group] &gt; gi|215736921|dbj|BAG95850.1| unnamed protein product [Oryza sativa Japonica Group] &gt; gi|222639804|gb|EEE67936.1| hypothetical protein OsJ_25822 [Oryza sativa Japonica Group] EEC82815 hypothetical protein 0.72 413 OsI_27601 [Oryza sativa Indica Group] BAJ96189 predicted protein [Hordeum 0.75 414 606 vulgare subsp. vulgare] XP_002518926 RNA-binding protein, 0.75 415 607 putative [Ricinus communis] &gt; gi|223541913|gb|EEF43459.1| RNA-binding protein, putative [Ricinus communis] XP_002272223 PREDICTED: hypothetical 0.75 416 608 protein [Vitis vinifera] &gt; gi|302143972|emb|CBI23077.3| unnamed protein product [Vitis vinifera] 605-624 NP_001142867 hypothetical protein 1.00 417 609 LOC100275266 [Zea mays] &gt; gi|195610696|gb|ACG27178.1| hypothetical protein [Zea mays] XP_002448750 hypothetical protein 0.83 418 610 SORBIDRAFT_06g032540 [Sorghum bicolor] &gt; gi|241939933|gb|EES13078.1| hypothetical protein SORBIDRAFT_06g032540 [Sorghum bicolor] NP_001143447 hypothetical protein 0.78 419 611 LOC100276102 [Zea mays] &gt; gi|195620654|gb|ACG32157.1| hypothetical protein [Zea mays] NP_001054225 Os04g0672300 [Oryza sativa 0.72 420 612 Japonica Group] &gt; gi|90265231|emb|CAH67766.1| H0322F07.3 [Oryza sativa Indica Group] &gt; gi|113565796|dbj|BAF16139.1| Os04g0672300 [Oryza sativa Japonica Group] &gt; gi|215715224|dbj|BAG94975.1| unnamed protein product [Oryza sativa Japonica Group] 360-379 NP_001043014 Os01g0358300 [Oryza sativa 1.00 421 613 Japonica Group] &gt; gi|53791615|dbj|BAD52962.1| unknown protein [Oryza sativa Japonica Group] &gt; gi|113532545|dbj|BAF04928.1| Os01g0358300 [Oryza sativa Japonica Group] &gt; gi|215765732|dbj|BAG87429.1| unnamed protein product [Oryza sativa Japonica Group] &gt; gi|218188197|gb|EEC70624.1| hypothetical protein OsI_01883 [Oryza sativa Indica Group] &gt; gi|222618419|gb|EEE54551.1| hypothetical protein OsJ_01736 [Oryza sativa Japonica Group] ACF87799 unknown [Zea mays] 0.86 422 614 ACN30638 unknown [Zea mays] 0.85 423 615 BAJ94646 predicted protein [Hordeum 0.84 424 616 vulgare subsp. vulgare] 557-576 XP_002455496 hypothetical protein 1.00 425 617 SORBIDRAFT_03g011990 [Sorghum bicolor] &gt; gi|241927471|gb|EES00616.1| hypothetical protein SORBIDRAFT_03g011990 [Sorghum bicolor] XP_002457709 hypothetical protein 0.98 426 618 SORBIDRAFT_03g011980 [Sorghum bicolor] &gt; gi|241929684|gb|EES02829.1| hypothetical protein SORBIDRAFT_03g011980 [Sorghum bicolor] ACN26409 unknown [Zea mays] 0.93 427 619 ACN27987 unknown [Zea mays] 0.93 428 620 NP_001152255 peroxidase 1 [Zea mays] 0.93 429 621 &gt; gi|195654333|gb|ACG46634.1| peroxidase 1 precursor [Zea mays] EAY73614 hypothetical protein 0.81 430 OsI_01501 [Oryza sativa Indica Group] NP_001042800 Os01g0294700 [Oryza sativa 0.81 431 622 Japonica Group] &gt; gi|9909174|dbj|BAB12033.1| putative peroxidase [Oryza sativa Japonica Group] &gt; gi|55700889|tpe|CAH69254.1| TPA: class III peroxidase 11 precursor [Oryza sativa Japonica Group] &gt; gi|113532331|dbj|BAF04714.1| Os01g0294700 [Oryza sativa Japonica Group] &gt; gi|215697074|dbj|BAG91068.1| unnamed protein product [Oryza sativa Japonica Group] &gt; gi|215737508|dbj|BAG96638.1| unnamed protein product [Oryza sativa Japonica Group] &gt; gi|215740794|dbj|BAG96950.1| unnamed protein product [Oryza sativa Japonica Group] EEE54389 hypothetical protein 0.76 432 OsJ_01407 [Oryza sativa Japonica Group] 517-536 ACN27987 unknown [Zea mays] 1.00 433 623 NP_001152255 peroxidase 1 [Zea mays] 1.00 434 624 &gt; gi|195654333|gb|ACG46634.1| peroxidase 1 precursor [Zea mays] ACN26409 unknown [Zea mays] 0.94 435 625 XP_002457709 hypothetical protein 0.93 436 626 SORBIDRAFT_03g011980 [Sorghum bicolor] &gt; gi|241929684|gb|EES02829.1| hypothetical protein SORBIDRAFT_03g011980 [Sorghum bicolor] XP_002455496 hypothetical protein 0.93 437 627 SORBIDRAFT_03g011990 [Sorghum bicolor] &gt; gi|241927471|gb|EES00616.1| hypothetical protein SORBIDRAFT_03g011990 [Sorghum bicolor] EAY73614 hypothetical protein 0.81 438 OsI_01501 [Oryza sativa Indica Group] NP_001042800 Os01g0294700 [Oryza sativa 0.81 439 628 Japonica Group] &gt; gi|9909174|dbj|BAB12033.1| putative peroxidase [Oryza sativa Japonica Group] &gt; gi|55700889|tpe|CAH69254.1| TPA: class III peroxidase 11 precursor [Oryza sativa Japonica Group] &gt; gi|113532331|dbj|BAF04714.1| Os01g0294700 [Oryza sativa Japonica Group] &gt; gi|215697074|dbj|BAG91068.1| unnamed protein product [Oryza sativa Japonica Group] &gt; gi|215737508|dbj|BAG96638.1| unnamed protein product [Oryza sativa Japonica Group] &gt; gi|215740794|dbj|BAG96950.1| unnamed protein product [Oryza sativa Japonica Group] EEE54389 hypothetical protein 0.76 440 OsJ_01407 [Oryza sativa Japonica Group] 286-305 ACG31680 pepsin A [Zea mays] 1.00 441 629 NP_001141522 hypothetical protein 0.99 442 630 LOC100273634 [Zea mays] &gt; gi|194704920|gb|ACF86544.1| unknown [Zea mays] &gt; gi|223949445|gb|ACN28806.1| unknown [Zea mays] ACL54367 unknown [Zea mays] 0.99 443 631 NP_001132197 hypothetical protein 0.89 444 632 LOC100193625 [Zea mays] &gt; gi|194693730|gb|ACF80949.1| unknown [Zea mays] &gt; gi|195605492|gb|ACG24576.1| pepsin A [Zea mays] NP_001048134 Os02g0751100 [Oryza sativa 0.80 445 633 Japonica Group] &gt; gi|46390211|dbj|BAD15642.1| aspartyl protease-like [Oryza sativa Japonica Group] &gt; gi|113537665|dbj|BAF10048.1| Os02g0751100 [Oryza sativa Japonica Group] &gt; gi|222623681|gb|EEE57813.1| hypothetical protein OsJ_08401 [Oryza sativa Japonica Group] EEC74016 hypothetical protein 0.79 446 OsI_08957 [Oryza sativa Indica Group] ACN27411 unknown [Zea mays] 0.77 447 634 BAK05106 predicted protein [Hordeum 0.75 448 635 vulgare subsp. vulgare] 309-328 NP_001143133 hypothetical protein 1.00 449 636 LOC100275611 [Zea mays] &gt; gi|195614826|gb|ACG29243.1| hypothetical protein [Zea mays] ACF83002 unknown [Zea mays] 0.99 450 637 BAJ87435 predicted protein [Hordeum 0.72 451 638 vulgare subsp. vulgare] 46-65 XP_002444926 hypothetical protein 1.00 452 639 SORBIDRAFT_07g001560 [Sorghum bicolor] &gt; gi|241941276|gb|EES14421.1| hypothetical protein SORBIDRAFT_07g001560 [Sorghum bicolor] ACN33347 unknown [Zea mays] 0.85 453 640 NP_001182915 hypothetical protein 0.87 454 641 LOC100501201 [Zea mays] &gt; gi|238008164|gb|ACR35117.1| unknown [Zea mays] NP_001150079 CID11 [Zea mays] 0.86 455 642 &gt; gi|195636508|gb|ACG37722.1| CID11 [Zea mays] BAD33089 putative RNA-binding protein 0.75 456 RBP37 [Oryza sativa Japonica Group] &gt; gi|182375457|dbj|BAG24017.1| RNA-binding protein [Oryza sativa Japonica Group] &gt; gi|215736921|dbj|BAG95850.1| unnamed protein product [Oryza sativa Japonica Group] &gt; gi|222639804|gb|EEE67936.1| hypothetical protein OsJ_25822 [Oryza sativa Japonica Group] EEC82815 hypothetical protein 0.72 457 OsI_27601 [Oryza sativa Indica Group] 40563 NP_001059821 Os07g0524100 [Oryza sativa 1.00 458 643 Japonica Group] &gt; gi|75118816|sp|Q69SA9.1| PDI54_ORYSJ RecName: Full = Protein disulfide isomerase-like 5-4; Short = OsPDIL5-4; AltName: Full = Protein disulfide isomerase-like 8-1; Short = OsPDIL8-1; Flags: Precursor &gt; gi|50508559|dbj|BAD30858.1| thioredoxin family-like protein [Oryza sativa Japonica Group] &gt; gi|113611357|dbj|BAF21735.1| Os07g0524100 [Oryza sativa Japonica Group] &gt; gi|215704615|dbj|BAG94243.1| unnamed protein product [Oryza sativa Japonica Group] &gt; gi|218199742|gb|EEC82169.1| hypothetical protein OsI_26259 [Oryza sativa Indica Group] &gt; gi|222637167|gb|EEE67299.1| hypothetical protein OsJ_24505 [Oryza sativa Japonica Group] BAJ86285 predicted protein [Hordeum 0.93 459 644 vulgare subsp. vulgare] CBG91903 putative PDI-like protein 0.93 460 [Triticum aestivum] &gt; gi|299469398|emb|CBG91917.1| putative PDI-like protein [Triticum aestivum] ACG39185 PDIL5-4-Zea mays protein 0.91 461 645 disulfide isomerase NP_001105762 protein disulfide isomerase12 0.91 462 646 [Zea mays] &gt; gi|59861281|gb|AAX09970.1| protein disulfide isomerase [Zea mays] ACN34146 unknown [Zea mays] 0.91 463 647 XP_002522864 thioredoxin domain- 0.76 464 648 containing protein, putative [Ricinus communis] &gt; gi|223537948|gb|EEF39562.1| thioredoxin domain- containing protein, putative [Ricinus communis] XP_002281649 PREDICTED: hypothetical 0.74 465 649 protein [Vitis vinifera] &gt; gi|297735969|emb|CBI23943.3| unnamed protein product [Vitis vinifera] XP_002319814 predicted protein [Populus 0.74 466 650 trichocarpa] &gt; gi|222858190|gb|EEE95737.1| predicted protein [Populus trichocarpa] XP_002317580 predicted protein [Populus 0.74 467 651 trichocarpa] &gt; gi|222860645|gb|EEE98192.1| predicted protein [Populus trichocarpa] 301-320 ACF87799 unknown [Zea mays] 1.00 468 652 ACN30638 unknown [Zea mays] 0.97 469 653 NP_001043014 Os01g0358300 [Oryza sativa 0.87 470 654 Japonica Group] &gt; gi|53791615|dbj|BAD52962.1| unknown protein [Oryza sativa Japonica Group] &gt; gi|113532545|dbj|BAF04928.1| Os01g0358300 [Oryza sativa Japonica Group] &gt; gi|215765732|dbj|BAG87429.1| unnamed protein product [Oryza sativa Japonica Group] &gt; gi|218188197|gb|EEC70624.1| hypothetical protein OsI_01883 [Oryza sativa Indica Group] &gt; gi|222618419|gb|EEE54551.1| hypothetical protein OsJ_01736 [Oryza sativa Japonica Group] BAJ94646 predicted protein [Hordeum 0.83 471 655 vulgare subsp. vulgare] Predicted 40752 ACF86440 unknown [Zea mays] 1.00 472 656 zma mir 48350 22-43 ABF67946 putative Opie4 pol protein 1.00 473 [Zea mays] AAC49502 Pol [Zea mays] 0.97 474 657 ABF67947 Opie2 pol protein [Zea mays] 0.96 475 ABF67934 Opie3 pol polyprotein [Zea 0.96 476 mays] AAL35396 Opie2a pol [Zea mays] 0.92 477 &gt; gi|168251075|gb|ACA21858.1| Opie2a pol protein [Zea mays] ABF67921 Ji1 putative pol protein [Zea 0.79 478 mays] AAD20307 copia-type pol polyprotein 0.79 479 [Zea mays] Predicted 810-831 XP_002455047 hypothetical protein 1.00 480 658 zma SORBIDRAFT_03g003530 mir [Sorghum bicolor] 49162 &gt; gi|241927022|gb|EES00167.1| hypothetical protein SORBIDRAFT_03g003530 [Sorghum bicolor] AAK51797 small heat shock protein 0.83 481 659 HSP17.8 [Triticum aestivum] BAJ86365 predicted protein [Hordeum 0.82 482 660 vulgare subsp. vulgare] NP_001105954 17.8 kDa class II heat shock 0.87 483 661 protein [Zea mays] &gt; gi|123553|sp|P24632.1|HSP22_MAIZE RecName: Full = 17.8 kDa class II heat shock protein &gt; gi|22337|emb|CAA38012.1| 18 kDa heat shock protein [Zea mays] &gt; gi|238009918|gb|ACR35994.1| unknown [Zea mays] &gt; gi|238015198|gb|ACR38634.1| unknown [Zea mays] CAI96500 17.6 kDa heat-shock protein 0.83 484 662 [Triticum turgidum subsp. dicoccon] BAK07165 predicted protein [Hordeum 0.81 485 663 vulgare subsp. vulgare] CAI96499 17.5 kDa heat-shock protein 0.81 486 664 [Triticum turgidum subsp. dicoccoides] CAI96501 17.6 kDa heat-shock protein 0.82 487 665 [Triticum turgidum subsp. durum] NP_001148454 17.5 kDa class II heat shock 0.87 488 666 protein [Zea mays] &gt; gi|195619384|gb|ACG31522.1| 17.5 kDa class II heat shock protein [Zea mays] BAK05681 predicted protein [Hordeum 0.80 489 667 vulgare subsp. vulgare] Predicted 597-618 XP_002456299 hypothetical protein 1.00 490 668 zma SORBIDRAFT_03g033710 mir [Sorghum bicolor] 48877 &gt; gi|241928274|gb|EES01419.1| hypothetical protein SORBIDRAFT_03g033710 [Sorghum bicolor] ACF79056 unknown [Zea mays] 0.92 491 669 &gt; gi|194693496|gb|ACF80832.1| unknown [Zea mays] &gt; gi|195626284|gb|ACG34972.1| GTP-binding protein [Zea mays] &gt; gi|223946703|gb|ACN27435.1| unknown [Zea mays] NP_001140242 hypothetical protein 0.92 492 670 LOC100272283 [Zea mays] &gt; gi|194698666|gb|ACF8417.1| unknown [Zea mays] NP_001044154 Os01g0732200 [Oryza sativa 0.84 493 671 Japonica Group] &gt; gi|57899709|dbj|BAD87429.1| putative GTP-binding protein [Oryza sativa Japonica Group] &gt; gi|113533685|dbj|BAF06068.1| Os01g0732200 [Oryza sativa Japonica Group] &gt; gi|215695283|dbj|BAG90474.1| unnamed protein product [Oryza sativa Japonica Group] EEC71435 hypothetical protein 0.84 494 OsI_03633 [Oryza sativa Indica Group] &gt; gi|222619209|gb|EEE5541.1| hypothetical protein OsJ_03357 [Oryza sativa Japonica Group] 218-239 XP_002463058 hypothetical protein 1.00 495 672 SORBIDRAFT_02g036900 [Sorghum bicolor] &gt; gi|241926435|gb|EER99579.1| hypothetical protein SORBIDRAFT_02g036900 [Sorghum bicolor] NP_001142066 hypothetical protein 0.90 496 673 LOC100274223 [Zea mays] &gt; gi|194706978|gb|ACF87573.1| unknown [Zea mays] BAJ95349 predicted protein [Hordeum 0.75 497 674 vulgare subsp. vulgare] &gt; gi|326526013|dbj|BAJ93183.1| predicted protein [Hordeum vulgare subsp. vulgare] EAZ40364 hypothetical protein 0.73 498 OsJ_24810 [Oryza sativa Japonica Group] EAZ04406 hypothetical protein 0.73 499 OsI_26550 [Oryza sativa Indica Group] Predicted 63-86 AAD11615 prpol [Zea mays] 1.00 500 675 folded 24-nts- long seq 52057 AAL76007 prpol [Zea mays] 0.97 501 907-930 AAD11616 prpol [Zea mays] 1.00 502 676 Predicted 387-410 XP_002445682 hypothetical protein 1.00 503 677 folded SORBIDRAFT_07g024170 24-nts- [Sorghum bicolor] long &gt; gi|241942032|gb|EES15177.1| seq hypothetical protein 52633 SORBIDRAFT_07g024170 [Sorghum bicolor] ACF83553 unknown [Zea mays] 0.90 504 678 &gt; gi|195646380|gb|ACG42658.1| glutathione S- transferase, N-terminal domain containing protein [Zea mays] ACN26529 unknown [Zea mays] 0.89 505 679 Predicted 450-470 NP_001141971 hypothetical protein 1.00 506 680 zma LOC100274121 [Zea mays] mir &gt; gi|194706630|gb|ACF87399.1| 48922 unknown [Zea mays] XP_002455881 hypothetical protein 0.79 507 681 SORBIDRAFT_03g026750 [Sorghum bicolor] &gt; gi|241927856|gb|EES01001.1| hypothetical protein SORBIDRAFT_03g026750 [Sorghum bicolor] Predicted 1046-1067 XP_002465053 hypothetical protein 1.00 508 682 zma SORBIDRAFT_01g031330 mir [Sorghum bicolor] 50077 &gt; gi|241918907|gb|EER92051.1| hypothetical protein SORBIDRAFT_01g031330 [Sorghum bicolor] NP_001137129 hypothetical protein 0.90 509 683 LOC100217311 [Zea mays] &gt; gi|194698486|gb|ACF83327.1| unknown [Zea mays] NP_001149312 fas-associated factor 1-like 0.93 510 684 protein [Zea mays] &gt; gi|195626306|gb|ACG34983.1| fas-associated factor 1- like protein [Zea mays] &gt; gi|223975789|gb|ACN32082.1| unknown [Zea mays] BAJ89712 predicted protein [Hordeum 0.83 511 685 vulgare subsp. vulgare] &gt; gi|326495414|dbj|BAJ85803.1| predicted protein [Hordeum vulgare subsp. vulgare] AAG13433 unknown protein [Oryza 0.85 512 686 sativa Japonica Group] &gt; gi|31433109|gb|AAP54662.1| UBX domain containing protein, expressed [Oryza sativa Japonica Group] &gt; gi|215678662|dbj|BAG92317.1| unnamed protein product [Oryza sativa Japonica Group] EAY79232 hypothetical protein 0.85 513 OsI_34349 [Oryza sativa Indica Group] EAZ16707 hypothetical protein 0.81 514 OsJ_32183 [Oryza sativa Japonica Group] Table 5: Provided are the target Genes of miRNAs Associated with Increased NUE (Table 1) along with their GenBank Accession numbers and sequence identifiers (SEQ ID NO:). “bind” = binding; “pos” = position; “hom” = homologue; “p.p.” = polypeptide; “p.n.” = polynucleotide.

TABLE 6 Target Genes of down regulated miRNAs Associated with Increased NUE (Table 2) Mir Hom. p.p. p.n. Mir Bind. NCBI SEQ ID SEQ name Pos. Accession Annotation/Organism Identity NO: ID NO: Predicted 743-763 NP_001148146 terpene synthase 7 [Zea mays] 1.00 687 1392 zma &gt; gi|195616112|gb|ACG29886.1| mir terpene synthase 7 [Zea 50318 mays] ACL54589 unknown [Zea mays] 0.99 688 1393 XP_002447434 hypothetical protein 0.71 689 1394 SORBIDRAFT_06g001020 [Sorghum bicolor] &gt; gi|241938617|gb|EES11762.1| hypothetical protein SORBIDRAFT_06g001020 [Sorghum bicolor] 776-796 XP_002455470 hypothetical protein 1.00 690 1395 SORBIDRAFT_03g011420 [Sorghum bicolor] &gt; gi|241927445|gb|EES00590.1| hypothetical protein SORBIDRAFT_03g011420 [Sorghum bicolor] NP_001151624 cyclin-like F-box [Zea mays] 0.90 691 1396 &gt; gi|195648178|gb|ACG43557.1| cyclin-like F-box [Zea mays] BAJ88045 predicted protein [Hordeum 0.79 692 1397 vulgare subsp. vulgare] &gt; gi|326521874|dbj|BAK04065.1| predicted protein [Hordeum vulgare subsp. vulgare] NP_001042756 Os01g0281000 [Oryza sativa 0.81 693 1398 Japonica Group] &gt; gi|6498442|dbj|BAA87845.1| unknown protein [Oryza sativa Japonica Group] &gt; gi|11041564|dbj|BAB00648.2| unnamed protein product [Oryza sativa Japonica Group] &gt; gi|11138071|dbj|BAB17744.1| OSJNBa0036E02.18 [Oryza sativa Japonica Group] &gt; gi|13873014|dbj|BAB44118.1| unknown protein [Oryza sativa Japonica Group] &gt; gi|113532287|dbj|BAF04670.1| Os01g0281000 [Oryza sativa Japonica Group] &gt; gi|215694300|dbj|BAG89293.1| unnamed protein product [Oryza sativa Japonica Group] EEE54342 hypothetical protein 0.75 694 OsJ_01321 [Oryza sativa Japonica Group] EEC70414 hypothetical protein 0.75 695 OsI_01411 [Oryza sativa Indica Group] 2058-2078 XP_002468633 hypothetical protein 1.00 696 1399 SORBIDRAFT_01g049370 [Sorghum bicolor] &gt; gi|241922487|gb|EER95631.1| hypothetical protein SORBIDRAFT_01g049370 [Sorghum bicolor] NP_001130361 hypothetical protein 0.98 697 1400 LOC100191456 [Zea mays] &gt; gi|194688936|gb|ACF78552.1| unknown [Zea mays] &gt; gi|194707734|gb|ACF87951.1| unknown [Zea mays] NP_001167830 hypothetical protein 0.96 698 1401 LOC100381530 [Zea mays] &gt; gi|223944309|gb|ACN26238.1| unknown [Zea mays] NP_001046725 Os02g0332200 [Oryza sativa 0.95 699 1402 Japonica Group] &gt; gi|46390985|dbj|BAD16520.1| putative cytosolic chaperonin delta-subunit [Oryza sativa Japonica Group] &gt; gi|113536256|dbj|BAF08639.1| Os02g0332200 [Oryza sativa Japonica Group] &gt; gi|215695341|dbj|BAG90532.1| unnamed protein product [Oryza sativa Japonica Group] &gt; gi|222636848|gb|EEE66980.1| hypothetical protein OsJ_23870 [Oryza sativa Japonica Group] EAZ06856 hypothetical protein 0.95 700 OsI_29091 [Oryza sativa Indica Group] BAJ97289 predicted protein [Hordeum 0.92 701 1403 vulgare subsp. vulgare] NP_001065054 Os10g0514600 [Oryza sativa 0.88 702 1404 Japonica Group] &gt; gi|10140686|gb|AAG13521.1| AC068924_26 putative cytosolic chaperonin, delta- subunit [Oryza sativa Japonica Group] &gt; gi|31433047|gb|AAP54607.1| T-complex protein 1, delta subunit, putative, expressed [Oryza sativa Japonica Group] &gt; gi|113639663|dbj|BAF26968.1| Os10g0514600 [Oryza sativa Japonica Group] &gt; gi|215687328|dbj|BAG91872.1| unnamed protein product [Oryza sativa Japonica Group] XP_002322076 predicted protein [Populus 0.81 703 1405 trichocarpa] &gt; gi|222869072|gb|EEF06203.1| predicted protein [Populus trichocarpa] XP_002317895 predicted protein [Populus 0.80 704 1406 trichocarpa] &gt; gi|222858568|gb|EEE96115.1| predicted protein [Populus trichocarpa] CAN70636 hypothetical protein 0.82 705 1407 VITISV_008621 [Vitis vinifera] 554-574 XP_002465983 hypothetical protein 1.00 706 1408 SORBIDRAFT_01g049510 [Sorghum bicolor] &gt; gi|241919837|gb|EER92981.1| hypothetical protein SORBIDRAFT_01g049510 [Sorghum bicolor] NP_001149716 fusca homolog [Zea mays] 0.95 707 1409 &gt; gi|195629716|gb|ACG36499.1| COP9 signalosome complex subunit 1 [Zea mays] NP_00148760 Os03g0116500 [Oryza sativa 0.89 708 1410 Japonica Group] &gt; gi|108705858|gb|ABF93653.1| COP9 signalosome complex subunit 1, putative, expressed [Oryza sativa Japonica Group] &gt; gi|113547231|dbj|BAF10674.1| Os03g0116500 [Oryza sativa Japonica Group] &gt; gi|125542143|gb|EAY88282.1| hypothetical protein OsI_09737 [Oryza sativa Indica Group] &gt; gi|125584695|gb|EAZ25359.1| hypothetical protein OsJ_09174 [Oryza sativa Japonica Group] &gt; gi|215678827|dbj|BAG95264.1| unnamed protein product [Oryza sativa Japonica Group] AAF40112 constitutive photomorphogenic 0.88 709 1411 11 [Oryza sativa Indica Group] AAG17476 rCOP11 protein [Oryza sativa 0.88 710 1412 Indica Group] BAJ99160 predicted protein [Hordeum 0.84 711 1413 vulgare subsp. vulgare] BAK01545 predicted protein [Hordeum 0.78 712 1414 vulgare subsp. vulgare] XP_002275895 PREDICTED: hypothetical 0.72 713 1415 protein [Vitis vinifera] &gt; gi|296089065|emb|CBI38768.3| unnamed protein product [Vitis vinifera] CAN74681 hypothetical protein 0.72 714 VITISV_025856 [Vitis vinifera] 372-392 XP_002448372 hypothetical protein 1.00 715 1416 SORBIDRAFT_06g026160 [Sorghum bicolor] &gt; gi|241939555|gb|EES12700.1| hypothetical protein SORBIDRAFT_06g026160 [Sorghum bicolor] ADZ96243 ACC synthase 1 [Saccharum 0.92 716 1417 hybrid cultivar SP80-3280] AAR25558 acc synthase [Zea mays] 0.92 717 1418 AAR25559 acc synthase [Zea mays] 0.91 718 1419 NP_001146401 1-aminocyclopropane-1- 0.91 719 1420 carboxylate synthase7 [Zea mays] &gt; gi|219886697|gb|ACL53723.1| unknown [Zea mays] ACN31344 unknown [Zea mays] 0.90 720 1421 BAJ95898 predicted protein [Hordeum 0.81 721 1422 vulgare subsp. vulgare] NP_001053637 Os04g0578000 [Oryza sativa 0.80 722 1423 Japonica Group] &gt; gi|32488497|emb|CAE03249.1| OSJNBa0011J08.4 [Oryza sativa Japonica Group] &gt; gi|113565208|dbj|BAF15551.1| Os04g0578000 [Oryza sativa Japonica Group] &gt; gi|215693849|dbj|BAG89048.1| unnamed protein product [Oryza sativa Japonica Group] CAH66732 H0404F02.8 [Oryza sativa 0.80 723 1424 Indica Group] &gt; gi|125549433|gb|EAY95255.1| hypothetical protein OsI_17075 [Oryza sativa Indica Group] AAB18416 ACC synthase [Triticum 0.80 724 1425 aestivum] 1204-1224 ACN26771 unknown [Zea mays] 1.00 725 1426 ACG31388 F-box domain containing 1.00 726 1427 protein [Zea mays] ACG45892 F-box domain containing 1.00 727 1428 protein [Zea mays] ACG25717 F-box domain containing 0.99 728 1429 protein [Zea mays] NP_001152119 F-box domain containing 0.96 729 1430 protein [Zea mays] &gt; gi|195652827|gb|ACG45881.1| F-box domain containing protein [Zea mays] XP_002467684 hypothetical protein 0.89 730 1431 SORBIDRAFT_01g032310 [Sorghum bicolor] &gt; gi|241921538|gb|EER94682.1| hypothetical protein SORBIDRAFT_01g032310 [Sorghum bicolor] EAY90508 hypothetical protein 0.82 731 OsI_12108 [Oryza sativa Indica Group] NP_001050391 Os03g0423000 [Oryza sativa 0.82 732 1432 Japonica Group] &gt; gi|30089733|gb|AAP20837.1| expressed protein [Oryza sativa Japonica Group] &gt; gi|108708889|gb|ABF96684.1| F-box domain containing protein, expressed [Oryza sativa Japonica Group] &gt; gi|113548862|dbj|BAF12305.1| Os03g0423000 [Oryza sativa Japonica Group] &gt; gi|125586708|gb|EAZ27372.1| hypothetical protein OsJ_11320 [Oryza sativa Japonica Group] &gt; gi|215697584|dbj|BAG91578.1| unnamed protein product [Oryza sativa Japonica Group] 1019-1039 XP_002464111 hypothetical protein 1.00 733 1433 SORBIDRAFT_01g012470 [Sorghum bicolor] &gt; gi|241917965|gb|EER91109.1| hypothetical protein SORBIDRAFT_01g012470 [Sorghum bicolor] NP_001140844 hypothetical protein 0.97 734 1434 LOC100272920 [Zea mays] &gt; gi|194701412|gb|ACF84790.1| unknown [Zea mays] &gt; gi|223943945|gb|ACN26056.1| unknown [Zea mays] NP_001149791 transmembrane protein 56 0.94 735 1435 [Zea mays] &gt; gi|195634693|gb|ACG36815.1| transmembrane protein 56 [Zea mays] NP_001050848 Os03g0666700 [Oryza sativa 0.90 736 1436 Japonica Group] &gt; gi|40538924|gb|AAR87181.1| expressed protein [Oryza sativa Japonica Group] &gt; gi|108710279|gb|ABF98074.1| expressed protein [Oryza sativa Japonica Group] &gt; gi|113549319|dbj|BAF12762.1| Os03g0666700 [Oryza sativa Japonica Group] &gt; gi|215692429|dbj|BAG87849.1| unnamed protein product [Oryza sativa Japonica Group] &gt; gi|215692647|dbj|BAG88067.1| unnamed protein product [Oryza sativa Japonica Group] &gt; gi|215716974|dbj|BAG95337.1| unnamed protein product [Oryza sativa Japonica Group] BAJ94278 predicted protein [Hordeum 0.91 737 1437 vulgare subsp. vulgare] &gt; gi|326505624|dbj|BAJ95483.1| predicted protein [Hordeum vulgare subsp. vulgare] &gt; gi|326513826|dbj|BAJ87931.1| predicted protein [Hordeum vulgare subsp. vulgare] &gt; gi|326518414|dbj|BAJ88236.1| predicted protein [Hordeum vulgare subsp. vulgare] ACF84098 unknown [Zea mays] 0.93 738 1438 EEE53990 hypothetical protein 0.90 739 OsJ_00621 [Oryza sativa Japonica Group] 1917-1937 XP_002453927 hypothetical protein 1.00 740 1439 SORBIDRAFT_04g021570 [Sorghum bicolor] &gt; gi|241933758|gb|EES06903.1| hypothetical protein SORBIDRAFT_04g021570 [Sorghum bicolor] NP_001105849 putative glycosyltransferase 0.96 741 1440 [Zea mays] &gt; gi|84794314|emb|CAJ57382.1| putative glycosyltransferase [Zea mays] BAK03947 predicted protein [Hordeum 0.86 742 1441 vulgare subsp. vulgare] NP_001047014 Os02g0529600 [Oryza sativa 0.86 743 1442 Japonica Group] &gt; gi|49388322|dbj|BAD25434.1| putative galactomannan galactosyltransferase [Oryza sativa Japonica Group] &gt; gi|113536545|dbj|BAF08928.1| Os02g0529600 [Oryza sativa Japonica Group] &gt; gi|215704838|dbj|BAG94866.1| unnamed protein product [Oryza sativa Japonica Group] &gt; gi|218190881|gb|EEC73308.1| hypothetical protein OsI_07488 [Oryza sativa Indica Group] &gt; gi|222622984|gb|EEE57116.1| hypothetical protein OsJ_06989 [Oryza sativa Japonica Group] 1590-1610 NP_001131407 hypothetical protein 1.00 744 1443 LOC100192736 [Zea mays] &gt; gi|194691442|gb|ACF79805.1 unknown [Zea mays] NP_001141840 hypothetical protein 0.78 745 1444 LOC100273982 [Zea mays] &gt; gi|194706136|gb|ACF87152.1| unknown [Zea mays] 510-530 XP_002460574 hypothetical protein 1.00 746 1445 SORBIDRAFT_02g031070 [Sorghum bicolor] &gt; gi|241923951|gb|EER97095.1| hypothetical protein SORBIDRAFT_02g031070 [Sorghum bicolor] NP_001151754 collagen, type IV, alpha 5 [Zea 0.87 747 1446 mays] &gt; gi|195649521|gb|ACG44228.1 collagen, type IV, alpha 5 [Zea mays] 876-896 XP_002437709 hypothetical protein 1.00 748 1447 SORBIDRAFT_10g001120 [Sorghum bicolor] &gt; gi|241915932|gb|EER89076.1| hypothetical protein SORBIDRAFT_10g001120 [Sorghum bicolor] ACN34709 unknown [Zea mays] 0.99 749 1448 ACN28096 unknown [Zea mays] 0.99 750 1449 &gt; gi|223948483|gb|ACN28325.1| unknown [Zea mays] NP_001056601 Os06g0114000 [Oryza sativa 0.97 751 1450 Japonica Group] &gt; gi|7248401|dbj|BAA92724.1| putative chaperonin 60 beta precursor [Oryza sativa Japonica Group] &gt; gi|113594641|dbj|BAF18515.1| Os06g0114000 [Oryza sativa Japonica Group] &gt; gi|164375533|gb|ABY52934.1| RuBisCo subunit binding-protein beta subunit [Oryza sativa Japonica Group] &gt; gi|215715264|dbj|BAG95015.1| unnamed protein product [Oryza sativa Japonica Group] EEE64974 hypothetical protein 0.93 752 OsJ_19883 [Oryza sativa Japonica Group] NP_001045601 Os02g0102900 [Oryza sativa 0.87 753 1451 Japonica Group] &gt; gi|41052909|dbj|BAD07821.1| putative RuBisCO subunit binding-protein beta subunit, chloroplast precursor [Oryza sativa Japonica Group] &gt; gi|113535132|dbj|BAF07515.1| Os02g0102900 [Oryza sativa Japonica Group] &gt; gi|218189867|gb|EEC72294.1| hypothetical protein OsI_05469 [Oryza sativa Indica Group] &gt; gi|222621999|gb|EEE56131.1| hypothetical protein OsJ_05004 [Oryza sativa Japonica Group] &gt; gi|313575775|gb|ADR66967.1| 60 kDa chaperonin beta subunit [Oryza sativa Japonica Group] XP_002285746 PREDICTED: hypothetical 0.88 754 1452 protein [Vitis vinifera] &gt; gi|297746501|emb|CBI16557.3| unnamed protein product [Vitis vinifera] XP_002303983 predicted protein [Populus 0.87 755 1453 trichocarpa] &gt; gi|222841415|gb|EEE78962.1| predicted protein [Populus trichocarpa] XP_002523404 rubisco subunit binding- 0.87 756 1454 protein beta subunit, rubb, putative [Ricinus communis] &gt; gi|223537354|gb|EEF38983.1| rubisco subunit binding- protein beta subunit, rubb, putative [Ricinus communis] XP_002894506 CPN60B [Arabidopsis lyrata 0.87 757 1455 subsp. lyrata] &gt; gi|297340348|gb|EFH70765.1| CPN60B [Arabidopsis lyrata subsp. lyrata] 640-660 XP_002456142 hypothetical protein 1.00 758 1456 SORBIDRAFT_03g031180 [Sorghum bicolor] &gt; gi|241928117|gb|EES01262.1| hypothetical protein SORBIDRAFT_03g031180 [Sorghum bicolor] NP_001148838 LOC100282456 [Zea mays] 0.94 759 1457 &gt; gi|195622536|gb|ACG33098.1| THAP domain- containing protein 4 [Zea mays] EEC71270 hypothetical protein 0.87 760 OsI_03268 [Oryza sativa Indica Group] CBH32601 conserved hypothetical 0.83 761 1458 protein, expressed [Triticum aestivum] NP_001043867 Os01g0679600 [Oryza sativa 0.80 762 1459 Japonica Group] &gt; gi|56202146|dbj|BAD73479.1| unknown protein [Oryza sativa Japonica Group] &gt; gi|255673554|dbj|BAF05781.2| Os01g0679600 [Oryza sativa Japonica Group] BAJ94714 predicted protein [Hordeum 0.78 763 1460 vulgare subsp. vulgare] BAD73478 unknown protein [Oryza sativa 0.78 764 1461 Japonica Group] &gt; gi|215693000|dbj|BAG88420.1| unnamed protein product [Oryza sativa Japonica Group] &gt; gi|222619045|gb|EEE55177.1| hypothetical protein OsJ_03010 [Oryza sativa Japonica Group] 1204-1224 ACR38511 unknown [Zea mays] 1.00 765 1462 ACG45146 hypothetical protein [Zea 1.00 766 1463 mays] XP_002440220 hypothetical protein 0.71 767 1464 SORBIDRAFT_09g027950 [Sorghum bicolor] &gt; gi|241945505|gb|EES18650.1| hypothetical protein SORBIDRAFT_09g027950 [Sorghum bicolor] NP_001144829 hypothetical protein 0.71 768 1465 LOC100277913 [Zea mays] &gt; gi|195647628|gb|ACG43282.1| hypothetical protein [Zea mays] 916-936 NP_001143951 hypothetical protein 1.00 769 1466 LOC100276764 [Zea mays] &gt; gi|195631554|gb|ACG36672.1| hypothetical protein [Zea mays] NP_001169519 hypothetical protein 0.81 770 1467 LOC100383393 [Zea mays] &gt; gi|224029837|gb|ACN33994.1| unknown [Zea mays] 1178-1198 XP_002468109 hypothetical protein 1.00 771 1468 SORBIDRAFT_01g039740 [Sorghum bicolor] &gt; gi|241921963|gb|EER95107.1| hypothetical protein SORBIDRAFT_01g039740 [Sorghum bicolor] NP_001131833 hypothetical protein 0.95 772 1469 LOC100193208 [Zea mays] &gt; gi|194688576|gb|ACF78372.1| unknown [Zea mays] &gt; gi|195627610|gb|ACG35635.1| protein binding protein [Zea mays] ACL53375 unknown [Zea mays] 0.94 773 1470 NP_001147045 protein binding protein [Zea 0.93 774 1471 mays] &gt; gi|195606842|gb|ACG25251.1| protein binding protein [Zea mays] ABF95226 zinc finger family protein, 0.86 775 putative, expressed [Oryza sativa Japonica Group] &gt; gi|108707432|gb|ABF95227.1| zinc finger family protein, putative, expressed [Oryza sativa Japonica Group] &gt; gi|218192524|gb|EEC74951.1| hypothetical protein OsI_10935 [Oryza sativa Indica Group] &gt; gi|222624650|gb|EEE58782.1| hypothetical protein OsJ_10309 [Oryza sativa Japonica Group] BAJ90599 predicted protein [Hordeum 0.81 776 1472 vulgare subsp. vulgare] ADX86798 zinc-finger transcription factor 0.79 777 1473 [Triticum aestivum] ACN35095 unknown [Zea mays] 0.70 778 1474 NP_001049696 Os03g0272300 [Oryza sativa 0.70 779 1475 Japonica Group] &gt; gi|108707430|gb|ABF95225.1| zinc finger family protein, putative, expressed [Oryza sativa Japonica Group] &gt; gi|215765669|dbj|BAG87366.1| unnamed protein product [Oryza sativa Japonica Group] &gt; gi|255674397|dbj|BAF11610.2| Os03g0272300 [Oryza sativa Japonica Group] 1068-1088 NP_001144727 hypothetical protein 1.00 780 1476 LOC100277769 [Zea mays] &gt; gi|195646260|gb|ACG42598.1| hypothetical protein [Zea mays] NP_001141817 hypothetical protein 0.99 781 1477 LOC100273956 [Zea mays] &gt; gi|194706038|gb|ACF87103.1| unknown [Zea mays] NP_001170260 hypothetical protein 0.96 782 1478 LOC100384217 [Zea mays] &gt; gi|224034675|gb|ACN36413.1| unknown [Zea mays] NP_001170241 hypothetical protein 0.71 783 1479 LOC100384195 [Zea mays] &gt; gi|224034547|gb|ACN36349.1| unknown [Zea mays] 573-593 XP_002454448 hypothetical protein 1.00 784 1480 SORBIDRAFT_04g031260 [Sorghum bicolor] &gt; gi|241934279|gb|EES07424.1| hypothetical protein SORBIDRAFT_04g031260 [Sorghum bicolor] NP_001149829 LOC100283456 [Zea mays] 0.97 785 1481 &gt; gi|195634913|gb|ACG36925.1| acetylornithine deacetylase [Zea mays] EEC73818 hypothetical protein 0.85 786 OsI_08537 [Oryza sativa Indica Group] NP_001047794 Os02g0690800 [Oryza sativa 0.85 787 1482 Japonica Group] &gt; gi|41053258|dbj|BAD07684.1| putative silverleaf whitefly-induced protein 1 [Oryza sativa Japonica Group] &gt; gi|113537325|dbj|BAF09708.1| Os02g0690800 [Oryza sativa Japonica Group] &gt; gi|215715374|dbj|BAG95125.1| unnamed protein product [Oryza sativa Japonica Group] &gt; gi|215740943|dbj|BAG97438.1| unnamed protein product [Oryza sativa Japonica Group] &gt; gi|222623478|gb|EEE57610.1| hypothetical protein OsJ_07995 [Oryza sativa Japonica Group] 1820-1840 XP_002456060 hypothetical protein 1.00 788 1483 SORBIDRAFT_03g029660 [Sorghum bicolor] &gt; gi|241928035|gb|EES01180.1| hypothetical protein SORBIDRAFT_03g029660 [Sorghum bicolor] ACG31412 wax synthase isoform 3 [Zea 0.86 789 1484 mays] NP_001150127 wax synthase [Zea mays] 0.86 790 1485 &gt; gi|195636988|gb|ACG37962.1| wax synthase isoform 3 [Zea mays] NP_001043733 Os01g0651500 [Oryza sativa 0.73 791 1486 Japonica Group] &gt; gi|20146429|dbj|BAB89209.1| wax synthase-like [Oryza sativa Japonica Group] &gt; gi|113533264|dbj|BAF05647.1| Os01g0651500 [Oryza sativa Japonica Group] &gt; gi|125571401|gb|EAZ12916.1| hypothetical protein OsJ_02839 [Oryza sativa Japonica Group] EAY75194 hypothetical protein 0.72 792 OsI_03086 [Oryza sativa Indica Group] BAK07830 predicted protein [Hordeum 0.70 793 1487 vulgare subsp. vulgare] 1709-1729 XP_002448959 hypothetical protein 1.00 794 1488 SORBIDRAFT_05g002450 [Sorghum bicolor] &gt; gi|241934802|gb|EES07947.1| hypothetical protein SORBIDRAFT_05g002450 [Sorghum bicolor] ACF84627 unknown [Zea mays] 0.95 795 1489 &gt; gi|223942887|gb|ACN25527.1| unknown [Zea mays] NP_001150994 PP2A regulatory subunit 0.95 796 1490 TAP46 [Zea mays] &gt; gi|195643460|gb|ACG41198.1| PP2A regulatory subunit TAP46 [Zea mays] ACF84366 unknown [Zea mays] 0.94 797 1491 EAY79895 hypothetical protein 0.89 798 OsI_35058 [Oryza sativa Indica Group] NP_001066109 Os12g0137500 [Oryza sativa 0.89 799 1492 Japonica Group] &gt; gi|77552970|gb|ABA95766.1| TAP42-like family protein, expressed [Oryza sativa Japonica Group] &gt; gi|113648616|dbj|BAF29128.1| Os12g0137500 [Oryza sativa Japonica Group] &gt; gi|2157690835|dbj|BAH01312.1| unnamed protein product [Oryza sativa Japonica Group] EAZ19586 hypothetical protein 0.89 800 OsJ_35163 [Oryza sativa Japonica Group] AAO72555 PP2A regulatory subunit-like 0.89 801 1493 protein [Oryza sativa Japonica Group] ABA91420 TAP42-like family protein, 0.88 802 1494 expressed [Oryza sativa Japonica Group] &gt; gi|222615497|gb|EEE51629.1| hypothetical protein OsJ_32912 [Oryza sativa Japonica Group] NP_001148561 PP2A regulatory subunit 0.86 803 1495 TAP46 [Zea mays] &gt; gi|195620440|gb|ACG32050.1| PP2A regulatory subunit TAP46 [Zea mays] 1417-1437 XP_002465624 hypothetical protein 1.00 804 1496 SORBIDRAFT_01g042450 [Sorghum bicolor] &gt; gi|194326198|emb|CAQ86599.1| glutamine synthetase [Sorghum bicolor] &gt; gi|241919478|gb|EER92622.1| hypothetical protein SORBIDRAFT_01g042450 [Sorghum bicolor] AAW21274 glutamine synthetase 0.98 805 1497 [Saccharum officinarum] NP_001105538 glutamine synthetase root 0.97 806 1498 isozyme 1 [Zea mays] &gt; gi|6996235|dbj|BAA03433.1| glutamine synthetase [Zea mays] &gt; gi|194701040|gb|ACF84604.1| unknown [Zea mays] P38559 RecName: Full = Glutamine 0.97 807 synthetase root isozyme 1; AltName: Full = GS122; AltName: Full = Glutamate-- ammonia ligase &gt; gi|434324|emb|CAA46719.1| glutamine synthetase [Zea mays] P38563 RecName: Full = Glutamine 0.96 808 synthetase root isozyme 5; AltName: Full = GS117; AltName: Full = Glutamate-- ammonia ligase NP_001105297 glutamine synthetase root 0.96 809 1499 isozyme 5 [Zea mays] &gt; gi|699621|dbj|BAA03432.1| glutamine synthetase [Zea mays] ACG47508 glutamine synthetase root 0.95 810 1500 isozyme 5 [Zea mays] NP_001049424 Os03g0223400 [Oryza sativa 0.91 811 1501 Japonica Group] &gt; gi|121332|sp|P14654.1|GLN12_ORYSJ RecName: Full = Glutamine synthetase cytosolic isozyme 1-2; AltName: Full = Glutamate-- ammonia ligase GLN1; 2; Short = OsGLN1; 2; AltName: Full = Glutamine synthetase root isozyme; AltName: Full = OsGS1; 2 &gt; gi|20358|emb|CAA32460.1| unnamed protein product [Oryza sativa Japonica Group] &gt; gi|565414215|dbj|BAD77931.1| cytosolic glutamine synthetase 1; 2 [Oryza sativa Japonica Group] &gt; gi|108706922|gb|ABF94717.1| Glutamine synthetase root isozyme 5, putative, expressed [Oryza sativa Japonica Group] &gt; gi|1135478955|dbj|BAF11338.1| Os03g0223400 [Oryza sativa Japonica Group] &gt; gi|215769188|dbj|BAH01417.1| unnamed protein product [Oryza sativa Japonica Group] &gt; gi|218192361|gb|EEC74788.1| hypothetical protein OsI_10575 [Oryza sativa Indica Group] &gt; gi|222624488|gb|EEE58620.1| hypothetical protein OsJ_09974 [Oryza sativa Japonica Group] AAR84347 glutamine synthetase isoform 0.89 812 1502 GSr1 [Triticum aestivum] AAR84348 glutamine synthetase isoform 0.89 813 1503 GSr2 [Triticum aestivum] 1527-1547 XP_002454761 hypothetical protein 1.00 814 1504 SORBIDRAFT_04g036890 [Sorghum bicolor] &gt; gi|241934592|gb|EES07737.1| hypothetical protein SORBIDRAFT_04g036890 [Sorghum bicolor] NP_001130621 hypothetical protein 0.87 815 1505 LOC100191720 [Zea mays] &gt; gi|194689664|gb|ACF78916.1| unknown [Zea mays] XP_002454762 hypothetical protein 0.78 816 1506 SORBIDRAFT_04g036900 [Sorghum bicolor] &gt; gi|241934593|gb|EES07738.1| hypothetical protein SORBIDRAFT_04g036900 [Sorghum bicolor] XP_002437453 hypothetical protein 0.70 817 1507 SORBIDRAFT_10g027360 [Sorghum bicolor] &gt; gi|241915676|gb|EER88820.1| hypothetical protein SORBIDRAFT_10g027360 [Sorghum bicolor] XP_002438878 hypothetical protein 0.69 818 1508 SORBIDRAFT_10g027650 [Sorghum bicolor] &gt; gi|241917101|gb|EER90245.1| hypothetical protein SORBIDRAFT_10g027650 [Sorghum bicolor] XP_002438877 hypothetical protein 0.70 819 1509 SORBIDRAFT_10g027640 [Sorghum bicolor] &gt; gi|241917100|gb|EER90244.1| hypothetical protein SORBIDRAFT_10g027640 [Sorghum bicolor] 2363-2383 XP_002436826 hypothetical protein 1.00 820 1510 SORBIDRAFT_10g009530 [Sorghum bicolor] &gt; gi|241915049|gb|EER88193.1| hypothetical protein SORBIDRAFT_10g009530 [Sorghum bicolor] ACN31818 unknown [Zea mays] 0.94 821 1511 BAJ98035 predicted protein [Hordeum 0.81 822 1512 vulgare subsp. vulgare] NP_001057335 Os06g0264500 [Oryza sativa 0.79 823 1513 Japonica Group] &gt; gi|53793159|dbj|BAD54367.1| putative nitrite transporter [Oryza sativa Japonica Group] &gt; gi|53793165|dbj|BAD54372.1| putative nitrite transporter [Oryza sativa Japonica Group] &gt; gi|113595375|dbj|BAF19249.1| Os06g0264500 [Oryza sativa Japonica Group] &gt; gi|215767857|dbj|BAH00086.1| unnamed protein product [Oryza sativa Japonica Group] &gt; gi|218197932|gb|EEC80359.1| hypothetical protein OsI_22454 [Oryza sativa Indica Group] &gt; gi|222635351|gb|EEE65483.1| hypothetical protein OsJ_20894 [Oryza sativa Japonica Group] 2309-2329 XP_002446671 hypothetical protein 1.00 824 1514 SORBIDRAFT_06g020190 [Sorghum bicolor] &gt; gi|241937854|gb|EES10999.1| hypothetical protein SORBIDRAFT_06g020190 [Sorghum bicolor] NP_001151527 ATSWI3A [Zea mays] 0.92 825 1515 &gt; gi|195647424|gb|ACG43180.1| ATSWI3A [Zea mays] NP_001053100 Os04g0480300 [Oryza sativa 0.76 826 1516 Japonica Group] &gt; gi|113564671|dbj|BAF15014.1| Os04g0480300 [Oryza sativa Japonica Group] &gt; gi|215734840|dbj|BAG95562.1| unnamed protein product [Oryza sativa Japonica Group] CAH66875 OSIGBa0158F13.6 [Oryza 0.76 827 1517 sativa Indica Group] EEC77490 hypothetical protein 0.73 828 OsI_16334 [Oryza sativa Indica Group] 1469-1489 NP_001148712 plant-specific domain 1.00 829 1518 TIGR01589 family protein [Zea mays] &gt; gi|195621580|gb|ACG32620.1| plant-specific domain TIGR01589 family protein [Zea mays] 2030-2050 NP_001105116 SBP-domain protein3 [Zea 1.00 830 1519 mays] &gt; gi|5931782|emb|CAB56629.1| SBP-domain protein 3 [Zea mays] XP_002437398 hypothetical protein 0.78 831 1520 SORBIDRAFT_10g026200 [Sorghum bicolor] &gt; gi|241915621|gb|EER88765.1| hypothetical protein SORBIDRAFT_10g026200 [Sorghum bicolor] ACF78942 unknown [Zea mays] 0.70 832 1521 &gt; gi|194690438|gb|ACF79303.1| unknown [Zea mays] &gt; gi|223974801|gb|ACN31588.1| unknown [Zea mays] NP_001105656 SBP-domain protein4 [Zea 0.69 833 1522 mays] &gt; gi|5931784|emb|CAB56630.1| SBP-domain protein 4 [Zea mays] 1925-1945 NP_001142263 hypothetical protein 1.00 834 1523 LOC100274432 [Zea mays] &gt; gi|194707898|gb|ACF88033.1| unknown [Zea mays] XP_002468497 hypothetical protein 0.81 835 1524 SORBIDRAFT_01g046950 [Sorghum bicolor] &gt; gi|241922351|gb|EER95495.1| hypothetical protein SORBIDRAFT_01g046950 [Sorghum bicolor]  88-108 XP_002448249 hypothetical protein 1.00 836 1525 SORBIDRAFT_06g023950 [Sorghum bicolor] &gt; gi|241939432|gb|EES12577.1| hypothetical protein SORBIDRAFT_06g023950 [Sorghum bicolor] NP_001141834 hypothetical protein 0.92 837 1526 LOC100273976 [Zea mays] &gt; gi|194706118|gb|ACF87143.1| unknown [Zea mays] BAJ88132 predicted protein [Hordeum 0.86 838 1527 vulgare subsp. vulgare] CAE02784 OSJNBa0011L07.8 [Oryza 0.87 839 1528 sativa Japonica Group] &gt; gi|116310379|emb|CAH67390.1| H0115B09.2 [Oryza sativa Indica Group] &gt; gi|215768681|dbj|BAH00910.1| unnamed protein product [Oryza sativa Japonica Group] &gt; gi|218195296|gb|EEC77723.1| hypothetical protein OsI_16815 [Oryza sativa Indica Group] &gt; gi|222629289|gb|EEE61421.1| hypothetical protein OsJ_15623 [Oryza sativa Japonica Group] ACF88110 unknown [Zea mays] 0.76 840 1529 NP_001065045 Os10g0512700 [Oryza sativa 0.73 841 1530 Japonica Group] &gt; gi|78708906|gb|ABB47881.1| SH3 domain-containing protein 3, putative, expressed [Oryza sativa Japonica Group] &gt; gi|113639654|dbj|BAF26959.1| Os10g0512700 [Oryza sativa Japonica Group] &gt; gi|218184876|gb|EEC67303.1| hypothetical protein OsI_34298 [Oryza sativa Indica Group] &gt; gi|222613131|gb|EEE51263.1| hypothetical protein OsJ_32141 [Oryza sativa Japonica Group] BAJ89635 predicted protein [Hordeum 0.72 842 1531 vulgare subsp. vulgare] ACG28140 clathrin binding protein [Zea 0.72 843 1532 mays] NP_001130175 hypothetical protein 0.72 844 1533 LOC100191269 [Zea mays] &gt; gi|194688470|gb|ACF78319.1| unknown [Zea mays] XP_002464381 hypothetical protein 0.71 845 1534 SORBIDRAFT_01g017250 [Sorghum bicolor] &gt; gi|241918235|gb|EER91379.1| hypothetical protein SORBIDRAFT_01g017250 [Sorghum bicolor] 2810-2830 XP_002458234 hypothetical protein 1.00 846 1535 SORBIDRAFT_03g029520 [Sorghum bicolor] &gt; gi|241930209|gb|EES03354.1| hypothetical protein SORBIDRAFT_03g029520 [Sorghum bicolor] CAI77627 potassium uptake channel [Zea 0.93 847 mays] NP_001105480 potassium channel5 [Zea 0.91 848 1536 mays] &gt; gi|2104908|emb|CAA68912.1| potassium channel [Zea mays] NP_001043713 Os01g0648000 [Oryza sativa 0.82 849 1537 Japonica Group] &gt; gi|122241153|sp|Q0JKV1.1| AKT1_ORYSJ RecName: Full = Potassium channel AKT1; Short = OsAKT1 &gt; gi|113533244|dbj|BAF05627.1| Os01g0648000 [Oryza sativa Japonica Group] P0C550 RecName: Full = Potassium 0.82 850 channel AKT1; Short = OsAKT1 ABE99810 inwardly rectifying potassium 0.81 851 1538 channel AKT1 [Hordeum vulgare] &gt; gi|326519172|dbj|BAJ96585.1| predicted protein [Hordeum vulgare subsp. vulgare] AAF36832 AKT1-like potassium channel 0.81 852 1539 [Triticum aestivum] AAL40894 AKT1-like potassium channel 0.78 853 1540 [Oryza sativa] ADK93728 inward-rectifying potassium 0.79 854 1541 channel [Puccinellia tenuiflora] EEE55080 hypothetical protein 0.78 855 OsJ_02815 [Oryza sativa Japonica Group] 1465-1485 NP_001151754 collagen, type IV, alpha 5 [Zea 1.00 856 1542 mays] &gt; gi|195649521|gb|ACG44228.1| collagen, type IV, alpha 5 [Zea mays] XP_002460574 hypothetical protein 0.87 857 1543 SORBIDRAFT_02g031070 [Sorghum bicolor] &gt; gi|241923951|gb|EER97095.1| hypothetical protein SORBIDRAFT_02g031070 [Sorghum bicolor] 1702-1722 XP_002460183 hypothetical protein 1.00 858 1544 SORBIDRAFT_02g024090 [Sorghum bicolor] &gt; gi|241923560|gb|EER96704.1| hypothetical protein SORBIDRAFT_02g024090 [Sorghum bicolor] NP_001149599 LOC100283225 [Zea mays] 0.96 859 1545 &gt; gi|194705944|gb|ACF87056.1| unknown [Zea mays] &gt; gi|195628360|gb|ACG36010.1| annexin A4 [Zea mays] ACG40326 annexin A4 [Zea mays] 0.95 860 1546 NP_001063096 Os09g0394900 [Oryza sativa 0.89 861 1547 Japonica Group] &gt; gi|49389155|dbj|BAD26449.1| putative annexin [Oryza sativa Japonica Group] &gt; gi|49389211|dbj|BAD26499.1| putative annexin [Oryza sativa Japonica Group] &gt; gi|113631329|dbj|BAF25010.1| Os09g0394900 [Oryza sativa Japonica Group] &gt; gi|215692709|dbj|BAG88129.1| unnamed protein product [Oryza sativa Japonica Group] &gt; gi|215704491|dbj|BAG93925.1| unnamed protein product [Oryza sativa Japonica Group] &gt; gi|222641513|gb|EEE69645.1| hypothetical protein OsJ_29255 [Oryza sativa Japonica Group] EEC84533 hypothetical protein 0.89 862 OsI_31267 [Oryza sativa Indica Group] NP_001132118 hypothetical protein 0.86 863 1548 LOC100193535 [Zea mays] &gt; gi|238908661|gb|ACF80822.2| unknown [Zea mays] BAJ85227 predicted protein [Hordeum 0.73 864 1549 vulgare subsp. vulgare] &gt; gi|326510845|dbj|BAJ91770.1| predicted protein [Hordeum vulgare subsp. vulgare] NP_001147343 annexin A4 [Zea mays] 0.72 865 1550 &gt; gi|195610314|gb|ACG26987.1| annexin A4 [Zea mays] &gt; gi|219887403|gb|ACL54076.1| unknown [Zea mays] AAR25142 annexin [Triticum aestivum] 0.73 866 1551 ACF06448 annexin [Elaeis guineensis] 0.74 867 1552 Predicted 891-914 ACG34432 L-allo-threonine aldolase [Zea 1.00 868 1553 folded mays] 24-nts- long seq 51802 ACG33933 L-allo-threonine aldolase [Zea 0.99 869 1554 mays] NP_001149239 L-allo-threonine aldolase [Zea 0.98 870 1555 mays] &gt; gi|195625716|gb|ACG34688.1| L-allo-threonine aldolase [Zea mays] NP_001053314 Os04g0516600 [Oryza sativa 0.79 871 1556 Japonica Group] &gt; gi|38344656|emb|CAD4182.21| OSJNBb0072M01.18 [Oryza sativa Japonica Group] &gt; gi|113564885|dbj|BAF15228.1| Os04g0516600 [Oryza sativa Japonica Group] &gt; gi|116310209|emb|CAH67219.1| OSIGBa0145M07.1 [Oryza sativa Indica Group] &gt; gi|215708770|dbj|BAG94039.1| unnamed protein product [Oryza sativa Japonica Group] &gt; gi|215765369|dbj|BAG87066.1| unnamed protein product [Oryza sativa Japonica Group] &gt; gi|218195217|gb|EEC77644.1| hypothetical protein OsI_16646 [Oryza sativa Indica Group] &gt; gi|222629209|gb|EEE61341.1| hypothetical protein OsJ_15466 [Oryza sativa Japonica Group] CAE05443 OSJNBa0073E02.3 [Oryza 0.75 872 1557 sativa Japonica Group] XP_002448187 hypothetical protein 0.79 873 1558 SORBIDRAFT_06g022630 [Sorghum bicolor] &gt; gi|241939370|gb|EES12515.1| hypothetical protein SORBIDRAFT_06g022630 [Sorghum bicolor] ACG36649 L-allo-threonine aldolase [Zea 0.80 874 1559 mays] BAK03835 predicted protein [Hordeum 0.79 875 1560 vulgare subsp. vulgare] BAJ93201 predicted protein [Hordeum 0.76 876 1561 vulgare subsp. vulgare] NP_001140767 hypothetical protein 0.72 877 1562 LOC100272842 [Zea mays] &gt; gi|194700996|gb|ACF84582.1| unknown [Zea mays] 700-723 AAS47590 phospholipid-hydroperoxide 1.00 878 1563 glutathione peroxidase [Setaria italica] XP_002446921 hypothetical protein 0.99 879 1564 SORBIDRAFT_06g024920 [Sorghum bicolor] &gt; gi|48374968|gb|AAT42166.1| putative glutathione peroxidase [Sorghum bicolor] &gt; gi|241938104|gb|EES11249.1| hypothetical protein SORBIDRAFT_06g024920 [Sorghum bicolor] NP_001141210 hypothetical protein 0.96 880 1565 LOC100273297 [Zea mays] &gt; gi|48374955|gb|AAT42154.1| putative glutathione peroxidase [Zea mays] &gt; gi|194703274|gb|ACF85721.1| unknown [Zea mays] &gt; gi|195622840|gb|ACG33250.1| phospholipid hydroperoxide glutathione peroxidase [Zea mays] &gt; gi|223975959|gb|ACN32167.1| unknown [Zea mays] ACG39625 phospholipid hydroperoxide 0.96 881 1566 glutathione peroxidase [Zea mays] EEC77777 hypothetical protein 0.95 882 OsI_16938 [Oryza sativa Indica Group] NP_001146472 hypothetical protein 0.94 883 1567 LOC100280060 [Zea mays] &gt; gi|219887431|gb|ACL54090.1| unknown [Zea mays] NP_001147681 phospholipid hydroperoxide 0.94 884 1568 glutathione peroxidase [Zea mays] &gt; gi|195613068|gb|ACG28364.1| phospholipid hydroperoxide glutathione peroxidase [Zea mays] NP_001053524 Os04g0556300 [Oryza sativa 0.94 885 1569 Japonica Group] &gt; gi|21360380|gb|AAM47493.1| glutathione peroxidase 1 [Oryza sativa] &gt; gi|113565095|dbj|BAF15438.1| Os04g0556300 [Oryza sativa Japonica Group] &gt; gi|215693018|dbj|BAG88438.1| unnamed protein product [Oryza sativa Japonica Group] &gt; gi|222629338|gb|EEE61470.1| hypothetical protein OsJ_15735 [Oryza sativa Japonica Group] NP_001105091 GP protein [Zea mays] 0.94 886 1570 &gt; gi|22268405|gb|AAM88847.2| AF520911_1 putative glutathione peroxidase [Zea mays] AAQ64633 cytosolic glutathione 0.93 887 1571 peroxidase [Triticum monococcum] 714-737 XP_002446921 hypothetical protein 1.00 888 1572 SORBIDRAFT_06g024920 [Sorghum bicolor] &gt; gi|48374968|gb|AAT42166.1| putative glutathione peroxidase [Sorghum bicolor] &gt; gi|241938104|gb|EES11249.1| hypothetical protein SORBIDRAFT_06g024920 [Sorghum bicolor] AAS47590 phospholipid-hydroperoxide 0.99 889 1573 glutathione peroxidase [Setaria italica] NP_001141210 hypothetical protein 0.98 890 1574 LOC100273297 [Zea mays] &gt; gi|48374955|gb|AAT42154.1| putative glutathione peroxidase [Zea mays] &gt; gi|194703274|gb|ACF85721.1| unknown [Zea mays] &gt; gi|195622840|gb|ACG33250.1| phospholipid hydroperoxide glutathione peroxidase [Zea mays] &gt; gi|223975959|gb|ACN32167.1| unknown [Zea mays] ACG39625 phospholipid hydroperoxide 0.97 891 1575 glutathione peroxidase [Zea mays] EEC77777 hypothetical protein 0.96 892 OsI_16938 [Oryza sativa Indica Group] NP_001146472 hypothetical protein 0.95 893 1576 LOC100280060 [Zea mays] &gt; gi|219887431|gb|ACL54090.1| unknown [Zea mays] NP_001147681 phospholipid hydroperoxide 0.95 894 1577 glutathione peroxidase [Zea mays] &gt; gi|195613068|gb|ACG28364.1| phospholipid hydroperoxide glutathione peroxidase [Zea mays] NP_001053524 Os04g0556300 [Oryza sativa 0.95 895 1578 Japonica Group] &gt; gi|21360380|gb|AAM47493.1| glutathione peroxidase 1 [Oryza sativa] &gt; gi|113565095|dbj|BAF15438.1| Os04g0556300 [Oryza sativa Japonica Group] &gt; gi|215693018|dbj|BAG88438.1| unnamed protein product [Oryza sativa Japonica Group] &gt; gi|222629338|gb|EEE61470.1| hypothetical protein OsJ_15735 [Oryza sativa Japonica Group] NP_001105091 GP protein [Zea mays] 0.95 896 1579 &gt; gi|22268405|gb|AAM88847.2| AF520911_1 putative glutathione peroxidase [Zea mays] AAQ64633 cytosolic glutathione 0.95 897 1580 peroxidase [Triticum monococcum] 160-183 NP_001159179 hypothetical protein 1.00 898 1581 LOC100304264 [Zea mays] &gt; gi|223942501|gb|ACN25334.1| unknown [Zea mays] NP_001105290 LOC542207 [Zea mays] 0.74 899 1582 &gt; gi|57791232|gb|AAW56446.1| methyl-binding domain protein MBD113 [Zea mays] 1054-1077 XP_002450144 hypothetical protein 1.00 900 1583 SORBIDRAFT_05g001160 [Sorghum bicolor] &gt; gi|241935987|gb|EES09132.1| hypothetical protein SORBIDRAFT_05g001160 [Sorghum bicolor] NP_001065992 Os12g0116000 [Oryza sativa 0.89 901 1584 Japonica Group] &gt; gi|108862099|gb|ABA96293.2| Vacuolar sorting receptor 1 precursor, putative, expressed [Oryza sativa Japonica Group] &gt; gi|113648499|dbj|BAF29011.1| Os12g0116000 [Oryza sativa Japonica Group] &gt; gi|215694863|dbj|BAG90054.1| unnamed protein product [Oryza sativa Japonica Group] EEC68730 hypothetical protein 0.89 902 OsI_37233 [Oryza sativa Indica Group] EEE51529 hypothetical protein 0.89 903 OsJ_32728 [Oryza sativa Japonica Group] EEC67546 hypothetical protein 0.89 904 OsI_34875 [Oryza sativa Indica Group] BAK03786 predicted protein [Hordeum 0.87 905 1585 vulgare subsp. vulgare] BAK00302 predicted protein [Hordeum 0.87 906 1586 vulgare subsp. vulgare] CBI18031 unnamed protein product 0.78 907 [Vitis vinifera] XP_002267833 PREDICTED: hypothetical 0.78 908 1587 protein [Vitis vinifera] XP_002309184 predicted protein [Populus 0.78 909 1588 trichocarpa] &gt; gi|222855160|gb|EEE92707.1| predicted protein [Populus trichocarpa] 1792-1815 NP_001140790 hypothetical protein 1.00 910 1589 LOC100272865 [Zea mays] &gt; gi|194701088|gb|ACF84628.1| unknown [Zea mays] XP_002464219 hypothetical protein 0.85 911 1590 SORBIDRAFT_01g014350 [Sorghum bicolor] &gt; gi|241918073|gb|EER91217.1| hypothetical protein SORBIDRAFT_01g014350 [Sorghum bicolor] XP_002464218 hypothetical protein 0.84 912 1591 SORBIDRAFT_01g014340 [Sorghum bicolor] &gt; gi|241918072|gb|EER91216.1| hypothetical protein SORBIDRAFT_01g014340 [Sorghum bicolor] AAL73972 putative cytochrome P450-like 0.73 913 1592 protein [Sorghum bicolor] 2477-2500 XP_002454227 hypothetical protein 1.00 914 1593 SORBIDRAFT_04g027100 [Sorghum bicolor] &gt; gi|241934058|gb|EES07203.1| hypothetical protein SORBIDRAFT_04g027100 [Sorghum bicolor] NP_001169751 hypothetical protein 0.93 915 1594 LOC100383632 [Zea mays] &gt; gi|224031415|gb|ACN34783.1| unknown [Zea mays] BAJ95386 predicted protein [Hordeum 0.78 916 1595 vulgare subsp. vulgare] EEC73665 hypothetical protein 0.77 917 OsI_08209 [Oryza sativa Indica Group] NP_001047529 Os02g0637800 [Oryza sativa 0.77 918 1596 Japonica Group] &gt; gi|49387984|dbj|BAD25092.1| unknown protein [Oryza sativa Japonica Group] &gt; gi|49388709|dbj|BAD25890.1| unknown protein [Oryza sativa Japonica Group] &gt; gi|113537060|dbj|BAF09443.1| Os02g0637800 [Oryza sativa Japonica Group] &gt; gi|215704166|dbj|BAG93006.1| unnamed protein product [Oryza sativa Japonica Group] &gt; gi|222623311|gb|EEE57443.1| hypothetical protein OsJ_07658 [Oryza sativa Japonica Group] 3108-3131 NP_001169691 hypothetical protein 1.00 919 1597 LOC100383572 [Zea mays] &gt; gi|224030935|gb|ACN34543.1| unknown [Zea mays] ACN33688 unknown [Zea mays] 0.96 920 1598 NP_001131248 hypothetical protein 0.81 921 1599 LOC100192560 [Zea mays] &gt; gi|195656461|gb|ACG47698.1| nucleic acid binding protein [Zea mays] XP_002441761 hypothetical protein 0.80 922 1600 SORBIDRAFT_08g001980 [Sorghum bicolor] &gt; gi|241942454|gb|EES15599.1| hypothetical protein SORBIDRAFT_08g001980 [Sorghum bicolor] 2192-2215 NP_001150353 LOC100283983 [Zea mays] 1.00 923 1601 &gt; gi|195638608|gb|ACG38772.1| ubiquilin-1 [Zea mays] &gt; gi|195644430|gb|ACG41683.1| ubiquilin-1 [Zea mays] &gt; gi|224029247|gb|ACN33699.1| unknown [Zea mays] XP_002465921 hypothetical protein 0.92 924 1602 SORBIDRAFT_01g048260 [Sorghum bicolor] &gt; gi|241919775|gb|EER92919.1| hypothetical protein SORBIDRAFT_01g048260 [Sorghum bicolor] NP_001048862 Os03g0131300 [Oryza sativa 0.78 925 1603 Japonica Group] &gt; gi|108706017|gb|ABF93812.1| Ubiquitin family protein, expressed [Oryza sativa Japonica Group] &gt; gi|113547333|dbj|BAF10776.1| Os03g0131300 [Oryza sativa Japonica Group] &gt; gi|215713564|dbj|BAG94701.1| unnamed protein product [Oryza sativa Japonica Group] &gt; gi|222624144|gb|EEE58276.1| hypothetical protein OsJ_09294 [Oryza sativa Japonica Group] EEC74450 hypothetical protein 0.78 926 OsI_09858 [Oryza sativa Indica Group] NP_001169509 hypothetical protein 0.78 927 1604 LOC100383383 [Zea mays] &gt; gi|224029753|gb|ACN33952.1| unknown [Zea mays] EAY79386 hypothetical protein 0.71 928 OsI_34513 [Oryza sativa Indica Group] N_001065193 Os10g0542200 [Oryza sativa 0.71 929 1605 Japonica Group] &gt; gi|10140758|gb|AAG13589.1| AC051633_5 putative ubiquitin protein [Oryza sativa Japonica Group] &gt; gi|31433330|gb|AAP54859.1| Ubiquitin family protein, expressed [Oryza sativa Japonica Group] &gt; gi|113639802|dbj|BAF27107.1| Os10g0542200 [Oryza sativa Japonica Group] EEE51337 hypothetical protein 0.71 930 OsJ_32327 [Oryza sativa Japonica Group] BAJ87273 predicted protein [Hordeum 0.73 931 1606 vulgare subsp. vulgare] BAK06552 predicted protein [Hordeum 0.74 932 1607 vulgare subsp. vulgare] 1289-1312 NP_001046031 Os02g0171100 [Oryza sativa 1.00 933 1608 Japonica Group] &gt; gi|49387565|dbj|BAD25496.1| putative glyceraldehyde- 3-phosphate dehydrogenase [Oryza sativa Japonica Group] &gt; gi|49388082|dbj|BAD25194.1| putative glyceraldehyde- 3-phosphate dehydrogenase [Oryza sativa Japonica Group] &gt; gi|113535562|dbj|BAF07945.1| Os02g0171100 [Oryza sativa Japonica Group] &gt; gi|215697739|dbj|BAG91733.1| unnamed protein product [Oryza sativa Japonica Group] &gt; gi|218190154|gb|EEC72581.1| hypothetical protein OsI_06028 [Oryza sativa Indica Group] &gt; gi|222622270|gb|EEE56402.1| hypothetical protein OsJ_05558 [Oryza sativa Japonica Group] NP_001149357 LOC100282981 [Zea mays] 0.92 934 1609 &gt; gi|195626622|gb|ACG35141.1| glyceraldehyde-3- phosphate dehydrogenase, cytosolic [Zea mays] &gt; gi|195641440|gb|ACG40188.1| glyceraldehyde-3- phosphate dehydrogenase, cytosolic [Zea mays] ACL54292 unknown [Zea mays] 0.92 935 1610 XP_002451619 hypothetical protein 0.90 936 1611 SORBIDRAFT_04g004750 [Sorghum bicolor] &gt; gi|241931450|gb|EES04595.1| hypothetical protein SORBIDRAFT_04g004750 [Sorghum bicolor] NP_001130796 hypothetical protein 0.88 937 1612 LOC100191900 [Zea mays] &gt; gi|194690136|gb|ACF79152.1| unknown [Zea mays] &gt; gi|195622606|gb|ACG33133.1| glyceraldehyde-3- phosphate dehydrogenase, cytosolic [Zea mays] &gt; gi|195625996|gb|ACG34828.1| glyceraldehyde-3- phosphate dehydrogenase, cytosolic [Zea mays] NP_001058309 Os06g0666600 [Oryza sativa 0.85 938 1613 Japonica Group] &gt; gi|52076528|dbj|BAD45405.1| putative glyceraldehyde- 3-phosphate dehydrogenase [Oryza sativa Japonica Group] &gt; gi|113596349|dbj|BAF20223.1| Os06g0666600 [Oryza sativa Japonica Group] &gt; gi|125556399|gb|EAZ02005.1| hypothetical protein OsI_24036 [Oryza sativa Indica Group] &gt; gi|215741351|dbj|BAG97846.1| unnamed protein product [Oryza sativa Japonica Group] BAJ86344 predicted protein [Hordeum 0.82 939 1614 vulgare subsp. vulgare] &gt; gi|326520716|dbj|BAJ92721.1| predicted protein [Hordeum vulgare subsp. vulgare] ADJ96634 glyceraldehyde-3-phosphate 0.82 940 1615 dehydrogenase [Guzmania wittmackii x Guzmania lingulata] CAC80377 glyceraldehyde-3-phosphate 0.77 941 1616 dehydrogenase [Capsicum annuum] &gt; gi|26986729|emb|CAC88118.1| glyceraldehyde-3- phosphate dehydrogenase [Capsicum annuum] XP_002263263 PREDICTED: hypothetical 0.75 942 1617 protein [Vitis vinifera] &gt; gi|297736130|emb|CBI24168.3| unnamed protein product [Vitis vinifera] Predicted 173-196 AAL76004 putative gag-pol polyprotein 1.00 943 folded [Zea mays] 24-nts- long seq 52801 AAN40035 putative gag-pol polyprotein 0.90 944 [Zea mays] AAN40025 putative gag-pol polyprotein 0.85 945 [Zea mays] AAL66753 putative copia-type pol 0.81 946 polyprotein [Zea mays] AAP94599 putative copia-type pol 0.81 947 polyprotein [Zea mays] Predicted 14-37 XP_002458660 hypothetical protein 1.00 948 1618 folded SORBIDRAFT_03g037610 24-nts- [Sorghum bicolor] long seq &gt; gi|241930635|gb|EES03780.1| 52452 hypothetical protein SORBIDRAFT_03g037610 [Sorghum bicolor] NP_001106055 ribonuclease III domain 0.98 949 1619 protein1 [Zea mays] &gt; gi|149980667|gb|ABR53724.1| chloroplast ribonuclease III domain protein [Zea mays] &gt; gi|194704042|gb|ACF86105.1| unknown [Zea mays] &gt; gi|195647406|gb|ACG43171.1| RNA binding protein [Zea mays] &gt; gi|223950477|gb|ACN29322.1| unknown [Zea mays] BAJ85766 predicted protein [Hordeum 0.91 950 1620 vulgare subsp. vulgare] &gt; gi|326503490|dbj|BAJ86251.1| predicted protein [Hordeum vulgare subsp. vulgare] &gt; gi|326520661|dbj|BAJ92694.1| predicted protein [Hordeum vulgare subsp. vulgare] EEE55556 hypothetical protein 0.89 951 OsJ_03820 [Oryza sativa Japonica Group] NP_001044581 Os01g0810100 [Oryza sativa 0.92 952 1621 Japonica Group] &gt; gi|20160696|dbj|BAB89639.1| unknown protein [Oryza sativa Japonica Group] &gt; gi|113534112|dbj|BAF06495.1| Os01g0810100 [Oryza sativa Japonica Group] &gt; gi|215701330|dbj|BAG92754.1| unnamed protein product [Oryza sativa Japonica Group] &gt; gi|215704464|dbj|BAG93898.1| unnamed protein product [Oryza sativa Japonica Group] EEC71679 hypothetical protein 0.86 953 OsI_04153 [Oryza sativa Indica Group] BAJ91324 predicted protein [Hordeum 0.85 954 1622 vulgare subsp. vulgare] XP_002533159 RNA binding protein, putative 0.75 955 1623 [Ricinus communis] &gt; gi|223527031|gb|EEF29218.1| RNA binding protein, putative [Ricinus communis] NP_195467 Ribonuclease III family 0.74 956 1624 protein [Arabidopsis thaliana] &gt; gi|4468817|emb|CAB38218.1| putative protein [Arabidopsis thaliana] &gt; gi|7270733|emb|CAB80416.1| putative protein [Arabidopsis thaliana] &gt; gi|95147308|gb|ABF57289.1| At4g37510 [Arabidopsis thaliana] &gt; gi|332661403|gb|AEE86803.1| Ribonuclease III family protein [Arabidopsis thaliana] XP_002866942 ribonuclease III family protein 0.74 957 1625 [Arabidopsis lyrata subsp. lyrata] &gt; gi|297312778|gb|EFH43201.1| ribonuclease III family protein [Arabidopsis lyrata subsp. lyrata]  86-109 NP_001169273 hypothetical protein 1.00 958 1626 LOC100383136 [Zea mays] &gt; gi|224028313|gb|ACN33232.1| unknown [Zea mays] XP_022462951 hypothetical protein 0.94 959 1627 SORBIDRAFT_02g035180 [Sorghum bicolor] &gt; gi|241926328|gb|EER99472.1| hypothetical protein SORBIDRAFT_02g035180 [Sorghum bicolor] BAK06716 predicted protein [Hordeum 0.82 960 1628 vulgare subsp. vulgare] EEC82174 hypothetical protein 0.81 961 OsI_26284 [Oryza sativa Indica Group] NP_001059844 Os07g0530100 [Oryza sativa 0.81 962 1629 Japonica Group] &gt; gi|33146648|dbj|BAC79984.1| putative tyrosyl-DNA phosphodiesterase [Oryza sativa Japonica Group] &gt; gi|113611380|dbj|BAF21758.1| Os07g0530100 [Oryza sativa Japonica Group] &gt; gi|215697362|dbj|BAG91356.1| unnamed protein product [Oryza sativa Japonica Group] &gt; gi|222637174|gb|EEE67306.1| hypothetical protein OsJ_24533 [Oryza sativa Japonica Group] Predicted 69-89 NP_001167972 hypothetical protein 1.00 zma LOC100381689 [Zea mays] mir &gt; gi|223945237|gb|ACN26702.1| 48588 unknown [Zea mays] zma- 1395-1415 NP_001151161 riboflavin kinase family 1.00 963 1630 miR166d* protein [Zea mays] &gt; gi|195644710|gb|ACG41823.1| riboflavin kinase family protein [Zea mays] EEC76352 hypothetical protein 0.78 964 OsI_13934 [Oryza sativa Indica Group] ABF99395 riboflavin biosynthesis protein, 0.77 965 1631 putative, expressed [Oryza sativa Japonica Group] NP_001051594 Os03g0801700 [Oryza sativa 0.77 966 1632 Japonica Group] &gt; gi|108711599|gb|ABF99394.1| riboflavin biosynthesis protein, putative, expressed [Oryza sativa Japonica Group] &gt; gi|113550065|dbj|BAF13508.1| Os03g0801700 [Oryza sativa Japonica Group] &gt; gi|222625981|gb|EEE60113.1| hypothetical protein OsJ_12987 [Oryza sativa Japonica Group] BAK03408 predicted protein [Hordeum 0.73 967 1633 vulgare subsp. vulgare] BAJ90790 predicted protein [Hordeum 0.72 968 1634 vulgare subsp. vulgare] Predicted 923-946 XP_002461133 hypothetical protein 1.00 969 1635 folded SORBIDRAFT_02g041270 24-nts- [Sorghum bicolor] long seq &gt; gi|241924510|gb|EER97654.1| 52795 hypothetical protein SORBIDRAFT_02g041270 [Sorghum bicolor] ACR36108 unknown [Zea mays] 0.97 970 1636 NP_001149600 protein SFT2 [Zea mays] 0.96 971 1637 &gt; gi|195628366|gb|ACG36013.1| protein SFT2 [Zea mays] BAJ93024 predicted protein [Hordeum 0.90 972 1638 vulgare subsp. vulgare] BAJ93185 predicted protein [Hordeum 0.90 973 1639 vulgare subsp. vulgare] NP_001057449 Os06g0300300 [Oryza sativa 0.89 974 1640 Japonica Group] &gt; gi|53792521|dbj|BAD53485.1| unknown protein [Oryza sativa Japonica Group] &gt; gi|113595489|dbj|BAF19363.1| Os06g0300300 [Oryza sativa Japonica Group] &gt; gi|215697111|dbj|BAG91105.1| unnamed protein product [Oryza sativa Japonica Group] &gt; gi|215766579|dbj|BAG98738.1| unnamed protein product [Oryza sativa Japonica Group] &gt; gi|218198021|gb|EEC80448.1| hypothetical protein OsI_22649 [Oryza sativa Indica Group] &gt; gi|222635438|gb|EEE65570.1| hypothetical protein OsJ_21068 [Oryza sativa Japonica Group] XP_002532175 Protein transport protein SFT2, 0.73 975 1641 putative [Ricinus communis] &gt; gi|223528143|gb|EEF30212.1| Protein transport protein SFT2, putative [Ricinus communis] XP_002264182 PREDICTED: hypothetical 0.70 976 1642 protein [Vitis vinifera] &gt; gi|296085064|emb|CBI28479.3| unnamed protein product [Vitis vinifera] 149-172 XP_002445951 hypothetical protein 1.00 977 1643 SORBIDRAFT_07g028610 [Sorghum bicolor] &gt; gi|241942301|gb|EES15446.1| hypothetical protein SORBIDRAFT_07g028610 [Sorghum bicolor] NP_001131191 hypothetical protein 0.92 978 1644 LOC100192499 [Zea mays] &gt; gi|194690830|gb|ACF79499.1| unknown [Zea mays] BAK06465 predicted protein [Hordeum 0.83 979 1645 vulgare subsp. vulgare] &gt; gi|326515200|dbj|BAK03513.1| predicted protein [Hordeum vulgare subsp. vulgare] EAZ07476 hypothetical protein 0.83 980 OsI_29735 [Oryza sativa Indica Group] &gt; gi|125603873|gb|EAZ43198.1| hypothetical protein OsJ_27795 [Oryza sativa Japonica Group] NP_001062134 Os08g0496000 [Oryza sativa 0.83 981 1646 Japonica Group] &gt; gi|42408865|dbj|BAD10124.1| putative cytochrome c oxidase subunit 15(COX15) homolog isoform 1 precursor [Oryza sativa Japonica Group] &gt; gi|113624103|dbj|BAF24048.1| Os08g0496000 [Oryza sativa Japonica Group] &gt; gi|215737013|dbj|BAG95942.1| unnamed protein product [Oryza sativa Japonica Group] zma- 107-128 XP_002451684 hypothetical protein 1.00 992 1655 miR169c* SORBIDRAFT_04g005850 [Sorghum bicolor] &gt; gi|241931515|gb|EES04660.1| hypothetical protein SORBIDRAFT_04g005850 [Sorghum bicolor] NP_001169303 hypothetical protein 0.85 993 1656 LOC100383167 [Zea mays] &gt; gi|195627360|gb|ACG35510.1| cytochrome P450 CYP71K14 [Zea mays] ACN33338 unknown [Zea mays] 0.76 994 1657 1135-1156 NP_001169303 hypothetical protein 1.00 995 1658 LOC100383167 [Zea mays] &gt; gi|195627360|gb|ACG35510.1| cytochrome P450 CYP71K14 [Zea mays] ACN33338 unknown [Zea mays] 0.88 996 1659 XP_002451684 hypothetical protein 0.85 997 1660 SORBIDRAFT_04g005850 [Sorghum bicolor] &gt; gi|241931515|gb|EES04660.1| hypothetical protein SORBIDRAFT_04g005850 [Sorghum bicolor] Predicted 606-625 XP_002436376 hypothetical protein 1.00 998 1661 zma SORBIDRAFT_10g001390 mir [Sorghum bicolor] 50517 &gt; gi|241914599|gb|EER87743.1| hypothetical protein SORBIDRAFT_10g001390 [Sorghum bicolor] NP_001150208 DAG protein [Zea mays] 0.81 999 1662 &gt; gi|195637572|gb|ACG38254.1| DAG protein [Zea mays] &gt; gi|223947219|gb|ACN27693.1| unknown [Zea mays] BAC22214 putative plastid protein [Oryza 0.81 1000 1663 sativa Japonica Group] BAK01694 predicted protein [Hordeum 0.80 1001 1664 vulgare subsp. vulgare] BAJ87670 predicted protein [Hordeum 0.76 1002 1665 vulgare subsp. vulgare] 560-579 NP_001056622 Os06g0116600 [Oryza sativa 1.00 1003 1666 Japonica Group] &gt; gi|55296199|dbj|BAD67917.1| putative DAL1 protein [Oryza sativa Japonica Group] &gt; gi|113594662|dbj|BAF18536.1| Os06g0116600 [Oryza sativa Japonica Group] &gt; gi|218197457|gb|EEC79884.1| hypothetical protein OsI_21391 [Oryza sativa Indica Group] &gt; gi|222634859|gb|EEE64991.1| hypothetical protein OsJ_19911 [Oryza sativa Japonica Group] 3352-3371 NP_001170545 hypothetical protein 1.00 1004 1667 LOC100384563 [Zea mays] &gt; gi|238005944|gb|ACR34007.1| unknown [Zea mays] XP_002436375 hypothetical protein 0.85 1005 1668 SORBIDRAFT_10g001380 [Sorghum bicolor] &gt; gi|241914598|gb|EER87742.1| hypothetical protein SORBIDRAFT_10g001380 [Sorghum bicolor] EEC79883 hypothetical protein 0.81 1006 OsI_21390 [Oryza sativa Indica Group] EEE64990 hypothetical protein 0.78 1007 OsJ_19910 [Oryza sativa Japonica Group] NP_001045600 Os02g0102800 [Oryza sativa 0.75 1008 1669 Japonica Group] &gt; gi|41052906|dbj|BAD07818.1| unknown protein [Oryza sativa Japonica Group] &gt; gi|41053240|dbj|BAD08201.1| unknown protein [Oryza sativa Japonica Group] &gt; gi|113535131|dbj|BAF07514.1| Os02g0102800 [Oryza sativa Japonica Group] Predicted 965-985 NP_001141527 hypothetical protein 1.00 1009 1670 zma LOC100273639 [Zea mays] mir &gt; gi|194704936|gb|ACF86552.1| 49435 unknown [Zea mays] &gt; gi|195642904|gb|ACG40920.1| hypothetical protein [Zea mays] XP_002440488 hypothetical protein 0.85 1010 1671 SORBIDRAFT_09g001840 [Sorghum bicolor] &gt; gi|241945773|gb|EES18918.1| hypothetical protein SORBIDRAFT_09g001840 [Sorghum bicolor] 358-378 ACF85023 unknown [Zea mays] 1.00 1011 1672 NP_001151177 transposon protein [Zea mays] 0.99 1012 1673 &gt; gi|195644822|gb|ACG41879.1| transposon protein [Zea mays] XP_002452722 hypothetical protein 0.86 1013 1674 SORBIDRAFT_04g031370 [Sorghum bicolor] &gt; gi|241932553|gb|EES05698.1| hypothetical protein SORBIDRAFT_04g031370 [Sorghum bicolor] NP_001047784 Os02g0689500 [Oryza sativa 0.77 1014 1675 Japonica Group] &gt; gi|41052806|dbj|BAD07674.1| unknown protein [Oryza sativa Japonica Group] &gt; gi|113537315|dbj|BAF09698.1| Os02g0689500 [Oryza sativa Japonica Group] &gt; gi|125540730|gb|EAY87125.1| hypothetical protein OsI_08527 [Oryza sativa Indica Group] &gt; gi|215704259|dbj|BAG93099.1| unnamed protein product [Oryza sativa Japonica Group] mtr- 174-194 XP_002468544 hypothetical protein 1.00 1015 1676 miR169q SORBIDRAFT_01g047710 [Sorghum bicolor] &gt; gi|241922398|gb|EER95542.1| hypothetical protein SORBIDRAFT_01g047710 [Sorghum bicolor] NP_001142595 hypothetical protein 0.85 1016 1677 LOC100274862 [Zea mays] &gt; gi|195607096|gb|ACG25378.1| hypothetical protein [Zea mays] ACN31627 unknown [Zea mays] 0.85 1017 1678 ACR38267 unknown [Zea mays] 0.85 1018 1679 NP_001144209 hypothetical protein 0.85 1019 1680 LOC100277070 [Zea mays] &gt; gi|195638456|gb|ACG38696.1| hypothetical protein [Zea mays] NP_001048909 Os03g0138500 [Oryza sativa 0.72 1020 1681 Japonica Group] &gt; gi|108706085|gb|ABF93880.1| expressed protein [Oryza sativa Japonica Group] &gt; gi|113547380|dbj|BAF10823.1| Os03g0138500 [Oryza sativa Japonica Group] EAZ25513 hypothetical protein 0.71 1021 OsJ_09336 [Oryza sativa Japonica Group] EAY88465 hypothetical protein 0.71 1022 OsI_09934 [Oryza sativa Indica Group] 1074-1094 NP_001150090 PIT1 [Zea mays] 1.00 1023 1682 &gt; gi|195636634|gb|ACG37785.1| PIT1 [Zea mays] NP_001042834 Os01g0303600 [Oryza sativa 0.76 1024 1683 Japonica Group] &gt; gi|52075677|dbj|BAD44897.1| zinc finger protein-like [Oryza sativa Japonica Group] &gt; gi|52077476|dbj|BAD45040.1| zinc finger protein-like [Oryza sativa Japonica Group] &gt; gi|218188061|gb|EEC70488.1| hypothetical protein OsI_01559 [Oryza sativa Indica Group] &gt; gi|255673151|dbj|BAF04748.2| Os01g0303600 [Oryza sativa Japonica Group] Predicted 71-92 XP_002437560 hypothetical protein 1.00 1025 1684 zma SORBIDRAFT_10g029400 mir [Sorghum bicolor] 49003 &gt; gi|241915783|gb|EER88927.1| hypothetical protein SORBIDRAFT_10g029400 [Sorghum bicolor] ACL53917 unknown [Zea mays] 0.97 1026 1685 NP_001152313 LOC100285952 [Zea mays] 0.97 1027 1686 &gt; gi|195654989|gb|ACG46962.1| MPK17-1-putative MAPK [Zea mays] NP_001146196 LOC100279766 [Zea mays] 0.95 1028 1687 &gt; gi|219886151|gb|ACL53450.1| unknown [Zea mays] &gt; gi|297595201|gb|ADI48121.1| putative mitogen-activated protein kinase 17-3 [Zea mays] EEC81289 hypothetical protein 0.91 1029 OsI_24409 [Oryza sativa Indica Group] NP_001058530 Os06g0708000 [Oryza sativa 0.91 1030 1688 Japonica Group] &gt; gi|108860803|sp|Q5Z9J0.2| MPK12_ORYSJ RecName: Full = Mitogen-activated protein kinase 12; Short = MAP kinase 12; AltName: Full = Blast- and wound- induced MAP kinase 1; AltName: Full = MAP kinase 1; AltName: Full = OsBWMK1; AltName: Full = OsMAPK1 &gt; gi|53792601|dbj|BAD53616.1| putative MAP kinase [Oryza sativa Japonica Group] &gt; gi|113596570|dbj|BAF20444.1| Os06g0708000 [Oryza sativa Japonica Group] AAX20165 putative MAPK protein kinase 0.86 1031 1689 [Triticum aestivum] &gt; gi|84795226|gb|ABC65849.1| mitogen-activated protein kinase MAPK1e [Triticum aestivum] ABC54587 mitogen-activated protein 0.86 1032 1690 kinase [Triticum aestivum] AAX20166 putative MAPK protein kinase 0.86 1033 1691 [Triticum aestivum] ABD97883 mitogen-activated protein 0.86 1034 1692 kinase [Triticum aestivum] 53-74 EAY76957 hypothetical protein 1.00 1035 OsI_04915 [Oryza sativa Indica Group] NP_001045192 Os01g0916200 [Oryza sativa 1.00 1036 1693 Japonica Group] &gt; gi|19386749|dbj|BAB86130.1| putative adapter-related protein complex 4 epsilon 1 subunit [Oryza sativa Japonica Group] &gt; gi|20805003|dbj|BAB92679.1| putative adapter-related protein complex 4 epsilon 1 subunit [Oryza sativa Japonica Group] &gt; gi|113534723|dbj|BAF07106.1| Os01g0916200 [Oryza sativa Japonica Group] &gt; gi|215707205|dbj|BAG93665.1| unnamed protein product [Oryza sativa Japonica Group] EAZ14613 hypothetical protein 0.93 1037 OsJ_04538 [Oryza sativa Japonica Group] XP_002458982 hypothetical protein 0.91 1038 1694 SORBIDRAFT_03g043730 [Sorghum bicolor] &gt; gi|241930957|gb|EES04102.1| hypothetical protein SORBIDRAFT_03g043730 [Sorghum bicolor] NP_001169710 hypothetical protein 0.90 1039 1695 LOC100383591 [Zea mays] &gt; gi|224031083|gb|ACN34617.1| unknown [Zea mays] BAJ89128 predicted protein [Hordeum 0.88 1040 1696 vulgare subsp. vulgare] XP_002328755 predicted protein [Populus 0.70 1041 1697 trichocarpa] &gt; gi|222839053|gb|EEE77404.1| predicted protein [Populus trichocarpa] XP_002519936 AP-1 complex subunit 0.71 1042 1698 gamma-2, putative [Ricinus communis] &gt; gi|223540982|gb|EEF42540.1| AP-1 complex subunit gamma-2, putative [Ricinus communis] zma- 897-917 NP_001105061 Hageman factor inhibitor [Zea 1.00 1043 1699 miR399g mays] &gt; gi|16305146|gb|AAL16995.1| Hageman factor inhibitor [Zea mays] NP_001106233 trypsin/factor XIIA inhibitor 0.84 1044 1700 precursor [Zea mays] &gt; gi|266398|sp|P01088.2|ITRF_MAIZE RecName: Full = Trypsin/factor XIIA inhibitor; AltName: Full = CHFI; AltName: Full = Hageman factor inhibitor; Flags: Precursor &gt; gi|22327|emb|CAA37998.1| corn Hageman factor inhibitor [Zea mays] &gt; gi|75994173|gb|ABA34122.1| hageman factor inhibitor [Zea mays subsp. parviglumis] &gt; gi|75994177|gb|ABA34124.1| hageman factor inhibitor [Zea mays subsp. parviglumis] &gt; gi|75994189|gb|ABA34130.1| hageman factor inhibitor [Zea mays subsp. parviglumis] &gt; gi|75994193|gb|ABA34132.1| hageman factor inhibitor [Zea mays subsp. parviglumis] &gt; gi|195658559|gb|ACG48747.1| trypsin/factor XIIA inhibitor precursor [Zea mays] &gt; gi|195658619|gb|ACG48777.1| trypsin/factor XIIA inhibitor precursor [Zea mays] &gt; gi|214014768|gb|ACJ62034.1| Hageman factor inhibitor [Zea mays subsp. parviglumis] &gt; gi|214014812|gb|ACJ62056.1| Hageman factor inhibitor [Zea mays subsp. parviglumis] &gt; gi|214014814|gb|ACJ62057.1| Hageman factor inhibitor [Zea mays subsp. parviglumis] &gt; gi|214014820|gb|ACJ62060.1| Hageman factor inhibitor [Zea mays subsp. parviglumis] &gt; gi|214014826|gb|ACJ62063.1| Hageman factor inhibitor [Zea mays subsp. parviglumis] &gt; gi|214014828|gb|ACJ62064.1| Hageman factor inhibitor [Zea mays subsp. parviglumis] &gt; gi|214014862|gb|ACJ62081.1| Hageman factor inhibitor [Zea mays subsp. parviglumis] &gt; gi|214014864|gb|ACJ62082.1| Hageman factor inhibitor [Zea mays subsp. parviglumis] &gt; gi|214014870|gb|ACJ62085.1| Hageman factor inhibitor [Zea mays subsp. parviglumis] &gt; gi|238014562|gb|ACR38316.1| unknown [Zea mays] ABA34135 hageman factor inhibitor [Zea 0.83 1045 1701 mays subsp. parviglumis] &gt; gi|214014790|gb|ACJ62045.1| Hageman factor inhibitor [Zea mays subsp. parviglumis] &gt; gi|214014796|gb|ACJ62048.1| Hageman factor inhibitor [Zea mays subsp. parviglumis] ACJ62011 Hageman factor inhibitor [Zea 0.83 1046 1702 mays subsp. parviglumis] &gt; gi|214014726|gb|ACJ62013.1| Hageman factor inhibitor [Zea mays subsp. parviglumis] &gt; gi|214014728|gb|ACJ62014.1| Hageman factor inhibitor [Zea mays subsp. parviglumis] &gt; gi|214014730|gb|ACJ62015.1| Hageman factor inhibitor [Zea mays subsp. parviglumis] &gt; gi|214014732|gb|ACJ62016.1| Hageman factor inhibitor [Zea mays subsp. parviglumis] &gt; gi|214014734|gb|ACJ62017.1| Hageman factor inhibitor [Zea mays subsp. parviglumis] &gt; gi|214014744|gb|ACJ62022.1| Hageman factor inhibitor [Zea mays subsp. parviglumis] &gt; gi|214014746|gb|ACJ62023.1| Hageman factor inhibitor [Zea mays subsp. parviglumis] &gt; gi|214014748|gb|ACJ62024.1| Hageman factor inhibitor [Zea mays subsp. parviglumis] ABA34133 hageman factor inhibitor [Zea 0.83 1047 1703 mays subsp. parviglumis] ACJ62080 Hageman factor inhibitor [Zea 0.83 1048 1704 mays subsp. parviglumis] ABA34137 hageman factor inhibitor [Zea 0.83 1049 1705 diploperennis] &gt; gi|75994207|gb|ABA34139.1| hageman factor inhibitor [Zea diploperennis] &gt; gi|75994211|gb|ABA34141.1| hageman factor inhibitor [Zea diploperennis] &gt; gi|75994213|gb|ABA34142.1| hageman factor inhibitor [Zea diploperennis] &gt; gi|75994215|gb|ABA34143.1| hageman factor inhibitor [Zea diploperennis] &gt; gi|75994217|gb|ABA34144.1| hageman factor inhibitor [Zea diploperennis] ACG48546 trypsin/factor XIIA inhibitor 0.83 1050 1706 precursor [Zea mays] ABA34129 hageman factor inhibitor [Zea 0.83 1051 1707 mays subsp. parviglumis] ABA34125 hageman factor inhibitor [Zea 0.83 1052 1708 mays subsp. parviglumis] &gt; gi|75994185|gb|ABA34128.1| hageman factor inhibitor [Zea mays subsp. parviglumis] &gt; gi|214014736|gb|ACJ62018.1| Hageman factor inhibitor [Zea mays subsp. parviglumis] &gt; gi|214014752|gb|ACJ62026.1| Hageman factor inhibitor [Zea mays subsp. parviglumis] &gt; gi|214014822|gb|ACJ62061.1| Hageman factor inhibitor [Zea mays subsp. parviglumis] Predicted 379-399 XP_002454327 hypothetical protein 1.00 1053 1709 zma SORBIDRAFT_04g028730 mir [Sorghum bicolor] 49985 &gt; gi|241934158|gb|EES07303.1| hypothetical protein SORBIDRAFT_04g028730 [Sorghum bicolor] ACG36841 caleosin related protein [Zea 0.81 1054 1710 mays] ACN31536 unknown [Zea mays] 0.78 1055 1711 NP_001142815 hypothetical protein 0.79 1056 1712 LOC100275194 [Zea mays] &gt; gi|195610158|gb|ACG26909.1| hypothetical protein [Zea mays] NP_001148700 caleosin related protein [Zea 0.77 1057 1713 mays] &gt; gi|195621488|gb|ACG32574.1| caleosin related protein [Zea mays] 632-652 XP_002448765 hypothetical protein 1.00 1058 1714 SORBIDRAFT_06g032800 [Sorghum bicolor] &gt; gi|241939948|gb|EES13093.1| hypothetical protein SORBIDRAFT_06g032800 [Sorghum bicolor] NP_001131374 hypothetical protein 0.99 1059 1715 LOC100192699 [Zea mays] &gt; gi|195620734|gb|ACG32197.1| oligosaccharyl transferase STT3 subunit [Zea mays] NP_001168720 hypothetical protein 0.98 1060 1716 LOC100382512 [Zea mays] &gt; gi|223950395|gb|ACN29281.1| unknown [Zea mays] NP_001054248 Os04g0675500 [Oryza sativa 0.96 1061 1717 Japonica Group] &gt; gi|38344929|emb|CAE03245.2| OSJNBa0018M05.20 [Oryza sativa Japonica Group] &gt; gi|90399055|emb|CAJ86104.1| H0103C06.8 [Oryza sativa Indica Group] &gt; gi|113565819|dbj|BAF16162.1| Os04g0675500 [Oryza sativa Japonica Group] &gt; gi|125550210|gb|EAY96032.1| hypothetical protein OsI_17905 [Oryza sativa Indica Group] &gt; gi|125592048|gb|EAZ32398.1| hypothetical protein OsJ_16609 [Oryza sativa Japonica Group] &gt; gi|215708677|dbj|BAG93946.1| unnamed protein product [Oryza sativa Japonica Group] BAJ87792 predicted protein [Hordeum 0.95 1062 1718 vulgare subsp. vulgare] CBG76274 OO_Ba0005L10- 0.93 1063 1719 OO_Ba0081K17.25 [Oryza officinalis] XP_002269119 PREDICTED: hypothetical 0.86 1064 1720 protein [Vitis vinifera] XP_002318345 predicted protein [Populus 0.86 1065 1721 trichocarpa] &gt; gi|222859018|gb|EEE96565.1| predicted protein [Populus trichocarpa] XP_002891078 hypothetical protein 0.85 1066 1722 ARALYDRAFT_891007 [Arabidopsis lyrata subsp. lyrata] &gt; gi|297336920|gb|EFH67337.1| hypothetical protein ARALYDRAFT_891007 [Arabidopsis lyrata subsp. lyrata] XP_002329687 predicted protein [Populus 0.85 1067 1723 trichocarpa] &gt; gi|222870595|gb|EEF07726.1| predicted protein [Populus trichocarpa] 795-815 XP_002458747 hypothetical protein 1.00 1068 1724 SORBIDRAFT_03g039530 [Sorghum bicolor] &gt; gi|241930722|gb|EES03867.1| hypothetical protein SORBIDRAFT_03g039530 [Sorghum bicolor] NP_001147942 L-ascorbate oxidase [Zea 0.96 1069 1725 mays] &gt; gi|195614732|gb|ACG29196.1| L-ascorbate oxidase precursor [Zea mays] ACN34362 unknown [Zea mays] 0.96 1070 1726 EEC71780 hypothetical protein 0.86 1071 OsI_04394 [Oryza sativa Indica Group] NP_001044773 Os01g0842500 [Oryza sativa 0.86 1072 1727 Japonica Group] &gt; gi|19571025|dbj|BAB86452.1| putative laccase LAC5-6 [Oryza sativa Japonica Group] &gt; gi|113534304|dbj|BAF06687.1| Os01g0842500 [Oryza sativa Japonica Group] &gt; gi|125572601|gb|EAZ14116.1| hypothetical protein OsJ_04040 [Oryza sativa Japonica Group] &gt; gi|215694814|dbj|BAG90005.1| unnamed protein product [Oryza sativa Japonica Group] BAJ84890 predicted protein [Hordeum 0.84 1073 1728 vulgare subsp. vulgare] BAJ96691 predicted protein [Hordeum 0.84 1074 1729 vulgare subsp. vulgare] NP_001105875 putative laccase [Zea mays] 0.79 1075 1730 &gt; gi|84618781|emb|CAJ30500.1| putative laccase [Zea mays] ACN28855 unknown [Zea mays] 0.79 1076 1731 XP_002458746 hypothetical protein 0.78 1077 1732 SORBIDRAFT_03g039520 [Sorghum bicolor] &gt; gi|241930721|gb|EES03866.1| hypothetical protein SORBIDRAFT_03g039520 [Sorghum bicolor] 198-218 XP_002458746 hypothetical protein 1.00 1078 1733 SORBIDRAFT_03g039520 [Sorghum bicolor] &gt; gi|241930721|gb|EES03866.1| hypothetical protein SORBIDRAFT_03g039520 [Sorghum bicolor] NP_001105875 putative laccase [Zea mays] 0.94 1079 1734 &gt; gi|84618781|emb|CAJ30500.1| putative laccase [Zea mays] NP_001146658 hypothetical protein 0.93 1080 1735 LOC100280258 [Zea mays] &gt; gi|219888209|gb|ACL54479.1| unknown [Zea mays] ACN28855 unknown [Zea mays] 0.94 1081 1736 NP_001044772 Os01g0842400 [Oryza sativa 0.80 1082 1737 Japonica Group] &gt; gi|75321217|sp|Q5N9X2.1| LAC4_ORYSJ RecName: Full = Laccase-4; AltName: Full = Benzenediol:oxygen oxidoreductase 4; AltName: Full = Diphenol oxidase 4; AltName: Full = Urishiol oxidase 4; Flags: Precursor &gt; gi|56784239|dbj|BAD81734.1| putative laccase LAC5-6 [Oryza sativa Japonica Group] &gt; gi|113534303|dbj|BAF06686.1| Os01g0842400 [Oryza sativa Japonica Group] &gt; gi|215697155|dbj|BAG91149.1| unnamed protein product [Oryza sativa Japonica Group] EAZ14115 hypothetical protein 0.80 1083 OsJ_04039 [Oryza sativa Japonica Group] EEC71777 hypothetical protein 0.80 1084 OsI_04389 [Oryza sativa Indica Group] BAJ99773 predicted protein [Hordeum 0.78 1085 1738 vulgare subsp. vulgare] XP_002458747 hypothetical protein 0.78 1086 1739 SORBIDRAFT_03g039530 [Sorghum bicolor] &gt; gi|241930722|gb|EES03867.1| hypothetical protein SORBIDRAFT_03g039530 [Sorghum bicolor] AAC04576 putative high-pI laccase 0.79 1087 1740 [Oryza sativa Japonica Group] 751-771 XP_002456566 hypothetical protein 1.00 1088 1741 SORBIDRAFT_03g038550 [Sorghum bicolor] &gt; gi|241928541|gb|EES01686.1| hypothetical protein SORBIDRAFT_03g038550 [Sorghum bicolor] NP_001148479 L-ascorbate oxidase [Zea 0.92 1089 1742 mays] &gt; gi|195619672|gb|ACG31666.1| L-ascorbate oxidase precursor [Zea mays] NP_001183899 hypothetical protein 0.90 1090 1743 LOC100502492 [Zea mays] &gt; gi|223948465|gb|ACN28316.1| unknown [Zea mays] &gt; gi|238015342|gb|ACR38706.1| unknown [Zea mays] NP_001044679 Os01g0827300 [Oryza sativa 0.84 1091 1744 Japonica Group] &gt; gi|75331868|sp|Q941X2.1| LAC3_ORYSJ RecName: Full = Benzenediol: oxygen oxidoreductase 3; AltName: Full = Diphenol oxidase 3; AltName: Full = Urishiol oxidase 3; Flags: Precursor &gt; gi|15624045|dbj|BAB68098.1| putative laccase [Oryza sativa Japonica Group] &gt; gi|113534210|dbj|BAF06593.1| Os01g0827300 [Oryza sativa Japonica Group] &gt; gi|215701334|dbj|BAG92758.1| unnamed protein product [Oryza sativa Japonica Group] &gt; gi|222619479|gb|EEE55611.1| hypothetical protein OsJ_03932 [Oryza sativa Japonica Group] ACN27868 unknown [Zea mays] 0.82 1092 1745 XP_002315131 laccase 90c [Populus 0.71 1093 1746 trichocarpa] &gt; gi|222864171|gb|EEF01302.1| laccase 90c [Populus trichocarpa] ABK92474 unknown [Populus 0.70 1094 1747 trichocarpa] XP_002312186 laccase 90a [Populus 0.70 1095 1748 trichocarpa] &gt; gi|222852006|gb|EEE89553.1| laccase 90a [Populus trichocarpa] 868-888 NP_001141205 hypothetical protein 1.00 1096 1749 LOC100273292 [Zea mays] &gt; gi|223944003|gb|ACN26085.1| unknown [Zea mays] ACF85713 unknown [Zea mays] 0.91 1097 1750 XP_002446013 hypothetical protein 0.84 1098 1751 SORBIDRAFT_06g000500 [Sorghum bicolor] &gt; gi|241937196|gb|EES10341.1| hypothetical protein SORBIDRAFT_06g000500 [Sorghum bicolor] XP_002446014 hypothetical protein 0.81 1099 1752 SORBIDRAFT_06g000510 [Sorghum bicolor] &gt; gi|241937197|gb|EES10342.1| hypothetical protein SORBIDRAFT_06g000510 [Sorghum bicolor] CAH67827 B0616E02-H0507E05.3 0.80 1100 1753 [Oryza sativa Indica Group] &gt; gi|125546940|gb|EAY92762.1| hypothetical protein OsI_14564 [Oryza sativa Indica Group] &gt; gi|125589087|gb|EAZ29437.1| hypothetical protein OsJ_13511 [Oryza sativa Japonica Group] BAJ88131 predicted protein [Hordeum 0.83 1101 1754 vulgare subsp. vulgare] 172-192 NP_001148700 caleosin related protein [Zea 1.00 1102 1755 mays] &gt; gi|195621488|gb|ACG32574.1| caleosin related protein [Zea mays] ACN31536 unknown [Zea mays] 0.99 1103 1756 ACG36841 caleosin related protein [Zea 0.83 1104 1757 mays] NP_001142815 hypothetical protein 0.82 1105 1758 LOC100275194 [Zea mays] &gt; gi|195610158|gb|ACG26909.1| hypothetical protein [Zea mays] XP_002454327 hypothetical protein 0.76 1106 1759 SORBIDRAFT_04g028730 [Sorghum bicolor] &gt; gi|241934158|gb|EES07303.1| hypothetical protein SORBIDRAFT_04g028730 [Sorghum bicolor] 782-802 XP_002465485 hypothetical protein 1.00 1107 1760 SORBIDRAFT_01g039690 [Sorghum bicolor] &gt; gi|241919339|gb|EER92483.1| hypothetical protein SORBIDRAFT_01g039690 [Sorghum bicolor] NP_001131665 hypothetical protein 0.92 1108 1761 LOC100193025 [Zea mays] &gt; gi|194692196|gb|ACF80182.1| unknown [Zea mays] NP_001049699 Os03g0273200 [Oryza sativa 0.82 1109 1762 Japonica Group] &gt; gi|122247226|sp|Q10ND7.1| LAC10_ORYSJ RecName: Full = Laccase-10; AltName: Full = Benzenediol:oxygen oxidoreductase 10; AltName: Full = Diphenol oxidase 10; AltName: Full = Urishiol oxidase 10; Flags: Precursor &gt; gi|108707435|gb|ABF95230.1| laccase, putative, expressed [Oryza sativa Japonica Group] &gt; gi|113548170|dbj|BAF11613.1| Os03g0273200 [Oryza sativa Japonica Group] &gt; gi|215704111|dbj|BAG92951.1| unnamed protein product [Oryza sativa Japonica Group] &gt; gi|215765805|dbj|BAG87502.1| unnamed protein product [Oryza sativa Japonica Group] &gt; gi|222624652|gb|EEE58784.1| hypothetical protein OsJ_10313 [Oryza sativa Japonica Group] XP_002531824 laccase, putative [Ricinus 0.73 1110 1763 communis] &gt; gi|223528520|gb|EEF30544.1| laccase, putative [Ricinus communis] XP_002278215 PREDICTED: hypothetical 0.73 1111 1764 protein [Vitis vinifera] CBI16199 unnamed protein product 0.72 1112 [Vitis vinifera] XP_002278232 PREDICTED: hypothetical 0.73 1113 1765 protein [Vitis vinifera] XP_002282823 PREDICTED: hypothetical 0.74 1114 1766 protein [Vitis vinifera] 943-963 NP_001183899 hypothetical protein 1.00 1115 1767 LOC100502492 [Zea mays] &gt; gi|223948465|gb|ACN28316.1| unknown [Zea mays] &gt; gi|238015342|gb|ACR38706.1| unknown [Zea mays] NP_001148479 L-ascorbate oxidase [Zea 0.90 1116 1768 mays] &gt; gi|195619672|gb|ACG31666.1| L-ascorbate oxidase precursor [Zea mays] XP_002456566 hypothetical protein 0.89 1117 1769 SORBIDRAFT_03g038550 [Sorghum bicolor] &gt; gi|241928541|gb|EES01686.1| hypothetical protein SORBIDRAFT_03g038550 [Sorghum bicolor] NP_001044679 Os01g0827300 [Oryza sativa 0.83 1118 1770 Japonica Group] &gt; gi|75331868|sp|Q941X2.1| LAC3_ORYSJ RecName: Full = Laccase-3; AltName: Full = Benzenediol:oxygen oxidoreductase 3; AltName: Full = Diphenol oxidase 3; AltName: Full = Urishiol oxidase 3; Flags: Precursor &gt; gi|15624045|dbj|BAB68098.1| putative laccase [Oryza sativa Japonica Group] &gt; gi|113534210|dbj|BAF06593.1| Os01g0827300 [Oryza sativa Japonica Group] &gt; gi|215701334|dbj|BAG92758.1| unnamed protein product [Oryza sativa Japonica Group] &gt; gi|222619479|gb|EEE55611.1| hypothetical protein OsJ_03932 [Oryza sativa Japonica Group] ACN27868 unknown [Zea mays] 0.80 1119 1771 XP_002315131 laccase 90c [Populus 0.70 1120 1772 trichocarpa] &gt; gi|222864171|gb|EEF01302.1| laccase 90c [Populus trichocarpa] CBI25418 unnamed protein product 0.71 1121 1773 [Vitis vinifera] XP_002273875 PREDICTED: hypothetical 0.71 1122 1774 protein [Vitis vinifera] 852-872 NP_001105875 putative laccase [Zea mays] 1.00 1123 1775 &gt; gi|84618781|emb|CAJ30500.1| putative laccase [Zea mays] ACN28855 unknown [Zea mays] 0.98 1124 1776 NP_001146658 hypothetical protein 0.94 1125 1777 LOC100280258 [Zea mays] &gt; gi|219888209|gb|ACL54479.1| unknown [Zea mays] XP_002458746 hypothetical protein 0.91 1126 1778 SORBIDRAFT_03g039520 [Sorghum bicolor] &gt; gi|241930721|gb|EES03866.1| hypothetical protein SORBIDRAFT_03g039520 [Sorghum bicolor] NP_001044772 Os01g0842400 [Oryza sativa 0.80 1127 1779 Japonica Group] &gt; gi|75321217|sp|Q5N9X2.1| LAC4_ORYSJ RecName: Full = Laccase-4; AltName: Full = Benzenediol:oxygen oxidoreductase 4; AltName: Full = Diphenol oxidase 4; AltName: Full = Urishiol oxidase 4; Flags: Precursor &gt; gi|56784239|dbj|BAD81734.1| putative laccase LAC5-6 [Oryza sativa Japonica Group] &gt; gi|113534303|dbj|BAF06686.1| Os01g0842400 [Oryza sativa Japonica Group] &gt; gi|215697155|dbj|BAG91149.1| unnamed protein product [Oryza sativa Japonica Group] EAZ14115 hypothetical protein 0.80 1128 OsJ_04039 [Oryza sativa Japonica Group] EEC71777 hypothetical protein 0.80 1129 OsI_04389 [Oryza sativa Indica Group] AAC04576 putative high-pI laccase 0.79 1130 1780 [Oryza sativa Japonica Group] BAJ99773 predicted protein [Hordeum 0.77 1131 1781 vulgare subsp. vulgare] XP_002458747 hypothetical protein 0.78 1132 1782 SORBIDRAFT_03g039530 [Sorghum bicolor] &gt; gi|241930722|gb|EES03867.1| hypothetical protein SORBIDRAFT_03g039530 [Sorghum bicolor] 801-821 XP_002441216 hypothetical protein 1.00 1133 1783 SORBIDRAFT_09g022460 [Sorghum bicolor] &gt; gi|241946501|gb|EES19646.1| hypothetical protein SORBIDRAFT_09g022460 [Sorghum bicolor] NP_001105874 putative laccase [Zea mays] 0.89 1134 1784 &gt; gi|84618777|emb|CAJ30498.1| putative laccase [Zea mays] Q0DHL5 RecName: Full = Putative 0.88 1135 laccase-11; AltName: Full = Benzenediol:oxygen oxidoreductase 11; AltName: Full = Diphenol ocidase 11; AltName: Full = Urishiol oxidase 11 &gt; gi|222631843|gb|EEE63975.1| hypothetical protein OsJ_18801 [Oryza sativa Japonica Group] NP_001055744 Os05g0458300 [Oryza sativa 0.81 1136 1785 Japonica Group] &gt; gi|113579295|dbj|BAF17658.1| Os05g0458300 [Oryza sativa Japonica Group] XP_002456622 hypothetical protein 0.76 1137 1786 SORBIDRAFT_03g039570 [Sorghum bicolor] &gt; gi|241928597|gb|EES01742.1| hypothetical protein SORBIDRAFT_03g039570 [Sorghum bicolor] ACG47007 L-ascorbate oxidase precursor 0.76 1138 1787 [Zea mays] NP_001105921 putative laccase [Zea mays] 0.74 1139 1788 &gt; gi|84618783|emb|CAJ30497.1| putative laccase [Zea mays] 673-693 NP_001168720 hypothetical protein 1.00 1140 1789 LOC100382512 [Zea mays] &gt; gi|223950395|gb|ACN29281.1| unknown [Zea mays] XP_002448765 hypothetical protein 0.98 1141 1790 SORBIDRAFT_06g032800 [Sorghum bicolor] &gt; gi|241939948|gb|EES13093.1| hypothetical protein SORBIDRAFT_06g032800 [Sorghum bicolor] NP_001131374 hypothetical protein 0.98 1142 1791 LOC100192699 [Zea mays] &gt; gi|195620734|gb|ACG32197.1| oligosaccharyl transferase STT3 subunit [Zea mays] NP_001054248 Os04g0675500 [Oryza sativa 0.96 1143 1792 Japonica Group] &gt; gi|38344929|emb|CAE03245.2| OSJNBa0018M05.20 [Oryza sativa Japonica Group] &gt; gi|90399055|emb|CAJ86104.1| H0103C06.8 [Oryza sativa Indica Group] &gt; gi|113565819|dbj|BAF16162.1| Os04g0675500 [Oryza sativa Japonica Group] &gt; gi|125550210|gb|EAY96032.1| hypothetical protein OsI_17905 [Oryza sativa Indica Group] &gt; gi|125592048|gb|EAZ32398.1| hypothetical protein OsJ_16609 [Oryza sativa Japonica Group] &gt; gi|215708677|dbj|BAG93946.1| unnamed protein product [Oryza sativa Japonica Group] BAJ87792 predicted protein [Hordeum 0.94 1144 1793 vulgare subsp. vulgare] XP_002269119 PREDICTED: hypothetical 0.86 1145 1794 protein [Vitis vinifera] CBG76274 OO_Ba0005L10- 0.93 1146 1795 OO_Ba0081K17.25 [Oryza officinalis] XP_002318345 predicted protein [Populus 0.86 1147 1796 trichocarpa] &gt; gi|222859018|gb|EEE96565.1| predicted protein [Populus trichocarpa] XP_002891078 hypothetical protein 0.86 1148 1797 ARALYDRAFT_891007 [Arabidopsis lyrata subsp. lyrata] &gt; gi|297336920|gb|EFH67337.1| hypothetical protein ARALYDRAFT_891007 [Arabidopsis lyrata subsp. lyrata] XP_002329687 predicted protein [Populus 0.85 1149 1798 trichocarpa] &gt; gi|222870595|gb|EEF07726.1| predicted protein [Populus trichocarpa] 1539-1559 XP_002462694 hypothetical protein 1.00 1150 1799 SORBIDRAFT_02g030360 [Sorghum bicolor] &gt; gi|241926071|gb|EER99215.1| hypothetical protein SORBIDRAFT_02g030360 [Sorghum bicolor] NP_001168360 hypothetical protein 0.92 1151 1800 LOC100382128 [Zea mays] &gt; gi|223947749|gb|ACN27958.1| unknown [Zea mays] NP_001063714 Os09g0524300 [Oryza sativa 0.80 1152 1801 Japonica Group] &gt; gi|52076031|dbj|BAD46484.1| ethionine resistance protein-like [Oryza sativa Japonica Group] &gt; gi|255679074|dbj|BAF25628.2| Os09g0524300 [Oryza sativa Japonica Group] ABG73448 MATE efflux family protein 0.80 1153 1802 [Oryza brachyantha] EAZ09807 hypothetical protein 0.80 1154 OsI_32095 [Oryza sativa Indica Group] XP_002444635 hypothetical protein 0.73 1155 1803 SORBIDRAFT_07g025190 [Sorghum bicolor] &gt; gi|241940985|gb|EES14130.1| hypothetical protein SORBIDRAFT_07g025190 [Sorghum bicolor] BAD09230 putative ripening regulated 0.70 1156 1804 protein DDTFR18 [Oryza sativa Japonica Group] 1198-1218 NP_001146658 hypothetical protein 1.00 1157 1805 LOC100280258 [Zea mays] &gt; gi|219888209|gb|ACL54479.1| unknown [Zea mays] NP_001105875 putative laccase [Zea mays] 0.95 1158 1806 &gt; gi|84618781|emb|CAJ30500.1| putative laccase [Zea mays] ACN28855 unknown [Zea mays] 0.92 1159 1807 XP_002458746 hypothetical protein 0.91 1160 1808 SORBIDRAFT_03g039520 [Sorghum bicolor] &gt; gi|241930721|gb|EES03866.1| hypothetical protein SORBIDRAFT_03g039520 [Sorghum bicolor] NP_001044772 Os01g0842400 [Oryza sativa 0.80 1161 1809 Japonica Group] &gt; gi|75321217|sp|Q5N9X2.1| LAC4_ORYSJ RecName: Full = Laccase-4; AltName: Full = Benzenediol:oxygen oxidoreductase 4; AltName: Full = Diphenol oxidase 4; AltName: Full = Urishiol oxidase 4; Flags: Precursor &gt; gi|56784239|dbj|BAD81734.1| putative laccase LAC5-6 [Oryza sativa Japonica Group] &gt; gi|113534303|dbj|BAF06686.1| Os01g0842400 [Oryza sativa Japonica Group] &gt; gi|215697155|dbj|BAG91149.1| unnamed protein product [Oryza sativa Japonica Group] EAZ14115 hypothetical protein 0.80 1162 OsJ_04039 [Oryza sativa Japonica Group] EEC71777 hypothetical protein 0.80 1163 OsI_04389 [Oryza sativa Indica Group] BAJ99773 predicted protein [Hordeum 0.78 1164 1810 vulgare subsp. vulgare] AAC04576 putative high-pI laccase 0.78 1165 1811 [Oryza sativa Japonica Group] XP_002458747 hypothetical protein 0.77 1166 1812 SORBIDRAFT_03g039530 [Sorghum bicolor] &gt; gi|241930722|gb|EES03867.1| hypothetical protein SORBIDRAFT_03g039530 [Sorghum bicolor] 934-954 ACN27868 unknown [Zea mays] 1.00 1167 1813 NP_001148479 L-ascorbate oxidase [Zea 0.94 1168 1814 mays] &gt; gi|195619672|gb|ACG31666.1| L-ascorbate oxidase precursor [Zea mays] XP_002456566 hypothetical protein 0.90 1169 1815 SORBIDRAFT_03g038550 [Sorghum bicolor] &gt; gi|241928541|gb|EES01686.1| hypothetical protein SORBIDRAFT_03g038550 [Sorghum bicolor] NP_001183899 hypothetical protein 0.88 1170 1816 LOC100502492 [Zea mays] &gt; gi|223948465|gb|ACN28316.1| unknown [Zea mays] &gt; gi|238015342|gb|ACR38706.1| unknown [Zea mays] NP_001044679 Os01g0827300 [Oryza sativa 0.81 1171 1817 Japonica Group] &gt; gi|75331868|sp|Q941X2.1| LAC3_ORYSJ RecName: Full = Laccase-3; AltName: Full = Benzenediol:oxygen oxidoreductase 3; AltName: Full = Diphenol oxidase 3; AltName: Full = Urishiol oxidase 3; Flags: Precursor &gt; gi|15624045|dbj|BAB68098.1| putative laccase [Oryza sativa Japonica Group] &gt; gi|113534210|dbj|BAF06593.1| Os01g0827300 [Oryza sativa Japonica Group] &gt; gi|215701334|dbj|BAG92758.1| unnamed protein product [Oryza sativa Japonica Group] &gt; gi|222619479|gb|EEE55611.1| hypothetical protein OsJ_03932 [Oryza sativa Japonica Group] Predicted 1620-1643 NP_001137083 hypothetical protein 1.00 1172 1818 folded LOC100217256 [Zea mays] 24-nts- &gt; gi|194698278|gb|ACF83223.1| long seq unknown [Zea mays] 52739 XP_002446946 hypothetical protein 0.75 1173 1819 SORBIDRAFT_06g025600 [Sorghum bicolor] &gt; gi|241938129|gb|EES11274.1| hypothetical protein SORBIDRAFT_06g025600 [Sorghum bicolor] Predicted 334-354 XP_002459032 hypothetical protein 1.00 1174 1820 zma SORBIDRAFT_03g044830 mir [Sorghum bicolor] 49816 &gt; gi|241931007|gb|EES04152.1| hypothetical protein SORBIDRAFT_03g044830 [Sorghum bicolor] NP_001131974 hypothetical protein 0.90 1175 1821 LOC100193372 [Zea mays] &gt; gi|194693076|gb|ACF80622.1| unknown [Zea mays] BAJ85237 predicted protein [Hordeum 0.84 1176 1822 vulgare subsp. vulgare] &gt; gi|326511587|dbj|BAJ91938.1| predicted protein [Hordeum vulgare subsp. vulgare] NP_001045294 Os01g0931100 [Oryza sativa 0.85 1177 1823 Japonica Group] &gt; gi|57899654|dbj|BAD87323.1| unknown protein [Oryza sativa Japonica Group] &gt; gi|57900117|dbj|BAD88179.1| unknown protein [Oryza sativa Japonica Group] &gt; gi|113534825|dbj|BAF07208.1| Os01g0931100 [Oryza sativa Japonica Group] &gt; gi|215697092|dbj|BAG91086.1| unnamed protein product [Oryza sativa Japonica Group] 116-136 CAE04743 OSJNBb0060E08.6 [Oryza 1.00 1178 1824 sativa Japonica Group] &gt; gi|116309791|emb|CAH66831.1| OSIGBa0148A10.8 [Oryza sativa Indica Group] NP_001053941 0s04g0625800 [Oryza sativa 1.00 1179 1825 Japonica Group] &gt; gi|113565512|dbj|BAF15855.1| Os04g0625800 [Oryza sativa Japonica Group] &gt; gi|215767966|dbj|BAH00195.1| unnamed protein product [Oryza sativa Japonica Group] EAY95645 hypothetical protein 1.00 1180 OsI_17510 [Oryza sativa Indica Group] NP_001131852 hypothetical protein 0.79 1181 1826 LOC100193230 [Zea mays] &gt; gi|194692726|gb|ACF80447.1| unknown [Zea mays] BAK03410 predicted protein [Hordeum 0.81 1182 1827 vulgare subsp. vulgare] &gt; gi|326525779|dbj|BAJ88936.1| predicted protein [Hordeum vulgare subsp. vulgare] XP_002447134 hypothetical protein 0.78 1183 1828 SORBIDRAFT_06g029180 [Sorghum bicolor] &gt; gi|241938317|gb|EES11462.1| hypothetical protein SORBIDRAFT_06g029180 [Sorghum bicolor] NP_001168401 hypothetical protein 0.77 1184 1829 LOC100382170 [Zea mays] &gt; gi|223948043|gb|ACN28105.1| unknown [Zea mays] ACF85557 unknown [Zea mays] 0.74 1185 1830 NP_001141359 hypothetical protein 0.74 1186 1831 LOC100273450 [Zea mays] &gt; gi|194704160|gb|ACF86164.1| unknown [Zea mays]  81-101 XP_002463519 hypothetical protein 1.00 1187 1832 SORBIDRAFT_01g001270 [Sorghum bicolor] &gt; gi|241917373|gb|EER90517.1| hypothetical protein SORBIDRAFT_01g001270 [Sorghum bicolor] BAK01513 predicted protein [Hordeum 0.86 1188 1833 vulgare subsp. vulgare] NP_001051898 Os03g0849600 [Oryza sativa 0.85 1189 1834 Japonica Group] &gt; gi|28269403|gb|AAO37946.1| putative conserved oligomeric Golgi complex component [Oryza sativa Japonica Group] &gt; gi|108712114|gb|ABF99909.1| brefeldin A-sensitive Golgi protein, putative, expressed [Oryza sativa Japonica Group] &gt; gi|113550369|dbj|BAF13812.1| Os03g0849600 [Oryza sativa Japonica Group] &gt; gi|125588645|gb|EAZ29309.1| hypothetical protein OsJ_13370 [Oryza sativa Japonica Group] EEC76534 hypothetical protein 0.85 1190 OsI_14326 [Oryza sativa Indica Group] 322-342 NP_001168448 hypothetical protein 1.00 1191 1835 LOC100382221 [Zea mays] &gt; gi|223948365|gb|ACN28266.1| unknown [Zea mays]  98-118 BAD05744 putative RNA Binding Protein 1.00 1192 1836 45 [Oryza sativa Japonica Group] &gt; gi|40253847|dbj|BAD05783.1| putative RNA Binding Protein 45 [Oryza sativa Japonica Group] EEE68172 hypothetical protein 0.75 1193 OSJ_26296 [Oryza sativa Japonica Group] ACF85557 unknown [Zea mays] 0.78 1194 1837 NP_001141359 hypothetical protein 0.78 1195 1838 LOC100273450 [Zea mays] &gt; gi|194704160|gb|ACF86164.1| unknown [Zea mays] aqc- 955-975 AAX83875 teosinte glume architecture 1 1.00 1196 1839 miR529 [Zea mays subsp. mays] AAX83872 teosinte glume architecture 1 0.99 1197 1840 [Zea mays subsp. mays] AAX83873 teosinte glume architecture 1 0.99 1198 1841 [Zea mays subsp. mays] &gt; gi|62467440|gb|AAX83874.1| teosinte glume architecture 1 [Zea mays subsp. mays] XP_002445815 hypothetical protein 0.80 1199 1842 SORBIDRAFT_07g026220 [Sorghum bicolor] &gt; gi|241942165|gb|EES15310.1| hypothetical protein SORBIDRAFT_07g026220 [Sorghum bicolor] 831-851 ADX60108 SBP transcription factor [Zea 1.00 1200 1843 mays] XP_002446904 hypothetical protein 0.72 1201 1844 SORBIDRAFT_06g024630 [Sorghum bicolor] &gt; gi|241938087|gb|EES11232.1| hypothetical protein SORBIDRAFT_06g024630 [Sorghum bicolor] 882-902 ACN30570 unknown [Zea mays] 1.00 1202 1845 &gt; gi|223973927|gb|ACN31151.1| unknown [Zea mays] &gt; gi|323388595|gb|ADX60102.1| SBP transcription factor [Zea mays] NP_001145445 hypothetical protein 0.98 1203 1846 LOC100278824 [Zea mays] &gt; gi|195656399|gb|ACG47667.1| hypothetical protein [Zea mays] XP_002450775 hypothetical protein 0.87 1204 1847 SORBIDRAFT_05g017510 [Sorghum bicolor] &gt; gi|241936618|gb|EES09763.1| hypothetical protein SORBIDRAFT_05g017510 [Sorghum bicolor] 656-676 NP_001137049 hypothetical protein 1.00 1205 1848 LOC100217221 [Zea mays] &gt; gi|194698154|gb|ACF83161.1| unknown [Zea mays] 1248-1268 XP_002447211 hypothetical protein 1.00 1206 1849 SORBIDRAFT_06g030520 [Sorghum bicolor] &gt; gi|241938394|gb|EES11539.1| hypothetical protein SORBIDRAFT_06g030520 [Sorghum bicolor] NP_001132831 hypothetical protein 0.90 1207 1850 LOC100194321 [Zea mays] &gt; gi|194695516|gb|ACF81842.1| unknown [Zea mays] NP_001054060 Os04g0644700 [Oryza sativa 0.80 1208 1851 Japonica Group] &gt; gi|148886836|sp|P0C541.1| COPE2_ORYSJ RecName: Full = Coatomer subunit epsilon-2; AltName: Full = Epsilon-coat protein 2; Short = Epsilon-COP 2 &gt; gi|38344895|emb|CAD41918.2| OSJNBa0033G05.19 [Oryza sativa Japonica Group] &gt; gi|113565631|dbj|BAF15974.1| Os04g0644700 [Oryza sativa Japonica Group] &gt; gi|125591839|gb|EAZ32189.1| hypothetical protein OsJ_16395 [Oryza sativa Japonica Group] A2XY73 RecName: Full = Coatomer 0.80 1209 subunit epsilon-2; AltName: Full = Epsilon-coat protein 2; Short = Epsilon-COP 2 &gt; gi|90399097|emb|CAJ86157.1| H0413E07.10 [Oryza sativa Indica Group] EAY95783 hypothetical protein 0.80 1210 OsI_17658 [Oryza sativa Indica Group]  82-102 ACR34442 unknown [Zea mays] 1.00 1211 1852  82-102 NP_00145733 hypothetical protein 1.00 1212 1853 LOC100279240 [Zea mays] &gt; gi|219884215|gb|ACL52482.1| unknown [Zea mays] XP_002460490 hypothetical protein 0.87 1213 1854 SORBIDRAFT_02g029300 [Sorghum bicolor] &gt; gi|241923867|gb|EER97011.1| hypothetical protein SORBIDRAFT_02g029300 [Sorghum bicolor] NP_001063612 Os09g0507100 [Oryza sativa 0.71 1214 1855 Japonica Group] &gt; gi|122234416|sp|Q0JOK1.1| SPL18_ORYSJ RecName: Full = Squamosa promoter- binding-like protein 18 &gt; gi|113631845|dbj|BAF25526.1| Os09g0507100 [Oryza sativa Japonica Group]  996-1016 ACF86782 unknown [Zea mays] 1.00 1215 1856 &gt; gi|323388573|gb|ADX60091.1| SBP transcription factor [Zea mays] ACG45113 squamosa promoter-binding- 1.00 1216 1857 like protein 9 [Zea mays] XP_002462571 hypothetical protein 0.83 1217 1858 SORBIDRAFT_02g028420 [Sorghum bicolor] &gt; gi|241925948|gb|EER99092.1| hypothetical protein SORBIDRAFT_02g028420 [Sorghum bicolor] NP_001136945 hypothetical protein 0.76 1218 1859 LOC100217104 [Zea mays] &gt; gi|194697718|gb|ACF82943.1| unknown [Zea mays] 1120-1140 ACL52941 unknown [Zea mays] 1.00 1219 1860 XP_002444771 hypothetical protein 0.76 1220 1861 SORBIDRAFT_07g027740 [Sorghum bicolor] &gt; gi|241941121|gb|EES14266.1| hypothetical protein SORBIDRAFT_07g027740 [Sorghum bicolor] 1348-1368 XP_002438971 hypothetical protein 1.00 1221 1862 SORBIDRAFT_10g029190 [Sorghum bicolor] &gt; gi|241917194|gb|EER90338.1| hypothetical protein SORBIDRAFT_10g029190 [Sorghum bicolor] NP_001149534 squamosa promoter-binding- 0.88 1222 1863 like protein 11 [Zea mays] &gt; gi|195627850|gb|ACG35755.1| squamosa promoter- binding-like protein 11 [Zea mays] &gt; gi|195644948|gb|ACG41942.1| squamosa promoter- binding-like protein 11 [Zea mays] 221-241 XP_002447219 hypothetical protein 1.00 1223 1864 SORBIDRAFT_06g030650 [Sorghum bicolor] &gt; gi|241938402|gb|EES11547.1| hypothetical protein SORBIDRAFT_06g030650 [Sorghum bicolor] NP_001169344 hypothetical protein 0.92 1224 1865 LOC100383211 [Zea mays] &gt; gi|224028835|gb|ACN33493.1| unknown [Zea mays] NP_001147890 LOC100281500 [Zea mays] 0.90 1225 1866 &gt; gi|195614420|gb|ACG29040.1| zinc finger, C3HC4 type family protein [Zea mays] CAD41707 OSJNBa0010D21.9 [Oryza 0.74 1226 1867 sativa Japonica Group] &gt; gi|125549990|gb|EAY95812.1| hypothetical protein OsI_17683 [Oryza sativa Indica Group] &gt; gi|125591860|gb|EAZ32210.1| hypothetical protein OsJ_16417 [Oryza sativa Japonica Group] &gt; gi|215701084|dbj|BAG92508.1| unnamed protein product [Oryza sativa Japonica Group] 973-993 NP_001136945 hypothetical protein 1.00 1227 1868 LOC100217104 [Zea mays] &gt; gi|194697718|gb|ACF82943.1| unknown [Zea mays] XP_002462571 hypothetical protein 0.82 1228 1869 SORBIDRAFT_02g028420 [Sorghum bicolor] &gt; gi|241925948|gb|EER99092.1| hypothetical protein SORBIDRAFT_02g028420 [Sorghum bicolor] ACF86782 unknown [Zea mays] 0.76 1229 1870 &gt; gi|323388573|gb|ADX60091.1| SBP transcription factor [Zea mays] ACG45113 squamosa promoter-binding- 0.76 1230 1871 like protein 9 [Zea mays] 558-578 CAB56631 SBP-domain protein 5 [Zea 1.00 1231 1872 mays] XP_002444771 hypothetical protein 0.85 1232 1873 SORBIDRAFT_07g027740 [Sorghum bicolor] &gt; gi|241941121|gb|EES14266.1| hypothetical protein SORBIDRAFT_07g027740 [Sorghum bicolor] ACL52941 unknown [Zea mays] 0.78 1233 1874 1410-1430 NP_001152658 MTA/SAH nucleosidase [Zea 1.00 1234 1875 mays] &gt; gi|195658647|gb|ACG48791.1| MTA/SAH nucleosidase [Zea mays] &gt; gi|223973627|gb|ACN31001.1| unknown [Zea mays] ACF83838 unknown [Zea mays] 0.88 1235 1876 ACG39594 MTA/SAH nucleosidase [Zea 0.88 1236 1877 mays] XP_002445813 hypothetical protein 0.88 1237 1878 SORBIDRAFT_07g026190 [Sorghum bicolor] &gt; gi|241942163|gb|EES15308.1| hypothetical protein SORBIDRAFT_07g026190 [Sorghum bicolor] ACN31483 unknown [Zea mays] 0.90 1238 1879 NP_001056592 Os06g0112200 [Oryza sativa 0.80 1239 1880 Japonica Group] &gt; gi|7363290|dbj|BAA93034.1| methylthioadenosine/S- adenosyl homocysteine nucleosidase [Oryza sativa Japonica Group] &gt; gi|32352128|dbj|BAC78557.1| hypothetical protein [Oryza sativa Japonica Group] &gt; gi|113594632|dbj|BAF18506.1| Os06g0112200 [Oryza sativa Japonica Group] &gt; gi|125595804|gb|EAZ35584.1| hypothetical protein OsJ_19870 [Oryza sativa Japonica Group] &gt; gi|215694661|dbj|BAG89852.1| unnamed protein product [Oryza sativa Japonica Group] &gt; gi|215740802|dbj|BAG96958.1| unnamed protein product [Oryza sativa Japonica Group] AAL58883 methylthioadenosine/S- 0.79 1240 1881 adenosyl homocysteine nucleosidase [Oryza sativa] ABR25495 mta/sah nucleosidase [Oryza 0.79 1241 1882 sativa Indica Group] BAK03317 predicted protein [Hordeum 0.78 1242 1883 vulgare subsp. vulgare] &gt; gi|326534118|dbj|BAJ89409.1| predicted protein [Hordeum vulgare subsp. vulgare] EAY99382 hypothetical protein 0.78 1243 OsI_21350 [Oryza sativa Indica Group] 1197-1217 AAX83872 teosinte glume architecture 1 1.00 1244 1884 [Zea mays subsp. mays] AAX83875 teosinte glume architecture 1 0.98 1245 1885 [Zea mays subsp. mays] AAX83873 teosinte glume architecture 1 0.99 1246 1886 [Zea mays subsp. mays] &gt; gi|62467440|gb|AAX83874.1| teosinte glume architecture 1 [Zea mays subsp. mays] XP_002445815 hypothetical protein 0.80 1247 1887 SORBIDRAFT_07g026220 [Sorghum bicolor] &gt; gi|241942165|gb|EES15310.1| hypothetical protein SORBIDRAFT_07g026220 [Sorghum bicolor] BAK05794 predicted protein [Hordeum 0.63 1248 1888 vulgare subsp. vulgare] Predicted 748-771 NP_001143705 hypothetical protein 1.00 1281 1911 folded LOC100276442 [Zea mays] 24-nts- &gt; gi|195625088|gb|ACG34374.1| long seq hypothetical protein [Zea 52792 mays] ACF83056 unknown [Zea mays] 0.95 1282 1912 1078-1101 ACF83056 unknown [Zea mays] 1.00 1283 1913 NP_001143705 hypothetical protein 0.88 1284 1914 LOC100276442 [Zea mays] &gt; gi|195625088|gb|ACG34374.1| hypothetical protein [Zea mays] Predicted 278-301 XP_002458944 hypothetical protein 1.00 1285 1915 folded SORBIDRAFT_03g043140 24-nts- [Sorghum bicolor] long seq &gt; gi|241930919|gb|EES04064.1| 51757 hypothetical protein SORBIDRAFT_03g043140 [Sorghum bicolor] ACF79162 unknown [Zea mays] 0.98 1286 1916 &gt; gi|194703646|gb|ACF85907.1| unknown [Zea mays] NP_001105336 fructose-bisphosphate 0.98 1287 1917 aldolase, cytoplasmic isozyme [Zea mays] &gt; gi|113621|sp|P08440.1|ALF_MAIZE RecName: Full = Fructose-bisphosphate aldolase, cytoplasmic isozyme &gt; gi|168420|gb|AAA33435.1| aldolase [Zea mays] &gt; gi|295850|emb|CAA31366.1| fructose bisphosphate aldolase [Zea mays] &gt; gi|225624|prf||1307278A cytoplasmic aldolase NP_001150049 fructose-bisphosphate aldolase 0.98 1288 1918 cytoplasmic isozyme [Zea mays] &gt; gi|194704898|gb|ACF86533.1| unknown [Zea mays] &gt; gi|195636310|gb|ACG37623.1| fructose-bisphosphate aldolase cytoplasmic isozyme [Zea mays] XP_002453822 hypothetical protein 0.95 1289 1919 SORBIDRAFT_04g019020 [Sorghum bicolor] &gt; gi|241933653|gb|EES06798.1| hypothetical protein SORBIDRAFT_04g019020 [Sorghum bicolor] P17784 RecName: Full = Fructose- 0.94 1290 bisphosphate aldolase cytoplasmic isozyme; AltName: Full = Gravity- specific protein GSC 233 &gt; gi|41398198|gb|AAS05825.1| fructose 1,6-bisphosphate aldolase [Oryza sativa Japonica Group] &gt; gi|50878379|gb|AAT85154.1| putative fructose- bisphosphate aldolase [Oryza sativa Japonica Group] &gt; gi|50878433|gb|AAT85207.1| putative fructose- bisphosphate aldolase [Oryza sativa Japonica Group] &gt; gi|169244417|gb|ACA50482.1| fructose-bisphosphate aldolase [Oryza sativa Japonica Group] &gt; gi|306415953|gb|ADM86851.1| fructose-bisphosphate aldolase [Oryza sativa Japonica Group] BAI59774 aldolase C-1 [Phyllostachys 0.94 1291 1920 edulis] EAY94426 hypothetical protein 0.93 1292 OsI_16195 [Oryza sativa Indica Group] CAA37290 unnamed protein product 0.93 1293 1921 [Oryza sativa Japonica Group] ABG65931 Fructose-bisphosphate 0.92 1294 1922 aldolase, cytoplasmic isozyme, putative, expressed [Oryza sativa Japonica Group] &gt; gi|125574121|gb|EAZ15405.1| hypothetical protein OsJ_30817 [Oryza sativa Japonica Group] &gt; gi|215701125|dbj|BAG92549.1| unnamed protein product [Oryza sativa Japonica Group] &gt; gi|215708712|dbj|BAG93981.1| unnamed protein product [Oryza sativa Japonica Group] 686-709 NP_001145778 hypothetical protein 1.00 1295 1923 LOC100279285 [Zea mays] &gt; gi|219884395|gb|ACL52572.1| unknown [Zea mays] ACF87373 unknown [Zea mays] 1.00 1296 1924 ACG25535 ribosomal RNA apurinic site 0.99 1297 1925 specific lyase [Zea mays] XP_002455890 hypothetical protein 0.79 1298 1926 SORBIDRAFT_03g026880 [Sorghum bicolor] &gt; gi|241927865|gb|EES01010.1| hypothetical protein SORBIDRAFT_03g026880 [Sorghum bicolor] 365-388 ACR36335 unknown [Zea mays] 1.00 1299 1927 ACR35742 unknown [Zea mays] 0.75 1300 1928 512-535 XP_002458102 hypothetical protein 1.00 1301 1929 SORBIDRAFT_03g026990 [Sorghum bicolor] &gt; gi|241930077|gb|EE503222.1| hypothetical protein SORBIDRAFT_03g026990 [Sorghum bicolor] NP_001151540 hydrogen-transporting ATP 0.93 1302 1930 synthase, rotational mechanism [Zea mays] &gt; gi|195605790|gb|ACG24725.1| hydrogen-transporting ATP synthase, rotational mechanism [Zea mays] &gt; gi|195608344|gb|ACG26002.1| hydrogen-transporting ATP synthase, rotational mechanism [Zea mays] &gt; gi|195647528|gb|ACG43232.1| hydrogen-transporting ATP synthase, rotational mechanism [Zea mays] &gt; gi|195658703|gb|ACG48819.1| hydrogen-transporting ATP synthase, rotational mechanism [Zea mays] ACF82609 unknown [Zea mays] 0.93 1303 1931 NP_001149611 LOC100283237 [Zea mays] 0.93 1304 1932 &gt; gi|194696248|gb|ACF82208.1| unknown [Zea mays] &gt; gi|195605550|gb|ACG24605.1| hydrogen-transporting ATP synthase, rotational mechanism [Zea mays] &gt; gi|195619622|gb|ACG31641.1| hydrogen-transporting ATP synthase, rotational mechanism [Zea mays] &gt; gi|195628500|gb|ACG36080.1| hydrogen-transporting ATP synthase, rotational mechanism [Zea mays] &gt; gi|195637444|gb|ACG38190.1| hydrogen-transporting ATP synthase, rotational mechanism [Zea mays] &gt; gi|195644406|gb|ACG41671.1| hydrogen-transporting ATP synthase, rotational mechanism [Zea mays] BAJ87122 predicted protein [Hordeum 0.89 1305 1933 vulgare subsp. vulgare] NP_001043488 Os01g0600000 [Oryza sativa 0.88 1306 1934 Japonica Group] &gt; gi|113533019|dbj|BAF05402.1| Os01g0600000 [Oryza sativa Japonica Group] &gt; gi|125571065|gb|EAZ12580.1| hypothetical protein OsJ_02485 [Oryza sativa Japonica Group] &gt; gi|215768142|dbj|BAH00371.1| unnamed protein product [Oryza sativa Japonica Group] &gt; gi|218188591|gb|EEC71018.1| hypothetical protein OsI_02711 [Oryza sativa Indica Group] ABF70110 mitochondrial ATP synthase g 0.78 1307 1935 subunit family protein [Musabalbisiana] ABA40451 unknown [Solanum 0.72 1308 1936 tuberosum] NP_001056144 Os05g0533800 [Oryza sativa 0.73 1309 1937 Japonica Group] &gt; gi|48843828|gb|AAT47087.1| unknown protein [Oryza sativa Japonica Group] &gt; gi|113579695|dbj|BAF18058.1| Os05g0533800 [Oryza sativa Japonica Group] &gt; gi|222632347|gb|EEE64479.1| hypothetical protein OsJ_19329 [Oryza sativa Japonica Group] BAJ90534 predicted protein [Hordeum 0.74 1310 1938 vulgare subsp. vulgare] ath- 742-763 ACN26323 unknown [Zea mays] 1.00 1389 2005 miR2936 &gt; gi|223944533|gb|ACN26350.1| unknown [Zea mays] BAC01259 glycosylphosphatidylinositol 0.78 1390 2006 anchor attachment 1-like [Oryza sativa Japonica Group] &gt; gi|125571593|gb|EAZ13108.1| hypothetical protein OsJ_03027 [Oryza sativa Japonica Group] EAY75389 hypothetical protein 0.78 1391 OsI_03287 [Oryza sativa Indica Group] Table 6: Provided are the target Genes of miRNAs Associated with Increased NUE (Table 2) along with their GenBank Accession numbers and sequence identifiers (SEQ ID NO:). “bind” = binding; “pos” = position; “hom” = homologue; “p.p.” = polypeptide; “p.n.” = polynucleotide.

TABLE 7 Target genes of up regulated siRNA sequences associated with NUE (Table 3) p.n. Mir Hom. p.p. SEQ Mir Bind. NCBI SEQ ID ID name Pos. Accession Annotation/organism Identity NO: NO: Predicted 1180-1201 XP_002439783 hypothetical protein 1.00 2007 2437 siRNA SORBIDRAFT_09g019980 59952 [Sorghum bicolor] &gt; gi|241945068|gb|EES18213.1| hypothetical protein SORBIDRAFT_09g019980 [Sorghum bicolor] NP_001140772 hypothetical protein 0.87 2008 2438 LOC100272847 [Zea mays] &gt; gi|194701020|gb|ACF84594.1| unknown [Zea mays] ACG40649 hypothetical protein [Zea mays] 0.87 2009 2439 NP_001140587 hypothetical protein 0.85 2010 2440 LOC100272657 [Zea mays] &gt; gi|194700088|gb|ACF84128.1| unknown [Zea mays] ACG28586 hypothetical protein [Zea mays] 0.83 2011 2441 EEC79208 hypothetical protein OsI_19925 0.73 2012 [Oryza sativa Indica Group] 2775-2796 NP_001146628 hypothetical protein 1.00 2013 2442 LOC100280226 [Zea mays] &gt; gi|219888087|gb|ACL54418.1| unknown [Zea mays] EEE65008 hypothetical protein OsJ_19956 0.71 2014 [Oryza sativa Japonica Group] BAA90807 putative SEU1 protein [Oryza 0.71 2015 2443 sativa Japonica Group] &gt; gi|215704489|dbj|BAG93923.1| unnamed protein product [Oryza sativa Japonica Group] Predicted 220-238 ACR33998 unknown [Zea mays] 1.00 2016 2444 siRNA 59626 NP_001141472 hypothetical protein 1.00 2017 2445 LOC100273582 [Zea mays] &gt; gi|194704716|gb|ACF86442.1| unknown [Zea mays] 1435-1453 XP_002446603 hypothetical protein 1.00 2018 2446 SORBIDRAFT_06g018770 [Sorghum bicolor] &gt; gi|241937786|gb|EES10931.1| hypothetical protein SORBIDRAFT_06g018770 [Sorghum bicolor] NP_001159223 hypothetical protein 0.92 2019 2447 LOC100304309 [Zea mays] &gt; gi|223942807|gb|ACN25487.1| unknown [Zea mays] BAJ94860 predicted protein [Hordeum 0.79 2020 2448 vulgare subsp. vulgare] CAE03375 OSJNBa0036B21.17 [Oryza 0.80 2021 sativa Japonica Group] EEE61116 hypothetical protein OsJ_15040 0.80 2022 [Oryza sativa Japonica Group] CBW45773 ORW1943Ba0077G13.1 [Oryza 0.80 2023 rufipogon] NP_001052976 Os04g0458200 [Oryza sativa 0.80 2024 2449 Japonica Group] &gt; gi|215715295|dbj|BAG95046.1| unnamed protein product [Oryza sativa Japonica Group] &gt; gi|218194967|gb|EEC77394.1| hypothetical protein OsI_16151 [Oryza sativa Indica Group] &gt; gi|255675525|dbj|BAF14890.2| Os04g0458200 [Oryza sativa Japonica Group] CAH67071 OSIGBa0097P08.1 [Oryza sativa 0.79 2025 2450 Indica Group] 1613-1631 NP_001141472 hypothetical protein 1.00 2026 2451 LOC100273582 [Zea mays] &gt; gi|194704716|gb|ACF86442.1| unknown [Zea mays] ACR33998 unknown [Zea mays] 0.85 2027 2452 Predicted 408-428 NP_001131645 hypothetical protein 1.00 2028 2453 siRNA LOC100193004 [Zea mays] 60850 &gt; gi|194692138|gb|ACF80153.1| unknown [Zea mays] 481-501 ACG31592 hypothetical protein [Zea mays] 1.00 2029 2454 NP_001132267 hypothetical protein 1.00 2030 2455 LOC100193703 [Zea mays] &gt; gi|194693920|gb|ACF81044.1| unknown [Zea mays] Predicted 1611-1630 XP_002463038 hypothetical protein 1.00 2031 2456 siRNA SORBIDRAFT_02g036610 59961 [Sorghum bicolor] &gt; gi|241926415|gb|EER99559.1| hypothetical protein SORBIDRAFT_02g036610 [Sorghum bicolor] NP_001130320 hypothetical protein 0.96 2032 2457 LOC100191414 [Zea mays] &gt; gi|194688838|gb|ACF78503.1| unknown [Zea mays] BAJ88611 predicted protein [Hordeum 0.87 2033 2458 vulgare subsp. vulgare] NP_001060016 Os07g0566200 [Oryza sativa 0.89 2034 2459 Japonica Group] &gt; gi|75152457|sp|Q8H4S6.2|P2C64_ORYSJ RecName: Full = Probable protein phosphatase 2C 64; Short = OsPP2C64 &gt; gi|33146759|dbj|BAC79670.1| putative protein phosphatase 2C [Oryza sativa Japonica Group] &gt; gi|113611552|dbj|BAF21930.1| Os07g0566200 [Oryza sativa Japonica Group] &gt; gi|125600752|gb|EAZ40328.1| hypothetical protein OsJ_24776 [Oryza sativa Japonica Group] NP_001150759 LOC100284392 [Zea mays] 0.92 2035 2460 &gt; gi|195641568|gb|ACG40252.1| catalytic/protein phosphatase type 2C [Zea mays] ACR38208 unknown [Zea mays] 0.92 2036 2461 EAZ04367 hypothetical protein OsI_26509 0.88 2037 [Oryza sativa Indica Group] ACN30598 unknown [Zea mays] 0.87 2038 2462 ACF84784 unknown [Zea mays] 0.80 2039 2463 802-821 XP_002462759 hypothetical protein 1.00 2040 2464 SORBIDRAFT_02g031500 [Sorghum bicolor] &gt; gi|241926136|gb|EER99280.1| hypothetical protein SORBIDRAFT_02g031500 [Sorghum bicolor] NP_001151289 RING-H2 finger protein ATL2B 0.80 2041 2465 [Zea mays] &gt; gi|195645562|gb|ACG42249.1| RING-H2 finger protein ATL2B [Zea mays] &gt; gi|223946553|gb|ACN27360.1| unknown [Zea mays] 540-559 XP_002438439 hypothetical protein 1.00 2042 2466 SORBIDRAFT_10g019640 [Sorghum bicolor] &gt; gi|241916662|gb|EER89806.1| hypothetical protein SORBIDRAFT_10g019640 [Sorghum bicolor] ACN33733 unknown [Zea mays] 0.89 2043 2467 NP_001150669 amino acid permease [Zea mays] 0.89 2044 2468 &gt; gi|195640964|gb|ACG39950.1| amino acid permease [Zea mays] XP_002453115 hypothetical protein 0.82 2045 2469 SORBIDRAFT_04g000290 [Sorghum bicolor] &gt; gi|241932946|gb|EES06091.1| hypothetical protein SORBIDRAFT_04g000290 [Sorghum bicolor] NP_001136459 hypothetical protein 0.80 2046 2470 LOC100216569 [Zea mays] &gt; gi|194695786|gb|ACF81977.1| unknown [Zea mays] NP_001045585 Os02g0101000 [Oryza sativa 0.78 2047 2471 Japonica Group] &gt; gi|41053220|dbj|BAD08181.1| putative amino acid transport protein [Oryza sativa Japonica Group] &gt; gi|113535116|dbj|BAF07499.1| Os02g0101000 [Oryza sativa Japonica Group] &gt; gi|215704334|dbj|BAG93768.1| unnamed protein product [Oryza sativa Japonica Group] &gt; gi|218189857|gb|EEC72284.1| hypothetical protein OsI_05452 [Oryza sativa Indica Group] &gt; gi|222621988|gb|EEE56120.1| hypothetical protein OsJ_04987 [Oryza sativa Japonica Group] BAJ96557 predicted protein [Hordeum 0.73 2048 2472 vulgare subsp. vulgare] &gt; gi|326523625|dbj|BAJ92983.1| predicted protein [Hordeum vulgare subsp. vulgare] XP_002446139 hypothetical protein 0.72 2049 2473 SORBIDRAFT_06g002240 [Sorghum bicolor] &gt; gi|241937322|gb|EES10467.1| hypothetical protein SORBIDRAFT_06g002240 [Sorghum bicolor] NP_001147785 amino acid permease [Zea mays] 0.71 2050 2474 &gt; gi|195613758|gb|ACG28709.1| amino acid permease [Zea mays] 987-1006 NP_001132785 hypothetical protein 1.00 2051 2475 LOC100194274 [Zea mays] &gt; gi|194695392|gb|ACF81780.1| unknown [Zea mays] NP_001150926 bHLH transcription factor GBOF- 0.96 2052 2476 1 [Zea mays] &gt; gi|195642998|gb|ACG40967.1| bHLH transcription factor GBOF-1 [Zea mays] XP_002462650 hypothetical protein 0.79 2053 2477 SORBIDRAFT_02g029530 [Sorghum bicolor] &gt; gi|241926027|gb|EER99171.1| hypothetical protein SORBIDRAFT_02g029530 [Sorghum bicolor] NP_001149110 LOC100282732 [Zea mays] 0.76 2054 2478 &gt; gi|195624818|gb|ACG34239.1| bHLH transcription factor GBOF-1 [Zea mays] ACR35934 unknown [Zea mays] 0.75 2055 2479 235-254 NP_001065691 Os11g0136600 [Oryza sativa 1.00 2480 Japonica Group] &gt; gi|33340240|gb|AAQ14593.1| AF319480_1 calcium-dependent calmodulin-independent protein kinase [Oryza sativa] &gt; gi|33340242|gb|AAQ14594.1| AF319481_1 calcium-dependent calmodulin-independent protein kinase [Oryza sativa] &gt; gi|77548548|gb|ABA91345.1| Calcium-dependent protein kinase, isoform 2, putative, expressed [Oryza sativa Japonica Group] &gt; gi|113644395|dbj|BAF27536.1| Os11g0136600 [Oryza sativa Japonica Group] Predicted 452-472 XP_002465086 hypothetical protein 1.00 2056 2481 siRNA SORBIDRAFT_01g031850 56542 [Sorghum bicolor] &gt; gi|241918940|gb|EER92084.1| hypothetical protein SORBIDRAFT_01g031850 [Sorghum bicolor] NP_001145189 hypothetical protein 0.92 2057 2482 LOC100278439 [Zea mays] &gt; gi|195652469|gb|ACG45702.1| hypothetical protein [Zea mays] NP_001050454 Os03g0439700 [Oryza sativa 0.78 2058 2483 Japonica Group] &gt; gi|108709042|gb|ABF96837.1| expressed protein [Oryza sativa Japonica Group] &gt; gi|113548925|dbj|BAF12368.1| Os03g0439700 [Oryza sativa Japonica Group] &gt; gi|222625197|gb|EEE59329.1| hypothetical protein OsJ_11404 [Oryza sativa Japonica Group] AAR89010 expressed protein [Oryza sativa 0.77 2059 Japonica Group] BAJ96331 predicted protein [Hordeum 0.77 2060 2484 vulgare subsp. vulgare] Predicted 585-608 NP_001183654 hypothetical protein 1.00 2061 2485 siRNA LOC100502248 [Zea mays] 56353 &gt; gi|238013682|gb|ACR37876.1| unknown [Zea mays] XP_002464467 hypothetical protein 0.94 2062 2486 SORBIDRAFT_01g018940 [Sorghum bicolor] &gt; gi|241918321|gb|EER91465.1| hypothetical protein SORBIDRAFT_01g018940 [Sorghum bicolor] AAL59033 hypothetical protein [Oryza sativa 0.78 2063 Japonica Group] &gt; gi|31432747|gb|AAP54340.1| expressed protein [Oryza sativa Japonica Group] BAJ89715 predicted protein [Hordeum 0.77 2064 2487 vulgare subsp. vulgare] &gt; gi|326505214|dbj|BAK02994.1| predicted protein [Hordeum vulgare subsp. vulgare] BAK03788 predicted protein [Hordeum 0.75 2065 2488 vulgare subsp. vulgare] NP_001064892 Os10g0484900 [Oryza sativa 0.76 2066 2489 Japonica Group] &gt; gi|255679504|dbj|BAF26806.2| Os10g0484900 [Oryza sativa Japonica Group] 23-46 XP_002463222 hypothetical protein 1.00 2067 2490 SORBIDRAFT_02g040060 [Sorghum bicolor] &gt; gi|241926599|gb|EER99743.1| hypothetical protein SORBIDRAFT_02g040060 [Sorghum bicolor] NP_001105697 thiazole biosynthetic enzyme 1-2, 0.93 2068 2491 chloroplastic precursor [Zea mays] &gt; gi|2501190|sp|Q41739.1|THI42_MAIZE RecName: Full = Thiazole biosynthetic enzyme 1-2, chloroplastic; Flags: Precursor &gt; gi|596080|gb|AAA96739.1| thiamine biosynthetic enzyme [Zea mays] ACF87708 unknown [Zea mays] 0.93 2069 2492 &gt; gi|224033955|gb|ACN36053.1| unknown [Zea mays] NP_001105696 thiazole biosynthetic enzyme 1-1, 0.92 2070 2493 chloroplastic precursor [Zea mays] &gt; gi|2501189|sp|Q41738.1|THI41_MAIZE RecName: Full = Thiazole biosynthetic enzyme 1-1, chloroplastic; Flags: Precursor &gt; gi|596078|gb|AAA96738.1| thiamine biosynthetic enzyme [Zea mays] &gt; gi|194704634|gb|ACF86401.1| unknown [Zea mays] NP_001059841 Os07g0529600 [Oryza sativa 0.87 2071 2494 Japonica Group] &gt; gi|32352138|dbj|BAC78562.1| thiamine biosynthetic enzyme [Oryza sativa Japonica Group] &gt; gi|113611377|dbj|BAF21755.1| Os07g0529600 [Oryza sativa Japonica Group] &gt; gi|215712225|dbj|BAG94352.1| unnamed protein product [Oryza sativa Japonica Group] BAC45141 putative thiamine biosynthesis 0.87 2072 2495 protein [Oryza sativa Japonica Group] &gt; gi|125600511|gb|EAZ40087.1| hypothetical protein OsJ_24530 [Oryza sativa Japonica Group] EAZ04139 hypothetical protein OsI_26282 0.87 2073 [Oryza sativa Indica Group] XP_002458014 hypothetical protein 0.91 2074 2496 SORBIDRAFT_03g025520 [Sorghum bicolor] &gt; gi|241929989|gb|EES03134.1| hypothetical protein SORBIDRAFT_03g025520 [Sorghum bicolor] ACF85034 unknown [Zea mays] 0.84 2075 2497 AAZ93636 pathogen-induced defense- 0.84 2076 2498 responsive protein 8 [Oryza sativa Indica Group] 71-94 ACG31888 ubiquitin-conjugating enzyme E2 1.00 2077 2499 W [Zea mays] NP_001050906 Os03g0681400 [Oryza sativa 0.71 2078 2500 Japonica Group] &gt; gi|57164486|gb|AAK00965.2| AC079736_5 putative ubiquitin- conjugating enzyme E2 [Oryza sativa Japonica Group] &gt; gi|108710422|gb|ABF98217.1| Ubiquitin-conjugating enzyme family protein, expressed [Oryza sativa Japonica Group] &gt; gi|113549377|dbj|BAF12820.1| Os03g0681400 [Oryza sativa Japonica Group] &gt; gi|215737341|dbj|BAG96270.1| unnamed protein product [Oryza sativa Japonica Group] &gt; gi|222625566|gb|EEE59698.1| hypothetical protein OsJ_12122 [Oryza sativa Japonica Group] XP_002464080 hypothetical protein 0.72 2079 2501 SORBIDRAFT_01g011940 [Sorghum bicolor] &gt; gi|241917934|gb|EER91078.1| hypothetical protein SORBIDRAFT_01g011940 [Sorghum bicolor] XP_002443656 hypothetical protein 0.71 2080 2502 SORBIDRAFT_08g022990 [Sorghum bicolor] &gt; gi|241944349|gb|EES17494.1| hypothetical protein SORBIDRAFT_08g022990 [Sorghum bicolor] NP_001146940 ubiquitin-conjugating enzyme E2 0.70 2081 2503 W [Zea mays] &gt; gi|195605468|gb|ACG24564.1| ubiquitin-conjugating enzyme E2 W [Zea mays] Predicted 614-634 NP_001047323 Os02g0596900 [Oryza sativa 1.00 2082 2504 siRNA Japonica Group] 59965 &gt; gi|75291091|sp|Q6K908.1|ARP3_ORYSJ RecName: Full = Actin-related protein 3 &gt; gi|190356069|sp|A2X6S3.2|ARP3_ORYSI RecName: Full = Actin-related protein 3 &gt; gi|47847830|dbj|BAD21625.1| putative arp3 [Oryza sativa Japonica Group] &gt; gi|113536854|dbj|BAF09237.1| Os02g0596900 [Oryza sativa Japonica Group] &gt; gi|218191098|gb|EEC73525.1| hypothetical protein OsI_07915 [Oryza sativa Indica Group] &gt; gi|222623169|gb|EEE57301.1| hypothetical protein OsJ_07378 [Oryza sativa Japonica Group] ACF87749 unknown [Zea mays] 0.92 2083 2505 &gt; gi|223972725|gb|ACN30550.1| unknown [Zea mays] XP_002285370 PREDICTED: hypothetical 0.86 2084 2506 protein [Vitis vinifera] NP_001147580 LOC100281189 [Zea mays] 0.92 2085 2507 &gt; gi|195612296|gb|ACG27978.1| actin-like protein 3 [Zea mays] NP_001170315 hypothetical protein 0.90 2086 2508 LOC100384280 [Zea mays] &gt; gi|224034999|gb|ACN36575.1| unknown [Zea mays] XP_002315648 predicted protein [Populus 0.84 2087 2509 trichocarpa] &gt; gi|222864688|gb|EEF01819.1| predicted protein [Populus trichocarpa] XP_002515041 protein binding protein, putative 0.85 2088 2510 [Ricinus communis] &gt; gi|223546092|gb|EEF47595.1| protein binding protein, putative [Ricinus communis] XP_002312654 predicted protein [Populus 0.84 2089 2511 trichocarpa] &gt; gi|222852474|gb|EEE90021.1| predicted protein [Populus trichocarpa] CAM97883 actin-related protein 3 [Nicotiana 0.83 2090 2512 tabacum] NP_172777 actin-related protein 3 0.81 2091 2513 [Arabidopsis thaliana] &gt; gi|75313139|sp|Q9SAF1.1|ARP3_ARATH RecName: Full = Actin-related protein 3; AltName: Full = Protein DISTORTED TRICHOMES 1 &gt; gi|4850401|gb|AAD31071.1| AC007357_20 Strong similarity to gb|U29610 Actin-like protein (Arp3) from Acanthamoeba castellanii and is a member of the PF|00022 Actin family [Arabidopsis thaliana] &gt; gi|21427461|gb|AAM53243.1| AF507911_1 actin-related protein 3 [Arabidopsis thaliana] &gt; gi|20260500|gb|AAM13148.1| similar to actin-like protein [Arabidopsis thaliana] &gt; gi|21489929|tpg|DAA00033.1| TPA_exp: actin-related protein 3; AtARP3 [Arabidopsis thaliana] &gt; gi|30387525|gb|AAP31928.1| At1g13180 [Arabidopsis thaliana] &gt; gi|332190859|gb|AEE28980.1| actin-related protein 3 [Arabidopsis thaliana] Predicted 632-651 ACR34392 unknown [Zea mays] 1.00 2092 2514 siRNA 58872 315-334 NP_001004443 beta-hexosaminidase subunit 1.00 2093 2515 alpha precursor [Rattus norvegicus] &gt; gi|85701350|sp|Q641X3.1|HEXA_RAT RecName: Full = Beta- hexosaminidase subunit alpha; AltName: Full = Beta-N- acetylhexosaminidase subunit alpha; Short = Hexosaminidase subunit A; AltName: Full = N- acetyl-beta-glucosaminidase subunit alpha; Flags: Precursor &gt; gi|51980341|gb|AAH82097.1| Hexosaminidase A [Rattus norvegicus] &gt; gi|149041858|gb|EDL95699.1| hexosaminidase A, isoform CRA_a [Rattus norvegicus] NP_034551 beta-hexosaminidase subunit 0.94 2094 2516 alpha precursor [Mus musculus] &gt; gi|497174|gb|AAA18775.1| beta-hexosaminidase [Mus musculus] &gt; gi|497196|gb|AAA18777.1| beta-hexosaminidase alpha- subunit [Mus musculus] &gt; gi|14789650|gb|AAH10755.1| Hexosaminidase A [Mus musculus] &gt; gi|26344774|dbj|BAC36036.1| unnamed protein product [Mus musculus] &gt; gi|26344800|dbj|BAC36049.1| unnamed protein product [Mus musculus] &gt; gi|74184438|dbj|BAE25744.1| unnamed protein product [Mus musculus] &gt; gi|74204649|dbj|BAE35394.1| unnamed protein product [Mus musculus] &gt; gi|148694024|gb|EDL25971.1| hexosaminidase A [Mus musculus] BAE35636 unnamed protein product [Mus 0.94 2095 2517 musculus] BAE29566 unnamed protein product [Mus 0.94 2096 2518 musculus] BAE30831 unnamed protein product [Mus 0.94 2097 2519 musculus] BAE35457 unnamed protein product [Mus 0.94 2098 2520 musculus] P29416 RecName: Full = Beta- 0.94 2099 hexosaminidase subunit alpha; AltName: Full = Beta-N- acetylhexosaminidase subunit alpha; Short = Hexosaminidase subunit A; AltName: Full = N- acetyl-beta-glucosaminidase subunit alpha; Flags: Precursor &gt; gi|51265|emb|CAA45615.1| beta-N-acetylhexosaminidase [Mus musculus] &gt; gi|577688|gb|AAC53246.1| beta-N-acetylhexosaminidase, alpha-subunit [Mus musculus] BAC38018 unnamed protein product [Mus 0.94 2100 2521 musculus] BAE39189 unnamed protein product [Mus 0.94 2101 2522 musculus] BAE40146 unnamed protein product [Mus 0.94 2102 2523 musculus] 535-554 XP-002526446 heat shock protein, putative 1.00 2103 2524 [Ricinus communis] &gt; gi|223534226|gb|EEF35941.1| heat shock protein, putative [Ricinus communis] XP_002328713 predicted protein [Populus 0.92 2104 2525 trichocarpa] &gt; gi|222839011|gb|EEE77362.1| predicted protein [Populus trichocarpa] XP_002331133 predicted protein [Populus 0.92 2105 2526 trichocarpa] &gt; gi|222872861|gb|EEF09992.1| predicted protein [Populus trichocarpa] XP_002279101 PREDICTED: hypothetical 0.93 2106 2527 protein [Vitis vinifera] ABZ04081 chloroplast heat shock protein 70- 0.90 2107 2528 2 [Ipomoea nil] ABM92419 chloroplast HSP70 [Cucumis 0.90 2108 2529 sativus] ABE79560 Chaperone DnaK [Medicago 0.90 2109 2530 truncatula] CAA52149 heat shock protein 70 [Cucumis 0.89 2110 2531 sativus] Q02028 RecName: Full = Stromal 70 kDa 0.90 2111 heat shock-related protein, chloroplastic; Flags: Precursor &gt; gi|169023|gb|AAA33637.1| 70 kDa heat shock protein [Pisum sativum] &gt; gi|871515|emb|CAA49147.1| Psst70 (stress 70 protein) [Pisum sativum] CAN81065 hypothetical protein 0.91 2112 2532 VITISV_000728 [Vitis vinifera] Predicted 1842-1866 YP_024387 ribulose-1,5-bisphosphate 1.00 2113 2533 siRNA carboxylase/oxygenase large 54631 subunit [Saccharum hybrid cultivar SP-80-3280] &gt; gi|50812536|ref|YP_054639.1| ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit [Saccharum officinarum] &gt; gi|118614500|ref|YP_899415.1| ribulose 1,5-bisphosphate carboxylase/oxygenase large subunit [Sorghum bicolor] &gt; gi|75290174|sp|Q6ENV5.1|RBL_SACOF RecName: Full = Ribulose bisphosphate carboxylase large chain; Short = RuBisCO large subunit; Flags: Precursor &gt; gi|75291223|sp|Q6L391.1|RBL_SACHY RecName: Full = Ribulose bisphosphate carboxylase large chain; Short = RuBisCO large subunit; Flags: Precursor &gt; gi|125987520|sp|A1E9T2.1|RBL_SORBI RecName: Full = Ribulose bisphosphate carboxylase large chain; Short = RuBisCO large subunit; Flags: Precursor &gt; gi|48478681|gb|AAT44701.1| RuBisCO large chain [Saccharum hybrid cultivar SP80-3280] &gt; gi|49659520|dbj|BAD27301.1| RuBisCO large subunit [Saccharum hybrid cultivar NCo 310] &gt; gi|118201134|gb|ABK79504.1| ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit [Sorghum bicolor] NP_043033 ribulose-1,5-bisphosphate 0.99 2114 2534 carboxylase/oxygenase large subunit [Zea mays] &gt; gi|1172861|sp|P00874.2|RBL_MAIZE RecName: Full = Ribulose bisphosphate carboxylase large chain; Short = RuBisCO large subunit; Flags: Precursor &gt; gi|18036|emb|CAA78027.1| Ribulose bisphosphate carboxylase [Zea mays] &gt; gi|902230|emb|CAA60294.1| ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit [Zea mays] ABP01447 ribulose-1,5-bisphosphate 0.99 2115 2535 carboxylase/oxygenase large subunit [Sorghastrum nutans] YP_003208195 ribulose-1,5-bisphosphate 0.98 2116 2536 carboxylase/oxygenase large subunit [Coix lacryma-jobi] &gt; gi|209361365|gb|ACI43280.1| ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit [Coix lacryma-jobi] ABP01443 ribulose-1,5-bisphosphate 0.98 2117 2537 carboxylase/oxygenase large subunit [Cymbopogon citratus] ABP01445 ribulose-1,5-bisphosphate 0.98 2118 2538 carboxylase/oxygenase large subunit [Pogonatherum sp. Hodkinson 21] ABP01442 ribulose-1,5-bisphosphate 0.97 2119 2539 carboxylase/oxygenase large subunit [Coix lacryma-jobi] ABP01448 ribulose-1,5-bisphosphate 0.96 2120 2540 carboxylase/oxygenase large subunit [Sorghum halepense] CAG34130 ribulose-1,5-bisphosphate 0.96 2121 2541 carboxylase/oxygenase large subunit [Arthraxon sp. Hodkinson 111] AAA31678 ribulose 1,5-bisphosphate 0.97 2122 2542 carboxylase/oxygenase [Cenchrus setiger] &gt; gi|294104|gb|AAA32020.1| ribulosebiphosphate carboxylase, large subunit [Pennisetum glaucum] 884-908 ABP01449 ribulose-1,5-bisphosphate 1.00 2123 2543 carboxylase/oxygenase large subunit [Trachypogon spicatus] ABP01447 ribulose-1,5-bisphosphate 1.00 2124 2544 carboxylase/oxygenase large subunit [Sorghastrum nutans] NP_043033 ribulose-1,5-bisphosphate 0.99 2125 2545 carboxylase/oxygenase large subunit [Zea mays] &gt; gi|1172861|sp|P00874.2|RBL_MAIZE RecName: Full = Ribulose bisphosphate carboxylase large chain; Short = RuBisCO large subunit; Flags: Precursor &gt; gi|18036|emb|CAA78027.1| Ribulose bisphosphate carboxylase [Zea mays] &gt; gi|902230|emb|CAA60294.1| ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit [Zea mays] YP_024387 ribulose-1,5-bisphosphate 1.00 2126 2546 carboxylase/oxygenase large subunit [Saccharum hybrid cultivar SP-80-3280] &gt; gi|50812536|ref|YP_054639.1| ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit [Saccharum officinarum] &gt; gi|118614500|ref|YP_899415.1| ribulose 1,5-bisphosphate carboxylase/oxygenase large subunit [Sorghum bicolor] &gt; gi|75290174|sp|Q6ENV5.1|RBL_SACOF RecName: Full = Ribulose bisphosphate carboxylase large chain; Short = RuBisCO large subunit; Flags: Precursor &gt; gi|75291223|sp|Q6L391.1|RBL_SACHY RecName: Full = Ribulose bisphosphate carboxylase large chain; Short = RuBisCO large subunit; Flags: Precursor &gt; gi|125987520|sp|A1E9T2.1|RBL_SORBI RecName: Full = Ribulose bisphosphate carboxylase large chain; Short = RuBisCO large subunit; Flags: Precursor &gt; gi|48478681|gb|AAT44701.1| RuBisCO large chain [Saccharum hybrid cultivar SP80-3280] &gt; gi|49659520|dbj|BAD27301.1| RuBisCO large subunit [Saccharum hybrid cultivar NCo 310] &gt; gi|118201134|gb|ABK79504.1| ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit [Sorghum bicolor] ABP01443 ribulose-1,5-bisphosphate 1.00 2127 2547 carboxylase/oxygenase large subunit [Cymbopogon citratus] ABP01445 ribulose-1,5-bisphosphate 0.99 2128 2548 carboxylase/oxygenase large subunit [Pogonatherum sp. Hodkinson 21] ABP01442 ribulose-1,5-bisphosphate 0.98 2129 2549 carboxylase/oxygenase large subunit [Coix lacryma-jobi] YP_003208195 ribulose-1,5-bisphosphate 0.98 2130 2550 carboxylase/oxygenase large subunit [Coix lacryma-jobi] &gt; gi|209361365|gb|ACI43280.1| ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit [Coix lacryma-jobi] ABP01441 ribulose-1,5-bisphosphate 0.98 2131 2551 carboxylase/oxygenase large subunit [Bothriochloa ischaemum] CAG34130 ribulose-1,5-bisphosphate 0.96 2132 2552 carboxylase/oxygenase large subunit [Arthraxon sp. Hodkinson 111] Predicted 69-92 XP_002458082 hypothetical protein 1.00 2133 2553 siRNA SORBIDRAFT_03g026670 59846 [Sorghum bicolor] &gt; gi|241930057|gb|EES03202.1| hypothetical protein SORBIDRAFT_03g026670 [Sorghum bicolor] EEC70994 hypothetical protein OsI_02661 0.83 2134 [Oryza sativa Indica Group] NP_001152397 regulator of telomere elongation 0.78 2135 2554 helicase 1 [Zea mays] &gt; gi|195655863|gb|ACG47399.1| regulator of telomere elongation helicase 1 [Zea mays] NP_001043456 Os01g0592900 [Oryza sativa 0.70 2136 2555 Japonica Group] &gt; gi|53791584|dbj|BAD52706.1| DEAH helicase isoform 5-like [Oryza sativa Japonica Group] &gt; gi|255673416|dbj|BAF05370.2| Os01g0592900 [Oryza sativa Japonica Group]  85-108 ACR34511 unknown [Zea mays] 1.00 2137 2556 NP_001105187 histone acetyltransferase1 [Zea 0.99 2138 2557 mays] &gt; gi|20977602|gb|AAM28228.1| histone acetyl transferase [Zea mays] &gt; gi|223975399|gb|ACN31887.1| unknown [Zea mays] Q8LPU4 RecName: Full = Histone 0.99 2139 acetyltransferase type B catalytic subunit; AltName: Full = Histone acetyltransferase HAT B; AltName: Full = Histone acetyltransferase HAT-B-p50 &gt; gi|6288802|gb|AAF06742.1| AF171927_1 histone acetyltransferase HAT-B-p50 [Zea mays] &gt; gi|5579441|gb|AAC03423.2| histone acetyltransferase HAT B [Zea mays] XP_002460092 hypothetical protein 0.89 2140 2558 SORBIDRAFT_02g022640 [Sorghum bicolor] &gt; gi|241923469|gb|EER96613.1| hypothetical protein SORBIDRAFT_02g022640 [Sorghum bicolor] NP_001062946 Os09g0347800 [Oryza sativa 0.77 2141 2559 Japonica Group] &gt; gi|75121858|sp|Q6ES10.1|HAT1_ORYSJ RecName: Full = Probable acetyltransferase type B catalytic subunit; AltName: Full = HAT B &gt; gi|50252375|dbj|BAD28482.1| putative histone acetyltransferase HAT B [Oryza sativa Japonica Group] &gt; gi|50252405|dbj|BAD28560.1| putative histone acetyltransferase HAT B [Oryza sativa Japonica Group] &gt; gi|113631179|dbj|BAF24860.1| Os09g0347800 [Oryza sativa Japonica Group] &gt; gi|215678892|dbj|BAG95329.1| unnamed protein product [Oryza sativa Japonica Group] EEC84410 hypothetical protein OsI_30998 0.77 2142 [Oryza sativa Indica Group] EEE69531 hypothetical protein OsJ_29003 0.77 2143 [Oryza sativa Japonica Group] BAK05443 predicted protein [Hordeum 0.75 2144 2560 vulgare subsp. vulgare]  85-108 XP_002460092 hypothetical protein 1.00 2145 2561 SORBIDRAFT_02g022640 [Sorghum bicolor] &gt; gi|241923469|gb|EER96613.1| hypothetical protein SORBIDRAFT_02g022640 [Sorghum bicolor] NP_001105187 histone acetyltransferase1 [Zea 0.90 2146 2562 mays] &gt; gi|20977602|gb|AAM28228.1| histone acetyl transferase [Zea mays] &gt; gi|223975399|gb|ACN31887.1| unknown [Zea mays] Q8LPU4 RecName: Full = Histone 0.90 2147 acetyltransferase type B catalytic subunit; AltName: Full = Histone acetyltransferase HAT B; AltName: Full = Histone acetyltransferase HAT-B-p50 &gt; gi|6288802|gb|AAF06742.1| AF171927_1 histone acetyltransferase HAT-B-p50 [Zea mays] &gt; gi|5579441|gb|AAC03423.2| histone acetyltransferase HAT B [Zea mays] NP_001062946 Os09g0347800 [Oryza sativa 0.81 2148 2563 Japonica Group] &gt; gi|75121858|sp|Q6ES10.1|HAT1_ORYSJ RecName: Full = Probable acetyltransferase type B catalytic subunit; AltName: Full = HAT B &gt; gi|50252375|dbj|BAD28482.1| putative histone acetyltransferase HAT B [Oryza sativa Japonica Group] &gt; gi|50252405|dbj|BAD28560.1| putative histone acetyltransferase HAT B [Oryza sativa Japonica Group] &gt; gi|113631179|dbj|BAF24860.1| Os09g0347800 [Oryza sativa Japonica Group] &gt; gi|215678892|dbj|BAG95329.1| unnamed protein product [Oryza sativa Japonica Group] EEC84410 hypothetical protein OsI_30998 0.81 2149 [Oryza sativa Indica Group] EEE69531 hypothetical protein OsJ_29003 0.81 2150 [Oryza sativa Japonica Group] BAK05443 predicted protein [Hordeum 0.78 2151 2564 vulgare subsp. vulgare] ACR34511 unknown [Zea mays] 0.79 2152 2565 Predicted 293-311 XP_002460816 hypothetical protein 1.00 2153 2566 siRNA SORBIDRAFT_02g035420 60081 [Sorghum bicolor] &gt; gi|241924193|gb|EER97337.1| hypothetical protein SORBIDRAFT_02g035420 [Sorghum bicolor] NP_001147287 receptor-like serine-threonine 0.87 2154 2567 protein kinase [Zea mays] &gt; gi|195609532|gb|ACG26596.1| receptor-like serine-threonine protein kinase [Zea mays] BAK06110 predicted protein [Hordeum 0.73 2155 2568 vulgare subsp. vulgare] 564-582 ACN27203 unknown [Zea mays] 1.00 2156 2569 NP_001150572 RING-H2 finger protein ATL1R 1.00 2157 2570 [Zea mays] &gt; gi|195640284|gb|ACG39610.1| RING-H2 finger protein ATL1R [Zea mays] XP_002448400 hypothetical protein 0.80 2158 2571 SORBIDRAFT_06g026580 [Sorghum bicolor] &gt; gi|241939583|gb|EES12728.1| hypothetical protein SORBIDRAFT_06g026580 [Sorghum bicolor] 374-392 NP_001151643 DNA binding protein [Zea mays] 1.00 2159 2572 &gt; gi|195648318|gb|ACG43627.1| DNA binding protein [Zea mays] XP_002468594 hypothetical protein 0.83 2160 2573 SORBIDRAFT_01g048710 [Sorghum bicolor] &gt; gi|241922448|gb|EER95592.1| hypothetical protein SORBIDRAFT_01g048710 [Sorghum bicolor] NP_001048832 Os03g0127500 [Oryza sativa 0.73 2161 2574 Japonica Group] &gt; gi|20330751|gb|AAM19114.1| AC104427_12 Putative bZIP transcription factor [Oryza sativa Japonica Group] &gt; gi|108705971|gb|ABF93766.1| bZIP family transcription factor, putative, expressed [Oryza sativa Japonica Group] &gt; gi|113547303|dbj|BAF10746.1| Os03g0127500 [Oryza sativa Japonica Group] EAZ25438 hypothetical protein OsJ_09254 0.73 2162 [Oryza sativa Japonica Group] EAY88362 hypothetical protein OsI_09817 0.73 2163 [Oryza sativa Indica Group] 365-383 XP_002446810 hypothetical protein 1.00 2164 2575 SORBIDRAFT_06g023020 [Sorghum bicolor] &gt; gi|241937993|gb|EES11138.1| hypothetical protein SORBIDRAFT_06g023020 [Sorghum bicolor] NP_001185810 yellow stripe-like transporter 11 0.90 2165 2576 [Zea mays] &gt; gi|308210134|gb|ADO20998.1| yellow stripe-like transporter 11 [Zea mays] CAH67887 OSIGBa0153E02- 0.79 2166 OSIGBa0093I20.16 [Oryza sativa Indica Group] &gt; gi|125549079|gb|EAY94901.1| hypothetical protein OsI_16701 [Oryza sativa Indica Group] Q7X660 RecName: Full = Probable metal- 0.79 2167 nicotianamine transporter YSL11; AltName: Full = Protein YELLOW STRIPE LIKE 11; Short = OsYSL11 &gt; gi|32487645|emb|CAE05635.1| OSJNBa0038O10.1 [Oryza sativa Japonica Group] &gt; gi|57834127|emb|CAI44638.1| OSJNBb0065J09.18 [Oryza sativa Japonica Group] &gt; gi|125591037|gb|EAZ31387.1| hypothetical protein OsJ_15515 [Oryza sativa Japonica Group] BAE91891 hypothetical protein [Oryza sativa 0.79 2168 2577 Japonica Group] Q5JQD7 RecName: Full = Probable metal- 0.74 2169 nicotianamine transporter YSL12; AltName: Full = Protein YELLOW STRIPE LIKE 12; Short = OsYSL12 &gt; gi|116310949|emb|CAH67886.1| OSIGBa0153E02- OSIGBa0093I20.15 [Oryza sativa Indica Group] NP_001185468 yellow stripe-like transporter 12 0.73 2170 2578 [Zea mays] &gt; gi|308210136|gb|ADO20999.1| yellow stripe-like transporter 12 [Zea mays] XP_002452492 hypothetical protein 0.71 2171 2579 SORBIDRAFT_04g026840 [Sorghum bicolor] &gt; gi|241932323|gb|EES05468.1| hypothetical protein SORBIDRAFT_04g026840 [Sorghum bicolor] BAE91892 hypothetical protein [Oryza sativa 0.73 2172 2580 Japonica Group] XP_002446809 hypothetical protein 0.72 2173 2581 SORBIDRAFT_06g023010 [Sorghum bicolor] &gt; gi|241937992|gb|EES11137.1| hypothetical protein SORBIDRAFT_06g023010 [Sorghum bicolor] 529-547 XP_002464238 hypothetical protein 1.00 2174 2582 SORBIDRAFT_01g014760 [Sorghum bicolor] &gt; gi|241918092|gb|EER91236.1| hypothetical protein SORBIDRAFT_01g014760 [Sorghum bicolor] NP_001146578 hypothetical protein 0.92 2175 2583 LOC100280174 [Zea mays] &gt; gi|219887889|gb|ACL54319.1| unknown [Zea mays] ACN27618 unknown [Zea mays] 0.91 2176 2584 EAY90980 hypothetical protein OsI_12589 0.83 2177 [Oryza sativa Indica Group] NP_001050649 Os03g0609800 [Oryza sativa 0.83 2178 2585 Japonica Group] &gt; gi|37700299|gb|AAR00589.1| putative NPH3 family protein [Oryza sativa Japonica Group] &gt; gi|40539093|gb|AAR87349.1| transposon protein, putative, mutator sub-class [Oryza sativa Japonica Group] &gt; gi|62733727|gb|AAX95837.1| transposon protein, putative, mutator sub-class [Oryza sativa Japonica Group] &gt; gi|108709776|gb|ABF97571.1| transposon protein, putative, Mutator sub-class, expressed [Oryza sativa Japonica Group] &gt; gi|108709777|gb|ABF97572.1| transposon protein, putative, Mutator sub-class, expressed [Oryza sativa Japonica Group] &gt; gi|113549120|dbj|BAF12563.1| Os03g0609800 [Oryza sativa Japonica Group] &gt; gi|125587097|gb|EAZ27761.1| hypothetical protein OsJ_11706 [Oryza sativa Japonica Group] &gt; gi|215686957|dbj|BAG89762.1| unnamed protein product [Oryza sativa Japonica Group] EAZ21022 hypothetical protein OsJ_36671 0.73 2179 [Oryza sativa Japonica Group] NP_001067147 Os12g0583500 [Oryza sativa 0.73 2180 2586 Japonica Group] &gt; gi|77556906|gb|ABA99702.1| transposon protein, putative, Mutator sub-class, expressed [Oryza sativa Japonica Group] &gt; gi|113649654|dbj|BAF30166.1| Os12g0583500 [Oryza sativa Japonica Group] EAY83679 hypothetical protein OsI_38903 0.73 2181 [Oryza sativa Indica Group] BAJ87350 predicted protein [Hordeum 0.71 2182 2587 vulgare subsp. vulgare] 3078-3096 NP_001185468 yellow stripe-like transporter 12 1.00 2183 2588 [Zea mays] &gt; gi|308210136|gb|ADO20999.1| yellow stripe-like transporter 12 [Zea mays] XP_002446809 hypothetical protein 0.93 2184 2589 SORBIDRAFT_06g023010 [Sorghum bicolor] &gt; gi|241937992|gb|EES11137.1| hypothetical protein SORBIDRAFT_06g023010 [Sorghum bicolor] Q5JQD7 RecName: Full = Probable metal- 0.88 2185 nicotianamine transporter YSL12; AltName: Full = Protein YELLOW STRIPE LIKE 12; Short = OsYSL12 &gt; gi|116310949|emb|CAH67886.1| OSIGBa0153E02- OSIGBa0093I20.15 [Oryza sativa Indica Group] BAE91892 hypothetical protein [Oryza sativa 0.87 2186 2590 Japonica Group] XP_002452492 hypothetical protein 0.82 2187 2591 SORBIDRAFT_04g026840 [Sorghum bicolor] &gt; gi|241932323|gb|EES05468.1| hypothetical protein SORBIDRAFT_04g026840 [Sorghum bicolor] BAE44205 hypothetical protein [Oryza sativa 0.81 2188 2592 Japonica Group] Q6H7J6 RecName: Full = Probable metal- 0.81 2189 nicotianamine transporter YSL14; AltName: Full = Protein YELLOW STRIPE LIKE 14; Short = OsYSL14 &gt; gi|49388177|dbj|BAD25303.1| oligopeptide transporter OPT- like [Oryza sativa Japonica Group] BAJ89062 predicted protein [Hordeum 0.80 2190 2593 vulgare subsp. vulgare] BAJ92335 predicted protein [Hordeum 0.80 2191 2594 vulgare subsp. vulgare] ACN34648 unknown [Zea mays] 0.81 2192 2595 &gt; gi|295441975|gb|ADG21035.1| oligopeptide transporter [Zea mays] &gt; gi|308210138|gb|ADO21000.1| yellow stripe-like transporter 14A [Zea mays] Predicted 1927-1945 XP_002450269 hypothetical protein 1.00 2193 2596 siRNA SORBIDRAFT_05g002900 59380 [Sorghum bicolor] &gt; gi|241936112|gb|EES09257.1| hypothetical protein SORBIDRAFT_05g002900 [Sorghum bicolor] NP_001130515 hypothetical protein 0.98 2194 2597 LOC100191614 [Zea mays] &gt; gi|194689354|gb|ACF78761.1| unknown [Zea mays] &gt; gi|195635013|gb|ACG36975.1| pyruvate kinase, cytosolic isozyme [Zea mays] &gt; gi|223947285|gb|ACN27726.1| unknown [Zea mays] &gt; gi|238009496|gb|ACR35783.1| unknown [Zea mays] NP_001149198 LOC100282820 [Zea mays] 0.97 2195 2598 &gt; gi|195625378|gb|ACG34519.1| pyruvate kinase, cytosolic isozyme [Zea mays] BAJ87403 predicted protein [Hordeum 0.95 2196 2599 vulgare subsp. vulgare] BAJ94667 predicted protein [Hordeum 0.95 2197 2600 vulgare subsp. vulgare] NP_001065749 Os11g0148500 [Oryza sativa 0.95 2198 2601 Japonica Group] &gt; gi|77548686|gb|ABA91483.1| pyruvate kinase family protein, expressed [Oryza sativa Japonica Group] &gt; gi|113644453|dbj|BAF27594.1| Os11g0148500 [Oryza sativa Japonica Group] &gt; gi|215692631|dbj|BAG88051.1| unnamed protein product [Oryza sativa Japonica Group] &gt; gi|218185248|gb|EEC67675.1| hypothetical protein OsI_35105 [Oryza sativa Indica Group] &gt; gi|222615524|gb|EEE51656.1| hypothetical protein OsJ_32969 [Oryza sativa Japonica Group] NP_001066148 Os12g0145700 [Oryza sativa 0.94 2199 2602 Japonica Group] &gt; gi|77553678|gb|ABA96474.1| pyruvate kinase family protein, expressed [Oryza sativa Japonica Group] &gt; gi|113648655|dbj|BAF29167.1| Os12g0145700 [Oryza sativa Japonica Group] &gt; gi|215701048|dbj|BAG92472.1| unnamed protein product [Oryza sativa Japonica Group] &gt; gi|218186432|gb|EEC68859.1| hypothetical protein OsI_37456 [Oryza sativa Indica Group] &gt; gi|222616632|gb|EEE52764.1| hypothetical protein OsJ_35209 [Oryza sativa Japonica Group] XP_002283911 PREDICTED: hypothetical 0.89 2200 2603 protein [Vitis vinifera] AAM22747 pyruvate kinase-like 0.88 2201 2604 [Deschampsia antarctica] ABE80121 Pyruvate kinase [Medicago 0.89 2202 truncatula] Predicted 43-66 NP_001052992 Os04g0460600 [Oryza sativa 1.00 2203 2605 siRNA Japonica Group] 55806 &gt; gi|38344965|emb|CAD40985.2| OSJNBa0072F16.10 [Oryza sativa Japonica Group] &gt; gi|108947442|gb|ABF47345.2| NAC domain protein [Oryza sativa Japonica Group] &gt; gi|113564563|dbj|BAF14906.1| Os04g0460600 [Oryza sativa Japonica Group] &gt; gi|116310448|emb|CAH67453.1| H0219H12.10 [Oryza sativa Indica Group] &gt; gi|215692665|dbj|BAG88085.1| unnamed protein product [Oryza sativa Japonica Group] &gt; gi|215737715|dbj|BAG96845.1| unnamed protein product [Oryza sativa Japonica Group] &gt; gi|218194979|gb|EEC77406.1| hypothetical protein OsI_16171 [Oryza sativa Indica Group] &gt; gi|222628996|gb|EEE61128.1| hypothetical protein OsJ_15058 [Oryza sativa Japonica Group] BAC53811 OsNAC2 protein [Oryza sativa] 0.97 2204 2606 XP_002446618 hypothetical protein 0.71 2205 2607 SORBIDRAFT_06g019010 [Sorghum bicolor] &gt; gi|241937801|gb|EES10946.1| hypothetical protein SORBIDRAFT_06g019010 [Sorghum bicolor] Predicted 2555-2578 NP_001141674 hypothetical protein 1.00 2206 2608 siRNA LOC100273800 [Zea mays] 57034 &gt; gi|194705506|gb|ACF86837.1| unknown [Zea mays] XP_002451554 hypothetical protein 0.74 2207 2609 SORBIDRAFT_04g003650 [Sorghum bicolor] &gt; gi|241931385|gb|EES04530.1| hypothetical protein SORBIDRAFT_04g003650 [Sorghum bicolor] 95-115 YP_874773 ribosomal protein L14 [Agrostis 1.00 2208 2610 stolonifera] &gt; gi|118430423|ref|YP_874689.1| ribosomal protein L14 [Hordeum vulgare subsp. vulgare] &gt; gi|159106900|ref|YP_001531318.1| ribosomal protein L14 [Lolium perenne] &gt; gi|125987532|sp|A1EA45.1|RK14_AGRST RecName: Full = 50S ribosomal protein L14, chloroplastic &gt; gi|218546804|sp|A1E9M7.1| RK14_HORVU RecName: Full = 50S ribosomal protein L14, chloroplastic &gt; gi|218546811|sp|A8Y9C3.1|RK14_LOLPR RecName: Full = 50S ribosomal protein L14, chloroplastic &gt; gi|118201077|gb|ABK79448.1| ribosomal protein L14 [Hordeum vulgare subsp. vulgare] &gt; gi|118201247|gb|ABK79616.1| ribosomal protein L14 [Agrostis stolonifera] &gt; gi|158934434|emb|CAO86012.1| ribosomal protein L14 [Lolium perenne] YP_002364534 ribosomal protein L14 [Festuca 0.99 2209 2611 arundinacea] &gt; gi|215882361|gb|ACJ70791.1| ribosomal protein L14 [Festuca arundinacea] NP_114294 ribosomal protein L14 [Triticum 0.99 2210 2612 aestivum] &gt; gi|45477200|sp|Q95H51.1|RK14_WHEAT RecName: Full = 50S ribosomal protein L14, chloroplastic &gt; gi|13928241|dbj|BAB47070.1| ribosomal protein L14 [Triticum aestivum] NP_039422 ribosomal protein L14 [Oryza 0.98 2211 2613 sativa Japonica Group] &gt; gi|50234008|ref|YP_052786.1| ribosomal protein L14 [Oryza nivara] &gt; gi|109156621|ref|YP_654240.1| ribosomal protein L14 [Oryza sativa Indica Group] &gt; gi|253729591|ref|YP_003029775.1| ribosomal protein L14 [Bambusa oldhamii] &gt; gi|255961418|ref|YP_003097611.1| ribosomal protein L14 [Dendrocalamus latiflorus] &gt; gi|297723921|ref|NP_001174324.1| Os05g0292700 [Oryza sativa Japonica Group] &gt; gi|68565732|sp|Q6END7.1|RK14_ORYNI RecName: Full = 50S ribosomal protein L14, chloroplastic &gt; gi|148839610|sp|P0C438.1|RK14_ORYSA RecName: Full = 50S ribosomal protein L14, chloroplastic &gt; gi|148839611|sp|P0C439.1|RK14_ORYSI RecName: Full = 50S ribosomal protein L14, chloroplastic &gt; gi|148839612|sp|P0C440.1|RK14_ORYSJ RecName: Full = 50S ribosomal protein L14, chloroplastic &gt; gi|12023|emb|CAA33932.1| ribosomal protein L14 [Oryza sativa Japonica Group] &gt; gi|20160833|dbj|BAB89773.1| Chloroplast ribosomal protein L14 [Oryza sativa Japonica Group] &gt; gi|40253571|dbj|BAD05517.1| ribosomal protein L14 [Oryza sativa Japonica Group] &gt; gi|42795513|gb|AAS46080.1| ribosomal protein L14 [Oryza sativa Indica Group] &gt; gi|49615032|dbj|BAD26815.1| ribosomal protein L14 [Oryza nivara] &gt; gi|246367101|gb|ACS94712.1| ribosomal protein L14 [Bambusa oldhamii] &gt; gi|255040295|gb|ACT99955.1| ribosomal protein L14 [Dendrocalamus latiflorus] &gt; gi|255676212|dbj|BAH93052.1| Os05g0292700 [Oryza sativa Japonica Group] &gt; gi|307133922|gb|ADN32927.1| ribosomal protein L14 [Phyllostachys nigra var. henonis] &gt; gi|309321653|gb|ADO65178.1| ribosomal protein L14 [Acidosasa purpurea] &gt; gi|309321737|gb|ADO65261.1| ribosomal protein L14 [Ferrocalamus rimosivaginus] &gt; gi|309321821|gb|ADO65344.1| ribosomal protein L14 [Indocalamus longiauritus] &gt; gi|309321904|gb|ADO65426.1| ribosomal protein L14 [Phyllostachys edulis] &gt; gi|309321989|gb|ADO65510.1| ribosomal protein L14 [Bambusa emeiensis] &gt; gi|226644|prf||1603356BU ribosomal protein L14 YP_00200522 ribosomal protein L14 0.98 2212 2614 [Brachypodium distachyon] &gt; gi|218546902|sp|B3TN86.1|RK14_BRADI RecName: Full = 50S ribosomal protein L14, chloroplastic &gt; gi|193075591|gb|ACF08674.1| ribosomal protein L14 [Brachypodium distachyon] ABJ52167 ribosomal protein L14 0.98 2213 2615 [Phyllostachys edulis] BAC10087 Chloroplast 50S ribosomal protein 0.98 2214 2616 L14 [Oryza sativa Japonica Group] &gt; gi|50510231|dbj|BAD31429.1| Chloroplast 50S ribosomal protein L14 [Oryza sativa Japonica Group] YP_003587704 ribosomal protein L14 0.95 2215 2617 [Anomochloa marantoidea] &gt; gi|251765288|gb|ACT15442.1| ribosomal protein L14 [Anomochloa marantoidea] BAD33446 putative ribosomal protein L14 0.96 2216 2618 [Oryza sativa Japonica Group] &gt; gi|50726203|dbj|BAD33722.1| putative ribosomal protein L14 [Oryza sativa Japonica Group] AAT85078 putative 50S ribosomal protein 0.94 2217 2619 L14 [Oryza sativa Japonica Group] &gt; gi|50878445|gb|AAT85219.1| putative 50S ribosomal protein L14 [Oryza sativa Japonica Group] Predicted 403-422 XP_002439358 hypothetical protein 1.00 2218 2620 siRNA SORBIDRAFT_09g005070 60387 [Sorghum bicolor] &gt; gi|241944643|gb|EES17788.1| hypothetical protein SORBIDRAFT_09g005070 [Sorghum bicolor] NP_001140641 hypothetical protein 0.88 2219 2621 LOC100272716 [Zea mays] &gt; gi|194700306|gb|ACF84237.1| unknown [Zea mays] ACG56678 tryptophan aminotransferase [Zea 0.88 2220 2622 mays] NP_001151869 alliin lyase 2 [Zea mays] 0.79 2221 2623 &gt; gi|195650459|gb|ACG44697.1| alliin lyase 2 precursor [Zea mays] 374-393 NP_001140641 hypothetical protein 1.00 2222 2624 LOC100272716 [Zea mays] &gt; gi|194700306|gb|ACF84237.1| unknown [Zea mays] ACG56678 tryptophan aminotransferase [Zea 1.00 2223 2625 mays] XP_002439358 hypothetical protein 0.89 2224 2626 SORBIDRAFT_09g005070 [Sorghum bicolor] &gt; gi|241944643|gb|EES17788.1| hypothetical protein SORBIDRAFT_09g005070 [Sorghum bicolor] NP_001151869 alliin lyase 2 [Zea mays] 0.77 2225 2627 &gt; gi|195650459|gb|ACG44697.1| alliin lyase 2 precursor [Zea mays] 155-174 XP_002446287 hypothetical protein 1.00 2226 2628 SORBIDRAFT_06g013470 [Sorghum bicolor] &gt; gi|241937470|gb|EES10615.1| hypothetical protein SORBIDRAFT_06g013470 [Sorghum bicolor] NP_001132422 hypothetical protein 0.89 2227 2629 LOC100193871 [Zea mays] &gt; gi|194694338|gb|ACF81253.1| unknown [Zea mays] ACN36503 unknown [Zea mays] 0.89 2228 2630 NP_001144520 hypothetical protein 0.89 2229 2631 LOC100277514 [Zea mays] &gt; gi|195643396|gb|ACG41166.1| hypothetical protein [Zea mays] ACN25444 unknown [Zea mays] 0.89 2230 2632 XP_002454376 hypothetical protein 0.83 2231 2633 SORBIDRAFT_04g029660 [Sorghum bicolor] &gt; gi|241934207|gb|EE507352.1| hypothetical protein SORBIDRAFT_04g029660 [Sorghum bicolor] 302-321 XP_002445712 hypothetical protein 1.00 2232 2634 SORBIDRAFT_07g024570 [Sorghum bicolor] &gt; gi|241942062|gb|EES15207.1| hypothetical protein SORBIDRAFT_07g024570 [Sorghum bicolor] NP_001148095 auxin-independent growth 0.95 2233 2635 promoter [Zea mays] &gt; gi|195615748|gb|ACG29704.1| auxin-independent growth promoter [Zea mays] BAD10226 putative auxin-independent 0.82 2234 2636 growth promoter [Oryza sativa Japonica Group] &gt; gi|50725540|dbj|BAD33009.1| putative auxin-independent growth promoter [Oryza sativa Japonica Group] XP_002460613 hypothetical protein 0.71 2235 2637 SORBIDRAFT_02g031900 [Sorghum bicolor] &gt; gi|241923990|gb|EER97134.1| hypothetical protein SORBIDRAFT_02g031900 [Sorghum bicolor] 151-170 NP_001144386 hypothetical protein 1.00 2236 2638 LOC100277314 [Zea mays] &gt; gi|195641382|gb|ACG40159.1| hypothetical protein [Zea mays] 781-800 NP_001151955 senescence-associated protein 15 1.00 2237 2639 [Zea mays] &gt; gi|195651331|gb|ACG45133.1| senescence-associated protein 15 [Zea mays] 46-65 XP_002465563 hypothetical protein 1.00 2238 2640 SORBIDRAFT_01g041190 [Sorghum bicolor] &gt; gi|241919417|gb|EER92561.1| hypothetical protein SORBIDRAFT_01g041190 [Sorghum bicolor] NP_001049540 Os03g0245700 [Oryza sativa 0.88 2239 2641 Japonica Group] &gt; gi|108707147|gb|ABF94942.1| senescence-associated protein 15, putative, expressed [Oryza sativa Japonica Group] &gt; gi|113548011|dbj|BAF11454.1| Os03g0245700 [Oryza sativa Japonica Group] &gt; gi|125543096|gb|EAY89235.1| hypothetical protein OsI_10732 [Oryza sativa Indica Group] &gt; gi|125585589|gb|EAZ26253.1| hypothetical protein OsJ_10120 [Oryza sativa Japonica Group] BAJ85769 predicted protein [Hordeum 0.86 2240 2642 vulgare subsp. vulgare] &gt; gi|326514266|dbj|BAJ92283.1| predicted protein [Hordeum vulgare subsp. vulgare] XP_002464724 hypothetical protein 0.76 2241 2643 SORBIDRAFT_01g025820 [Sorghum bicolor] &gt; gi|241918578|gb|EER91722.1| hypothetical protein SORBIDRAFT_01g025820 [Sorghum bicolor] AAC34858 senescence-associated protein 15 0.70 2242 2644 [Hemerocallis hybrid cultivar] EEC66585 hypothetical protein OsI_32794 0.74 2243 [Oryza sativa Indica Group] EEE50594 hypothetical protein OsJ_30776 0.74 2244 [Oryza sativa Japonica Group] NP_001064196 Os10g0158100 [Oryza sativa 0.73 2245 2645 Japonica Group] &gt; gi|255679225|dbj|BAF26110.2| Os10g0158100 [Oryza sativa Japonica Group] 400-419 NP_001149275 DNA polymerase epsilon subunit 1.00 2246 2646 3 [Zea mays] &gt; gi|195625970|gb|ACG34815.1| DNA polymerase epsilon subunit 3 [Zea mays] &gt; gi|195628668|gb|ACG36164.1| DNA polymerase epsilon subunit 3 [Zea mays] &gt; gi|223942345|gb|ACN25256.1| unknown [Zea mays] XP_002461994 hypothetical protein 0.71 2247 2647 SORBIDRAFT_02g012030 [Sorghum bicolor] &gt; gi|241925371|gb|EER98515.1| hypothetical protein SORBIDRAFT_02g012030 [Sorghum bicolor] 71-90 NP_001141681 hypothetical protein 1.00 2248 2648 LOC100273808 [Zea mays] &gt; gi|238908879|gb|ACF86850.2| unknown [Zea mays] 106-125 XP_002468642 hypothetical protein 1.00 2249 2649 SORBIDRAFT_01g049490 [Sorghum bicolor] &gt; gi|241922496|gb|EER95640.1| hypothetical protein SORBIDRAFT_01g049490 [Sorghum bicolor] ACG46521 F-box protein [Zea mays] 0.83 2250 2650 670-689 XP_002444258 hypothetical protein 1.00 2251 2651 SORBIDRAFT_07g019090 [Sorghum bicolor] &gt; gi|241940608|gb|EES13753.1| hypothetical protein SORBIDRAFT_07g019090 [Sorghum bicolor] NP_001131520 hypothetical protein 0.95 2252 2652 LOC100192859 [Zea mays] &gt; gi|194691750|gb|ACF79959.1| unknown [Zea mays] ACG32608 peroxisomal coenzyme A 0.95 2253 2653 diphosphatase NUDT7 [Zea mays] EAZ06644 hypothetical protein OsI_28893 0.74 2254 [Oryza sativa Indica Group] &gt; gi|125561356|gb|EAZ06804.1| hypothetical protein OsI_29048 [Oryza sativa Indica Group] EAZ42551 hypothetical protein OsJ_27117 0.74 2255 [Oryza sativa Japonica Group] EAZ06802 hypothetical protein OsI_29046 0.72 2256 [Oryza sativa Indica Group] NP_001061674 Os08g0375900 [Oryza sativa 0.72 2257 2654 Japonica Group] &gt; gi|40253324|dbj|BAD05258.1| putative phosphohydrolase [Oryza sativa Japonica Group] &gt; gi|40253358|dbj|BAD05290.1| putative phosphohydrolase [Oryza sativa Japonica Group] &gt; gi|113623643|dbj|BAF23588.1| Os08g0375900 [Oryza sativa Japonica Group] XP_002444259 hypothetical protein 0.71 2258 2655 SORBIDRAFT_07g019110 [Sorghum bicolor] &gt; gi|241940609|gb|EES13754.1| hypothetical protein SORBIDRAFT_07g019110 [Sorghum bicolor] 2195-2214 XP_002440313 hypothetical protein 1.00 2259 2656 SORBIDRAFT_09g029610 [Sorghum bicolor] &gt; gi|241945598|gb|EES18743.1| hypothetical protein SORBIDRAFT_09g029610 [Sorghum bicolor] ACG34981 glucose-1-phosphate 0.97 2260 2657 adenylyltransferase large subunit [Zea mays] &gt; gi|223948357|gb|ACN28262.1| unknown [Zea mays] NP_001056424 Os05g0580000 [Oryza sativa 0.93 2261 2658 Japonica Group] &gt; gi|51854319|gb|AAU10700.1| putative glucose-1-phosphate adenylyltransferase [Oryza sativa Japonica Group] &gt; gi|125553462|gb|EAY99171.1| hypothetical protein OsI_21129 [Oryza sativa Indica Group] &gt; gi|169244411|gb|ACA50479.1| ADP-glucose pyrophosphorylase large subunit [Oryza sativa Japonica Group] &gt; gi|215704797|dbj|BAG94825.1| unnamed protein product [Oryza sativa Japonica Group] &gt; gi|222632685|gb|EEE64817.1| hypothetical protein OsJ_19673 [Oryza sativa Japonica Group] &gt; gi|255676601|dbj|BAF18338.2| Os05g0580000 [Oryza sativa Japonica Group] &gt; gi|262344368|gb|ACY56044.1| ADP-glucose pyrophosphorylase large subunit [Oryza sativa Japonica Group] &gt; gi|262344370|gb|ACY56045.1| ADP-glucose pyrophosphorylase large subunit [Oryza sativa Japonica Group] &gt; gi|262344372|gb|ACY56046.1| ADP-glucose pyrophosphorylase large subunit [Oryza sativa Japonica Group] &gt; gi|262344374|gb|ACY56047.1| ADP-glucose pyrophosphorylase large subunit [Oryza sativa Japonica Group] &gt; gi|262344376|gb|ACY56048.1| ADP-glucose pyrophosphorylase large subunit [Oryza sativa Japonica Group] &gt; gi|262344378|gb|ACY56049.1| ADP-glucose pyrophosphorylase large subunit [Oryza sativa Japonica Group] &gt; gi|262344380|gb|ACY56050.1| ADP-glucose pyrophosphorylase large subunit [Oryza sativa Indica Group] &gt; gi|262344382|gb|ACY56051.1| ADP-glucose pyrophosphorylase large subunit [Oryza sativa Indica Group] &gt; gi|262344384|gb|ACY56052.1| ADP-glucose pyrophosphorylase large subunit [Oryza sativa Indica Group] &gt; gi|262344386|gb|ACY56053.1| ADP-glucose pyrophosphorylase large subunit [Oryza sativa Indica Group] &gt; gi|262344388|gb|ACY56054.1| ADP-glucose pyrophosphorylase large subunit [Oryza sativa Indica Group] &gt; gi|262344390|gb|ACY56055.1| ADP-glucose pyrophosphorylase large subunit [Oryza sativa Indica Group] &gt; gi|262344392|gb|ACY56056.1| ADP-glucose pyrophosphorylase large subunit [Oryza sativa Indica Group] &gt; gi|262344394|gb|ACY56057.1| ADP-glucose pyrophosphorylase large subunit [Oryza sativa Indica Group] BAA23490 ADP glucose pyrophosphorylase 0.92 2262 2659 large subunit [Oryza sativa Japonica Group] AAD39597 10A19I.12 [Oryza sativa Japonica 0.93 2263 2660 Group] P12299 RecName: Full = Glucose-1- 0.91 2264 phosphate adenylyltransferase large subunit, chloroplastic/amyloplastic; AltName: Full = ADP-glucose pyrophosphorylase; AltName: Full = ADP-glucose synthase; AltName: Full = AGPase S; AltName: Full = Alpha-D-glucose- 1-phosphate adenyl transferase; Flags: Precursor &gt; gi|995746|emb|CAA79980.1| ADP-glucose pyrophosphorylase large subunit [Triticum aestivum] &gt; gi|110729318|gb|ABG88200.1| ADP-glucose pyrophosphorylase large subunit [Triticum aestivum] P30524 RecName: Full = Glucose-1- 0.91 2265 phosphate adenylyltransferase large subunit 1, chloroplastic/amyloplastic; AltName: Full = ADP-glucose pyrophosphorylase; AltName: Full = ADP-glucose synthase; AltName: Full = AGPase S; AltName: Full = Alpha-D-glucose- 1-phosphate adenyl transferase; AltName: Full = BEPL; Flags: Precursor &gt; gi|1279513|emb|CAA47626.1| glucose-1-phosphate adenylyltransferase [Hordeum vulgare subsp. vulgare] &gt; gi|229610847|emb|CAX51355.1| large subunit of ADP-glucose pyrophosphorylase [Hordeum vulgare subsp. vulgare] &gt; gi|326527375|dbj|BAK04629.1| predicted protein [Hordeum vulgare subsp. vulgare] &gt; gi|326528409|dbj|BAJ93393.1| predicted protein [Hordeum vulgare subsp. vulgare] &gt; gi|326528511|dbj|BAJ93437.1| predicted protein [Hordeum vulgare subsp. vulgare] CAD98749 ADP-glucose pyrophosphorylase 0.89 2266 2661 large subunit [Triticum aestivum] NP_001105717 glucose-1-phosphate 0.92 2267 2662 adenylyltransferase large subunit 2, chloroplastic/amyloplastic [Zea mays] &gt;gi|707928|sp|p55234.1|GLG L2_MAIZE RecName: Full = Glucose-1-phosphate adenylyltransferase large subunit 2, chloroplastic/amyloplastic; AltName: Full = ADP-glucose pyrophosphorylase; AltName: AltName: Full = synthase AltName: Full = AGPase S; AltName: Full = Alpha-D-glucose- 1-phosphate adenyl transferase; Flags: Precursor &gt;gi|558365|emb|CAA86227.1| ADP-glucose pyrophosphorylase [Zea mays] 1909370A ADP glucose 0.87 2268 Predicted 154-171 XP_001545081 hypothetical protie BC1G_16418 1.00 2269 2663 siRNA [Botryotinia fuckeliana B05.10 ] 60837 &gt;gi|150854146|gb|EDN29338.1| hypothetical protein BCIG_(16418[) Botryotinia fuckeliana B05.10 629-646 XP_002441128 hypothetical protein 1.00 2270 2664 SORBIDRAFT_09g020930 [Sorghum bicolor] &gt:g1|241946413|gb|EES19558.1| hypothetical protein SORBIDRAFT_09g020930 [Sorghum bicolor] ACR35990 unknown [Zea mays] 0.91 2271 2665 NP_001152387 LOC100286027 [Zea mays] 0.92 2272 2666 &gt;gi|195655783|gb|ACG47359.1| secondary cell wall-related glycosyltransferase family 8 [Zea mays] BAJ85213 predicted protein [Hordeum 0.86 2273 2667 vulgare subsp. vulgare] &gt;gi|326515442|dbj|BAK03634.1| predicted protein [Hordeum vulgare subsp. vulgare] AAS90653 putative glycogenin [Oryza sativa 0.84 2274 2668 Japonica Group] EEC79261 hypothetical protein OsI_20036 0.84 2275 [Oryza sativa Indica Group] EEE63788 hypothetical protein OsJ_18611 0.83 2276 [Oryza sativa Japonica Group] NP_(—00104491) Os01g0880200 [Oryza sativa 0.84 2277 2669 Japonica Group] &gt;gi|56784626|dbj|BAD81673.1| glycogenin-like protein [Oryza sativa Japonica Group] &gt; gi|113534522|dbj|BAF06905.1| Os01g0880200 [Oryza sativa Japonica Group] EEC71899 hypothetical protein OsI_04661 0.84 2278 [Oryza sativa Indica Group] BAJ87677 predicted protein [Hordeum 0.82 2279 2670 vulgare subsp. vulgare] 687-704 EEC83357 hypothetical protein OsI_28763 1.00 2280 [Oryza sativa Indica Group] &gt; gi|222623167|gb|EEE57299.1| hypothetical protein OsJ_07374 [Oryza sativa Japonica Group] NP_001060995 Os08g0150800 [Oryza sativa 0.94 2281 2671 Japonica Group] &gt; gi|37573038|dbj|BAC98550.1| putative tyrosyl-tRNA synthetase [Oryza sativa Japonica Group] &gt; gi|113622964|dbj|BAF22909.1| Os08g0150800 [Oryza sativa Japonica Group] &gt; gi|222639916|gb|EEE68048.1| hypothetical protein OsJ_26046 [Oryza sativa Japonica Group] ACF82652 unknown [Zea mays] 0.87 2282 2672 NP_001149217 LOC100282839 [Zea mays] 0.86 2283 2673 &gt; gi|195625536|gb|ACG34598.1| tyrosyl-tRNA synthetase [Zea mays] NP_001148699 tyrosyl-tRNA synthetase [Zea 0.83 2284 2674 mays] &gt; gi|195621482|gb|ACG32571.1| tyrosyl-tRNA synthetase [Zea mays] BAK05692 predicted protein [Hordeum 0.83 2285 2675 vulgare subsp. vulgare] &gt; gi|326527699|dbj|BAK08124.1| predicted protein [Hordeum vulgare subsp. vulgare] &gt; gi|326533322|dbj|BAJ93633.1| predicted protein [Hordeum vulgare subsp. vulgare] CAA71881 Tyrosyl-tRNA synthetase 0.69 2286 2676 [Nicotiana tabacum] XP_002517485 tyrosyl-tRNA synthetase, putative 0.71 2287 2677 [Ricinus communis] &gt; gi|223543496|gb|EEF45027.1| tyrosyl-tRNA synthetase, putative [Ricinus communis] XP_002274369 PREDICTED: hypothetical 0.71 2288 2678 protein [Vitis vinifera] &gt; gi|297742518|emb|CBI34667.3| unnamed protein product [Vitis vinifera] CAN65657 hypothetical protein 0.71 2289 2679 VITISV_000951 [Vitis vinifera] 796-813 ACG35054 THO complex subunit 4 [Zea 1.00 2290 2680 mays] NP_001146734 hypothetical protein 0.99 2291 2681 LOC100280336 [Zea mays] &gt; gi|194704900|gb|ACF86534.1| unknown [Zea mays] &gt; gi|219888533|gb|ACL54641.1| unknown [Zea mays] XP_002438180 hypothetical protein 0.87 2292 2682 SORBIDRAFT_10g009240 [Sorghum bicolor] &gt; gi|241916403|gb|EER89547.1| hypothetical protein SORBIDRAFT_10g009240 [Sorghum bicolor] 310-327 NP_001168358 hypothetical protein 1.00 2293 2683 LOC100382126 [Zea mays] &gt; gi|223947741|gb|ACN27954.1| unknown [Zea mays] BAD54151 putative poly(A) polymerase 0.81 2294 [Oryza sativa Japonica Group] BAJ95542 predicted protein [Hordeum 0.80 2295 2684 vulgare subsp. vulgare] NP_001174846 Os06g0558700 [Oryza sativa 0.80 2296 2685 Japonica Group] &gt; gi|255677141|dbj|BAH93574.1| Os06g0558700 [Oryza sativa Japonica Group] AAW68015 putative polynucleotide 0.80 2297 2686 adenylyltransferase [Oryza sativa Indica Group] EAZ37348 hypothetical protein OsJ_21686 0.78 2298 [Oryza sativa Japonica Group] EAZ01335 hypothetical protein OsI_23369 0.78 2299 [Oryza sativa Indica Group] 576-593 XP_002466644 hypothetical protein 1.00 2300 2687 SORBIDRAFT_01g011560 [Sorghum bicolor] &gt; gi|241920498|gb|EER93642.1| hypothetical protein SORBIDRAFT_01g011560 [Sorghum bicolor] NP_001169332 hypothetical protein 0.96 2301 2688 LOC100383199 [Zea mays] &gt; gi|224028751|gb|ACN33451.1| unknown [Zea mays] NP_001130678 hypothetical protein 0.95 2302 2689 LOC100191781 [Zea mays] &gt; gi|194688930|gb|ACF78549.1| unknown [Zea mays] EEC81302 hypothetical protein OsI_24438 0.87 2303 [Oryza sativa Indica Group] &gt; gi|222636212|gb|EEE66344.1| hypothetical protein OsJ_22634 [Oryza sativa Japonica Group] BAJ99110 predicted protein [Hordeum 0.86 2304 2690 vulgare subsp. vulgare] BAJ86220 predicted protein [Hordeum 0.75 2305 2691 vulgare subsp. vulgare] NP_001063757 Os09g0531900 [Oryza sativa 0.74 2306 2692 Japonica Group] &gt; gi|52075938|dbj|BAD46018.1| glycosyl transferase family 8 protein-like [Oryza sativa Japonica Group] &gt; gi|52077221|dbj|BAD46265.1| glycosyl transferase family 8 protein-like [Oryza sativa Japonica Group] &gt; gi|113631990|dbj|BAF25671.1| Os09g0531900 [Oryza sativa Japonica Group] &gt; gi|222641970|gb|EEE70102.1| hypothetical protein OsJ_30110 [Oryza sativa Japonica Group] EEC84934 hypothetical protein OsI_32147 0.74 2307 [Oryza sativa Indica Group] XP_002462717 hypothetical protein 0.75 2308 2693 SORBIDRAFT_02g030820 [Sorghum bicolor] &gt; gi|241926094|gb|EER99238.1| hypothetical protein SORBIDRAFT_02g030820 [Sorghum bicolor] CBI38820 unnamed protein product [Vitis 0.72 2309 vinifera] Predicted 70-89 XP_002446795 hypothetical protein 1.00 2310 2694 siRNA SORBIDRAFT_06g022830 61382 [Sorghum bicolor] &gt; gi|241937978|gb|EES11123.1| hypothetical protein SORBIDRAFT_06g022830 [Sorghum bicolor] NP_001143685 hypothetical protein 0.86 2311 2695 LOC100276413 [Zea mays] &gt; gi|195624570|gb|ACG34115.1| hypothetical protein [Zea mays] ACN29321 unknown [Zea mays] 0.86 2312 2696 NP_001053334 Os04g0520900 [Oryza sativa 0.72 2313 2697 Japonica Group] &gt; gi|21741854|emb|CAD41444.1| OSJNBa0019D11.15 [Oryza sativa Japonica Group] &gt; gi|13564905|dbj|BAF15248.1| Os04g0520900 [Oryza sativa Japonica Group] &gt; gi|116310729|emb|CAH67525.1| OSIGBa0131L05.6 [Oryza sativa Indica Group] &gt; gi|125549050|gb|EAY94872.1| hypothetical protein OsI_16672 [Oryza sativa Indica Group] &gt; gi|125591012|gb|EAZ31362.1| hypothetical protein OsJ_15488 [Oryza sativa Japonica Group] &gt; gi|215697100|dbj|BAG91094.1| unnamed protein product [Oryza sativa Japonica Group] 682-701 NP_001143685 hypothetical protein 1.00 2314 2698 LOC100276413 [Zea mays] &gt; gi|195624570|gb|ACG34115.1| hypothetical protein [Zea mays] ACN29321 unknown [Zea mays] 0.95 2315 2699 XP_002446795 hypothetical protein 0.90 2316 2700 SORBIDRAFT_06g022830 [Sorghum bicolor] &gt; gi|241937978|gb|EES11123.1| hypothetical protein SORBIDRAFT_06g022830 [Sorghum bicolor] NP_001053334 Os04g0520900 [Oryza sativa 0.70 2317 2701 Japonica Group] &gt; gi|21741854|emb|CAD41444.1| OSJNBa0019D11.15 [Oryza sativa Japonica Group] &gt; gi|113564905|dbj|BAF15248.1| Os04g0520900 [Oryza sativa Japonica Group] &gt; gi|116310729|emb|CAH67525.1| OSIGBa0131L05.6 [Oryza sativa Indica Group] &gt; gi|125549050|gb|EAY94872.1| hypothetical protein OsI_16672 [Oryza sativa Indica Group] &gt; gi|125591012|gb|EAZ31362.1| hypothetical protein OsJ_15488 [Oryza sativa Japonica Group] &gt; gi|215697100|dbj|BAG91094.1| unnamed protein product [Oryza sativa Japonica Group] Predicted 237-254 XP_002459573 hypothetical protein 1.00 2318 2702 siRNA SORBIDRAFT_02g006770 60188 [Sorghum bicolor] &gt; gi|241922950|gb|EER96094.1| hypothetical protein SORBIDRAFT_02g006770 [Sorghum bicolor] NP_001150031 nucleic acid binding protein [Zea 0.98 2319 2703 mays] &gt; gi|194699002|gb|ACF83585.1| unknown [Zea mays] &gt; gi|195636208|gb|ACG37572.1| nucleic acid binding protein [Zea mays] NP_001140438 hypothetical protein 0.96 2320 2704 LOC100272497 [Zea mays] &gt; gi|194699514|gb|ACF83841.1| unknown [Zea mays] NP_001059216 Os07g0227400 [Oryza sativa 0.91 2321 2705 Japonica Group] &gt; gi|24060154|dbj|BAC21599.1| KH domain-like protein [Oryza sativa Japonica Group] &gt; gi|113610752|dbj|BAF21130.1| Os07g0227400 [Oryza sativa Japonica Group] &gt; gi|215697533|dbj|BAG91527.1| unnamed protein product [Oryza sativa Japonica Group] EAZ39172 hypothetical protein OsJ_23597 0.91 2322 [Oryza sativa Japonica Group] &gt; gi|218199326|gb|EEC81753.1| hypothetical protein OsI_25419 [Oryza sativa Indica Group] NP_001148920 nucleic acid binding protein [Zea 0.85 2323 2706 mays] &gt; gi|195623320|gb|ACG33490.1| nucleic acid binding protein [Zea mays] XP_002466215 hypothetical protein 0.85 2324 2707 SORBIDRAFT_01g003680 [Sorghum bicolor] &gt; gi|241920069|gb|EER93213.1| hypothetical protein SORBIDRAFT_01g003680 [Sorghum bicolor] NP_001051685 Os03g0815700 [Oryza sativa 0.86 2325 2708 Japonica Group] &gt; gi|75226290|sp|Q75GR5.1|SPIN1_ORYSJ RecName: Full = KH domain-containing protein SPIN1; AltName: Full = SPL11-interacting protein 1 &gt; gi|37718879|gb|AAR01750.1| expressed protein [Oryza sativa Japonica Group] &gt; gi|108711745|gb|ABF99540.1| KH domain-containing protein, putative, expressed [Oryza sativa Japonica Group] &gt; gi|113550156|dbj|BAF13599.1| Os03g0815700 [Oryza sativa Japonica Group] &gt; gi|215694514|dbj|BAG89507.1| unnamed protein product [Oryza sativa Japonica Group] &gt; gi|218193980|gb|EEC76407.1| hypothetical protein OsI_14057 [Oryza sativa Indica Group] &gt; gi|222626037|gb|EEE60169.1| hypothetical protein OsJ_13097 [Oryza sativa Japonica Group] NP_001130116 hypothetical protein 0.84 2326 2709 LOC100191210 [Zea mays] &gt; gi|194688334|gb|ACF78251.1| unknown [Zea mays] BAJ96236 predicted protein [Hordeum 0.83 2327 2710 vulgare subsp. vulgare] 362-379 XP_002448500 hypothetical protein 1.00 2328 2711 SORBIDRAFT_06g028030 [Sorghum bicolor] &gt; gi|241939683|gb|EES12828.1| hypothetical protein SORBIDRAFT_06g028030 [Sorghum bicolor] NP_001148728 protein phosphatase 2C [Zea 0.93 2329 2712 mays] &gt; gi|195621678|gb|ACG32669.1| protein phosphatase 2C [Zea mays] &gt; gi|238007398|gb|ACR34734.1| unknown [Zea mays] NP_001053823 Os04g0609600 [Oryza sativa 0.91 2330 2713 Japonica Group] &gt; gi|122240832|sp|Q0JAA0.1|P2C44_ORYSJ RecName: Full = Probable protein phosphatase 2C 44; Short = OsPP2C44 &gt; gi|113565394|dbj|BAF15737.1| Os04g0609600 [Oryza sativa Japonica Group] 5 [Oryza sativa Japonica Group] EEC71777 hypothetical protein OsI_04389 0.80 2339 [Oryza sativa Indica Group] BAJ99773 predicted protein [Hordeum 0.78 2340 2721 vulgare subsp. vulgare] XP_002458747 hypothetical protein 0.78 2341 2722 SORBIDRAFT_03g039530 [Sorghum bicolor] &gt; gi|241930722|gb|EES03867.1| hypothetical protein SORBIDRAFT_03g039530 [Sorghum bicolor] AAC04576 putative high-pI laccase [Oryza 0.79 2342 2723 sativa Japonica Group] 725-742 CAE03557 OSJNBa0085I10.2 [Oryza sativa 1.00 2343 2724 Japonica Group] &gt; gi|90265077|emb|CAH67750.1| H0702G05.9 [Oryza sativa Indica Group] NP_001053823 Os04g0609600 [Oryza sativa 1.00 2344 2725 Japonica Group] &gt; gi|122240832|sp|Q0JAA0.1|P2C44_ORYSJ RecName: Full = Probable protein phosphatase 2C 44; Short = OsPP2C44 &gt; gi|113565394|dbj|BAF15737.1| Os04g0609600 [Oryza sativa Japonica Group] &gt; gi|218195541|gb|EEC77968.1| hypothetical protein OsI_17335 [Oryza sativa Indica Group] &gt; gi|222629521|gb|EEE61653.1| hypothetical protein OsJ_16105 [Oryza sativa Japonica Group] XP_002448500 hypothetical protein 0.91 2345 2726 SORBIDRAFT_06g028030 [Sorghum bicolor] &gt; gi|241939683|gb|EES12828.1| hypothetical protein SORBIDRAFT_06g028030 [Sorghum bicolor] NP_001148728 protein phosphatase 2C [Zea 0.90 2346 2727 mays] &gt; gi|195621678|gb|ACG32669.1| protein phosphatase 2C [Zea mays] &gt; gi|238007398|gb|ACR34734.1| unknown [Zea mays] CAE03658 OSJNBa0060NO3.23 [Oryza 0.75 2347 2728 sativa Japonica Group] XP_002274944 PREDICTED: hypothetical 0.74 2348 2729 protein isoform 1 [Vitis vinifera] &gt; gi|297745124|emb|CB138963.3| unnamed protein product [Vitis vinifera] XP_002514493 protein phosphatase 2c, putative 0.76 2349 2730 [Ricinus communis] &gt; gi|223546392|gb|EEF47893.1| protein phosphatase 2c, putative [Ricinus communis] XP_002274981 PREDICTED: hypothetical 0.71 2350 2731 protein isoform 2 [Vitis vinifera] 879-896 NP_001054756 Os05g0168700 [Oryza sativa 1.00 2351 2732 Japonica Group] &gt; gi|53982148|gb|AAV25244.1| putative phosphate translocator [Oryza sativa Japonica Group] &gt; gi|113578307|dbj|BAF16670.1| Os05g0168700 [Oryza sativa Japonica Group] &gt; gi|215686739|dbj|BAG89589.1| unnamed protein product [Oryza sativa Japonica Group] &gt; gi|218196167|gb|EEC78594.1| hypothetical protein OsI_18612 [Oryza sativa Indica Group] &gt; gi|222630341|gb|EEE62473.1| hypothetical protein OsJ_17270 [Oryza sativa Japonica Group] BAB41206 putative glucose-6- 0.99 2352 2733 phosphate/phosphate-tranlocat or [Oryza sativa (japonica cultivar- group)] ACN32013 unknown [Zea mays] 0.96 2353 2734 XP_002440680 hypothetical protein 0.96 2354 2735 SORBIDRAFT_09g005010 [Sorghum bicolor] &gt; gi|241945965|gb|EES19110.1| hypothetical protein SORBIDRAFT_09g005010 [Sorghum bicolor] NP_001142411 hypothetical protein 0.95 2355 2736 LOC100274586 [Zea mays] &gt; gi|194707946|gb|ACF88057.1| unknown [Zea mays] &gt; gi|194708688|gb|ACF88428.1| unknown [Zea mays] &gt; gi|195620476|gb|ACG32068.1| integral membrane protein like [Zea mays] BAK03308 predicted protein [Hordeum 0.95 2356 2737 vulgare subsp. vulgare] NP_001148556 integral membrane protein like 0.87 2357 2738 [Zea mays] &gt; gi|195620390|gb|ACG32025.1| integral membrane protein like [Zea mays] NP_001042121 Os01g0167500 [Oryza sativa 0.92 2358 2739 Japonica Group] &gt; gi|13486667|dbj|BAB39904.1| P0028E10.8 [Oryza sativa Japonica Group] &gt; gi|15528768|dbj|BAB64810.1| putative glucose-6- phosphate/phosphate-tranlocator [Oryza sativa Japonica Group] &gt; gi|20804811|dbj|BAB92494.1| putative glucose-6- phosphate/phosphate-tranlocator [Oryza sativa Japonica Group] &gt; gi|113531652|dbj|BAF04035.1| Os01g0167500 [Oryza sativa Japonica Group] &gt; gi|215767361|dbj|BAG99589.1| unnamed protein product [Oryza sativa Japonica Group] &gt; gi|218187579|gb|EEC70006.1| hypothetical protein OsI_00550 [Oryza sativa Indica Group] &gt; gi|222617800|gb|EEE53932.1| hypothetical protein OsJ_00515 [Oryza sativa Japonica Group] NP_001142171 hypothetical protein 0.93 2359 2740 LOC100274338 [Zea mays] &gt; gi|194707458|gb|ACF87813.1| unknown [Zea mays] ACN34739 unknown [Zea mays] 0.92 2360 2741 130-147 XP_002448782 hypothetical protein 1.00 2361 2742 SORBIDRAFT_06g033075 [Sorghum bicolor] &gt; gi|241939965|gb|EES13110.1| hypothetical protein SORBIDRAFT_06g033075 [Sorghum bicolor] NP_001151057 LOC100284690 [Zea mays] 0.92 2362 2743 &gt; gi|195643970|gb|ACG41453.1| ATP binding protein [Zea mays] EAZ32429 hypothetical protein OsJ_16639 0.82 2363 [Oryza sativa Japonica Group] NP_001054279 Os04g0679200 [Oryza sativa 0.82 2364 2744 Japonica Group] &gt; gi|38344039|emb|CAE05726.2| OSJNBb0017I01.6 [Oryza sativa Japonica Group] &gt; gi|113565850|dbj|BAF16193.1| Os04g0679200 [Oryza sativa Japonica Group] &gt; gi|215712397|dbj|BAG94524.1| unnamed protein product [Oryza sativa Japonica Group] &gt; gi|215740866|dbj|BAG97022.1| unnamed protein product [Oryza sativa JaponicaGroup] CAJ86254 H0801D08.12 [Oryza sativa 0.82 2365 2745 Indica Group] &gt; gi|125550244|gb|EAY96066.1| hypothetical protein OsI_17939 [Oryza sativa Indica Group] BAJ93433 predicted protein [Hordeum 0.80 2366 2746 vulgare subsp. vulgare] 99-116 XP_002452000 hypothetical protein 1.00 2367 2747 SORBIDRAFT_04g014390 [Sorghum bicolor] &gt; gi|241931831|gb|EES04976.1| hypothetical protein SORBIDRAFT_04g014390 [Sorghum bicolor] ACR36123 unknown [Zea mays] 0.93 2368 2748 NP_001145206 hypothetical protein 0.92 2369 2749 LOC100278461 [Zea mays] &gt; gi|195652631|gb|ACG45783.1| hypothetical protein [Zea mays] NP_001046716 Os02g0329300 [Oryza sativa 0.86 2370 2750 Japonica Group] &gt; gi|46389946|dbj|BAD15798.1| unknown protein [Oryza sativa Japonica Group] &gt; gi|113536247|dbj|BAF08630.1| Os02g0329300 [Oryza sativa Japonica Group] &gt; gi|222622747|gb|EEE56879.1| hypothetical protein OsJ_06518 [Oryza sativa Japonica Group] EEC73063 hypothetical protein OsI_07020 0.85 2371 [Oryza sativa Indica Group] BAK07073 predicted protein [Hordeum 0.81 2372 2751 vulgare subsp. vulgare] 736-753 ACN28855 unknown [Zea mays] 1.00 2373 2752 NP_001105875 putative laccase [Zea mays] 0.99 2374 2753 &gt; gi|84618781|emb|CAJ30500.1| putative laccase [Zea mays] NP_001146658 hypothetical protein 0.93 2375 2754 LOC100280258 [Zea mays] &gt; gi|219888209|gb|ACL54479.1| unknown [Zea mays] XP_002458746 hypothetical protein 0.93 2376 2755 SORBIDRAFT_03g039520 [Sorghum bicolor] &gt; gi|241930721|gb|EES03866.1| hypothetical protein SORBIDRAFT_03g039520 [Sorghum bicolor] NP_001044772 Os01g0842400 [Oryza sativa 0.81 2377 2756 Japonica Group] &gt; gi|75321217|sp|Q5N9X2.1|LAC4_ORYSJ RecName: Full = Laccase-4; AltName: Full = Benzenediol:oxygen oxidoreductase 4; AltName: Full = Diphenol oxidase 4; AltName: Full = Urishiol oxidase 4; Flags: Precursor &gt; gi|56784239|dbj|BAD81734.1| putative laccase LAC5-6 [Oryza sativa Japonica Group] &gt; gi|113534303|dbj|BAF06686.1| Os01g0842400 [Oryza sativa Japonica Group] &gt; gi|215697155|dbj|BAG91149.1| unnamed protein product [Oryza sativa Japonica Group] EAZ14115 hypothetical protein OsJ_04039 0.81 2378 [Oryza sativa Japonica Group] EEC71777 hypothetical protein OsI_04389 0.80 2379 [Oryza sativa Indica Group] BAJ99773 predicted protein [Hordeum 0.78 2380 2757 vulgare subsp. vulgare] XP_002458747 hypothetical protein 0.78 2381 2758 SORBIDRAFT_03g039530 [Sorghum bicolor] &gt; gi|241930722|gb|EES03867.1| hypothetical protein SORBIDRAFT_03g039530 [Sorghum bicolor] AAC04576 putative high-pI laccase [Oryza 0.79 2382 2759 sativa Japonica Group] 0-17 NP_001132686 hypothetical protein 1.00 2383 2760 LOC100194164 [Zea mays] &gt; gi|194695090|gb|ACF81629.1| unknown [Zea mays] XP_002457397 hypothetical protein 0.81 2384 2761 SORBIDRAFT_03g006690 [Sorghum bicolor] &gt; gi|241929372|gb|EES02517.1| hypothetical protein SORBIDRAFT_03g006690 [Sorghum bicolor] 1000-1017 NP_001136742 hypothetical protein 1.00 2385 2762 LOC100216883 [Zea mays] &gt; gi|194696868|gb|ACF82518.1| unknown [Zea mays] &gt; gi|195648274|gb|ACG43605.1| monoglyceride lipase [Zea mays] XP_002450081 hypothetical protein 0.80 2386 2763 SORBIDRAFT_05g000200 [Sorghum bicolor] &gt; gi|241935924|gb|EES09069.1| hypothetical protein SORBIDRAFT_05g000200 [Sorghum bicolor] XP_002442631 hypothetical protein 0.80 2387 2764 SORBIDRAFT_08g000200 [Sorghum bicolor] &gt; gi|241943324|gb|EES16469.1| hypothetical protein SORBIDRAFT_08g000200 [Sorghum bicolor] 296-313 XP_002440680 hypothetical protein 1.00 2388 2765 SORBIDRAFT_09g005010 [Sorghum bicolor] &gt; gi|241945965|gb|EES19110.1| hypothetical protein SORBIDRAFT_09g005010 [Sorghum bicolor] ACN32013 unknown [Zea mays] 0.99 2389 2766 NP_001142411 hypothetical protein 0.98 2390 2767 LOC100274586 [Zea mays] &gt; gi|194707946|gb|ACF88057.1| unknown [Zea mays] &gt; gi|194708688|gb|ACF88428.1| unknown [Zea mays] &gt; gi|195620476|gb|ACG32068.1| integral membrane protein like [Zea mays] NP_001054756 Os05g0168700 [Oryza sativa 0.96 2391 2768 Japonica Group] &gt; gi|53982148|gb|AAV25244.1| putative phosphate translocator [Oryza sativa Japonica Group] &gt; gi|113578307|dbj|BAF16670.1| Os05g0168700 [Oryza sativa Japonica Group] &gt; gi|215686739|dbj|BAG89589.1| unnamed protein product [Oryza sativa Japonica Group] &gt; gi|218196167|gb|EEC78594.1| hypothetical protein OsI_18612 [Oryza sativa Indica Group] &gt; gi|222630341|gb|EEE62473.1| hypothetical protein OsJ_17270 [Oryza sativa Japonica Group] BAB41206 putative glucose-6- 0.95 2392 2769 phosphate/phosphate-tranlocat or [Oryza sativa (japonica cultivar- group)] BAK03308 predicted protein [Hordeum 0.94 2393 2770 vulgare subsp. vulgare] NP_001148556 integral membrane protein like 0.90 2394 2771 [Zea mays] &gt; gi|195620390|gb|ACG32025.1| integral membrane protein like [Zea mays] NP_001042121 Os01g0167500 [Oryza sativa 0.92 2395 2772 Japonica Group] &gt; gi|13486667|dbj|BAB39904.1| P0028E10.8 [Oryza sativa Japonica Group] &gt; gi|15528768|dbj|BAB64810.1| putative glucose-6- phosphate/phosphate-tranlocator [Oryza sativa Japonica Group] &gt; gi|20804811|dbj|BAB92494.1| putative glucose-6- phosphate/phosphate-tranlocator [Oryza sativa Japonica Group] &gt; gi|113531652|dbj|BAF04035.1| Os01g0167500 [Oryza sativa Japonica Group] &gt; gi|215767361|dbj|BAG99589.1| unnamed protein product [Oryza sativa Japonica Group] &gt; gi|218187579|gb|EEC70006.1| hypothetical protein OsI_00550 [Oryza sativa Indica Group] &gt; gi|222617800|gb|EEE53932.1| hypothetical protein OsJ_00515 [Oryza sativa Japonica Group] NP_001142171 hypothetical protein 0.92 2396 2773 LOC100274338 [Zea mays] &gt; gi|194707458|gb|ACF87813.1| unknown [Zea mays] ACN34739 unknown [Zea mays] 0.91 2397 2774 1638-1655 NP_001183153 hypothetical protein 1.00 2398 2775 LOC100501523 [Zea mays] &gt; gi|238009674|gb|ACR35872.1| unknown [Zea mays] 1133-1150 NP_001146658 hypothetical protein 1.00 2399 2776 LOC100280258 [Zea mays] &gt; gi|219888209|gb|ACL54479.1| unknown [Zea mays] NP_001105875 putative laccase [Zea mays] 0.95 2400 2777 &gt; gi|84618781|emb|CAJ30500.1| putative laccase [Zea mays] ACN28855 unknown [Zea mays] 0.92 2401 2778 XP_002458746 hypothetical protein 0.91 2402 2779 SORBIDRAFT_03g039520 [Sorghum bicolor] &gt; gi|241930721|gb|EES03866.1| hypothetical protein SORBIDRAFT_03g039520 [Sorghum bicolor] NP_001044772 Os01g0842400 [Oryza sativa 0.80 2403 2780 Japonica Group] &gt; gi|75321217|sp|Q5N9X2.1|LAC4_ORYSJ RecName: Full = Laccase-4; AltName: Full = Benzenediol:oxygen oxidoreductase 4; AltName: Full = Diphenol oxidase 4; AltName: Full = Urishiol oxidase 4; Flags: Precursor &gt; gi|56784239|dbj|BAD81734.1| putative laccase LAC5-6 [Oryza sativa Japonica Group] &gt; gi|113534303|dbj|BAF06686.1| Os01g0842400 [Oryza sativa Japonica Group] &gt; gi|215697155|dbj|BAG91149.1| unnamed protein product [Oryza sativa Japonica Group] EAZ14115 hypothetical protein OsJ_04039 0.80 2404 [Oryza sativa Japonica Group] EEC71777 hypothetical protein OsI_04389 0.80 2405 [Oryza sativa Indica Group] BAJ99773 predicted protein [Hordeum 0.78 2406 2781 vulgare subsp. vulgare] AAC04576 putative high-pI laccase [Oryza 0.78 2407 2782 sativa Japonica Group] XP_002458747 hypothetical protein 0.77 2408 2783 SORBIDRAFT_03g039530 [Sorghum bicolor] &gt; gi|241930722|gb|EES03867.1| hypothetical protein SORBIDRAFT_03g039530 [Sorghum bicolor] 574-591 NP_001053823 Os04g0609600 [Oryza sativa 1.00 2409 2784 Japonica Group] &gt; gi|122240832|sp|Q0JAA0.1|P2C44_ORYSJ RecName: Full = Probable protein phosphatase 2C 44; Short = OsPP2C44 &gt; gi|113565394|dbj|BAF15737.1| Os04g0609600 [Oryza sativa Japonica Group] &gt; gi|218195541|gb|EEC77968.1| hypothetical protein OsI_17335 [Oryza sativa Indica Group] &gt; gi|222629521|gb|EEE61653.1| hypothetical protein OsJ_16105 [Oryza sativa Japonica Group] CAE03557 OSJNBa0085I10.2 [Oryza sativa 0.99 2410 2785 Japonica Group] &gt; gi|90265077|emb|CAH67750.1| H0702G05.9 [Oryza sativa Indica Group] XP_002448500 hypothetical protein 0.91 2411 2786 SORBIDRAFT_06g028030 [Sorghum bicolor] &gt; gi|241939683|gb|EES12828.1| hypothetical protein SORBIDRAFT_06g028030 [Sorghum bicolor] NP_001148728 protein phosphatase 2C [Zea 0.90 2412 2787 mays] &gt; gi|195621678|gb|ACG32669.1| protein phosphatase 2C [Zea mays] &gt; gi|238007398|gb|ACR34734.1| unknown [Zea mays] CAE03658 OSJNBa0060N03.23 [Oryza 0.74 2413 2788 sativa Japonica Group] XP_002274944 PREDICTED: hypothetical 0.73 2414 2789 protein isoform 1 [Vitis vinifera] &gt; gi|297745124|emb|CBI38963.3| unnamed protein product [Vitis vinifera] XP_002514493 protein phosphatase 2c, putative 0.75 2415 2790 [Ricinus communis] &gt; gi|223546392|gb|EEF47893.1| protein phosphatase 2c, putative [Ricinus communis] XP_002274981 PREDICTED: hypothetical 0.70 2416 2791 protein isoform 2 [Vitis vinifera] 1034-1051 NP_001170532 hypothetical protein 1.00 2417 2792 LOC100384546 [Zea mays] &gt; gi|238005884|gb|ACR33977.1| unknown [Zea mays] XP_002438672 hypothetical protein 0.88 2418 2793 SORBIDRAFT_10g024080 [Sorghum bicolor] &gt; gi|241916895|gb|EER90039.1| hypothetical protein SORBIDRAFT_10g024080 [Sorghum bicolor] EAZ01659 hypothetical protein OsI_23694 0.72 2419 [Oryza sativa Indica Group] EAZ37629 hypothetical protein OsJ_21963 0.72 2420 [Oryza sativa Japonica Group] BAD35523 unknown protein [Oryza sativa 0.72 2421 Japonica Group] &gt; gi|51090952|dbj|BAD35555.1| unknown protein [Oryza sativa Japonica Group] 1728-1745 AAW66346 YZ1 [Zea luxurians] 1.00 2422 2794 AAW66344 YZ1 [Zea mays subsp. mexicana] 0.99 2423 2795 AAW66348 YZ1 [Zea mays subsp. 0.99 2424 2796 parviglumis] AAM22634 YZ1 [Zea mays] 0.99 2425 2797 AAM21160 YZ1 [Zea mays] 0.98 2426 2798 ABB29302 YZ1 [Zea mays] 0.91 2427 2799 &gt; gi|78172241|gb|ABB29304.1| YZ1 [Zea mays] NP_001043656 Os01g0633400 [Oryza sativa 0.71 2428 2800 Japonica Group] &gt; gi|55296003|dbj|BAD68894.1| putative YZ1 [Oryza sativa Japonica Group] &gt; gi|113533187|dbj|BAF05570.1| Os01g0633400 [Oryza sativa Japonica Group] &gt; gi|215766352|dbj|BAG98580.1| unnamed protein product [Oryza sativa Japonica Group] XP_002456014 hypothetical protein 0.77 2429 2801 SORBIDRAFT_03g028870 [Sorghum bicolor] &gt; gi|241927989|gb|EES01134.1| hypothetical protein SORBIDRAFT_03g028870 [Sorghum bicolor] EAY75077 hypothetical protein OsI_02971 0.71 2430 [Oryza sativa Indica Group] Predicted 689-710 XP_002443139 hypothetical protein 1.00 2431 2802 siRNA SORBIDRAFT_08g011100 59659 [Sorghum bicolor] &gt; gi|241943832|gb|EES16977.1| hypothetical protein SORBIDRAFT_08g011100 [Sorghum bicolor] NP_001169596 hypothetical protein 0.94 2432 2803 LOC100383477 [Zea mays] &gt; gi|224030279|gb|ACN34215.1| unknown [Zea mays] NP_001066586 Os12g0285600 [Oryza sativa 0.83 2433 2804 Japonica Group] &gt; gi|77554686|gb|ABA97482.1| Oxysterol-binding protein, expressed [Oryza sativa Japonica Group] &gt; gi|113649093|dbj|BAF29605.1| Os12g0285600 [Oryza sativa Japonica Group] &gt; gi|215697261|dbj|BAG91255.1| unnamed protein product [Oryza sativa Japonica Group] &gt; gi|222616918|gb|EEE53050.1| hypothetical protein OsJ_35782 [Oryza sativa Japonica Group] EEC69105 hypothetical protein OsI_38013 0.81 2434 [Oryza sativa Indica Group] CBI16832 unnamed protein product [Vitis 0.71 2435 vinifera] XP_002282089 PREDICTED: similar to 0.72 2436 2805 oxysterol-binding family protein [Vitis vinifera] Table 7: Provided are the target Genes of siRNAs Associated with Increased NUE (Table 3) along with their GenBank Accession numbers and sequence identifiers (SEQ ID NO:). “bind” = binding; “pos” = position; “hom” = homologue; “p.p.” = polypeptide; “p.n.” = polynucleotide.

TABLE 8 Target genes of down regulated siRNA sequences associated with NUE (Table 4) p.p. p.n. Mir Hom. SEQ SEQ Mir Bind. NCBI ID ID name Pos. Accession Annotation/Organism Identity NO: NO: Predicted 61-79 EEE65047 hypothetical protein OsJ_20044 [Oryza 1.00 2806 siRNA sativa Japonica Group] 59123 NP_001056727 Os06g0136600 [Oryza sativa Japonica 0.97 2807 3082 Group] &gt; gi|55296986|dbj|BAD68461.1| putative enolase [Oryza sativa Japonica Group] &gt; gi|55297212|dbj|BAD68886.1| putative enolase [Oryza sativa Japonica Group] &gt; gi|113594767|dbj|BAF18641.1| Os06g0136600 [Oryza sativa Japonica Group] &gt; gi|218197519|gb|EEC79946.1| hypothetical protein OsI_21538 [Oryza sativa Indica Group] NP_001105896 enolase 1 [Zea mays] 0.92 2808 3083 &gt; gi|119355|sp|P26301.1|ENO1_MAIZE RecName: Full = Enolase 1; AltName: Full = 2-phospho-D-glycerate hydro-lyase 1; AltName: Full = 2-phosphoglycerate dehydratase 1 &gt; gi|22273|emb|CAA39454.1|enolase [Zea mays] ACL53816 unknown [Zea mays] 0.92 2809 3084 ACN26258 unknown [Zea mays] 0.88 2810 3085 ACG31732 enolase [Zea mays] 0.87 2811 3086 XP_002322420 predicted protein [Populus trichocarpa] 0.88 2812 3087 &gt; gi|118484871|gb|ABK94302.1| unknown [Populus trichocarpa] &gt; gi|222869416|gb|EEF06547.1| predicted protein [Populus trichocarpa] NP_001105371 enolase 2 [Zea mays] 0.87 2813 3088 &gt; gi|1169528|sp|P42895.1|ENO2_MAIZE RecName: Full = Enolase 2; AltName: Full = 2-phospho-D-glycerate hydro-lyase 2; AltName: Full = 2-phosphoglycerate dehydratase 2 &gt; gi|602253|gb|AAD04187.1|enolase [Zea mays] &gt; gi|37222051|gb|AAQ17040.2|pollen 2-phosphoglycerate dehydrogenase 2 precursor [Cynodon dactylon] ACF06525 enolase [Elaeis guineensis] 0.87 2814 3089 BAJ90134 predicted protein [Hordeum vulgare subsp. 0.87 2815 3090 vulgare] 45-63 NP_001143947 hypothetical protein LOC100276760 [Zea 1.00 2816 3091 mays] &gt; gi|195630673|gb|ACG36643.1| hypothetical protein [Zea mays] ACR36446 unknown [Zea mays] 0.97 2817 3092 NP_001143988 hypothetical protein LOC100276806 [Zea 0.79 2818 3093 mays] &gt; gi|194707932|gb|ACF88050.1| unknown [Zea mays] &gt; gi|195619872|gb|ACG31766.1| hypothetical protein [Zea mays] &gt; gi|195634969|gb|ACG36953.1| hypothetical protein [Zea mays] XP_002450179 hypothetical protein 0.81 2819 3094 SORBIDRAFT_05g001600 [Sorghum bicolor] &gt; gi|241936022|gb|EES09167.1| hypothetical protein SORBIDRAFT_05g001600 [Sorghum bicolor] NP_001065630 Os11g0127700 [Oryza sativa Japonica 0.70 2820 3095 Group] &gt; gi|77548485|gb|ABA91282.1| expressed protein [Oryza sativa Japonica Group] &gt; gi|113644334|dbj|BAF27475.1| Os11g0127700 [Oryza sativa Japonica Group] &gt; gi|125576047|gb|EAZ17269.1| hypothetical protein OsJ_32788 [Oryza sativa Japonica Group] EAY79782 hypothetical protein OsI_34938 [Oryza 0.70 2821 sativa Indica Group] 2433-2451 XP_002466271 hypothetical protein 1.00 2822 3096 SORBIDRAFT_01g004840 [Sorghum bicolor] &gt; gi|241920125|gb|EER93269.1| hypothetical protein SORBIDRAFT_01g004840 [Sorghum bicolor] NP_001145769 hypothetical protein LOC100279276 [Zea 0.98 2823 3097 mays] &gt; gi|219884363|gb|ACL52556.1| unknown [Zea mays] NP_001151633 vacuolar protein sorting 35 [Zea mays] 0.98 2824 3098 &gt; gi|195648240|gb|ACG43588.1| vacuolar protein sorting 35 [Zea mays] EAY92214 hypothetical protein OsI_13933 [Oryza 0.95 2825 sativa Indica Group] NP_001051593 Os03g0801600 [Oryza sativa Japonica 0.95 2826 3099 Group] &gt; gi|29150373|gb|AAO72382.1| putative vacuolar protein sorting- associated protein [Oryza sativa Japonica Group] &gt; gi|108711598|gb|ABF99393.1| vacuolar protein sorting-associated protein 35 family protein, putative, expressed [Oryza sativa Japonica Group] &gt; gi|113550064|dbj|BAF13507.1| Os03g0801600 [Oryza sativa Japonica Group] &gt; gi|125588278|gb|EAZ28942.1| hypothetical protein OsJ_12986 [Oryza sativa Japonica Group] &gt; gi|215704706|dbj|BAG94334.1| unnamed protein product [Oryza sativa Japonica Group] BAJ89592 predicted protein [Hordeum vulgare subsp. 0.95 2827 3100 vulgare] &gt; gi|326488479|dbj|BAJ93908.1| predicted protein [Hordeum vulgare subsp. vulgare] XP_002272227 PREDICTED: hypothetical protein isoform 0.82 2828 3101 1 [Vitis vinifera] XP_002272268 PREDICTED: hypothetical protein isoform 0.81 2829 3102 2 [Vitis vinifera] BAJ53108 JHL20J20.15 [Jatropha curcas] 0.79 2830 CBI17331 unnamed protein product [Vitis vinifera] 0.81 2831 20-38 XP_002442446 hypothetical protein 1.00 2832 3103 SORBIDRAFT_08g020140 [Sorghum bicolor] &gt; gi|241943139|gb|EES16284.1| hypothetical protein SORBIDRAFT_08g020140 [Sorghum bicolor] ACF80272 unknown [Zea mays] 0.84 2833 3104 NP_001141063 hypothetical protein LOC100273144 [Zea 0.84 2834 3105 mays] &gt; gi|194702462|gb|ACF85315.1| unknown [Zea mays] ACG44331 hypothetical protein [Zea mays] 0.84 2835 3106 BAJ89075 predicted protein [Hordeum vulgare subsp. 0.70 2836 3107 vulgare] &gt; gi|326533254|dbj|BAJ93599.1| predicted protein [Hordeum vulgare subsp. vulgare] Predicted 713-731 CAI30078 glycosyltransferase [Sorghum bicolor] 1.00 2837 3108 siRNA 57685 NP_001105165 glycosyltransferase [Zea mays] 0.96 2838 3109 &gt; gi|56409864|emb|CAI30080.1| glycosyltransferase [Zea mays] CAI30079 glycosyltransferase [Saccharum 0.95 2839 3110 officinarum] BAJ96053 predicted protein [Hordeum vulgare subsp. 0.81 2840 3111 vulgare] NP_001064930 Os10g0492200 [Oryza sativa Japonica 0.77 2841 3112 Group] &gt; gi|10140717|gb|AAG13551.1|AC023240_24 unknown protein [Oryza sativa Japonica Group] &gt; gi|31432816|gb|AAP54403.1| glycosyltransferase, putative, expressed [Oryza sativa Japonica Group] &gt; gi|113639539|dbj|BAF26844.1| Os10g0492200 [Oryza sativa Japonica Group] &gt; gi|215766456|dbj|BAG98764.1| unnamed protein product [Oryza sativa Japonica Group] &gt; gi|222613059|gb|EEE51191.1| hypothetical protein OsJ_31998 [Oryza sativa Japonica Group] EEC67226 hypothetical protein OsI_34143 [Oryza 0.76 2842 sativa Indica Group] CCA61105 TaGT61_1 [Triticum aestivum] 0.70 2843 3113 BAK04539 predicted protein [Hordeum vulgare subsp. 0.71 2844 3114 vulgare] NP_001046724 Os02g0331200 [Oryza sativa Japonica 0.71 2845 3115 Group] &gt; gi|46390170|dbj|BAD15603.1| putative HGA1 [Oryza sativa Japonica Group] &gt; gi|46390974|dbj|BAD16509.1| putative HGA1 [Oryza sativa Japonica Group] &gt; gi|113536255|dbj|BAF08638.1| Os02g0331200 [Oryza sativa Japonica Group] &gt; gi|215712340|dbj|BAG94467.1| unnamed protein product [Oryza sativa Japonica Group] &gt; gi|215737036|dbj|BAG95965.1| unnamed protein product [Oryza sativa Japonica Group] 344-362 YP_588428 hypothetical protein ZeamMp184 [Zea 1.00 2846 mays subsp. mays] &gt; gi|40795041|gb|AAR91085.1| hypothetical protein [Zea mays] &gt; gi|93116163|gb|ABE98794.1| hypothetical protein [Zea mays subsp. mays] &gt; gi|102579667|gb|ABF70947.1| hypothetical protein [Zea mays subsp. mays] ABE98707 hypothetical protein [Zea mays subsp. 0.90 2847 mays] 1201-1219 CAI30079 glycosyltransferase [Saccharum 1.00 2848 3116 officinarum] NP_001105165 glycosyltransferase [Zea mays] 0.96 2849 3117 &gt; gi|56409864|emb|CAI30080.1| glycosyltransferase [Zea mays] CAI30078 glycosyltransferase [Sorghum bicolor] 0.97 2850 3118 BAJ96053 predicted protein [Hordeum vulgare subsp. 0.83 2851 3119 vulgare] NP_001064930 Os10g0492200 [Oryza sativa Japonica 0.77 2852 3120 Group] &gt; gi|10140717|gb|AAG13551.1|AC023240_24 unknown protein [Oryza sativa Japonica Group] &gt; gi|31432816|gb|AAP54403.1| glycosyltransferase, putative, expressed [Oryza sativa Japonica Group] &gt; gi|113639539|dbj|BAF26844.1| Os10g0492200 [Oryza sativa Japonica Group] &gt; gi|215766456|dbj|BAG98764.1| unnamed protein product [Oryza sativa Japonica Group] &gt; gi|222613059|gb|EEE51191.1| hypothetical protein OsJ_31998 [Oryza sativa Japonica Group] EEC67226 hypothetical protein OsI_34143 [Oryza 0.78 2853 sativa Indica Group] BAK04539 predicted protein [Hordeum vulgare subsp. 0.71 2854 3121 vulgare] CCA61105 TaGT61_1 [Triticum aestivum] 0.70 2855 3122 BAD15602 putative HGA1 [Oryza sativa Japonica 0.71 2856 Group] &gt; gi|46390975|dbj|BAD16510.1| putative HGA1 [Oryza sativa Japonica Group] &gt; gi|215686668|dbj|BAG88921.1| unnamed protein product [Oryza sativa Japonica Group] NP_001046724 Os02g0331200 [Oryza sativa Japonica 0.71 2857 3123 Group] &gt; gi|46390170|dbj|BAD15603.1| putative HGA1 [Oryza sativa Japonica Group] &gt; gi|46390974|dbj|BAD16509.1| putative HGA1 [Oryza sativa Japonica Group] &gt; gi|113536255|dbj|BAF08638.1| Os02g0331200 [Oryza sativa Japonica Group] &gt; gi|215712340|dbj|BAG94467.1| unnamed protein product [Oryza sativa Japonica Group] &gt; gi|215737036|dbj|BAG95965.1| unnamed protein product [Oryza sativa Japonica Group] Predicted Jan- XP_002440494 hypothetical protein 1.00 2858 3124 siRNA 19 SORBIDRAFT_09g001910 [Sorghum 59993 bicolor] &gt; gi|241945779|gb|EES18924.1| hypothetical protein SORBIDRAFT_09g001910 [Sorghum bicolor] ACG27529 splicing factor, arginine/serine-rich 7 [Zea 0.87 2859 3125 mays] &gt; gi|223973611|gb|ACN30993.1| unknown [Zea mays] NP_001049771 Os03g0285900 [Oryza sativa Japonica 0.74 2860 3126 Group] &gt; gi|108707560|gb|ABF95355.1| RNA recognition motif family protein, expressed [Oryza sativa Japonica Group] &gt; gi|113548242|dbj|BAF11685.1| Os03g0285900 [Oryza sativa Japonica Group] &gt; gi|222624706|gb|EEE58838.1| hypothetical protein OsJ_10413 [Oryza sativa Japonica Group] EEC75006 hypothetical protein OsI_11073 [Oryza 0.70 2861 sativa Indica Group] NP_001132009 hypothetical protein LOC100193414 [Zea 0.72 2862 3127 mays] &gt; gi|194693184|gb|ACF80676.1| unknown [Zea mays] AAY84873 alternative splicing regulator [Triticum 0.72 2863 3128 aestivum] 14-32 XP_002458875 hypothetical protein 1.00 2864 3129 SORBIDRAFT_03g041990 [Sorghum bicolor] &gt; gi|241930850|gb|EES03995.1| hypothetical protein SORBIDRAFT_03g041990 [Sorghum bicolor] NP_001142991 hypothetical protein LOC100275452 [Zea 0.92 2865 3130 mays] &gt; gi|195612624|gb|ACG28142.1| hypothetical protein [Zea mays] NP_001145171 hypothetical protein LOC100278407 [Zea 0.91 2866 3131 mays] &gt; gi|195652179|gb|ACG45557.1| hypothetical protein [Zea mays] ACF83626 unknown [Zea mays] 0.92 2867 3132 EAZ14403 hypothetical protein OsJ_04323 [Oryza 0.89 2868 sativa Japonica Group] EAY76740 hypothetical protein OsI_04696 [Oryza 0.90 2869 sativa Indica Group] BAK00430 predicted protein [Hordeum vulgare subsp. 0.84 2870 3133 vulgare] 2352-2370 NP_001067313 Os12g0623900 [Oryza sativa Japonica 1.00 2871 3134 Group] &gt; gi|77556631|gb|ABA99427.1| 5-methyltetrahydropteroyltriglutamate- homocysteine methyltransferase, putative, expressed [Oryza sativa Japonica Group] &gt; gi|108862991|gb|ABG22094.1|5- methyltetrahydropteroyltriglutamate- homocysteine methyltransferase, putative, expressed [Oryza sativa Japonica Group] &gt; gi|113649820|dbj|BAF30332.1| Os12g0623900 [Oryza sativa Japonica Group] &gt; gi|125537469|gb|EAY83957.1| hypothetical protein OsI_39179 [Oryza sativa Indica Group] &gt; gi|125580128|gb|EAZ21274.1| hypothetical protein OsJ_36926 [Oryza sativa Japonica Group] ABG22095 5-methyltetrahydropteroyltriglutamate- 0.99 2872 homocysteine methyltransferase, putative, expressed [Oryza sativa Japonica Group] &gt; gi|108862993|gb|ABG22096.1|5- methyltetrahydropteroyltriglutamate- homocysteine methyltransferase, putative, expressed [Oryza sativa Japonica Group] NP_001067314 Os12g0624000 [Oryza sativa Japonica 0.98 2873 3135 Group] &gt; gi|77556632|gb|ABA99428.1| 5-methyltetrahydropteroyltriglutamate- homocysteine methyltransferase, putative, expressed [Oryza sativa Japonica Group] &gt; gi|77556633|gb|ABA99429.1|5- methyltetrahydropteroyltriglutamate- homocysteine methyltransferase, putative, expressed [Oryza sativa Japonica Group] &gt; gi|113649821|dbj|BAF30333.1| Os12g0624000 [Oryza sativa Japonica Group] &gt; gi|215695374|dbj|BAG90565.1| unnamed protein product [Oryza sativa Japonica Group] &gt; gi|218187282|gb|EEC69709.1| hypothetical protein OsI_39180 [Oryza sativa Indica Group] &gt; gi|222617511|gb|EEE53643.1| hypothetical protein OsJ_36927 [Oryza sativa Japonica Group] ACN28399 unknown [Zea mays] 0.95 2874 3136 AAL33589 methionine synthase [Zea mays] 0.94 2875 3137 ACL54117 unknown [Zea mays] 0.94 2876 3138 NP_001152513 LOC100286153 [Zea mays] 0.94 2877 3139 &gt; gi|195657041|gb|ACG47988.1|5- methyltetrahydropteroyltriglutamate-- homocysteine methyltransferase [Zea mays] CAJ01714 methionine synthase 2 enzyme [Hordeum 0.94 2878 3140 vulgare subsp. vulgare] &gt; gi|326512178|dbj|BAJ96070.1| predicted protein [Hordeum vulgare subsp. vulgare] BAJ89541 predicted protein [Hordeum vulgare subsp. 0.94 2879 3141 vulgare] BAJ88127 predicted protein [Hordeum vulgare subsp. 0.94 2880 3142 vulgare] 121-139 ACL52492 unknown [Zea mays] 1.00 2881 3143 XP_002465926 hypothetical protein 0.94 2882 3144 SORBIDRAFT_01g048350 [Sorghum bicolor] &gt; gi|241919780|gb|EER92924.1| hypothetical protein SORBIDRAFT_01g048350 [Sorghum bicolor] NP_001048855 Os03g0130500 [Oryza sativa Japonica 0.80 2883 3145 Group] &gt; gi|108706005|gb|ABF93800.1| EF hand family protein, expressed [Oryza sativa Japonica Group] &gt; gi|113547326|dbj|BAF10769.1| Os03g0130500 [Oryza sativa Japonica Group] EAZ25463 hypothetical protein OsJ_09285 [Oryza 0.79 2884 sativa Japonica Group] BAJ91192 predicted protein [Hordeum vulgare subsp. 0.80 2885 3146 vulgare] 49-67 ACV84253 LOX5 [Sorghum bicolor] 1.00 2886 3147 XP_002446554 hypothetical protein 1.00 2887 3148 SORBIDRAFT_06g018040 [Sorghum bicolor] &gt; gi|241937737|gb|EES10882.1| hypothetical protein SORBIDRAFT_06g018040 [Sorghum bicolor] ACL81190 tasselseed 1 [Zea mays] 0.95 2888 3149 NP_001105979 tassel seed1 [Zea mays] 0.95 2889 &gt; gi|84626293|gb|ABC59691.1| lipoxygenase [Zea mays] ACL81191 tasselseed 1b [Zea mays] 0.91 2890 3150 NP_001105978 lipoxygenase7 [Zea mays] 0.90 2891 3151 &gt; gi|84626291|gb|ABC59690.1| lipoxygenase [Zea mays] Q7XV13 RecName: Full = Putative lipoxygenase 5 0.86 2892 &gt; gi|38344820|emb|CAD40882.2| OSJNBa0064H22.1 [Oryza sativa Japonica Group] &gt; gi|116310177|emb|CAH67189.1| OSIGBa0152K17.1 [Oryza sativa Indica Group] BAJ94611 predicted protein [Hordeum vulgare subsp. 0.85 2893 3152 vulgare] &gt; gi|326511025|dbj|BAJ91860.1| predicted protein [Hordeum vulgare subsp. vulgare] CAI84707 lipoxygenase-like protein [Hordeum 0.85 2894 3153 vulgare subsp. vulgare] BAK03423 predicted protein [Hordeum vulgare subsp. 0.85 2895 3154 vulgare] 117-135 NP_001169757 hypothetical protein LOC100383639 [Zea 1.00 2896 3155 mays] &gt; gi|224031489|gb|ACN34820.1| unknown [Zea mays] &gt; gi|238006168|gb|ACR34119.1| unknown [Zea mays] ACG49031 ubiquinone biosynthesis methyltransferase 0.99 2897 3156 COQ5 [Zea mays] NP_001151844 ubiquinone biosynthesis methyltransferase 0.93 2898 3157 COQ5 [Zea mays] &gt; gi|195650199|gb|ACG44567.1| ubiquinone biosynthesis methyltransferase COQ5 [Zea mays] NP_001045568 Os01g0976600 [Oryza sativa Japonica 0.87 2899 3158 Group] &gt; gi|75320942|sp|Q5JNC0.1|COQ5_ORYSJ RecName: Full = 2-methoxy-6- polyprenyl-1,4-benzoquinol methylase, mitochondrial; AltName: Full = Ubiquinone biosynthesis methyltransferase COQ5; Flags: Precursor &gt; gi|57899522|dbj|BAD87036.1| putative ubiquinone [Oryza sativa Japonica Group] &gt; gi|113535099|dbj|BAF07482.1| Os01g0976600 [Oryza sativa Japonica Group] &gt; gi|215706368|dbj|BAG93224.1| unnamed protein product [Oryza sativa Japonica Group] &gt; gi|222619975|gb|EEE56107.1| hypothetical protein OsJ_04965 [Oryza sativa Japonica Group] EAY77437 hypothetical protein OsI_05431 [Oryza 0.87 2900 sativa Indica Group] BAJ89795 predicted protein [Hordeum vulgare subsp. 0.78 2901 3159 vulgare] XP_002457083 hypothetical protein 0.72 2902 3160 SORBIDRAFT_03g000990 [Sorghum bicolor] &gt; gi|241929058|gb|EES02203.1| hypothetical protein SORBIDRAFT_03g000990 [Sorghum bicolor] XP_002324457 predicted protein [Populus trichocarpa] 0.70 2903 3161 &gt; gi|222865891|gb|EEF03022.1| predicted protein [Populus trichocarpa] 656-674 XP_002444158 hypothetical protein 1.00 2904 3162 SORBIDRAFT_07g010240 [Sorghum bicolor] &gt; gi|241940508|gb|EES13653.1| hypothetical protein SORBIDRAFT_07g010240 [Sorghum bicolor] NP_001168336 hypothetical protein LOC100382104 [Zea 0.85 2905 3163 mays] &gt; gi|223947549|gb|ACN27858.1| unknown [Zea mays] EAY97519 hypothetical protein OsI_19447 [Oryza 0.80 2906 sativa Indica Group] &gt; gi|222631101|gb|EEE63233.1| hypothetical protein OsJ_18043 [Oryza sativa Japonica Group] BAJ96779 predicted protein [Hordeum vulgare subsp. 0.78 2907 3164 vulgare] BAK02037 predicted protein [Hordeum vulgare subsp. 0.78 2908 3165 vulgare] BAJ97459 predicted protein [Hordeum vulgare subsp. 0.78 2909 3166 vulgare] AAV44115 unknown protein [Oryza sativa Japonica 0.78 2910 Group] 668-686 NP_001150708 zinc finger, C2H2 type family protein [Zea 1.00 2911 3167 mays] &gt; gi|195641252|gb|ACG40094.1| zinc finger, C2H2 type family protein [Zea mays] XP_002460014 hypothetical protein 0.87 2912 3168 SORBIDRAFT_02g020840 [Sorghum bicolor] &gt; gi|241923391|gb|EER96535.1| hypothetical protein SORBIDRAFT_02g020840 [Sorghum bicolor] NP_001150040 zinc finger, C2H2 type family protein [Zea 0.78 2913 3169 mays] &gt; gi|195636264|gb|ACG37600.1| zinc finger, C2H2 type family protein [Zea mays] BAK04496 predicted protein [Hordeum vulgare subsp. 0.70 2914 3170 vulgare] 524-542 NP_001183648 hypothetical protein LOC100502242 [Zea 1.00 2915 3171 mays] &gt; gi|238013654|gb|ACR37862.1| unknown [Zea mays] 660-678 NP_001150040 zinc finger, C2H2 type family protein [Zea 1.00 2916 3172 mays] &gt; gi|195636264|gb|ACG37600.1| zinc finger, C2H2 type family protein [Zea mays] XP_002460014 hypothetical protein 0.78 2917 3173 SORBIDRAFT_02g020840 [Sorghum bicolor] &gt; gi|241923391|gb|EER96535.1| hypothetical protein SORBIDRAFT_02g020840 [Sorghum bicolor] NP_001150708 zinc finger, C2H2 type family protein [Zea 0.76 2918 3174 mays] &gt; gi|195641252|gb|ACG40094.1| zinc finger, C2H2 type family protein [Zea mays] 433-451 NP_001140599 hypothetical protein LOC100272670 [Zea 1.00 2919 3175 mays] &gt; gi|194700138|gb|ACF84153.1| unknown [Zea mays] 277-295 XP_002468199 hypothetical protein 1.00 2920 3176 SORBIDRAFT_01g041550 [Sorghum bicolor] &gt; gi|241922053|gb|EER95197.1| hypothetical protein SORBIDRAFT_01g041550 [Sorghum bicolor] NP_001140579 hypothetical protein LOC100272649 [Zea 0.88 2921 3177 mays] &gt; gi|194700056|gb|ACF84112.1| unknown [Zea mays] ACG35337 purple acid phosphatase 1 [Zea mays] 0.84 2922 3178 NP_001049500 Os03g0238600 [Oryza sativa Japonica 0.78 2923 3179 Group] &gt; gi|108707073|gb|ABF94868.1| purple acid phosphatase 1, putative, expressed [Oryza sativa Japonica Group] &gt; gi|113547971|dbj|BAF11414.1| Os03g0238600 [Oryza sativa Japonica Group] &gt; gi|125543049|gb|EAY89188.1| hypothetical protein OsI_10684 [Oryza sativa Indica Group] &gt; gi|125585546|gb|EAZ26210.1| hypothetical protein OsJ_10077 [Oryza sativa Japonica Group] &gt; gi|215697901|dbj|BAG92094.1| unnamed protein product [Oryza sativa Japonica Group] &gt; gi|215737711|dbj|BAG96841.1| unnamed protein product [Oryza sativa Japonica Group] &gt; gi|215740770|dbj|BAG96926.1| unnamed protein product [Oryza sativa Japonica Group] &gt; gi|215767489|dbj|BAG99717.1| unnamed protein product [Oryza sativa Japonica Group] NP_001151094 LOC100284727 [Zea mays] 0.75 2924 3180 &gt; gi|195644254|gb|ACG41595.1|purple acid phosphatase 1 [Zea mays] ACF83217 unknown [Zea mays] 0.74 2925 3181 ACN25848 unknown [Zea mays] 0.74 2926 3182 ACL54336 unknown [Zea mays] 0.74 2927 3183 BAI78301 purple acid phosphatase [Triticum 0.72 2928 3184 aestivum] BAK06221 predicted protein [Hordeum vulgare subsp. 0.73 2929 3185 vulgare] &gt; gi|326500986|dbj|BAJ98724.1| predicted protein [Hordeum vulgare subsp. vulgare] &gt; gi|326530618|dbj|BAK01107.1| predicted protein [Hordeum vulgare subsp. vulgare] 121-139 XP_002465926 hypothetical protein 1.00 2930 3186 SORBIDRAFT_01g048350 [Sorghum bicolor] &gt; gi|241919780|gb|EER92924.1| hypothetical protein SORBIDRAFT_01g048350 [Sorghum bicolor] ACL52492 unknown [Zea mays] 0.94 2931 3187 NP_001048855 Os03g0130500 [Oryza sativa Japonica 0.82 2932 3188 Group] &gt; gi|108706005|gb|ABF93800.1| EF hand family protein, expressed [Oryza sativa Japonica Group] &gt; gi|113547326|dbj|BAF10769.1| Os03g0130500 [Oryza sativa Japonica Group] EAZ25463 hypothetical protein OsJ_09285 [Oryza 0.80 2933 sativa Japonica Group] BAJ91192 predicted protein [Hordeum vulgare subsp. 0.81 2934 3189 vulgare] 2507-2525 XP_002454851 hypothetical protein 1.00 2935 3190 SORBIDRAFT_04g038540 [Sorghum bicolor] &gt; gi|241934682|gb|EES07827.1| hypothetical protein SORBIDRAFT_04g038540 [Sorghum bicolor] NP_001183894 hypothetical protein LOC100502487 [Zea 0.90 2936 3191 mays] &gt; gi|238015298|gb|ACR38684.1| unknown [Zea mays] BAJ86117 predicted protein [Hordeum vulgare subsp. 0.76 2937 3192 vulgare] &gt; gi|326521078|dbj|BAJ96742.1| predicted protein [Hordeum vulgare subsp. vulgare] NP_001050813 Os03g0657100 [Oryza sativa Japonica 0.70 2938 3193 Group] &gt; gi|108710182|gb|ABF97977.1| U-box domain containing protein, expressed [Oryza sativa Japonica Group] &gt; gi|113549284|dbj|BAF12727.1| Os03g0657100 [Oryza sativa Japonica Group] &gt; gi|218193430|gb|EEC75857.1| hypothetical protein OsI_12864 [Oryza sativa Indica Group] AAP50990 unknown protein [Oryza sativa Japonica 0.70 2939 3194 Group] 746-764 NP_001140297 hypothetical protein LOC100272342 [Zea 1.00 2940 3195 mays] &gt; gi|194698890|gb|ACF83529.1| unknown [Zea mays] 49-67 NP_001105978 lipoxygenase7 [Zea mays] 1.00 2941 3196 &gt; gi|84626291|gb|ABC59690.1| lipoxygenase [Zea mays] ACL81191 tasselseed 1b [Zea mays] 0.98 2942 3197 XP_002446554 hypothetical protein 0.91 2943 3198 SORBIDRAFT_06g018040 [Sorghum bicolor] &gt; gi|241937737|gb|EES10882.1| hypothetical protein SORBIDRAFT_06g018040 [Sorghum bicolor] ACV84253 LOX5 [Sorghum bicolor] 0.90 2944 3199 NP_001105979 tassel seed1 [Zea mays] 0.91 2945 3200 &gt; gi|84626293|gb|ABC59691.1| lipoxygenase [Zea mays] ACL81190 tasselseed 1 [Zea mays] 0.90 2946 3201 Q7XV13 RecName: Full = Putative lipoxygenase 5 0.83 2947 &gt; gi|38344820|emb|CAD40882.2| OSJNBa0064H22.1 [Oryza sativa Japonica Group] &gt; gi|116310177|emb|CAH67189.1| OSIGBa0152K17.1 [Oryza sativa Indica Group] CAI84707 lipoxygenase-like protein [Hordeum 0.82 2948 3202 vulgare subsp. vulgare] BAJ94611 predicted protein [Hordeum vulgare subsp. 0.82 2949 3203 vulgare] &gt; gi|326511025|dbj|BAJ91860.1| predicted protein [Hordeum vulgare subsp. vulgare] BAK03423 predicted protein [Hordeum vulgare subsp. 0.82 2950 3204 vulgare] Predicted 256-274 XP_002461947 hypothetical protein 1.00 2951 3205 siRNA SORBIDRAFT_02g011010 [Sorghum 60012 bicolor] &gt; gi|241925324|gb|EER98468.1| hypothetical protein SORBIDRAFT_02g011010 [Sorghum bicolor] 948-966 XP_002457764 hypothetical protein 1.00 2952 3206 SORBIDRAFT_03g013140 [Sorghum bicolor] &gt; gi|241929739|gb|EES02884.1| hypothetical protein SORBIDRAFT_03g013140 [Sorghum bicolor] ACF87582 unknown [Zea mays] 0.93 2953 3207 NP_001105753 Pti1 protein [Zea mays] 0.92 2954 3208 &gt; gi|49188602|gb|AAT57904.1|putative PTI1-like kinase [Zea mays] AAT57905 putative PTI1-like kinase [Zea mays] 0.92 2955 3209 AAT57906 putative PTI1-like kinase [Zea mays] 0.93 2956 3210 EAY73772 hypothetical protein OsI_01646 [Oryza 0.81 2957 sativa Indica Group] NP_001042895 Os01g0323100 [Oryza sativa Japonica 0.81 2958 3211 Group] &gt; gi|12328582|dbj|BAB21241.1| putative Pto kinase interactor 1 [Oryza sativa Japonica Group] &gt; gi|29027802|dbj|BAC65877.1| putative Pto kinase interactor 1 [Oryza sativa Japonica Group] &gt; gi|113532426|dbj|BAF04809.1| Os01g0323100 [Oryza sativa Japonica Group] &gt; gi|125570160|gb|EAZ11675.1| hypothetical protein OsJ_01536 [Oryza sativa Japonica Group] &gt; gi|215694472|dbj|BAG89427.1| unnamed protein product [Oryza sativa Japonica Group] 1207-1225 XP_002451738 hypothetical protein 1.00 2959 3212 SORBIDRAFT_04g006950 [Sorghum bicolor] &gt; gi|241931569|gb|EES04714.1| hypothetical protein SORBIDRAFT_04g006950 [Sorghum bicolor] ACG38564 cerebral protein 1 [Zea mays] 0.96 2960 3213 &gt; gi|238015424|gb|ACR38747.1| unknown [Zea mays] NP_001047303 Os02g0593900 [Oryza sativa Japonica 0.70 2961 3214 Group] &gt; gi|113536834|dbj|BAF09217.1| Os02g0593900 [Oryza sativa Japonica Group] &gt; gi|125540112|gb|EAY86507.1| hypothetical protein OsI_07887 [Oryza sativa Indica Group] &gt; gi|125582717|gb|EAZ23648.1| hypothetical protein OsJ_07349 [Oryza sativa Japonica Group] 67-85 XP_002449762 hypothetical protein 1.00 2962 3215 SORBIDRAFT_05g022780 [Sorghum bicolor] &gt; gi|241935605|gb|EES08750.1| hypothetical protein SORBIDRAFT_05g022780 [Sorghum bicolor] ABA94447 ETO1-like protein 1, putative, expressed 0.93 2963 3216 [Oryza sativa Japonica Group] &gt; gi|125577639|gb|EAZ18861.1| hypothetical protein OsJ_34400 [Oryza sativa Japonica Group] EAY81449 hypothetical protein OsI_36620 [Oryza 0.93 2964 sativa Indica Group] NP_001068166 Os11g0585900 [Oryza sativa Japonica 0.90 2965 3217 Group] &gt; gi|113645388|dbj|BAF28529.1| Os11g0585900 [Oryza sativa Japonica Group] NP_001146335 hypothetical protein LOC100279911 [Zea 0.91 2966 3218 mays] &gt; gi|219886675|gb|ACL53712.1| unknown [Zea mays] CBI25039 unnamed protein product [Vitis vinifera] 0.73 2967 XP_002280519 PREDICTED: hypothetical protein [Vitis 0.72 2968 3219 vinifera] AAZ08351 ethylene overproducer-like 1 [Solanum 0.71 2969 3220 lycopersicum] ABB46489 ethylene-overproducer1-like protein 0.71 2970 3221 [Solanum lycopersicum] Predicted 358-381 XP_002466013 hypothetical protein 1.00 2971 3222 siRNA SORBIDRAFT_01g050070 [Sorghum 55081 bicolor] &gt; gi|241919867|gb|EER93011.1| hypothetical protein SORBIDRAFT_01g050070 [Sorghum bicolor] NP_001132709 hypothetical protein LOC100194192 [Zea 0.77 2972 3223 mays] &gt; gi|194695168|gb|ACF81668.1| unknown [Zea mays] NP_001148998 xyloglucan endotransglucosylase/hydrolase 0.76 2973 3224 protein 32 [Zea mays] &gt; gi|195623856|gb|ACG33758.1| xyloglucan endotransglucosylase/hydrolase protein 32 precursor [Zea mays] Predicted 364-382 XP_002439498 hypothetical protein 1.00 2974 3225 siRNA SORBIDRAFT_09g008130 [Sorghum 59032 bicolor] &gt; gi|241944783|gb|EES17928.1| hypothetical protein SORBIDRAFT_09g008130 [Sorghum bicolor] NP_001141460 hypothetical protein LOC100273570 [Zea 0.88 2975 3226 mays] &gt; gi|194704658|gb|ACF86413.1| unknown [Zea mays] &gt; gi|195620084|gb|ACG31872.1|triose phosphate/phosphate translocator [Zea mays] &gt; gi|195625418|gb|ACG34539.1| triose phosphate/phosphate translocator [Zea mays] ACG32515 triose phosphate/phosphate translocator 0.88 2976 3227 [Zea mays] NP_001055001 Os05g0241200 [Oryza sativa Japonica 0.75 2977 3228 Group] &gt; gi|53980843|gb|AAV24764.1| putative phosphate translocator [Oryza sativa Japonica Group] &gt; gi|113578552|dbj|BAF16915.1| Os05g0241200 [Oryza sativa Japonica Group] &gt; gi|125551487|gb|EAY97196.1| hypothetical protein OsI_19118 [Oryza sativa Indica Group] &gt; gi|215765430|dbj|BAG87127.1| unnamed protein product [Oryza sativa Japonica Group] BAJ94007 predicted protein [Hordeum vulgare subsp. 0.73 2978 3229 vulgare] 225-243 NP_001168807 hypothetical protein LOC100382608 [Zea 1.00 2979 3230 mays] &gt; gi|223973113|gb|ACN30744.1| unknown [Zea mays] Predicted 227-245 XP_002447337 hypothetical protein 1.00 2980 3231 siRNA SORBIDRAFT_06g033160 [Sorghum 58721 bicolor] &gt; gi|241938520|gb|EES11665.1| hypothetical protein SORBIDRAFT_06g033160 [Sorghum bicolor] NP_001142056 hypothetical protein LOC100274212 [Zea 0.96 2981 3232 mays] &gt; gi|194706940|gb|ACF87554.1| unknown [Zea mays] &gt; gi|223947485|gb|ACN27826.1| unknown [Zea mays] ACG45259 hypothetical protein [Zea mays] 0.96 2982 3233 EEC78262 hypothetical protein OsI_17948 [Oryza 0.86 2983 sativa Indica Group] EEE61915 hypothetical protein OsJ_16648 [Oryza 0.86 2984 sativa Japonica Group] BAJ96591 predicted protein [Hordeum vulgare subsp. 0.86 2985 3234 vulgare] CAJ86266 H0901F07.3 [Oryza sativa Indica Group] 0.76 2986 3235 614-632 NP_001183362 hypothetical protein LOC100501771 [Zea 1.00 2987 3236 mays] &gt; gi|238011008|gb|ACR36539.1| unknown [Zea mays] XP_002453714 hypothetical protein 0.92 2988 3237 SORBIDRAFT_04g011130 [Sorghum bicolor] &gt; gi|241933545|gb|EES06690.1| hypothetical protein SORBIDRAFT_04g011130 [Sorghum bicolor] BAJ91736 predicted protein [Hordeum vulgare subsp. 0.80 2989 3238 vulgare] NP_001043916 Os01g0687800 [Oryza sativa Japonica 0.76 2990 3239 Group] &gt; gi|18844891|dbj|BAB85360.1| FAD binding domain containing protein- like [Oryza sativa Japonica Group] &gt; gi|113533447|dbj|BAF05830.1| Os01g0687800 [Oryza sativa Japonica Group] EAY75418 hypothetical protein OsI_03321 [Oryza 0.75 2991 sativa Indica Group] 361-379 XP_002441149 hypothetical protein 1.00 2992 3240 SORBIDRAFT_09g021260 [Sorghum bicolor] &gt; gi|241946434|gb|EES19579.1| hypothetical protein SORBIDRAFT_09g021260 [Sorghum bicolor] NP_001152458 phosphoribosylanthranilate transferase 0.97 2993 3241 [Zea mays] &gt; gi|195656517|gb|ACG47726.1| phosphoribosylanthranilate transferase [Zea mays] BAK04138 predicted protein [Hordeum vulgare subsp. 0.89 2994 3242 vulgare] EEC79273 hypothetical protein OsI_20060 [Oryza 0.90 2995 sativa Indica Group] NP_001055620 Os05g0429700 [Oryza sativa Japonica 0.90 2996 3243 Group] &gt; gi|55733914|gb|AAV59421.1| putative anthranilate phosphoribosyltransferase [Oryza sativa Japonica Group] &gt; gi|113579171|dbj|BAF17534.1| Os05g0429700 [Oryza sativa Japonica Group] &gt; gi|215737213|dbj|BAG96142.1| unnamed protein product [Oryza sativa Japonica Group] &gt; gi|222631675|gb|EEE63807.1| hypothetical protein OsJ_18631 [Oryza sativa Japonica Group] 149-167 XP_002454239 hypothetical protein 1.00 2997 3244 SORBIDRAFT_04g027330 [Sorghum bicolor] &gt; gi|241934070|gb|EES07215.1| hypothetical protein SORBIDRAFT_04g027330 [Sorghum bicolor] ACG32582 heat shock 22 kDa protein [Zea mays] 0.88 2998 3245 NP_001105607 low molecular weight heat shock protein 0.88 2999 3246 precursor [Zea mays] &gt; gi|3015621|gb|AAC12279.1|low molecular weight heat shock protein precursor [Zea mays] &gt; gi|54299342|gb|AAV32521.1| mitochondrial small heat shock protein 22 [Zea mays] &gt; gi|195622718|gb|ACG33189.1|heat shock 22 kDa protein [Zea mays] ACF84470 unknown [Zea mays] 0.87 3000 3247 NP_01048175 Os02g0758000 [Oryza sativa Japonica 0.80 3001 3248 Group] &gt; gi|75294195|sp|Q6Z7V2.1|HS24M_ORYSJ RecName: Full = 24.1 kDa heat shock protein, mitochondrial; Short = OsHsp24.1; Flags: Precursor &gt; gi|46805691|dbj|BAD17092.1| putative low molecular weight heat shock protein [Oryza sativa Japonica Group] &gt; gi|113537706|dbj|BAF10089.1| Os02g0758000 [Oryza sativa Japonica Group] &gt; gi|125541201|gb|EAY87596.1| hypothetical protein OsI_09007 [Oryza sativa Indica Group] &gt; gi|125583753|gb|EAZ24684.1| hypothetical protein OsJ_08454 [Oryza sativa Japonica Group] &gt; gi|215693891|dbj|BAG89090.1| unnamed protein product [Oryza sativa Japonica Group] &gt; gi|215704321|dbj|BAG93755.1| unnamed protein product [Oryza sativa Japonica Group] &gt; gi|215740605|dbj|BAG97261.1| unnamed protein product [Oryza sativa Japonica Group] &gt; gi|313575793|gb|ADR66976.1|22 kDa heat shock protein [Oryza sativa Japonica Group] &gt; gi|332691639|gb|AEE90022.1| mitochondrial small heat shock protein [Oryza sativa Japonica Group] 270-288 XP_02444207 hypothetical protein 1.00 3002 3249 SORBIDRAFT_07g014990 [Sorghum bicolor] &gt; gi|241940557|gb|EES13702.1| hypothetical protein SORBIDRAFT_07g014990 [Sorghum bicolor] NP_001145845 hypothetical protein LOC100279356 [Zea 0.91 3003 3250 mays] &gt; gi|219884681|gb|ACL52715.1| unknown [Zea mays] NP_001061645 Os08g0365900 [Oryza sativa Japonica 0.77 3004 3251 Group] &gt; gi|38636850|dbj|BAD03090.1| putative nucleolar protein [Oryza sativa Japonica Group] &gt; gi|113623614|dbj|BAF23559.1| Os08g0365900 [Oryza sativa Japonica Group] &gt; gi|215717005|dbj|BAG95368.1| unnamed protein product [Oryza sativa Japonica Group] &gt; gi|222640447|gb|EEE68579.1| hypothetical protein OsJ_27075 [Oryza sativa Japonica Group] 436-454 Redundant master homologue-skipping 40959 NP_001147431 aconitase2 [Zea mays] 1.00 3005 3252 &gt; gi|195611330|gb|ACG27495.1| aconitate hydratase, cytoplasmic [Zea mays] &gt; gi|223948253|gb|ACN28210.1| unknown [Zea mays] XP_002445174 hypothetical protein 0.78 3006 3253 SORBIDRAFT_07g005390 [Sorghum bicolor] &gt; gi|241941524|gb|EES14669.1| hypothetical protein SORBIDRAFT_07g005390 [Sorghum bicolor] Q6YZX6 RecName: Full = Putative aconitate 0.77 3007 hydratase, cytoplasmic; Short = Aconitase; AltName: Full = Citrate hydro-lyase &gt; gi|40253814|dbj|BAD05751.1| putative Aconitate hydratase [Oryza sativa Japonica Group] BAJ85661 predicted protein [Hordeum vulgare subsp. 0.76 3008 3254 vulgare] CBE71057 aconitate hydratase 3 [Citrus clementina] 0.75 3009 3255 ADZ57218 aconitase protein [Litchi chinensis] 0.74 3010 3256 XP_002524184 aconitase, putative [Ricinus communis] 0.74 3011 3257 &gt; gi|223536553|gb|EEF38199.1| aconitase, putative [Ricinus communis] XP_002278138 PREDICTED: hypothetical protein [Vitis 0.74 3012 3258 vinifera] &gt; gi|297737441|emb|CBI26642.3| unnamed protein product [Vitis vinifera] P49608 RecName: Full = Aconitate hydratase, 0.75 3013 cytoplasmic; Short = Aconitase; AltName: Full = Citrate hydro-lyase &gt; gi|7437043|pir||T10101 aconitate hydratase (EC 4.2.1.3) - cucurbit &gt; gi|868003|dbj|BAA06108.1|aconitase [Cucurbita cv. Kurokawa Amakuri] CBE71058 aconitate hydratase 2 [Citrus clementina] 0.74 3014 3259 529-547 XP_002453714 hypothetical protein 1.00 3015 3260 SORBIDRAFT_04g011130 [Sorghum bicolor] &gt; gi|241933545|gb|EES06690.1| hypothetical protein SORBIDRAFT_04g011130 [Sorghum bicolor] NP_001183362 hypothetical protein LOC100501771 [Zea 0.85 3016 3261 mays] &gt; gi|238011008|gb|ACR36539.1| unknown [Zea mays] BAJ91736 predicted protein [Hordeum vulgare subsp. 0.80 3017 3262 vulgare] NP_001043916 Os01g0687800 [Oryza sativa Japonica 0.76 3018 3263 Group] &gt; gi|18844891|dbj|BAB85360.1| FAD binding domain containing protein- like [Oryza sativa Japonica Group] &gt; gi|113533447|dbj|BAF05830.1| Os01g0687800 [Oryza sativa Japonica Group] EAY75418 hypothetical protein OsI_03321 [Oryza 0.75 3019 sativa Indica Group] Predicted 254-273 XP_002489117 hypothetical protein 1.00 3020 3264 siRNA SORBIDRAFT_0057s002150 [Sorghum 58877 bicolor] &gt; gi|241947368|gb|EES20513.1| hypothetical protein SORBIDRAFT_0057s002150 [Sorghum bicolor] XP_002488963 hypothetical protein 0.94 3021 3265 SORBIDRAFT_1150s002010 [Sorghum bicolor] &gt; gi|241946997|gb|EES20142.1| hypothetical protein SORBIDRAFT_1150s002010 [Sorghum bicolor] XP_002464695 hypothetical protein 0.84 3022 3266 SORBIDRAFT_01g023641 [Sorghum bicolor] &gt; gi|241918549|gb|EER91693.1| hypothetical protein SORBIDRAFT_01g023641 [Sorghum bicolor] XP_002450731 hypothetical protein 0.80 3023 3267 SORBIDRAFT_05g016471 [Sorghum bicolor] &gt; gi|241936574|gb|EES09719.1| hypothetical protein SORBIDRAFT_05g016471 [Sorghum bicolor] XP_002450732 hypothetical protein 0.79 3024 3268 SORBIDRAFT_05g016475 [Sorghum bicolor] &gt; gi|241936575|gb|EES09720.1| hypothetical protein SORBIDRAFT_05g016475 [Sorghum bicolor] Predicted 632-655 XP_002447941 hypothetical protein 1.00 3025 3269 siRNA SORBIDRAFT_06g018520 [Sorghum 57179 bicolor] &gt; gi|241939124|gb|EES12269.1| hypothetical protein SORBIDRAFT_06g018520 [Sorghum bicolor] NP_001159183 hypothetical protein LOC100304268 [Zea 0.99 3026 3270 mays] &gt; gi|195627604|gb|ACG35632.1| ubiquitin carboxyl-terminal hydrolase 4 [Zea mays] &gt; gi|223942511|gb|ACN25339.1| unknown [Zea mays] NP_001146737 hypothetical protein LOC100280339 [Zea 0.99 3027 3271 mays] &gt; gi|219888545|gb|ACL54647.1| unknown [Zea mays] &gt; gi|223974199|gb|ACN31287.1| unknown [Zea mays] ACG34260 ubiquitin carboxyl-terminal hydrolase 4 0.99 3028 3272 [Zea mays] NP_001052951 Os04g0452400 [Oryza sativa Japonica 0.97 3029 3273 Group] &gt; gi|113564522|dbj|BAF14865.1| Os04g0452400 [Oryza sativa Japonica Group] &gt; gi|215695550|dbj|BAG90741.1| unnamed protein product [Oryza sativa Japonica Group] BAK02826 predicted protein [Hordeum vulgare subsp. 0.96 3030 3274 vulgare] CAD40853 OSJNBa0086B14.26 [Oryza sativa 0.97 3031 Japonica Group] &gt; gi|116310175|emb|CAH67188.1| H0815C01.9 [Oryza sativa Indica Group] &gt; gi|125548525|gb|EAY94347.1| hypothetical protein OsI_16114 [Oryza sativa Indica Group] &gt; gi|125590577|gb|EAZ30927.1| hypothetical protein OsJ_15007 [Oryza sativa Japonica Group] XP_002270407 PREDICTED: hypothetical protein [Vitis 0.87 3032 3275 vinifera] XP_002531269 Ubiquitin carboxyl-terminal hydrolase, 0.88 3033 3276 putative [Ricinus communis] &gt; gi|223529154|gb|EEF31133.1| Ubiquitin carboxyl-terminal hydrolase, putative [Ricinus communis] CAN67091 hypothetical protein VITISV_006756 0.86 3034 [Vitis vinifera] Predicted 490-510 XP_002446760 hypothetical protein 1.00 3035 3277 siRNA SORBIDRAFT_06g021990 [Sorghum 55393 bicolor] &gt; gi|241937943|gb|EES11088.1| hypothetical protein SORBIDRAFT_06g021990 [Sorghum bicolor] ACG37655 anther-specific proline-rich protein APG 0.86 3036 3278 [Zea mays] ACG35356 anther-specific proline-rich protein APG 0.86 3037 3279 [Zea mays] NP_001132075 hypothetical protein LOC100193489 [Zea 0.86 3038 3280 mays] &gt; gi|194693356|gb|ACF80762.1| unknown [Zea mays] NP_001141295 hypothetical protein LOC100273386 [Zea 0.87 3039 3281 mays] &gt; gi|194703842|gb|ACF86005.1| unknown [Zea mays] NP_001053264 Os04g0507700 [Oryza sativa Japonica 0.75 3040 3282 Group] &gt; gi|32489520|emb|CAE04723.1| OSJNBa0043L24.11 [Oryza sativa Japonica Group] &gt; gi|38567850|emb|CAE05693.2| OSJNBb0002J11.20 [Oryza sativa Japonica Group] &gt; gi|113564835|dbj|BAF15178.1| Os04g0507700 [Oryza sativa Japonica Group] &gt; gi|116310323|emb|CAH67339.1| OSIGBa0157A06.8 [Oryza sativa Indica Group] &gt; gi|116310765|emb|CAH67558.1| OSIGBa0101P20.1 [Oryza sativa Indica Group] &gt; gi|125548968|gb|EAY94790.1| hypothetical protein OsI_16569 [Oryza sativa Indica Group] &gt; gi|125590941|gb|EAZ31291.1| hypothetical protein OsJ_15397 [Oryza sativa Japonica Group] BAJ85303 predicted protein [Hordeum vulgare subsp. 0.73 3041 3283 vulgare] 66-86 NP_001104926 MADS2 [Zea mays] 1.00 3042 3284 &gt; gi|29611976|gb|AAO85643.1|MADS- box transcription factor MADS2 [Zea mays] AAG09919 MADS box protein 2 [Zea mays] 1.00 3043 3285 NP_001047230 Os02g0579600 [Oryza sativa Japonica 0.86 3044 3286 Group] &gt; gi|91207149|sp|Q6EP49.2|MAD27_ORYSJ RecName: Full = MADS-box transcription factor 27; AltName: Full = OsMADS27; AltName: Full = RMADS218 &gt; gi|30313677|gb|AAO47706.1| transcription factor MADS27 [Oryza sativa Japonica Group] &gt; gi|113536761|dbj|BAF09144.1| Os02g0579600 [Oryza sativa Japonica Group] EEC73475 hypothetical protein OsI_07801 [Oryza 0.84 3045 sativa Indica Group] &gt; gi|222623119|gb|EEE57251.1| hypothetical protein OsJ_07263 [Oryza sativa Japonica Group] BAK01931 predicted protein [Hordeum vulgare subsp. 0.79 3046 3287 vulgare] CAM59078 MIKC-type MADS-box transcription 0.79 3047 3288 factor WM30 [Triticum aestivum] CAD40988 OSJNBa0072F16.13 [Oryza sativa 0.77 3048 Japonica Group] BAD29571 putative transcription factor MADS27 0.76 3049 [Oryza sativa Japonica Group] 571-591 NP_001047230 Os02g0579600 [Oryza sativa Japonica 1.00 3050 3289 Group] &gt; gi|91207149|sp|Q6EP49.2|MAD27_ORYSJ RecName: Full = MADS-box transcription factor 27; AltName: Full = OsMADS27; AltName: Full = RMADS218 &gt; gi|30313677|gb|AAO47706.1| transcription factor MADS27 [Oryza sativa Japonica Group] &gt; gi|113536761|dbj|BAF09144.1| Os02g0579600 [Oryza sativa Japonica Group] EEC73475 hypothetical protein OsI_07801 [Oryza 0.98 3051 sativa Indica Group] &gt; gi|222623119|gb|EEE57251.1| hypothetical protein OsJ_07263 [Oryza sativa Japonica Group] BAD29571 putative transcription factor MADS27 0.90 3052 [Oryza sativa Japonica Group] AAG09919 MADS box protein 2 [Zea mays] 0.86 3053 3290 NP_001104926 MADS2 [Zea mays] 0.86 3054 3291 &gt; gi|29611976|gb|AAO85643.1|MADS- box transcription factor MADS2 [Zea mays] BAK01931 predicted protein [Hordeum vulgare subsp. 0.82 3055 3292 vulgare] CAD40988 OSJNBa0072F16.13 [Oryza sativa 0.80 3056 Japonica Group] CAM59078 MIKC-type MADS-box transcription 0.81 3057 3293 factor WM30 [Triticum aestivum] 481-501 NP_001132075 hypothetical protein LOC100193489 [Zea 1.00 3058 3294 mays] &gt; gi|194693356|gb|ACF80762.1| unknown [Zea mays] ACG35356 anther-specific proline-rich protein APG 1.00 3059 3295 [Zea mays] ACG37655 anther-specific proline-rich protein APG 0.92 3060 3296 [Zea mays] NP_001141295 hypothetical protein LOC100273386 [Zea 0.92 3061 3297 mays] &gt; gi|194703842|gb|ACF86005.1| unknown [Zea mays] XP_002446760 hypothetical protein 0.91 3062 3298 SORBIDRAFT_06g021990 [Sorghum bicolor] &gt; gi|241937943|gb|EES11088.1| hypothetical protein SORBIDRAFT_06g021990 [Sorghum bicolor] NP_001053264 Os04g0507700 [Oryza sativa Japonica 0.81 3063 3299 Group] &gt; gi|32489520|emb|CAE04723.1| OSJNBa0043L24.11 [Oryza sativa Japonica Group] &gt; gi|38567850|emb|CAE05693.2| OSJNBb0002J11.20 [Oryza sativa Japonica Group] &gt; gi|113564835|dbj|BAF15178.1| Os04g0507700 [Oryza sativa Japonica Group] &gt; gi|116310323|emb|CAH67339.1| OSIGBa0157A06.8 [Oryza sativa Indica Group] &gt; gi|116310765|emb|CAH67558.1| OSIGBa0101P20.1 [Oryza sativa Indica Group] &gt; gi|125548968|gb|EAY94790.1| hypothetical protein OsI_16569 [Oryza sativa Indica Group] &gt; gi|125590941|gb|EAZ31291.1| hypothetical protein OsJ_15397 [Oryza sativa Japonica Group] BAJ85303 predicted protein [Hordeum vulgare subsp. 0.78 3064 3300 vulgare] Predicted 55-75 NP_001167787 hypothetical protein LOC100381480 [Zea 1.00 3065 3301 siRNA mays] &gt; gi|223943955|gb|ACN26061.1| 59235 unknown [Zea mays] NP_001143285 hypothetical protein LOC100275819 [Zea 0.97 3066 3302 mays] &gt; gi|195617040|gb|ACG30350.1| hypothetical protein [Zea mays] Predicted 512-531 NP_001149853 LOC100283481 [Zea mays] 1.00 3067 3303 siRNA &gt; gi|195635075|gb|ACG37006.1|pollen- 60123 specific protein like [Zea mays] NP_001152271 pollen-specific protein like [Zea mays] 0.83 3068 3304 &gt; gi|195638358|gb|ACG38647.1|pollen- specific protein like [Zea mays] &gt; gi|195642016|gb|ACG40476.1|pollen- specific protein like [Zea mays] &gt; gi|195654521|gb|ACG46728.1|pollen- specific protein like [Zea mays] XP_002457577 hypothetical protein 0.71 3069 3305 SORBIDRAFT_03g009730 [Sorghum bicolor] &gt; gi|241929552|gb|EES02697.1| hypothetical protein SORBIDRAFT_03g009730 [Sorghum bicolor] Predicted 1216-1237 XP_002455312 hypothetical protein 1.00 3070 3306 siRNA SORBIDRAFT_03g008220 [Sorghum 56837 bicolor] &gt; gi|241927287|gb|EES00432.1| hypothetical protein SORBIDRAFT_03g008220 [Sorghum bicolor] NP_001145952 hypothetical protein LOC100279478 [Zea 0.88 3071 3307 mays] &gt; gi|219885097|gb|ACL52923.1| unknown [Zea mays] NP_001143749 hypothetical protein LOC100276506 [Zea 0.89 3072 3308 mays] &gt; gi|195626214|gb|ACG34937.1| hypothetical protein [Zea mays] &gt; gi|195645208|gb|ACG42072.1| hypothetical protein [Zea mays] NP_001131959 hypothetical protein LOC100193355 [Zea 0.84 3073 3309 mays] &gt; gi|194693040|gb|ACF80604.1| unknown [Zea mays] NP_001041811 Os01g0112100 [Oryza sativa Japonica 0.72 3074 3310 Group] &gt; gi|113531342|dbj|BAF03725.1| Os01g0112100 [Oryza sativa Japonica Group] &gt; gi|125568749|gb|EAZ10264.1| hypothetical protein OsJ_00099 [Oryza sativa Japonica Group] &gt; gi|215697848|dbj|BAG92041.1| unnamed protein product [Oryza sativa Japonica Group] &gt; gi|215704817|dbj|BAG94845.1| unnamed protein product [Oryza sativa Japonica Group] EAY72253 hypothetical protein OsI_00107 [Oryza 0.72 3075 sativa Indica Group] Predicted 231-252 NP_001149348 serine carboxypeptidase 1 [Zea mays] 1.00 3076 3311 siRNA &gt; gi|195626594|gb|ACG35127.1|serine 55404 carboxypeptidase 1 precursor [Zea mays] NP_001137115 hypothetical protein LOC100217293 [Zea 0.98 3077 3312 mays] &gt; gi|194698414|gb|ACF83291.1| unknown [Zea mays] XP_002459781 hypothetical protein 0.72 3078 3313 SORBIDRAFT_02g010510 [Sorghum bicolor] &gt; gi|241923158|gb|EER96302.1| hypothetical protein SORBIDRAFT_02g010510 [Sorghum bicolor] 1046-1067 NP_001137115 hypothetical protein LOC100217293 [Zea 1.00 3079 3314 mays] &gt; gi|194698414|gb|ACF83291.1| unknown [Zea mays] NP_001149348 serine carboxypeptidase 1 [Zea mays] 0.99 3080 3315 &gt; gi|195626594|gb|ACG35127.1|serine carboxypeptidase 1 precursor [Zea mays] XP_002459781 hypothetical protein 0.72 3081 3316 SORBIDRAFT_02g010510 [Sorghum bicolor] &gt; gi|241923158|gb|EER96302.1| hypothetical protein SORBIDRAFT_02g010510 [Sorghum bicolor] Table 8: Provided are the target Genes of siRNAs Associated with Increased NUE (Table 4) along with their GenBank Accession numbers and sequence identifiers (SEQ ID NO:). “bind” = binding; “pos” = position; “hom” = homologue; “p.p.” = polypeptide; “p.n.” = polynucleotide.

TABLE 9 Target genes of mir395, 397 and 398 to be downregulated Hom. p.p. p.n. Mir NCBI SEQ SEQ Mir Bind. Access ID ID name Pos. ion Annotation/Organism Identity NO: NO: zma-miR3 322-342 YP_003208225 ribosomal protein S3 [Coix lacryma-jobi] 1.00 982 1647 98b* & gt;gi|209361951|gb|ACI43310.1| ribosomal protein S3 [Coix lacryma-jobi] NP_043062 ribosomal protein S3 [Zea mays] 0.99 983 1648 & gt;gi|48478711|ref|YP_024318.1| ribosomal protein S3 [Saccharum hybrid cultivar SP-80-3280] & gt;gi|50812566 |ref|YP_054668.1| ribosomal protein S3 [Saccharum officinarum] & gt;gi|118614530|ref|YP_899446.1 ribosomal protein S3 [Sorghum bicolor] & gt;gi|33933|sp|P06586.1|RR3_MAIZE RecName: Full = 30S ribosomal protein S3, chloroplastic & gt;gi|68052946|sp|Q6ENS5.1|RR3_SACOF RecName: Full = 30S ribosomal protein S3, chloroplastic & gt;gi|75126330|sp|Q6L3G0.1|RR3_SACHY RecName: Full = 30S ribosomal protein S3, chloroplastic & gt;gi|125987748|sp|A1E9W3.1|RR3_SORBI RecName: Full = 30S ribosomal protein S3, chloroplastic & gt;gi|12469|emb|CAA68427.1|ribosomal protein S3 [Zea mays] & gt;gi|902260|emb|CAA60324.1|ribosomal protein S3 [Zea mays] & gt;gi|48478613& gt;gi|AAT44633.1 ribosomal protein S3 [Saccharum hybrid cultivar SP80-3280] & gt;gi|49659550|dbj|BAD27331.1 ribosomal protein S3 [Saccharum hybrid & gt;gi|11820164|gb|ABK79534.1 ribosomal protein S3 [Sorghum bicolor] ADN86110 ribosomal protein S3 [Chasmanthium latifolium] 0.96 984 1649 ADN32929 ribosomal protein S3 [Phyllostachys nigra var. henonis] 0.96 985 & gt;gi|309321655|gb|ADO65180.1 ribosomal protein S3 [Acidosasa purpurea] & gt;gi|309321739|gb|ADO65263.1 ribosomal protein S3 [Ferrocalamus rimosivaginus] & gt;gi|309321823|gb|ADO65346.1 ribosomal protein S3 [Indocalamus longiauritus] & gt;gi|309321906|gb|ADO65428.14 ribosomal protein S3 [Phllostachys edulis] YP_003097613 ribosomal protein S3 [Dendrocalamus latiflorus] 0.96 986 1650 & gt;gi|255040297|gb|ACT99957.1 ribosomal protein S3 [Dendrocalamus latisflorus] ADD63064 ribosomal protein S3 [Potamophila parviflora] 0.96 987 1651 ADD62997 ribosomal protein S3 [Ortza australiensis] 0.96 988 1652 NP_039424 ribosomal protein S3 [Ortza sativa Japonica Group] 0.96 989 1653 & gt;gi|50234010|ref|YP_052788.1 ribosomal protein S3 [Ortza nivara] & gt;gi|09156623|ref|YP_654242.1 ribosomal protein S3 [Oryza sativa Indica Group] & gt;gi|68052945|sp|Q6END5.1|RR3_ORYNI RecName: Full = 30S ribosomal protein S3, chloroplastic & gt;gi|48840859|sp|P0C483.1| RR3_ORYSA RecName: Full = 30S ribosomal protein S3, chloroplastic & gt;gi| 148840860|sp|P0C484.1|RR3_ORYSI RecName: Full = 30S ribosomal protein S3, chloroplastic & gt;gi|148840861|sp|P0C485.1|RR3_ORYSA RecName: Full = 30S ribosomal protein S3, chloroplastic & gt;gi|l2025|emb|CAA33934.1 ribosomal protein S3 [Ortza sativa Japonica Group] & gt;gi|42795515|gb|AAS46082.1 ribosomal protein S3 [Ortza sativa Indica Group] & gt;gi|42795581|gb|AAS46147.1 ribosomal protein S3 [Ortza sativa Japonica Group] & gt;gi|4279564|gb|AAS46210.1 ribosomal protein S3 [Ortza sativa Japonica Group] & gt;gi|49615034|dbj|BAD26817.1 ribosomal protein S3 [Ortza nivara] & gt;gi|50725165|dbj|BAD33782.1 ribosomal protein S3 [Ortza sativa Japonica Group] & gt;gi|l51091521|dbj|BAD36259.1 ribosomal protein S3 [Ortza sativa Japonica Group] & gt;gi|8200203|gb|EEC82630.1 hypothetical protein OsI_27221 [Oryza sativa Indica Group] & gt;gi|218202023|gb|EEC84450.1 hypothetical protein OsI_31072 [Oryza sativa Indica Group] & gt;gi|290790601|gb|ADD62861.1 ribosomal protein S3 [Ortza sativa Japonica Group] & gt;gi|290790670|gb|ADD62929.1 ribosomal protein S3 [Oryza meridionalis] & gt;gi|226646|prf| 1603356BW ribosomal protein S3 ADO65512 ribosomal protein S3 [Bambusa emeiensis] 0.95 990 BAD05516 ribosomal protein S3 [Ortza sativa Japonica Group] 0.95 991 1654 osa- 426-446 XP_002463896 hypothetical protein SORBIDRAFT_01g008450 1.00 1249 1889 miR395m [Sorghum bicolor] & gt;gi|241917750|gb|EER90894.1 SORBIDRAFT_01g008450 [Sorghum bicolor] ACN28609 unknown [Zea mays] 0.95 1250 1890 ACN34023 unknown [Zea mays] 0.95 1251 1891 ACG45192 bifunctional 3-phosphoadenosine 5- 0.94 1252 1892 phosphosulfate synthtase [Zea mays] NP_001104877 ATP sulfurylase [Zea mays] & gt;gi|2738750|gb|AAB94542.1 0.95 1253 1893 ATP sulfurylase [Zea mays] EAY91825 hypothetical protein OsI_13470 [Oryza sativa Indica Group] 0.80 1254 NP_001051234 Os03g0743900 [Oryza sativa Japonica Group] 0.80 1255 1894 & gt;gi|30017582|gb|AAP13004.1 putative ATP sulfurylase [Oryza sativa Japonica Group] & gt;gi|108711024|gb|ABF98819.1 Bifunctional 3 & apos;-phosphoadenosine 5 & apos;-phosphosulfate synthethase, putative, expressed [Oryza sativa Japonica Group] & gt;gi|113549705|dbj|BAF13148.1 Os03g0743900 [Oryza sativa Japonica Group] & gt;gi|215704581|dbj|BAG94214.1 unnamed protein product [Oryza sativa Japonica Group] BAK05662 predicted protein [Hordeum vulgare subsp. vulgare] 0.79 1256 1895 & gt;gi|326502564|dbj|BAJ95345.1 predicted protein [Hordeum vulgare subsp. vulgare] BAA36274 plastidic ATP sulfurylase [Oryza sativa Indica Group] 0.80 1257 1896 EAZ28548 hypothetical protein OsJ_12530 [Oryza sativa Japonica Group] 0.77 1258 352-372 XP_002441904 hypothetical protein SORBIDRAFT_08g004650 1.00 1259 1897 [Sorghum bicolor] & gt;gi|241942597|gb|EES15742.1 hypothetical protein SORBIDRAFT_08g004650 [Sorghum bicolor] EEE52851 hypothetical protein OsJ_35390 [Oryza sativa Japonica Group] 0.71 1260 NP_001066285 Os12g0174100 [Oryza sativa Japonica Group] 0.71 1261 1898 & gt;gi|77553790|gb|ABA96586.1 Growth regulator protein, putative, expressed [Oryza sativa Japonica Group] & gt;gi| 255670095|dbj|BAF29304.2 Os12g0174100 [Oryza sativa Japonica Group] EEC68940 hypothetical protein OsI_37646 [Oryza sativa Indica Group] 0.70 1262 528-548 ACN34023 unknown [Zea mays] 1.00 1263 1899 NP_001104877 ATP sulfurylase [Zea mays] & gt;gi|2738750|gb|AAB94542.1 0.98 1264 1900 ATP sulfurylase [Zea mays] ACN28609 unknown [Zea mays] 0.94 1265 1901 ACG41592 bifunctional 3-phosphoadenosine 5- 0.94 1266 1902 phosphosulfate synthetase [Zea mays] XP_002463896 hypothetical protein SORBIDRAFT_01g008450 0.94 1267 1903 [Sorghum bicolor] & gt;gi|241917750|gb|EER90894.1 hypothetical protein SORBIDRAFT_01g008450 [Sorghum bicolor] BAK05662 predicted protein [Hordeum vulgare subsp. vulgare] 0.84 1268 1904 & gt;gi|326502564|dbj|BAJ95345.1 predicted protein [Hordeum vulgare subsp. vulfare] EAY91825 hypothetical protein OsI_13470 [Oryza sativa Indica Group] 0.80 1269 NP_001051234 Os03g0743900 [Oryza sativa Japonica Group] 0.79 1270 1905 & gt;gi|30017582|gb|AAP13004.1 putative ATP sulfurylase [Oryza sativa Japonica Group] & gt;gi|108711024|gb|ABF98819.1 Bifunctional 3 & apos;-phosphoadenosine 5 & aps;-phosphosulfate synthethase, putative, expressed [Oryza sativa Japonica Group] & gt;gi|13549705|dbj| BAF13148.1Os03g0743900 [Oryza sativa Japonica Group] & gt;gi|215704581|dbj|BAG94214.1 unnamed protein product [Oryza sativa Japonica Group] BAA36274 plastidic ATP sulfurylase [Oryza sativa Indica Group] 0.79 1271 1906 EAZ28548 hypothetical protein OsJ_12530 [Oryza sativa Japonica Group] 0.76 1272 305-325 ACL53345 unknown [Zea mays] 1.00 1273 1907 XP_002465703 hypothetical protein SORBIDRAFT_01g044100 [Sorghum bicolor] 0.95 1274 1908 & gt;gi|241919557|gb|EER92701.1 hypothetical protein SORBIDRAFT_01g044100 [Sorghum bicolor] BAJ85215 predicted protein [Hordeum vulgare subsp. vulgare] 0.85 1275 1909 & gt;gi|326511599|dbj|BAJ91944.1 predicted protein [Hordeum vulgare subsp. vulfare] & gt;gi|25585257|gb|EAZ25921.1 hypothetical protein OsJ_09764 [Oryza sativa Japonica Group] AAN59769 Putative sulfate transporter [Oryza sativa Japonica Group] 0.85 1276 & gt;gi|125585257|gb|EAZ25921.1| hypothetical protein OsJ_09764 [Oryza sativa Japonica Group] EEC74682 hypothetical protein OsI_10373 [Oryza sativa Japonica Group] 0.85 1277 EEC74681 hypothetical protein OsI_10372 [Oryza sativa Japonica Group] 0.72 1278 EEE58498 hypothetical protein OsJ_09763 [Oryza sativa Japonica Group] 0.72 1279 NP_001049257 Os03g0195300 [Oryza sativa Japonica Group] 0.72 1280 1910 & gt;gi|108706649|gb|ABF94444.1 Sulfate transporter 2.1, putative, expressed [Oryza sativa Japonica Group] & gt;gi|13547728|dbj|BAF11171.1 Os03g0195300 [Oryza sativa Japonica Group] zma- 798-818 XP_002458747 hypothetical protein SORBIDRAFT_03g039530 [Sorghum bicolor] 1.00 1321 1949 miR397a & gt;gi|241930722|gb|EES03867.1 hypothetical protein SORBIDRAFT_03g039530 [Sorghum bicolor] NP_001147942 L-ascorbate oxidase [Zea mays] & gt;gi|195614732|gb|ACG29196.1 0.96 1322 1950 L-ascorbate oxidase precursor [Zea mays] ACN34362 unknown [Zea mays] 0.96 1323 1951 EEC71780 hypothetical protein OsI_04394 [Oryza sativa Indica Group] 0.86 1324 NP_001044773 Os03g0842500 [Oryza sativa Japonica Group] 0.86 1325 1952 & gt;gi|19571025|gb|BAB86452.1 putative laccase LAC5-6 [Oryza sativa Japonica Group] & gt;gi|13534304|dbj|BAF06687.1 Os01g0842500 [Oryza sativa Japonica Group] & gt;gi|125572601|gb|EAZ14116.1 hypothetical protein OsJ_04040 [Oryza sativa Japonica Group] & gt;gi|21569481|dbj|NAG90005.1 unnamed protein product [Oryza sativa Japonica Group] BAJ84890 predicted protein [Hordeum vulgare subsp. vulgare] 0.84 1326 1953 BAJ96691 predicted protein [Hordeum vulgare subsp. vulgare] 0.84 1327 1954 NP_001105875 putative laccase [Zea mays] 0.79 1328 1955 & gt;gi|8461878|emb|CAJ30500.1 putative laccase [Zea mays] ACN28855 unknown [Zea mays] 0.79 1329 1956 XP_002458746 hypothetical protein SORBIDRAFT_03g039520 0.78 1330 1957 [Sorghum bicolor] & gt;gi|241930721|gb|EES03866.1 hypothetical protein SORBIDRAFT_03g039520 [Sorghum bicolor] 458-478 ACF87912 unknown [Zea mays] 1.00 1331 1958 NP_001105951 LOC100037774 [Zea mays] & gt;gi|1184776|gb|AAA87580.1 0.99 1332 1959 cytosolic glyceraldehyde-3-phosphate dehydrogenase GAPC4 [Zea mays] NP_001105385 glyceraldehyde-3-phosphate dehydrogenase, 0.99 1333 1960 cytosolic 3 [Zea mays] & gt;gi|6166167|sp|Q43247.1G3PE_MAIZE RecName: Full = Glyceraldehyde-3-phosphate dehydrogense, cytosolic 3 & gt;gi|184774|gb|AAA87579.1 cytosolic glyceraldehyde-3-phosphate dehydrogenase GAPC3 [Zea mays] ACG36109 glyceraldehyde-3-phosphate dehydrogenase, 0.99 1334 1961 cytosolic 3 [Zea mays] ADZ55283 glyceraldehyde-3-phosphate dehydrogenase [Zea mays] 0.98 1335 1962 XP_002452401 hypothetical protein SORBIDRAFT_04g025120 0.98 1336 1963 [Sorghum bicolor] & gt;gi|241932232|gb|EES05377.1 hypothetical protein SORBIDRAFT_04g025120 [Sorghum bicolor] NP_001053139 Os04g0486600 [Oryza sativa Japonica Group] 0.92 1337 1964 & gt;gi|3834606|emb|CAE02009.2 OJ0002233_09.15 [Oryza sativa Japonica Group] & gt;gi|90265255|emb|CAD79700.2H0302E05.3 [Oryza sativa Japonica Group] & gt;gi|13564710|dbj|BAF15053.1 Os05g0486600 [Oryza sativa Japonica Group] & gt;gi|69244439|gb|ACA50493.1 glyceraldehyde- 3-phosphate dehydrogenase [Oryza sativa Japonica Group] & gt;gi|215694303|dbj|BAG89296.1 unnamed protein product [Oryza sativa Japonica Group] & gt;gi|306415975|gb|ADM86862.1 glyceraldehyde-3-phosphate dehydrogenase [Oryza sativa Japonica Group] NP_001047348 Os02g0601300 [Oryza sativa Japonica Group] 0.92 1338 1965 & gt;gi|47848293|dbj|BAD22157.1 putative glyceraldehyde-3-phosphate dehydrogenase (phosporylating) [Oryza sativa Japonica Group] & gt;gi|13536879|dbj|BAF09262.1 Os02g0601300 [Oryza sativa Japonica Group] & gt;gi|119434402|gb|ABL75274.1 glyceralde-3- phosphate dehydrogenase [Oryza sativa] & gt;gi|215704481|dbjBAG93915.1| unnamed protein product [Oryza sativa Japonica Group] & gt;gi|306415941|gb|ADM86845.1 glyceraldehyde- 3-phosphate dehydrogenase [Oryza sativa Japonica Group] EEC73541 hypothetical protein OsI_07948 [Oryza sativa Indica Group] 0.91 1339 ABQ81648 glyceraldehyde-3-phosphate dehydrogenase [Triticum aestivum] 0.91 1340 1966 201-221 XP_002458746 hypothetical protein SORBIDRAFT_03g039520 1.00 1341 1967 [Sorghum bicolor] & gt;gi|241930721|gb|EES03866.1 hypothetical protein SORBIDRAFT_03g039520 [Sorghum bicolor] NP_001105875 putative laccase [Zea mays] & gt;gi|84618781|emb|CAJ30500.1 0.94 1342 1968 putative laccase [Zea mays] NP_001146658 hypothetical protein LOC100280258 0.93 1343 1969 [Zea mays] & gt;gi|219888209|gb|ACL54479.1 unknown [Zea mays] ACN28855 unknown [Zea mays] 0.94 1344 1970 NP_001044772 Os01g0842400 [Ortza satuva Japonica Group] 0.80 1345 1971 & gt;gi|75321217|sp|Q5N9X2.1|LAC4_ORYSJ RecName: Full = Laccase-4; AltName: Full = Benzenediol: oxygen oxidoreductase 4; AltName: Full = Diphenol oxidase 4; AltName: Full = Urishiol oxidase 4; flags: precursor & gt;gi|56784239|dbj|BAD81734.1| putative laccase LAC5-6 [Oryza sativa Japonica Group] & gt;gi|13534303|dbj|BAF06686.1|Os01g0842400 [Oryza sativa Japonica Group] & gt;gi|l215697155 |dbj|BAG91149.1| unnamed protein product [Oryza sativa Japonica Group] EAZ14115 hypothetical protein OsJ_04039 [Oryza sativa Japonica Group] 0.80 1346 EEC71777 hypothetical protein OsJ_04389 [Oryza sativa Indica Group] 0.80 1347 BAJ99773 predicted protein [Hordeum vulgare subsp. vulgare) 0.78 1348 1972 XP_002458747 hypothetical protein SORBIDRAFT_03g039530 0.78 1349 1973 [Sorghum bicolor] & gt;gi|241930722|gb|EES03867.1 hypothetical protein SORBIDRAFT_03g039530 [Sorghum bicolor] AAC04576 putative high-pI laccase [Oryza sativa Japonica Group] 0.79 1350 1974 mtr- 426-446 XP_002463896 hypothetical protein SORBIDRAFT_01g008450 1.00 1351 1975 miR395c [Sorghum bicolor] & gt;gi|241917750|gb|EER90894.1 hypothetical protein SORBIDRAFT_01g008450 [Sorghum bicolor] ACN28609 unknown [Zea mays] 0.95 1352 1976 ACN34023 unknown [Zea mays] 0.95 1353 1977 ACG45192 bifunctional 3-phosphoadenosine 5-phosphosulfate 0.94 1354 1978 synthtase [Zea mays] NP_001104877 ATP sulfurylase [Zea mays] & gt;gi|2738750|gb|AAB94542.1 0.95 1355 1979 ATP sulfurylase [Zea mays] EAY91825 hypothetical protein OsI_13470 [Oryza sativa Indica Group] 0.80 1356 NP_001051234 Os03g0743900 [Oryza sativa Japonica Group] 0.80 1357 1980 & gt;gi|30017582|gb|AAP13004.1 putative ATP sulfurylase [Oryza sativa Japonica Group] & gt;gi|108711024|gb|ABF98819.1 Bifunctional 3 & apos;-phosphoadenenosine 5 & apos;- phosphosulfate synthethase, putative, expressed [Oryza sativa Japonica Group] & gt;gi|13549705|dbj|BAF13148.1 Os03g0743900 [Oryza sativa Japonica Group] & gt;gi|215704581|dbj| BAG94214.1 unnamed protein product [Oryza sativa Japonica Group] BAK05662 predicted protein [Hordeum vulfare subsp. vulgare] 0.79 1358 1981 & gt;gi|326502564|dbj|BAJ95345.1 predicted protein product [Hordeum vulgare subsp. vulgare] BAA36274 plastidic ATP sulfurylase [Oryza sativa Indica Group] 0.80 1359 1982 EAZ28548 hypothetical protein OsJ_12530 [Oryza sativa Japonica Group] 0.77 1360 528-548 ACN34023 unknown [Zea mays] 1.00 1361 1983 NP_001104877 ATP sulfurylase [Zea mays] & gt;gi|2738750|gb|AAB94542.1 0.98 1362 1984 ATP sulfurylase [Zea mays] ACN28609 unknown [Zea mays] 0.94 1363 1985 ACG45192 bifunctional 3-phosphoadenosine 0.94 1364 1986 5-phosphosulfate synthetase [Zea mays] XP_002463896 hypothetical protein SORBIDRAFT_01g008450 0.94 1365 1987 [Sorghum bicolor] & gt;gi|241917750|gb|EER90894.1 hypothetical protein SORBIDRAFT_01g008450 [Sorghum bicolor] BAK05662 predicted protein [Hordeum vulgare subsp. vulgare] 0.84 1366 1988 & gt;gi|326502564|dbj|BAJ95345.1 predicted protein [Hordeum vulgare subsp. vulgare] EAY91825 hypothetical protein OsI_13470 [Oryza sativa Indica Group] 0.80 1367 NP_001051234 Os03g0743900 [Oryza sativa Japonica Group] 0.79 1368 1989 & gt;gi|30017582|gb|AAP13004.1 putative ATP sulfurylase [Oryza sativa Japonica Group] & gt;gi|10871102|gb|ABF98819.1 Bifunctional 3 & apos;- phosphoadenosine 5 & apos;-phosphosulfate synthethase, putative, expressed [Oryza sativa Japonica Group] & gt;gi|113549705|dbj|BAF13148.1Os03g0743900 [Oryza sativa Japonica Group] & gt;gi|215704581|dbj|BAG94214.1 unnamed protein product [Oryza sativa Japonica Group] BAA36274 plastidic ATP sulfurylase [Oryza sativa Indica Group] 0.79 1369 1990 EAZ28548 hypothetical protein OsJ_12530 [Oryza sativa Japonica Group] 0.76 1370 359-379 XP_002452295 hypothetical protein SORBIDRAFT_04g023180 1.00 1371 1991 [Sorghum bicolor] & gt;gi|241932126|gb|EES05271.1 hypothetical protein SORBIDRAFT_04g023180 [Sorghum bicolor] NP_001148591 2-hydrozy-3-oxopropionate reductase 0.98 1372 1992 [Zea mays] & gt;gi|95620656|gb|ACG32158.1 2-hydrozy-3-oxopropionate reductase [Zea mays] NP_001047154 Os02g0562700 [Oryza sativa Japonica Group] 0.92 1373 1993 & gt;gi|29368238|gb|AAAO72678.1 gamma hydroxybutyrate dehydrogenase-like protein [Oryza sativa Japonica Group] & gt;gi|32352132|dbj|BAC78559.1 hypothetical protein [Oryza sativa Japonica Group] & gt;gi|46390142|dbj|BAD15576.1 putative gamma hydroxybutyrate dehydrogenase [Oryza sativa Japonica Group] & gt;gi|13536685|dbj| BAF09068.1|Os02g562700 [Oryza sativa Japonica Group] & gt;gi|215679041|dbj|BAG96471.1 unnamed protein product [Oryza sativa Japonica Group] & gt;gi|21501279|dbj|BAG92703.1 unnamed protein product [Oryza sativa Japonica Group] & gt;gi|215766587|dbj|BAG98746.1 unnamed protein product [Oryza sativa Japonica Group] & gt;gi|l218190991|gb|EEC73418.1 hypothetical protein OsI_07685 [Oryza sativa Indica Group] & gt;gi|222623072|gb|EEE57204.1 hypothetical protein OsJ_07161 [Oryza sativa Japonica Group] BAJ91168 predicted protein [Hordeum vulgare subsp. vulgare] 0.90 1374 1994 & gt;gi|326518654|dbj|BAJ88356.1 predicted protein [Hordeum vulgare subsp. vulgare] XP_002266252 PREDICTED: hypothetical protein isoform 1 0.80 1375 1995 [Vitis vinifera] & gt;gi|47861759|emb|CAN78910.1 hypothetical protein VITISV_032948 [Vitis vinifera] & gt;gi|297736660|emb|CBI25677.3 unnamed protein product [Vitis vinifera] ACU2717 unknown [Glycine max] 0.80 1376 1996 XP_002266296 PREDICTED: hypothetical protein isoform 2 [Vitis vinifera] 0.78 1377 1997 XP_002320548 predicted protein [Populus trichocarpa] 0.76 1378 1998 & gt;gi|222861321|gb|EEE98863.1 predicted protein [Populus trichocarpa] BAG16485 succinic semialdehyde reductase isofom1 [Solanum lycopersicum] 0.79 1379 1999 ABK22179 unknown [Picea sitchensis] 0.77 1380 2000 305-325 ACL53345 unknown [Zea mays] 1.00 1381 2001 XP_002465703 hypothetical protein SORBIDRAFT_01g044100 0.95 1382 2002 [Sorghum bicolor] & gt;gi|241919557|gb|EER92701.1 hypothetical protein SORBIDRAFT_01g044100 [Sorghum bicolor] BAJ85215 predicted protein [Hordeum vulgare subsp. vulgare] 0.85 1383 2003 & gt;gi|326511599|dbj|BAJ91944.1 predicted protein [Hordeum vulgare subsp. vulgare] AAN59769 Putative sulfate transporter [Oryza sativa Japonica Group] 0.85 1384 & gt;gi|25585257|gb|EAZ25921.1 hypothetical protein OsJ_09764 [Oryza sativa Japonica Group] EEC74682 hypothetical protein OsJ_10373 [Oryza sativa Indica Group] 0.85 1385 EEC74681 hypothetical protein OsJ_10372 [Oryza sativa Indica Group] 0.72 1386 EEE58498 hypothetical protein OsJ_09763[Oryza sativa Japonica Group] 0.72 1387 NP_001049257 Os03g0195300 [Oryza sativa Japonica Group] 0.72 1388 2004 & gt;gi|108706649|gb|ABF94444.1 Sulfate transporter 2.1, putative, expressed [Oryza sativa Japonica Group] & gt;gi|13547728|dbj|BAF11171.1Os03g0195300 [Oryza sativa Japonica Group] Predicted 201-221 XP_002456833 hypothetical protein SORBIDRAFT_03g043750 1.00 1311 1939 zmamir [Sorghum bicolor] & gt;gi|241928808|gb|EES01953.1 50266 hypothetical protein SORBIDRAFT_03g043750 [Sorghum bicolor] NP_001131338 hypothetical protein LOC100192654 0.96 1312 1940 [Zea mays] & gt;gi|94690154|gb|ACF79161.1 unknown [Zea mays] & gt;gi|219884631|gb|ACL52690.1 unknown [Zea mays] & gt;gil238908563|gb|ACF79698.2unknown [Zea mays] ACL52654 unknown [Zea mays] 0.92 1313 1941 ACG35805 selenium-binding protein [Zea mays] 0.92 1314 1942 NP_001045194 Os01g0916400 [Oryza sativa Japonica Group] 0.90 1315 1943 & gt;gi|19386752|dbj|BAB86133.1 putative selenium binding protein [Oryza sativa Japonica Group] & gt;gi|20805006|dbj|BAB92682.1 putative selenium binding protein [Oryza sativa Japonica Group] & gt;gi|31322241|gb|AAO91777.1 putative selenium binding protein [Oryza sativa Japonica Group] & gt;gi|113534725|dbj| BAF07108.1|Os01g0916400 [Oryza sativa Japonica Group] & gt;gi|125528845|gb|EAY76959.1| hypothetical protein OsI_04917 [Oryza sativa Indica Group] & gt;gi|25573100|gb|EAZ14615.1| hypothetical protein OsJ_04540 [Oryza sativa Japonica Group] & gt;gi|13534725|dbj|BAF07108.1OS01g0916400 [Oryza sativa Japonica Group] BAJ94729 predicted protein [Hordeum vulgare subsp. vulgare] 0.88 1316 1944 BAB40923 putative selenium binding protein 0.88 1317 1945 [Oryza sativa (japonica cultivar-group)] XP_002328915 predicted protein [Populus trichocarpa] 0.80 1318 1946 & gt;gi|222839345|gb|EEE77682.1 predicted protein [Populus trichocarpa] XP_002298672 predicted protein [Populus trichocarpa] 0.80 1319 1947 & gt;gi|222845930|gb|EEE3477.1 predicted protein [Populus trichocarpa] XP_002520613 selenium-binding protein, putative [Ricinus communis] 0.80 1320 1948 & gt;gi|223540174|gb|EEF41749.1 selenium-binding protein, putative [Ricinus communis] Table 9, provided are target genes to be downregulated, of mirs 395, 397 and 398.

TABLE 10 Abbreviations of plant species Provided are the abbreviations and full names of plant species. Abbreviation Organism Name Common Name ahy Arachis hypogaea Peanut aly Arabidopsis lyrata Arabidopsis lyrata aqc Aquilegia coerulea Rocky Mountain Columbine ata Aegilops taushii Tausch's goatgrass ath Arabidopsis thaliana Arabidopsis thaliana bdi Brachypodium distachyon Grass bna Brassica napus Brassica napus canola (“liftit”) bol Brassica oleracea Brassica oleracea wild cabbage bra Brassica rapa Brassica rapa yellow mustard ccl Citrus clementine Clementine csi Citrus sinensis Orange ctr Citrus trifoliata Trifoliate orange gma Glycine max Glycine max gso Glycine soja Wild soybean hvu Hordeum vulgare Barley lja Lotus japonicus Lotus japonicus mtr Medicago truncatula Medicago truncatula —Barrel Clover (“tiltan”) osa Oryza sativa Oryza sativa pab Picea abies European spruce ppt Physcomitrella patens Physcomitrella patens (moss) pta Pinus taeda Pinus taeda —Loblolly Pine ptc Populus trichocarpa Populus trichocarpa —black cotton wood rco Ricinus communis Castor bean (“kikayon”) sbi Sorghum bicolor Sorghum bicolor Dura sly Solanum lycopersicum tomato microtom smo Selaginella moellendorffii Selaginella moellendorffii sof Saccharum officinarum Sugarcane ssp Saccharum spp Sugarcane tae Triticum aestivum Triticum aestivum tcc Theobroma cacao cacao tree and cocoa tree vvi Vitis vinifera Vitis vinifera Grapes zma Zea mays corn

Example 3 Gene Cloning and Creation of Binary Vectors for Plant Expression

The predicted target sequences were cloned into binary vectors for the generation of transgenic plants. The full-length open reading frame (ORF) was synthesized by Genscript (Israel). The resultant clone was digested with appropriate restriction enzymes and inserted into the Multi Cloning Site (MCS) of a similarly digested binary vector through ligation using T4 DNA ligase enzyme (Promega, Madison, Wis., USA). FIG. 1 is a plasmid map of the binary vector pORE-E1, used for plant transformation.

Example 4 Generation of Transgenic Model Plants Expressing the Target Genes/Silencing Agents of Some Embodiments of the Invention

Arabidoposis thaliana transformation was performed using the floral dip procedure following a slightly modified version of the published protocol (Clough and Bent, 1998, Plant J 16(6): 735-43; Desfeux et al, 2000, Plant Physiol. 123(3): 895-904). Briefly, T₀ Plants were planted in small pots filled with soil. The pots were covered with aluminum foil and a plastic dome, kept at 4° C. for 3-4 days, then uncovered and incubated in a growth chamber at 24° C. under 16 hr light:8 hr dark cycles. A week prior to transformation all individual flowering stems were removed to allow for growth of multiple flowering stems instead. A single colony of Agrobacterium (GV3101) carrying the binary vectors (pORE-E1), harboring the target gene or silencing sequence was cultured in LB medium supplemented with kanamycin (50 mg/L) and gentamycin (25 mg/L). Three days prior to transformation, each culture was incubated at 28° C. for 48 hrs, shaking at 180 rpm. The starter culture was split the day before transformation into two cultures, which were allowed to grow further at 28° C. for 24 hours at 180 rpm. Pellets containing the agrobacterium cells were obtained by centrifugation of the cultures at 5000 rpm for 15 minutes. The pellets were resuspended in an infiltration medium (10 mM MgCl₂, 5% sucrose, 0.044 μM BAP (Sigma) and 0.03% Tween 20) in double-distilled water.

Transformation of T₀ plants was performed by inverting each plant into the Agrobacterium suspension, keeping the flowering stem submerged for 5 minutes. Following inoculation, each plant was blotted dry for 5 minutes on both sides, and placed sideways on a fresh covered tray for 24 hours at 22° C. Transformed (transgenic) plants were then uncovered and transferred to a greenhouse for recovery and maturation. The transgenic T₀ plants were grown in the greenhouse for 3-5 weeks until the seeds are ready. The seeds were then harvested from plants and kept at room temperature until sowing.

Example 5 Selection of Transgenic Arabidopsis Plants Expressing the Nucleic Acid Molecules of Some Embodiments of the Invention According to Expression Level

Arabidopsis seeds were sown. One to 2 weeks old seedlings were sprayed with a non-volatile herbicide, Basta (Bayer) at least twice every few days. Only resistant plants, which are heterozygous for the transgene, survive. PCR on the genomic gene sequence was performed on the surviving seedlings using primers pORE-F2 (fwd, 5′-TTTAGCGATGAACTTCACTC-3′/SEQ ID NO:310) and a custom designed reverse primer based on each target sequence.

Example 6 Evaluating Changes in Root Architecture in Transgenic Plants

Root architecture of the plant governs multiple key agricultural traits. Root size and depth have been shown to logically correlate with drought tolerance and enhanced NUE, since deeper and more branched root systems provide better soil coverage and can access water and nutrients stored in deeper soil layers.

To test whether the transgenic plants produce a modified root structure, plants were grown in agar plates placed vertically. A digital picture of the plates was taken every few days and the maximal length and total area covered by the plant roots were assessed. From every construct created, several independent transformation events were checked in replicates. To assess significant differences between root features, statistical test, such as a Student's t-test, was employed in order to identify enhanced root features and to provide a statistical value to the findings.

Example 7 Testing for Increased Nitrogen Use Efficiency (NUE)

To analyze whether the transgenic Arabidopsis plants are more responsive to nitrogen, plants were grown in two different nitrogen concentrations: (1) optimal nitrogen concentration (100% NH₄NO₃, which corresponds to 20.61 mM) or (2) nitrogen deficient conditions (1% or 10% NH₄NO₃, which corresponds to 0.2 and 2.06 mM, respectively). Plants were allowed to grow until seed production followed by an analysis of their overall size, time to flowering, yield, protein content of shoot and/or grain, and seed production. The parameters checked are each of the overall size of the plant, wet and dry weight, the weight of the seeds yielded, the average seed size and the number of seeds produced per plant. Other parameters that are tested include: the chlorophyll content of leaves (as nitrogen plant status and the degree of leaf greenness are highly correlated), amino acid and the total protein content of the seeds or other plant parts such as leaves or shoots and oil content. Transformed plants not exhibiting substantial physiological and/or morphological effects, or exhibiting higher measured parameters levels than wild-type plants, are identified as nitrogen use efficient plants.

Although the invention has been described in conjunction with specific embodiments thereof, it is evident that many alternatives, modifications and variations will be apparent to those skilled in the art. Accordingly, it is intended to embrace all such alternatives, modifications and variations that fall within the spirit and broad scope of the appended claims.

All publications, patents and patent applications mentioned in this specification are herein incorporated in their entirety by reference into the specification, to the same extent as if each individual publication, patent or patent application was specifically and individually indicated to be incorporated herein by reference. In addition, citation or identification of any reference in this application shall not be construed as an admission that such reference is available as prior art to the present invention. To the extent that section headings are used, they should not be construed as necessarily limiting. 

What is claimed is:
 1. A method of improving nitrogen use efficiency, abiotic stress tolerance, biomass, vigor or yield of a plant, the method comprising expressing within the plant an exogenous polynucleotide which downregulates an activity or expression of a polypeptide having an amino acid sequence 100% homologous to SEQ ID NOs: 312 and 317, wherein said polypeptide is capable of regulating nitrogen use efficiency of the plant, thereby improving nitrogen use efficiency, abiotic stress tolerance, biomass, vigor or yield of the plant.
 2. A transgenic plant exogenously expressing a polynucleotide which downregulates an activity or expression of a polypeptide having an amino acid sequence 100% homologous to SEQ ID NOs: 312 and 317, wherein said polypeptide is capable of regulating nitrogen use efficiency of the plant.
 3. A nucleic acid construct comprising a polynucleotide which downregulates an activity or expression of a polypeptide having an amino acid sequence 100% homologous to SEQ ID NOs: 312 and 317, wherein said polypeptide is capable of regulating nitrogen use efficiency of a plant, said nucleic acid sequence being under the regulation of a cis-acting regulatory element.
 4. The method of claim 1, the transgenic plant of claim 2 or the nucleic acid construct of claim 3, wherein said polynucleotide acts by a mechanism selected from the group consisting of sense suppression, antisense suppression, ribozyme inhibition, gene disruption.
 5. The nucleic acid construct of claim 3, wherein said cis-acting regulatory element comprises a promoter.
 6. The nucleic acid construct of claim 5, wherein said promoter comprises a tissue-specific promoter.
 7. The nucleic acid construct of claim 6, wherein said tissue-specific promoter comprises a root specific promoter.
 8. The method of claim 1, further comprising growing the plant under limiting nitrogen conditions.
 9. The method of claim 1, further comprising growing the plant under abiotic stress.
 10. The method of claim 9, wherein said abiotic stress is selected from the group consisting of salinity, drought, water deprivation, flood, etiolation, low temperature, high temperature, heavy metal toxicity, anaerobiosis, nutrient deficiency, nutrient excess, atmospheric pollution and UV irradiation.
 11. The method of claim 1 wherein the plant is a monocotyledon.
 12. The method of claim 1, wherein the plant is a dicotyledon. 